Stewart M Gray
Affiliation: Cornell University
- Mechanisms of arthropod transmission of plant and animal virusesS M Gray
Plant Protection Research Unit, Agricultural Research Service, U S Department of Agriculture, Ithaca, New York 14853, USA
Microbiol Mol Biol Rev 63:128-48. 1999..The mechanisms of arthropod-virus associations are many and complex, but common themes are beginning to emerge which may allow the development of novel strategies to ultimately control epidemics caused by arthropod-borne viruses...
- Circulative, "nonpropagative" virus transmission: an orchestra of virus-, insect-, and plant-derived instrumentsStewart Gray
Biological Integrated Pest Management Research Unit, USDA, ARS, Ithaca, New York, USA Department of Plant Pathology and Plant Microbe Biology, Cornell University, Ithaca, New York, USA Electronic address
Adv Virus Res 89:141-99. 2014....
- Plant virus proteins involved in natural vector transmissionS M Gray
USDA, Cornell University, Ithaca, NY 14853, USA
Trends Microbiol 4:259-64. 1996..Both mechanisms probably involve conformational changes of virus proteins during their association with the vector...
- Luteovirus-aphid interactionsStewart Gray
USDA, ARS, Department of Plant Pathology, Cornell University, Ithaca, New York 14853, USA
Annu Rev Phytopathol 41:539-66. 2003..Here we review what is known about the genetic, cellular, and molecular mechanisms regulating these complex and specific virus-aphid interactions...
- Transmission of two viruses that cause Barley Yellow Dwarf is controlled by different loci in the aphid, Schizaphis graminumStewart M Gray
USDA, ARS, USA
J Insect Sci 7:1-15. 2007..These results taken together indicate that multiple loci are involved in the circulative transmission, and that the successful transmission of these closely related viruses is regulated by different sets of aphid genes...
- Genomic and proteomic analysis of Schizaphis graminum reveals cyclophilin proteins are involved in the transmission of cereal yellow dwarf virusCecilia Tamborindeguy
USDA ARS, Robert W Holley Center for Agriculture and Health, Department of Plant Pathology and Plant Microbe Biology, Cornell University, Ithaca, New York, USA
PLoS ONE 8:e71620. 2013..Taken together, these data provide evidence that both cyclophilin A and B interact with CYDV-RPV, and these interactions may be important but not sufficient to mediate virus transport from the hindgut lumen into the hemocoel. ..
- Point mutations in the potato leafroll virus major capsid protein alter virion stability and aphid transmissionIgor B Kaplan
USDA ARS, Plant Protection Research Unit, Ithaca, NY 14853, USA
J Gen Virol 88:1821-30. 2007..This and previous work indicates that the CP structural model has value in predicting the structural architecture of the virion...
- Discovery and targeted LC-MS/MS of purified polerovirus reveals differences in the virus-host interactome associated with altered aphid transmissionMichelle Cilia
Robert W Holley Center for Agriculture and Health, United States Department of Agriculture Agricultural Research Service, Ithaca, New York, USA
PLoS ONE 7:e48177. 2012..Importantly, the genes coding for the plant proteins associated with virus may be examined as targets in breeding cereal crops for new modes of virus resistance that disrupt phloem-virus or aphid-virus interactions...
- Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulationKari A Peter
USDA ARS, Biological Integrated Pest Management Research Unit, Ithaca, NY 14853, USA
J Gen Virol 89:2037-45. 2008..The biological properties of the RTP are sensitive to modifications in both the RTD conserved and variable regions...
- Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infectionStacy L DeBlasio
Boyce Thompson Institute for Plant Research, Ithaca, NY, USA
Proteomics 15:2098-112. 2015....
- Coupling genetics and proteomics to identify aphid proteins associated with vector-specific transmission of polerovirus (luteoviridae)Xiaolong Yang
Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA
J Virol 82:291-9. 2008....
- The C terminus of the polerovirus p5 readthrough domain limits virus infection to the phloemKari A Peter
Biological Integrated Pest Management Research Unit, USDA ARS, Ithaca, New York 148531, USA
J Virol 83:5419-29. 2009....
- A surface loop of the potato leafroll virus coat protein is involved in virion assembly, systemic movement, and aphid transmissionLawrence Lee
Department of Plant Pathology, 334 Plant Science, Cornell University, Ithaca, NY 14853, USA
J Virol 79:1207-14. 2005..These results identified amino acid residues in a surface-oriented loop of the coat protein that are critical for virus assembly and stability, systemic infection of plants, and movement of virus through aphid vectors...
- Visualization of Host-Polerovirus Interaction Topologies Using Protein Interaction Reporter TechnologyStacy L DeBlasio
Boyce Thompson Institute for Plant Research, Ithaca, New York, USA USDA Agricultural Research Service, Ithaca, New York, USA
J Virol 90:1973-87. 2015..These results illuminate the functional diversity of the PLRV-host protein interaction network and demonstrate the usefulness of PIR technology for precision mapping of functional host-pathogen protein interaction topologies...
- Insights into the polerovirus-plant interactome revealed by coimmunoprecipitation and mass spectrometryStacy L DeBlasio
1 Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, U S A
Mol Plant Microbe Interact 28:467-81. 2015....
