Saame Raza Shaikh

Summary

Affiliation: East Carolina University
Country: USA

Publications

  1. pmc A high fat diet containing saturated but not unsaturated fatty acids enhances T cell receptor clustering on the nanoscale
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, East Carolina University, 600 Moye Blvd, Greenville, NC 27834, USA Electronic address
    Prostaglandins Leukot Essent Fatty Acids 100:1-4. 2015
  2. pmc Models of plasma membrane organization can be applied to mitochondrial membranes to target human health and disease with polyunsaturated fatty acids
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, 600 Moye Blvd, Greenville, NC 28590, USA
    Prostaglandins Leukot Essent Fatty Acids 88:21-5. 2013
  3. pmc N-3 fatty acids and membrane microdomains: from model membranes to lymphocyte function
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University 600, Moye Blvd, Greenville, NC 27834, USA
    Prostaglandins Leukot Essent Fatty Acids 87:205-8. 2012
  4. pmc Biophysical and biochemical mechanisms by which dietary N-3 polyunsaturated fatty acids from fish oil disrupt membrane lipid rafts
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA
    J Nutr Biochem 23:101-5. 2012
  5. pmc n-3 Polyunsaturated fatty acids exert immunomodulatory effects on lymphocytes by targeting plasma membrane molecular organization
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology and East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA
    Mol Aspects Med 33:46-54. 2012
  6. doi request reprint Diet-induced docosahexaenoic acid non-raft domains and lymphocyte function
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 28590, USA
    Prostaglandins Leukot Essent Fatty Acids 82:159-64. 2010
  7. doi request reprint Docosahexaenoic acid modifies the clustering and size of lipid rafts and the lateral organization and surface expression of MHC class I of EL4 cells
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    J Nutr 139:1632-9. 2009
  8. pmc Oleic- and docosahexaenoic acid-containing phosphatidylethanolamines differentially phase separate from sphingomyelin
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, 600 Moye Boulevard, Greenville, NC 27834, USA
    Biochim Biophys Acta 1788:2421-6. 2009
  9. pmc Fish oil increases raft size and membrane order of B cells accompanied by differential effects on function
    Benjamin Drew Rockett
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA
    J Lipid Res 53:674-85. 2012
  10. pmc Dendritic cell activation, phagocytosis and CD69 expression on cognate T cells are suppressed by n-3 long-chain polyunsaturated fatty acids
    Heather Teague
    Department of Biochemistry and Molecular Biology, East Carolina University, Greenville, NC, USA
    Immunology 139:386-94. 2013

