Ganesan Vaidyanathan

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc A kit method for the high level synthesis of [211At]MABG
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Bioorg Med Chem 15:3430-6. 2007
  2. ncbi request reprint Antiepidermal growth factor variant III scFv fragment: effect of radioiodination method on tumor targeting and normal tissue clearance
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 33:101-10. 2006
  3. ncbi request reprint Evaluation of an internalizing monoclonal antibody labeled using N-succinimidyl 3-[131I]iodo-4-phosphonomethylbenzoate ([131I]SIPMB), a negatively charged substituent bearing acylation agent
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, USA
    Nucl Med Biol 31:909-19. 2004
  4. pmc Improved tumor targeting of anti-HER2 nanobody through N-succinimidyl 4-guanidinomethyl-3-iodobenzoate radiolabeling
    Marek Pruszyński
    Department of Radiology, Duke University Medical Center, Durham, North Carolina
    J Nucl Med 55:650-6. 2014
  5. pmc Labeling internalizing anti-epidermal growth factor receptor variant III monoclonal antibody with (177)Lu: in vitro comparison of acyclic and macrocyclic ligands
    Marc Hens
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 36:117-28. 2009
  6. ncbi request reprint Radioiodine and 211At-labeled guanidinomethyl halobenzoyl octreotate conjugates: potential peptide radiotherapeutics for somatostatin receptor-positive cancers
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, USA
    Peptides 25:2087-97. 2004
  7. ncbi request reprint Molecular imaging of alkylguanine-DNA alkyltransferase: further evaluation of radioiodinated derivatives of O6-benzylguanine
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 33:399-407. 2006
  8. pmc Anti-EGFRvIII monoclonal antibody armed with 177Lu: in vivo comparison of macrocyclic and acyclic ligands
    Marc Hens
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 37:741-50. 2010
  9. ncbi request reprint N-succinimidyl 3-[211At]astato-4-guanidinomethylbenzoate: an acylation agent for labeling internalizing antibodies with alpha-particle emitting 211At
    Ganesan Vaidyanathan
    Department of Radiology, Duke University, Durham, North Carolina 27710, USA
    Nucl Med Biol 30:351-9. 2003
  10. ncbi request reprint Catabolism of 4-fluoro-3-iodobenzylguanidine and meta-iodobenzylguanidine by SK-N-SH neuroblastoma cells
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Commun 25:947-55. 2004

Collaborators

Detail Information

Publications41

  1. pmc A kit method for the high level synthesis of [211At]MABG
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Bioorg Med Chem 15:3430-6. 2007
    ..The final doses were sterile and apyrogenic. These results demonstrate that [(211)At]MABG can be prepared via a kit method at radioactivity levels anticipated for initiation of clinical studies...
  2. ncbi request reprint Antiepidermal growth factor variant III scFv fragment: effect of radioiodination method on tumor targeting and normal tissue clearance
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 33:101-10. 2006
    ....
  3. ncbi request reprint Evaluation of an internalizing monoclonal antibody labeled using N-succinimidyl 3-[131I]iodo-4-phosphonomethylbenzoate ([131I]SIPMB), a negatively charged substituent bearing acylation agent
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, USA
    Nucl Med Biol 31:909-19. 2004
    ..8+/-14.2% ID/g; [125I]SGMIB, 41.6+/-15.8% ID/g, at 4 days). These results suggest that [131I]SIPMB may be a viable acylation agent for the radioiodination of internalizing mAbs...
  4. pmc Improved tumor targeting of anti-HER2 nanobody through N-succinimidyl 4-guanidinomethyl-3-iodobenzoate radiolabeling
    Marek Pruszyński
    Department of Radiology, Duke University Medical Center, Durham, North Carolina
    J Nucl Med 55:650-6. 2014
    ..The aim of the present study was to evaluate the tumor-targeting potential of anti-HER2 5F7GGC Nanobody after radioiodination with the residualizing agent N-succinimidyl 4-guanidinomethyl 3-(125/131)I-iodobenzoate (*I-SGMIB)...
  5. pmc Labeling internalizing anti-epidermal growth factor receptor variant III monoclonal antibody with (177)Lu: in vitro comparison of acyclic and macrocyclic ligands
    Marc Hens
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 36:117-28. 2009
    ....
  6. ncbi request reprint Radioiodine and 211At-labeled guanidinomethyl halobenzoyl octreotate conjugates: potential peptide radiotherapeutics for somatostatin receptor-positive cancers
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, USA
    Peptides 25:2087-97. 2004
    ....
  7. ncbi request reprint Molecular imaging of alkylguanine-DNA alkyltransferase: further evaluation of radioiodinated derivatives of O6-benzylguanine
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 33:399-407. 2006
    ..AcO)2-[131I]IBdG, a peracetylated derivative of IBdG, also was investigated as an alternative agent...
