M Kalafatis

Summary

Affiliation: Cleveland State University
Country: USA

Publications

  1. ncbi request reprint The interaction of fragment 1 of prothrombin with the membrane surface is a prerequisite for optimum expression of factor Va cofactor activity within prothrombinase
    Michael A Bukys
    Cleveland State University, Department of Chemistry, OH 44115, USA
    Thromb Haemost 99:511-22. 2008
  2. pmc Cooperative regulation of the activity of factor Xa within prothrombinase by discrete amino acid regions from factor Va heavy chain
    Melissa A Barhoover
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 47:12835-43. 2008
  3. pmc Role of the acidic hirudin-like COOH-terminal amino acid region of factor Va heavy chain in the enhanced function of prothrombinase
    JAMILA HIRBAWI
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 47:7963-74. 2008
  4. pmc Contribution of amino acid region 334-335 from factor Va heavy chain to the catalytic efficiency of prothrombinase
    Melissa A Barhoover
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 47:6840-50. 2008
  5. pmc Amino acid region 1000-1008 of factor V is a dynamic regulator for the emergence of procoagulant activity
    Joesph R Wiencek
    From the Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115
    J Biol Chem 288:37026-38. 2013
  6. pmc Contribution of amino acid region 659-663 of Factor Va heavy chain to the activity of factor Xa within prothrombinase
    JAMILA HIRBAWI
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 49:8520-34. 2010
  7. ncbi request reprint Coagulation factor V: a plethora of anticoagulant molecules
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, Cleveland, OH 44114, USA
    Curr Opin Hematol 12:141-8. 2005
  8. ncbi request reprint Structural requirements for expression of factor Va activity
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, and The Lerner Research Institute, The Cleveland Clinic Foundation, Ohio, USA
    J Biol Chem 278:33550-61. 2003
  9. ncbi request reprint Identification of a binding site for blood coagulation factor Xa on the heavy chain of factor Va. Amino acid residues 323-331 of factor V represent an interactive site for activated factor X
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 41:12715-28. 2002
  10. ncbi request reprint Factor V: Dr. Jeckyll and Mr. Hyde
    M Kalafatis
    Department of Chemistry, Cleveland State University, OH 44115, USA
    Adv Exp Med Biol 489:31-43. 2001