Collaborators

Detail Information

Publications28

  1. pmc A high fat diet containing saturated but not unsaturated fatty acids enhances T cell receptor clustering on the nanoscale
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, East Carolina University, 600 Moye Blvd, Greenville, NC 27834, USA Electronic address
    Prostaglandins Leukot Essent Fatty Acids 100:1-4. 2015
    ..Furthermore, the effect of the diets was similar in young and middle aged mice. Our data establish proof-of-principle that TCR nanoscale clustering is sensitive to the composition of dietary fat. ..
  2. pmc Models of plasma membrane organization can be applied to mitochondrial membranes to target human health and disease with polyunsaturated fatty acids
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, 600 Moye Blvd, Greenville, NC 28590, USA
    Prostaglandins Leukot Essent Fatty Acids 88:21-5. 2013
    ..Finally, we conclude by postulating future experiments that will augment our understanding of DHA-dependent membrane organization in health and disease...
  3. pmc N-3 fatty acids and membrane microdomains: from model membranes to lymphocyte function
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University 600, Moye Blvd, Greenville, NC 27834, USA
    Prostaglandins Leukot Essent Fatty Acids 87:205-8. 2012
    ..The work raises the intriguing possibility of testing the model at the human level to target health and disease...
  4. pmc Biophysical and biochemical mechanisms by which dietary N-3 polyunsaturated fatty acids from fish oil disrupt membrane lipid rafts
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA
    J Nutr Biochem 23:101-5. 2012
    ..Overall, elucidating the complex mechanisms by which n-3 PUFA acyl chains reorganize membrane architecture will enhance the translation of these fatty acids into the clinic for treating several diseases...
  5. pmc n-3 Polyunsaturated fatty acids exert immunomodulatory effects on lymphocytes by targeting plasma membrane molecular organization
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology and East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA
    Mol Aspects Med 33:46-54. 2012
    ..We conclude by proposing new functional and mechanistic questions in this area of research that will aid in the development of fish oil as adjuvant therapy for treating unresolved chronic inflammation...
  6. doi request reprint Diet-induced docosahexaenoic acid non-raft domains and lymphocyte function
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 28590, USA
    Prostaglandins Leukot Essent Fatty Acids 82:159-64. 2010
    ....
  7. doi request reprint Docosahexaenoic acid modifies the clustering and size of lipid rafts and the lateral organization and surface expression of MHC class I of EL4 cells
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    J Nutr 139:1632-9. 2009
    ..Our study supports the notion that rafts are an important target of DHA's mode of action...
  8. pmc Oleic- and docosahexaenoic acid-containing phosphatidylethanolamines differentially phase separate from sphingomyelin
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, 600 Moye Boulevard, Greenville, NC 27834, USA
    Biochim Biophys Acta 1788:2421-6. 2009
    ....
  9. pmc Fish oil increases raft size and membrane order of B cells accompanied by differential effects on function
    Benjamin Drew Rockett
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA
    J Lipid Res 53:674-85. 2012
    ..Altogether, our studies with B cells support an emerging model that FO increases raft size and membrane order accompanied by functional changes; furthermore, the results highlight differences in EPA and DHA bioactivity...
  10. pmc Dendritic cell activation, phagocytosis and CD69 expression on cognate T cells are suppressed by n-3 long-chain polyunsaturated fatty acids
    Heather Teague
    Department of Biochemistry and Molecular Biology, East Carolina University, Greenville, NC, USA
    Immunology 139:386-94. 2013
    ..The results also show that DC microdomain clustering and microviscosity were not changed by the n-3 LCPUFA intervention used in this study...
  11. pmc Fish oil disrupts MHC class II lateral organization on the B-cell side of the immunological synapse independent of B-T cell adhesion
    Benjamin Drew Rockett
    Department of Biochemistry and Molecular Biology, Brody School of Medicine and the East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834
    J Nutr Biochem 24:1810-6. 2013
    ..Furthermore, the data establish MHC class II lateral organization on the B-cell side of the immunological synapse as a novel molecular target of fish oil. ..
  12. pmc Membrane raft organization is more sensitive to disruption by (n-3) PUFA than nonraft organization in EL4 and B cells
    Benjamin Drew Rockett
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA
    J Nutr 141:1041-8. 2011
    ..The ex vivo data, which confirm previous studies with EL4 cells, provide evidence that (n-3) PUFA consumed through the diet disrupt B cell lipid raft clustering...
  13. pmc DHA-fluorescent probe is sensitive to membrane order and reveals molecular adaptation of DHA in ordered lipid microdomains
    Heather Teague
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, East Carolina University, NC 27834, USA
    J Nutr Biochem 24:188-95. 2013
    ..Mechanistically, this explains how DHA acyl chains can increase order upon formation of lipid microdomains in vivo...
  14. pmc Short-term consumption of n-3 PUFAs increases murine IL-5 levels, but IL-5 is not the mechanistic link between n-3 fatty acids and changes in B-cell populations
    Heather Teague
    Department of Biochemistry and Molecular Biology, East Carolina University East Carolina Diabetes and Obesity Institute, East Carolina University
    J Nutr Biochem 28:30-6. 2016
    ....
  15. pmc Membrane Disordering by Eicosapentaenoic Acid in B Lymphomas Is Reduced by Elongation to Docosapentaenoic Acid as Revealed with Solid-State Nuclear Magnetic Resonance Spectroscopy of Model Membranes
    Mitchell Harris
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University, Greenville, NC and
    J Nutr 146:1283-9. 2016
    ..However, it is not known whether EPA and DHA affect the plasma membrane organization of B lymphomas differently to influence their function...