- Evidence of the biochemical basis of host virulence in the greenbug aphid, Schizaphis graminum (Homoptera: Aphididae)Patricia Pinheiro
Department of Entomology, Cornell University, 2130 Comstock Hall, Ithaca, New York 14853 United States
J Proteome Res 13:2094-108. 2014..Finally, we tested whether aphid genetics can be used to further dissect the genetic mechanisms of biotype virulence in aphids. The genetic data showed that sexual reproduction is a source of biotypic variation observed in S. graminum. ..
- Aphid transmission of plant virusesStewart M Gray
USDA, ARS, Department of Plant Pathology, Cornell University, Ithaca, New York, USA
Curr Protoc Microbiol . 2008..These can affect both virus-vector and virus-host interactions. This unit describes some simple and practical methods for conducting virus transmission experiments using sap-sucking insects...
- Positive Darwinian selection at single amino acid sites conferring plant virus resistanceJ R Cavatorta
Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, USA
J Mol Evol 67:551-9. 2008..Our results indicate that although both methods are surprisingly accurate in their identification of resistance sites, HyPhy appears to more accurately identify biologically significant amino acids using our data set...
- Model System-Guided Protein Interaction Mapping for Virus Isolated from Phloem TissueStacy L DeBlasio
Agricultural Research Service, USDA, Ithaca, New York 14853, United States
J Proteome Res 15:4601-4611. 2016..Our results show that model system proteomics experiments are extremely valuable for understanding protein interactions regulating infection in recalcitrant pathogens such as phloem-limited viruses...
- Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray designJohn S Ramsey
Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA
BMC Genomics 8:423. 2007..However, despite the significant damage inflicted by M. persicae in agricultural systems through direct feeding damage and by its ability to transmit plant viruses, limited genomic information is available for this species...
- Evidence for lysine acetylation in the coat protein of a polerovirusMichelle Cilia
USDA Agricultural Research Service, Ithaca, NY 14853, USA Department of Plant Pathology and Plant Microbe Biology, Cornell University, Ithaca, NY 14853, USA Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA
J Gen Virol 95:2321-7. 2014..We hypothesize that selection favours acetylation of K181 in a fraction of coat protein monomers to stabilize the capsid by promoting intermonomer salt bridge formation. ..
- Host-dependent requirement for the Potato leafroll virus 17-kda protein in virus movementLawrence Lee
United States Department of Agriculture Agricultural Research Service, Department of Plant Pathology, Cornell University, Ithaca, NY 14850, USA
Mol Plant Microbe Interact 15:1086-94. 2002....
- Site-directed mutagenesis and generation of chimeric viruses by homologous recombination in yeast to facilitate analysis of plant-virus interactionsDelin Liang
United States Department of Agriculture Agricultural Research Service and Department of Plant Pathology, Cornell University, Ithaca, NY 14850, USA
Mol Plant Microbe Interact 17:571-6. 2004..This approach will facilitate the analysis of plant-virus interactions of in vivo-generated mutants for many plant viruses, especially those not transmissible mechanically to plants...
- Engineering virus resistance using a modified potato geneJason Cavatorta
Department of Plant Breeding, Cornell University, Ithaca, NY, USA
Plant Biotechnol J 9:1014-21. 2011..Furthermore, the general public may be more amenable to the 'intragenic' nature of this approach because the transferred coding region is modified from a gene in the target crop rather than from a distant species...
- Biometrical genetic analysis of luteovirus transmission in the aphid Schizaphis graminumM E Burrows
USDA ARS Plant Protection Research Unit, Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA
Heredity 98:106-13. 2007..graminum, the transmission efficiency of both CYDV-RPV and BYDV-SGV is regulated by a major gene or set of tightly linked genes, and the transmission efficiency of each virus is influenced by a unique set of minor genes...
- Genetics coupled to quantitative intact proteomics links heritable aphid and endosymbiont protein expression to circulative polerovirus transmissionM Cilia
Robert W Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, USA
J Virol 85:2148-66. 2011....
- Vector specificity of barley yellow dwarf virus (BYDV) transmission: identification of potential cellular receptors binding BYDV-MAV in the aphid, Sitobion avenaeC Li
Department of Entomology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Virology 286:125-33. 2001..avenae. The additional aphid proteins binding the MAV4 anti-ID may represent less specific virus-binding proteins facilitating transmission through different aphid tissues...
- Seasonal abundance of aphids (Homoptera: Aphididae) in wheat and their role as barley yellow dwarf virus vectors in the South Carolina coastal plainJ W Chapin
Department of Entomology, Edisto Research and Education Center, Clemson University, Blackville, SC 29817, USA
J Econ Entomol 94:410-21. 2001..was the last species to colonize wheat each season, and the most abundant. Sitobion avenae was responsible for late-season virus transmission and caused direct yield loss by feeding on heads and flag leaves during an outbreak year...
- A Chinese isolate of barley yellow dwarf virus-PAV represents a third distinct species within the PAV serotypeF Liu
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Arch Virol 152:1365-73. 2007..These data suggest that PAV-CN may have undergone a recombination event with GAV and that PAV-CN represents a third distinct species within the PAV serotype of BYDV...