Research Grants

  1. Functional Domains of Coagulation Factor V
    Michael Kalafatis; Fiscal Year: 2007

Collaborators

Detail Information

Publications34

  1. ncbi request reprint The interaction of fragment 1 of prothrombin with the membrane surface is a prerequisite for optimum expression of factor Va cofactor activity within prothrombinase
    Michael A Bukys
    Cleveland State University, Department of Chemistry, OH 44115, USA
    Thromb Haemost 99:511-22. 2008
    ..Altogether, the data demonstrate that membrane-bound fragment 1 is required to promote optimum Fva cofactor activity which in turn is translated by efficient initial cleavage of prothrombin by prothrombinase at Arg(320)...
  2. pmc Cooperative regulation of the activity of factor Xa within prothrombinase by discrete amino acid regions from factor Va heavy chain
    Melissa A Barhoover
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 47:12835-43. 2008
    ....
  3. pmc Role of the acidic hirudin-like COOH-terminal amino acid region of factor Va heavy chain in the enhanced function of prothrombinase
    JAMILA HIRBAWI
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 47:7963-74. 2008
    ....
  4. pmc Contribution of amino acid region 334-335 from factor Va heavy chain to the catalytic efficiency of prothrombinase
    Melissa A Barhoover
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 47:6840-50. 2008
    ..The data demonstrate that amino acid region 334-335 is required for the rearrangement of enzyme and substrate necessary for efficient catalysis of prothrombin by prothrombinase...
  5. pmc Amino acid region 1000-1008 of factor V is a dynamic regulator for the emergence of procoagulant activity
    Joesph R Wiencek
    From the Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115
    J Biol Chem 288:37026-38. 2013
    ....
  6. pmc Contribution of amino acid region 659-663 of Factor Va heavy chain to the activity of factor Xa within prothrombinase
    JAMILA HIRBAWI
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 49:8520-34. 2010
    ....
  7. ncbi request reprint Coagulation factor V: a plethora of anticoagulant molecules
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, Cleveland, OH 44114, USA
    Curr Opin Hematol 12:141-8. 2005
    ..In this review, we summarize the current state of knowledge with respect to the interactions of the factor Va molecule with the various components of prothrombinase...
  8. ncbi request reprint Structural requirements for expression of factor Va activity
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, and The Lerner Research Institute, The Cleveland Clinic Foundation, Ohio, USA
    J Biol Chem 278:33550-61. 2003
    ....
  9. ncbi request reprint Identification of a binding site for blood coagulation factor Xa on the heavy chain of factor Va. Amino acid residues 323-331 of factor V represent an interactive site for activated factor X
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 41:12715-28. 2002
    ..Our data demonstrate that amino acid sequence 323-331 of factor Va heavy chain contains a binding site for factor Xa...
  10. ncbi request reprint Factor V: Dr. Jeckyll and Mr. Hyde
    M Kalafatis
    Department of Chemistry, Cleveland State University, OH 44115, USA
    Adv Exp Med Biol 489:31-43. 2001
    ..Irregularities in the mechanism of inactivation of factor Va by APC, are associated with thrombotic risk, presumably due to sustained prothrombin activation...
  11. ncbi request reprint Identification and partial characterization of factor Va heavy chain kinase from human platelets
    M Kalafatis
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, Vermont 05405 0068, USA
    J Biol Chem 273:8459-66. 1998
    ..These data suggest a critical role for factor Va phosphorylation on the surface of platelets in regulating cofactor activity...
  12. ncbi request reprint Proteolysis of factor V by cathepsin G and elastase indicates that cleavage at Arg1545 optimizes cofactor function by facilitating factor Xa binding
    R M Camire
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, Vermont 05405, USA
    Biochemistry 37:11896-906. 1998
    ..Thus, cleavage at Arg1545 optimizes cofactor function within prothrombinase by facilitating factor Xa binding to membrane-bound FVa...
  13. ncbi request reprint A control switch for prothrombinase: characterization of a hirudin-like pentapeptide from the COOH terminus of factor Va heavy chain that regulates the rate and pathway for prothrombin activation
    Michael A Bukys
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    J Biol Chem 281:39194-204. 2006
    ..Our data demonstrate that pentapeptide DYDYQ has opposing effects on membrane-bound factor Xa for prothrombin cleavage, depending on the incorporation of factor Va in prothrombinase...
  14. ncbi request reprint Human protein S cleavage and inactivation by coagulation factor Xa
    G L Long
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont 05405 0068, USA
    J Biol Chem 273:11521-6. 1998
    ..Cleavage and inactivation of protein S by factor Xa may be an additional way in which factor Xa exerts its procoagulant effect, after the initial stages of clot formation...
  15. ncbi request reprint Increased tissue factor-initiated prothrombin activation as a result of the Arg506 --> Gln mutation in factor VLEIDEN
    C Van 't Veer
    Department of Biochemistry, University of Vermont, Burlington, Vermont 05405 0068, USA
    J Biol Chem 272:20721-9. 1997
    ..Altogether the data predict that TFPI levels in the lower range of normal values are a risk factor for thrombosis when combined with the Arg506 --> Gln mutation in factor VLEIDEN...
  16. ncbi request reprint Peptidomimetic inhibitors for activated protein C: implications for hemophilia management
    S Butenas
    Department of Biochemistry, University of Vermont, Colchester, VT 05446, USA
    J Thromb Haemost 4:2411-6. 2006
    ..Experiments using a synthetic coagulation proteome model showed that the presence of FV Leiden significantly increased thrombin generation in the absence of FVIII or FIX...
  17. ncbi request reprint The mechanism of inactivation of human factor V and human factor Va by activated protein C
    M Kalafatis
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405 0068
    J Biol Chem 269:31869-80. 1994
    ..However, while this cleavage site is exposed on membrane-bound human factor V, cleavage at Arg506 on the heavy chain of factor Va appears necessary for complete exposure of the cleavage site at Arg306...
  18. ncbi request reprint Amino acids Glu323, Tyr324, Glu330, and Val331 of factor Va heavy chain are essential for expression of cofactor activity
    Lisam S Singh
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    J Biol Chem 278:28335-45. 2003