  8. pmc Anti-EGFRvIII monoclonal antibody armed with 177Lu: in vivo comparison of macrocyclic and acyclic ligands
    Marc Hens
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 37:741-50. 2010
    ..Because of the short range of its β-emissions, labeling this mAb with (177)Lu would be an attractive approach for the treatment of residual tumor margins remaining after surgical debulking of brain tumors...
  9. ncbi request reprint N-succinimidyl 3-[211At]astato-4-guanidinomethylbenzoate: an acylation agent for labeling internalizing antibodies with alpha-particle emitting 211At
    Ganesan Vaidyanathan
    Department of Radiology, Duke University, Durham, North Carolina 27710, USA
    Nucl Med Biol 30:351-9. 2003
    ..These results suggest that [(211)At]SAGMB may be a useful acylation agent for labeling internalizing mAbs with (211)At...
  10. ncbi request reprint Catabolism of 4-fluoro-3-iodobenzylguanidine and meta-iodobenzylguanidine by SK-N-SH neuroblastoma cells
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Commun 25:947-55. 2004
    ..A fluorine substituted derivative of meta-iodobenzylguanidine (MIBG), 4-fluoro-3-iodobenzylguanidine (FIBG), is retained in SK-N-SH human neuroblastoma cells in vitro to a higher degree than the MIBG...
  11. ncbi request reprint A radioiodinated MIBG-octreotate conjugate exhibiting enhanced uptake and retention in SSTR2-expressing tumor cells
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Bioconjug Chem 18:2122-30. 2007
    ....
  12. pmc Evaluation of an anti-p185(HER2) (scFv-C(H)2-C(H)3)2 fragment following radioiodination using two different residualizing labels: SGMIB and IB-Mal-D-GEEEK
    Ganesan Vaidyanathan
    Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 36:671-80. 2009
    ..The goal of this study was to investigate whether improved tumor targeting could be achieved with this fragment through the use of residualizing radioiodination methods...
  13. pmc Evaluation of anti-podoplanin rat monoclonal antibody NZ-1 for targeting malignant gliomas
    Yukinari Kato
    Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 37:785-94. 2010
    ..The purpose of this study was to determine whether an anti-podoplanin antibody is suitable to target radionuclides to malignant gliomas...
  14. pmc Targeted alpha-particle radiotherapy with 211At-labeled monoclonal antibodies
    Michael R Zalutsky
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 34:779-85. 2007
    ..Nonetheless, two clinical trials have been initiated to date with 211At-labeled mAbs, and others are planned for the near future...
  15. ncbi request reprint Meta-iodobenzylguanidine derivatives containing a second guanidine moiety
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Bioorg Med Chem 12:1649-56. 2004
    ..These results suggest that substitution of guanidine functions, especially a guanidinomethyl group, in MIBG structure may not be advantageous...
  16. ncbi request reprint Nepsilon-(3-[*I]Iodobenzoyl)-Lys5-Nalpha-maleimido-Gly1-GEEEK ([*I]IB-Mal-D-GEEEK): a radioiodinated prosthetic group containing negatively charged D-glutamates for labeling internalizing monoclonal antibodies
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Bioconjug Chem 17:1085-92. 2006
    ..6 +/- 14.3% injected dose per gram versus 17.4 +/- 3.5% ID/g at 72 h) also was observed. These results suggest that [(125)I]IB-Mal-d-GEEEK is a promising reagent for the radioiodination of internalizing mAbs...
  17. ncbi request reprint A 4-methyl-substituted meta-iodobenzylguanidine analogue with prolonged retention in human neuroblastoma cells
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Eur J Nucl Med Mol Imaging 31:1362-70. 2004
    ..The purpose of this study was to evaluate [131I]MeIBG in vitro using the uptake-1 positive SK-N-SH neuroblastoma cell line and in vivo in normal mice and mice bearing human neuroblastoma xenografts...
  18. pmc Preclinical Evaluation of 18F-Labeled Anti-HER2 Nanobody Conjugates for Imaging HER2 Receptor Expression by Immuno-PET
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina
    J Nucl Med 57:967-73. 2016
    ..Herein, we have evaluated the tumor-targeting potential of the 5F7 anti-HER2 Nanobody (single-domain antibody fragment; ∼13 kDa) after (18)F labeling by 2 methods...
  19. pmc N-Succinimidyl guanidinomethyl iodobenzoate protein radiohalogenation agents: influence of isomeric substitution on radiolabeling and target cell residualization
    Jaeyeon Choi
    Department of Radiology, Duke University Medical Center, Durham, NC, USA
    Nucl Med Biol 41:802-12. 2014
    ..To overcome this, we developed the isomeric compound, N-succinimidyl 3-guanidinomethyl-5-[(131)I]iodobenzoate (iso-[(131)I]SGMIB) wherein this bulky group was moved from ortho to meta position...
