Genshi Zhao

Summary

Affiliation: Eli Lilly and Company
Country: USA

Publications

  1. ncbi request reprint Biochemical and molecular analyses of the C-terminal domain of Era GTPase from Streptococcus pneumoniae
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Infectious Diseases Research, Indianapolis, IN 46285 0438, USA
    Microbiology 145:791-800. 1999
  2. pmc Biochemical characterization of penicillin-resistant and -sensitive penicillin-binding protein 2x transpeptidase activities of Streptococcus pneumoniae and mechanistic implications in bacterial resistance to beta-lactam antibiotics
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285 0438, USA
    J Bacteriol 179:4901-8. 1997
  3. pmc BOCILLIN FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285 0438, USA
    Antimicrob Agents Chemother 43:1124-8. 1999
  4. ncbi request reprint Penicillin-binding protein 2a of Streptococcus pneumoniae: expression in Escherichia coli and purification and refolding of inclusion bodies into a soluble and enzymatically active enzyme
    G Zhao
    Infectious Diseases Research, Eli Lilly and Company, Drop Code 0438, Indianapolis, Indiana 46285, USA
    Protein Expr Purif 16:331-9. 1999
  5. pmc Identification and characterization of the penicillin-binding protein 2a of Streptococcus pneumoniae and its possible role in resistance to beta-lactam antibiotics
    G Zhao
    Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    Antimicrob Agents Chemother 44:1745-8. 2000
  6. doi request reprint A novel, selective inhibitor of fibroblast growth factor receptors that shows a potent broad spectrum of antitumor activity in several tumor xenograft models
    Genshi Zhao
    Cancer Research, DC0434, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Mol Cancer Ther 10:2200-10. 2011
  7. ncbi request reprint Characterization and development of a peptide substrate-based phosphate transfer assay for the human vascular endothelial growth factor receptor-2 tyrosine kinase
    Genshi Zhao
    Eli Lilly, Lilly Research Laboratories, Cancer Research, Indianapolis, IN 46285, USA
    Anal Biochem 360:196-206. 2007
  8. doi request reprint Identification and characterization of an inhibitor specific to bacterial NAD+-dependent DNA ligases
    Timothy I Meier
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285 0434, USA
    FEBS J 275:5258-71. 2008
  9. pmc Pharmacological inhibition of nicotinamide phosphoribosyltransferase (NAMPT), an enzyme essential for NAD+ biosynthesis, in human cancer cells: metabolic basis and potential clinical implications
    Bo Tan
    Translational Science and Technologies, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    J Biol Chem 288:3500-11. 2013
  10. pmc Acyl carrier protein synthases from gram-negative, gram-positive, and atypical bacterial species: Biochemical and structural properties and physiological implications
    Kelly A McAllister
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    J Bacteriol 188:4737-48. 2006