    ..Our data demonstrate that amino acid residues Glu323, Tyr324, Glu330, and Val331 of factor Va heavy chain are critical for expression of factor Va cofactor activity...
  19. ncbi request reprint The contribution of amino acid residues 1508-1515 of factor V to light chain generation
    E Erdogan
    Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA
    J Thromb Haemost 6:118-24. 2008
    ..Factor (F) V is activated by alpha-thrombin following cleavages at Arg(709), Arg(1,018) and Arg(1,545). Amino acid region 1,490-1,520 of FV is essential for procofactor activation...
  20. ncbi request reprint Megakaryocytes endocytose and subsequently modify human factor V in vivo to form the entire pool of a unique platelet-derived cofactor
    W R Gould
    Department of Biochemistry, University of Vermont College of Medicine, Burlington, VT 05405, USA
    J Thromb Haemost 3:450-6. 2005
    ..This process results in the sequestration of active FVa within the platelet compartment, poised for immediate action subsequent to release from platelets at a site of injury...
  21. ncbi request reprint The contribution of amino acid region ASP695-TYR698 of factor V to procofactor activation and factor Va function
    Daniel O Beck
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    J Biol Chem 279:3084-95. 2004
    ..These data provide functional evidence for an essential and productive contribution of factor Va to the activity of prothrombinase...
  22. ncbi request reprint Incorporation of factor Va into prothrombinase is required for coordinated cleavage of prothrombin by factor Xa
    Michael A Bukys
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    J Biol Chem 280:27393-401. 2005
    ..These data indicate that the interaction of factor Xa with the heavy chain of factor Va strongly influences the catalytic activity of the enzyme resulting in increased rates for both prothrombin-activating cleavages...
  23. ncbi request reprint The role of the membrane in the inactivation of factor va by plasmin. Amino acid region 307-348 of factor V plays a critical role in factor Va cofactor function
    M Kalafatis
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    J Biol Chem 276:18614-23. 2001
    ....
  24. ncbi request reprint Carbohydrate moieties on the procofactor factor V, but not the derived cofactor factor Va, regulate its inactivation by activated protein C
    Jay R Silveira
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, Vermont 05405, USA
    Biochemistry 41:1672-80. 2002
    ....
  25. ncbi request reprint Phenotype and genotype expression in pseudohomozygous factor VLEIDEN : the need for phenotype analysis
    M Kalafatis
    Department of Biochemistry, College of Medicine, University of Vermont, Burlington, USA
    Arterioscler Thromb Vasc Biol 19:336-42. 1999
    ....
  26. ncbi request reprint A matrix-assisted laser desorption/ionization time-of-flight based method for screening the 1691G --> A mutation in the factor V gene
    K Hung
    Department of Chemistry, Cleveland State University, Ohio 44115, USA
    Blood Coagul Fibrinolysis 13:117-22. 2002
    ..The genotypes of all samples were correctly identified. This method is accurate, fast, and potentially allows for simultaneous multiplex genotyping of a number of mutation sites associated with thrombophilia and clot formation...
  27. ncbi request reprint The structural integrity of anion binding exosite I of thrombin is required and sufficient for timely cleavage and activation of factor V and factor VIII
    Michael A Bukys
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    J Biol Chem 281:18569-80. 2006
    ....
  28. ncbi request reprint Determination of the disulfide bridges in factor Va heavy chain
    J Xue
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405 0068
    Biochemistry 33:13109-16. 1994
    ..Each A domain contains a 26 residue "alpha loop at positions 139-165, 471-497, and 1684-1710. The A1 and A2 domains each contain 81 amino acid residue "beta" loops at 220-301 and 579-660.(ABSTRACT TRUNCATED AT 250 WORDS)..
  29. ncbi request reprint Completed three-dimensional model of human coagulation factor va. Molecular dynamics simulations and structural analyses
    Tivadar Orban
    Department of Chemistry, Cleveland State University, Ohio 44195, USA
    Biochemistry 44:13082-90. 2005
    ....
  30. ncbi request reprint The regulation of clotting factors
    M Kalafatis
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405 0068, USA
    Crit Rev Eukaryot Gene Expr 7:241-80. 1997
    ..abstract..
  31. ncbi request reprint Isolation and characterization of an antifactor V antibody causing activated protein C resistance from a patient with severe thrombotic manifestations
    Michael Kalafatis
    Department of Chemistry, Cleveland State University, The Cleveland Clinic Foundation, Cleveland, OH 44115, USA
    Blood 99:3985-92. 2002
    ..Our data provide for the first time the demonstration of an antifactor V antibody not related to the presence of antiphospholipid antibodies, which is responsible for thrombotic rather than hemorrhagic symptoms...
  32. ncbi request reprint Identification of an inactivating cleavage site for alpha-thrombin on the heavy chain of factor Va
    Evrim Erdogan
    Department of Chemistry, Cleveland State University, 2351 Euclid Avenue, Science and Research Center SR 370, Cleveland, Ohio 44115, USA
    Thromb Haemost 98:998-1006. 2007
    ..Our data demonstrate that cleavage of FVa at Arg(643) by alpha-thrombin results in a partially inactive cofactor molecule and provides for an activated protein C (APC)-independent anticoagulant effect of alpha-thrombin...
  33. ncbi request reprint Factor V: a combination of Dr Jekyll and Mr Hyde
    Kenneth G Mann
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405, USA
    Blood 101:20-30. 2003
  34. ncbi request reprint Sequencing of full-length cDNA encoding the alpha and beta subunits of human casein kinase II from human platelets and megakaryocytic cells. Expression of the casein kinase IIalpha intronless gene in a megakaryocytic cell line
    Lisam S Singh
    Department of Chemistry, Cleveland State University, Science Building, 2351 Euclid Avenue, Cleveland, OH 44115, USA
    Biochemistry 41:8935-40. 2002
    ..Expression of the intronless gene was only found in cell line MEG-01. Our data demonstrate expression of the CKIIalpha intronless gene in megakaryocytes and platelets...

Research Grants1

  1. Functional Domains of Coagulation Factor V
    Michael Kalafatis; Fiscal Year: 2007
    ..We have established a system to study phospholipids-driven macromolecular complex formation, which may be a model for the generation of complexes that form extra-and intra-cellularly. ..