  20. ncbi request reprint Synthesis and preliminary evaluation of n.c.a. iodoquine: a novel radiotracer with high uptake in cells with high ALDH1 expression
    Bennett B Chin
    Duke University Medical Center, DUMC, Durham, NC 27710, USA
    Curr Radiopharm 5:47-58. 2012
    ..The purpose of this study is to report the novel synthesis of a chloroquine analogue, n.c.a. iodoquine, and the in vitro and in vivo uptake in cells with high ALDH1 content...
  21. ncbi request reprint Specific and high-level targeting of radiolabeled octreotide analogues to human medulloblastoma xenografts
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Clin Cancer Res 9:1868-76. 2003
    ....
  22. pmc An alternative and expedient synthesis of radioiodinated 4-iodophenylalanine
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Appl Radiat Isot 69:1401-6. 2011
    ....
  23. ncbi request reprint O6-3-[131I]iodobenzylguanine: improved synthesis and further evaluation of a potential agent for imaging of alkylguanine-DNA alkyltransferase
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Bioconjug Chem 15:402-8. 2004
    ..After intratumoral injection of [(131)I]IBG in the same xenograft model, the uptake in tumors that were depleted of AGT by BG treatment (165.8 +/- 27.5% ID/g) was about 60% of that in control mice (272.4 +/- 48.2% ID/g; p < 0.05)...
  24. ncbi request reprint Synthesis of N-succinimidyl 4-guanidinomethyl-3-[*I]iodobenzoate: a radio-iodination agent for labeling internalizing proteins and peptides
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina, USA
    Nat Protoc 2:282-6. 2007
    ..Treatment of [*I]Boc-SGMIB with trifluoroacetic acid delivered the final product. The total time for the synthesis and purification of [*I]Boc-SGMIB and its subsequent de-protection is approximately 140 min...
  25. pmc Synthesis and evaluation of 4-[18F]fluoropropoxy-3-iodobenzylguanidine ([18F]FPOIBG): A novel 18F-labeled analogue of MIBG
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710 Electronic address
    Nucl Med Biol 42:673-84. 2015
    ..The goal of this study was to develop an (18)F-labeled analogue of MIBG that like MIBG itself could be synthesized in a single radiochemical step. Towards this end, we designed 4-fluoropropoxy-3-iodobenzylguanidine (FPOIBG)...
  26. pmc N-Succinimidyl 3-((4-(4-[(18)F]fluorobutyl)-1H-1,2,3-triazol-1-yl)methyl)-5-(guanidinomethyl)benzoate ([(18)F]SFBTMGMB): a residualizing label for (18)F-labeling of internalizing biomolecules
    Ganesan Vaidyanathan
    Department of Radiology and Duke University Medical Center, Durham, North Carolina, USA
    Org Biomol Chem 14:1261-71. 2016
    ..In summary, a novel residualizing prosthetic agent for labeling biomolecules with (18)F has been developed. An anti-HER2 nanobody was labeled using this prosthetic group with retention of affinity and immunoreactivity to HER2. ..
  27. pmc Applications of 211At and 223Ra in targeted alpha-particle radiotherapy
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Curr Radiopharm 4:283-94. 2011
    ..In this review, we will discuss the recent developments taken place in the application of 211At-labeled radiopharmaceuticals and give an overview of the current status of 223Ra for targeted α-particle radiotherapy...
  28. ncbi request reprint Synthesis of N-succinimidyl 4-[18F]fluorobenzoate, an agent for labeling proteins and peptides with 18F
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Protoc 1:1655-61. 2006
    ..18F]SFB remains an optimal reagent for labeling proteins and peptides with 18F because of good conjugation yields and metabolic stability...
  29. ncbi request reprint O6-3-[125I]iodobenzyl-2'-deoxyguanosine ([125I]IBdG): synthesis and evaluation of its usefulness as an agent for quantification of alkylguanine-DNA alkyltransferase (AGT)
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, USA
    Bioorg Med Chem 13:3889-98. 2005
    ..However, to be a practical tracer for quantifying cellular AGT, considerable localization of such derivatives need to occur within the cell nucleus where AGT is present predominantly...
  30. pmc Targeting breast carcinoma with radioiodinated anti-HER2 Nanobody
    Marek Pruszyński
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 40:52-9. 2013
    ..This provided motivation for the generation of HER2-specific 5F7GGC Nb, its radioiodination and evaluation for targeting HER2 expressing tumors...
  31. pmc Radioiodinated O(6)-Benzylguanine derivatives containing an azido function
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, USA
    Nucl Med Biol 38:77-92. 2011
    ....