  16. pmc Aldehyde stress and up-regulation of Nrf2-mediated antioxidant systems accompany functional adaptations in cardiac mitochondria from mice fed n-3 polyunsaturated fatty acids
    Ethan J Anderson
    Department of Pharmacology and Toxicology, East Carolina University, Greenville, NC 27834, USA
    Biochem J 441:359-66. 2012
    ....
  17. pmc n-3 PUFA improves fatty acid composition, prevents palmitate-induced apoptosis, and differentially modifies B cell cytokine secretion in vitro and ex vivo
    Benjamin Drew Rockett
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University, 600 Moye Blvd, Brody 5S 18, Greenville, NC 27834, USA
    J Lipid Res 51:1284-97. 2010
    ..The study highlights that high-fat n-3 PUFA diets can promote pro-inflammatory responses, at least from one cell type...
  18. doi request reprint Mitochondrial inner membrane lipids and proteins as targets for decreasing cardiac ischemia/reperfusion injury
    David A Brown
    Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, USA East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University, Greenville, NC, USA Electronic address
    Pharmacol Ther 140:258-66. 2013
    ..Finally, we point to areas where future study is needed to expand and improve our understanding of mitochondrial pathophysiology during cardiac ischemia/reperfusion. ..
  19. pmc Exercise-induced protection against reperfusion arrhythmia involves stabilization of mitochondrial energetics
    Rick J Alleman
    Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, North Carolina East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University, Greenville, North Carolina and
    Am J Physiol Heart Circ Physiol 310:H1360-70. 2016
    ..The reduction of fatal ventricular arrhythmias through exercise-induced mitochondrial adaptations indicates that mitochondrial therapeutics may be an effective target for the treatment of heart disease. ..
  20. pmc Distinct membrane properties are differentially influenced by cardiolipin content and acyl chain composition in biomimetic membranes
    Edward Ross Pennington
    Department of Biochemistry and Molecular Biology, USA East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University, 115 Heart Drive, Mail Stop 743, Greenville, NC 27834, USA
    Biochim Biophys Acta 1859:257-267. 2017
    ....
  21. doi request reprint N-3 Polyunsaturated Fatty Acids, Lipid Microclusters, and Vitamin E
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, East Carolina University, Greenville, NC, USA Department of Microbiology and Immunology, East Carolina University, Greenville, NC, USA East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC, USA
    Curr Top Membr 75:209-31. 2015
    ..The current evidence suggests that n-3 PUFA acyl chains disrupt lipid raft formation by incorporating primarily into phosphatidylethanolamines but can also incorporate into other lipid species of the lipidome. ..
  22. doi request reprint Increasing mitochondrial membrane phospholipid content lowers the enzymatic activity of electron transport complexes
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834, United States
    Biochemistry 53:5589-91. 2014
    ..These results have strong implications for therapeutic lipid replacement strategies, in which phospholipid modification of the mitochondria is proposed to enhance mitochondrial function. ..
  23. pmc Do fish oil omega-3 fatty acids enhance antioxidant capacity and mitochondrial fatty acid oxidation in human atrial myocardium via PPARĪ³ activation?
    Ethan J Anderson
    1 Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, North Carolina
    Antioxid Redox Signal 21:1156-63. 2014
    ....
  24. pmc n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity
    Heather Teague
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC
    J Lipid Res 54:3130-8. 2013
    ..Altogether, the data show n-3 PUFAs enhance B cell-mediated immunity in vivo, which has implications for immunocompromised populations, such as the obese. ..
  25. pmc N-3 polyunsaturated fatty acids modulate B cell activity in pre-clinical models: Implications for the immune response to infections
    Jarrett Whelan
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, East Carolina Heart Institute, Brody School of Medicine, East Carolina University, Greenville, NC 27834, United States
    Eur J Pharmacol 785:10-7. 2016
    ..Finally, we highlight evidence to show that different n-3 PUFAs are not biologically equivalent, which has implications for the development of future interventions to target B cell activity. ..
  26. pmc Role for phospholipid acyl chains and cholesterol in pulmonary infections and inflammation
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, East Carolina Heart Institute, Brody School of Medicine, East Carolina University ECU, Greenville, North Carolina, USA
    J Leukoc Biol 100:985-997. 2016
    ....
  27. doi request reprint How polyunsaturated fatty acids modify molecular organization in membranes: insight from NMR studies of model systems
    Saame Raza Shaikh
    Department of Biochemistry and Molecular Biology, East Carolina Diabetes and Obesity Institute, East Carolina University, 115 Heart Drive, Room 4117, Mail Stop 743, Greenville, NC 27834, USA
    Biochim Biophys Acta 1848:211-9. 2015
    ..Overall, we highlight the notion that NMR experiments on model membranes suggest a complex model by which n-3 PUFA reorganize lipid microdomains in vivo...
  28. doi request reprint Increasing levels of cardiolipin differentially influence packing of phospholipids found in the mitochondrial inner membrane
    Tonya N Zeczycki
    Department of Biochemistry and Molecular Biology, East Carolina Heart Institute Building, Brody School of Medicine, East Carolina University, 115 Heart Drive, Greenville, NC 27834, United States East Carolina Diabetes and Obesity Institute, East Carolina Heart Institute Building, Brody School of Medicine, East Carolina University, 115 Heart Drive, Greenville, NC 27834, United States
    Biochem Biophys Res Commun 450:366-71. 2014
    ..This has implications for mitochondrial protein activity in response to changing CL levels in microdomains of varying composition. ..