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Biochemical and molecular analyses of the C-terminal domain of Era GTPase from Streptococcus pneumoniae
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Infectious Diseases Research, Indianapolis, IN 46285 0438, USA
    Microbiology 145:791-800. 1999
    ..coli mutant was also measured. Taken together, these results suggest that the C-terminus of the Era protein might be responsible for the binding of the protein to the cytoplasmic membrane and be essential for function...
  2. pmc Biochemical characterization of penicillin-resistant and -sensitive penicillin-binding protein 2x transpeptidase activities of Streptococcus pneumoniae and mechanistic implications in bacterial resistance to beta-lactam antibiotics
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285 0438, USA
    J Bacteriol 179:4901-8. 1997
    ..We also found that the two purified enzymes did not possess a detectable level of beta-lactam hydrolytic activity. Finally, we show that the expression levels of both PBP2x enzymes were similar during different growth phases...
  3. pmc BOCILLIN FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285 0438, USA
    Antimicrob Agents Chemother 43:1124-8. 1999
    ..Finally, this method also allowed us to compare relative affinities of a PBP2x protein for different beta-lactam antibiotics with the aid of fluorescence polarization technology and to monitor a PBP2x protein during purification...
  4. ncbi request reprint Penicillin-binding protein 2a of Streptococcus pneumoniae: expression in Escherichia coli and purification and refolding of inclusion bodies into a soluble and enzymatically active enzyme
    G Zhao
    Infectious Diseases Research, Eli Lilly and Company, Drop Code 0438, Indianapolis, Indiana 46285, USA
    Protein Expr Purif 16:331-9. 1999
    ..In conclusion, using this expression system, and the purification and refolding methods, large amounts of the soluble GST-PBP2a* protein were obtained and shown to be enzymatically active...
  5. pmc Identification and characterization of the penicillin-binding protein 2a of Streptococcus pneumoniae and its possible role in resistance to beta-lactam antibiotics
    G Zhao
    Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    Antimicrob Agents Chemother 44:1745-8. 2000
    ..When compared with other S. pneumoniae PBPs, PBP 2a exhibited lower affinities for beta-lactam antibiotics, especially penicillins. Therefore, PBP 2a is a low-affinity PBP for beta-lactam antibiotics in S. pneumoniae...
  6. doi request reprint A novel, selective inhibitor of fibroblast growth factor receptors that shows a potent broad spectrum of antitumor activity in several tumor xenograft models
    Genshi Zhao
    Cancer Research, DC0434, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Mol Cancer Ther 10:2200-10. 2011
    ..Furthermore, LY2874455 did not show VEGF receptor 2-mediated toxicities such as hypertension at efficacious doses. Currently, this molecule is being evaluated for its potential use in the clinic...
  7. ncbi request reprint Characterization and development of a peptide substrate-based phosphate transfer assay for the human vascular endothelial growth factor receptor-2 tyrosine kinase
    Genshi Zhao
    Eli Lilly, Lilly Research Laboratories, Cancer Research, Indianapolis, IN 46285, USA
    Anal Biochem 360:196-206. 2007
    ..This approach of designing peptides corresponding to the autophosphorylation sites of Kdr as substrates for the enzyme has general practical implications to other kinases...
  8. doi request reprint Identification and characterization of an inhibitor specific to bacterial NAD+-dependent DNA ligases
    Timothy I Meier
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285 0434, USA
    FEBS J 275:5258-71. 2008
    ....
  9. pmc Pharmacological inhibition of nicotinamide phosphoribosyltransferase (NAMPT), an enzyme essential for NAD+ biosynthesis, in human cancer cells: metabolic basis and potential clinical implications
    Bo Tan
    Translational Science and Technologies, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    J Biol Chem 288:3500-11. 2013
    ..These studies also suggest that altered metabolite levels in tumors can be used as robust pharmacodynamic markers for evaluating NAMPT inhibitors in the clinic...
  10. pmc Acyl carrier protein synthases from gram-negative, gram-positive, and atypical bacterial species: Biochemical and structural properties and physiological implications
    Kelly A McAllister
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    J Bacteriol 188:4737-48. 2006
    ..These findings suggest that AcpS from different microorganisms plays a different role in cellular physiology...
  11. pmc Inhibition of Nicotinamide Phosphoribosyltransferase (NAMPT), an Enzyme Essential for NAD+ Biosynthesis, Leads to Altered Carbohydrate Metabolism in Cancer Cells
    Bo Tan
    Discovery Chemistry, Lilly Research Laboratories, Eli Lilly and Co, Indianapolis, Indiana 46285
    J Biol Chem 290:15812-24. 2015
    ..The potential clinical implications of these findings are also discussed. ..
  12. doi request reprint Derivatization of the tricarboxylic acid intermediates with O-benzylhydroxylamine for liquid chromatography-tandem mass spectrometry detection
    Bo Tan
    Tailored Therapeutics, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285, USA Electronic address
    Anal Biochem 465:134-47. 2014
    ..This method is rapid, sensitive, and reproducible, and it can be used to assess metabolic changes in cancer cells and tumor samples. ..
  13. ncbi request reprint Development of a fluorescence resonance energy transfer assay for measuring the activity of Streptococcus pneumoniae DNA ligase, an enzyme essential for DNA replication, repair, and recombination
    Xinyi Cynthia Chen
    Lilly Research Laboratories, Infectious Diseases Research Drop Code 0428, Lilly Corporate Center, Eli Lilly and Company, Indianapolis, IN 46285 0438, USA
    Anal Biochem 309:232-40. 2002
    ....
  14. pmc Metabolomics analysis of metabolic effects of nicotinamide phosphoribosyltransferase (NAMPT) inhibition on human cancer cells
    Vladimir Tolstikov
    Discovery Chemistry and Technologies, Lilly Research Laboratories, Eli Lilly and Company, 893 S Delaware St, Indianapolis, IN 46285, United States of America
    PLoS ONE 9:e114019. 2014
    ..This study suggests that global metabolomics can be a useful tool in pharmacological studies of the mechanism of action of drugs at a cellular level. ..
  15. ncbi request reprint Dicer is required for embryonic angiogenesis during mouse development
    Wei J Yang
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    J Biol Chem 280:9330-5. 2005
    ....
  16. ncbi request reprint Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase: structural and functional implications
    Julie Q Hang
    Roche Palo Alto, LLC, Palo Alto, CA, USA
    Eur J Biochem 270:4164-72. 2003
    ..Together, these results indicate that the RNA- and membrane-binding domain of Era is a separate, functional entity and does not require the GTPase activity or the GTPase domain of the protein for activity...