  32. ncbi request reprint Preparation of N-succinimidyl 3-[*I]iodobenzoate: an agent for the indirect radioiodination of proteins
    Ganesan Vaidyanathan
    Department of Radiology, Box 3808, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Protoc 1:707-13. 2006
    ..The total time for the synthesis of [*I]SIB from STB is approximately 95 min. Use of [*I]SIB yields radioiodinated proteins that are considerably more stable in vivo than those radioiodinated by the direct electrophilic method...
  33. pmc Targeting aldehyde dehydrogenase: a potential approach for cell labeling
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
    Nucl Med Biol 36:919-29. 2009
    ..Such an agent might be also helpful in identifying tumors that are resistant to cyclophosphomide chemotherapy because ALDH1 is known to be responsible for this resistance...
  34. ncbi request reprint N-succinimidyl 3-[(131)I]iodo-4-phosphonomethylbenzoate ([(131)I]SIPMB), a negatively charged substituent-bearing acylation agent for the radioiodination of peptides and mAbs
    Sriram Shankar
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, North Carolina 27710, USA
    Bioconjug Chem 14:331-41. 2003
    ..These results suggest that SIPMB could be a potent candidate for labeling internalizing mAbs and warrant further study...
  35. ncbi request reprint Synthesis and preliminary evaluation of 5-[18F] fluoroleucine
    Bennett B Chin
    Department of Radiology, Duke University Medical Center, Durham, North Carolina, USA
    Curr Radiopharm . 2016
    ..Amino acid transporters, such as LAT1, are overexpressed in aggressive prostate and breast carcinomas, directly influencing pathways of growth and proliferation...
  36. pmc D-Amino acid peptide residualizing agents bearing N-hydroxysuccinimido- and maleimido-functional groups and their application for trastuzumab radioiodination
    Marek Pruszyński
    Department of Radiology, Duke University Medical Center, Durham, NC, USA
    Nucl Med Biol 42:19-27. 2015
    ..The purpose of the current study was to synthesize and evaluate a new D-amino acid based agent that might avoid these potential problems...
  37. pmc Radiolabeling and in vitro evaluation of (67)Ga-NOTA-modular nanotransporter--a potential Auger electron emitting EGFR-targeted radiotherapeutic
    Eftychia Koumarianou
    Department of Radiology, Duke University Medical Center, Durham, NC, USA
    Nucl Med Biol 41:441-9. 2014
    ..Hence their conjugation to Auger electron emitters, can cause severe cell killing, by nuclear localization. Herein we evaluate the use of MNT as a platform for targeted radiotherapy with (67)Ga...
  38. pmc Optical and radioiodinated tethered Hsp90 inhibitors reveal selective internalization of ectopic Hsp90 in malignant breast tumor cells
    Jared J Barrott
    Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA
    Chem Biol 20:1187-97. 2013
    ..Our findings show that fluor-tethered or radiolabeled inhibitors that target ectopic Hsp90 can be used to detect breast cancer malignancies through noninvasive imaging. ..
  39. ncbi request reprint Improved xenograft targeting of tumor-specific anti-epidermal growth factor receptor variant III antibody labeled using N-succinimidyl 4-guanidinomethyl-3-iodobenzoate
    Ganesan Vaidyanathan
    Department of Radiology, Duke University Medical Center, Box 3808, Durham, North Carolina 27710, USA
    Nucl Med Biol 29:1-11. 2002
    ..35% in all experiments, indicating a low degree of deiodination in vivo. These results suggest that SGMIB may be a useful reagent for the radioiodination of this internalizing anti-EGFRvIII mAb...
  40. pmc Engineered modular recombinant transporters: application of new platform for targeted radiotherapeutic agents to alpha-particle emitting 211 At
    Andrey A Rosenkranz
    Laboratory of Molecular Genetics of Intracellular Transport, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia
    Int J Radiat Oncol Biol Phys 72:193-200. 2008
    ..To generate and evaluate a modular recombinant transporter (MRT) for targeting 211 At to cancer cells overexpressing the epidermal growth factor receptor (EGFR)...
  41. ncbi request reprint An efficient targeted radiotherapy/gene therapy strategy utilising human telomerase promoters and radioastatine and harnessing radiation-mediated bystander effects
    Marie Boyd
    Department of Radiation Oncology and Targeted Therapy, Glasgow University, Cancer Research UK Beatson Laboratories, Glasgow G61 1BD, Scotland, UK
    J Gene Med 6:937-47. 2004
    ..Combining gene therapy with targeted radiotherapy is attractive because the associated cross-fire irradiation of the latter induces biological bystander effects upon neighbouring cells overcoming low gene transfer efficiency...