Alexei S Soares

Summary

Affiliation: Brookhaven National Laboratory
Country: USA

Publications

  1. ncbi request reprint Experimental methods for measuring accurate high-amplitude phases and their importance in isomorphous replacement experiments
    Alexei S Soares
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 61:1521-7. 2005
  2. pmc Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site
    John H Carra
    United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St, Fort Detrick, MD 21702, USA
    BMC Struct Biol 7:72. 2007
  3. pmc Acoustically mounted microcrystals yield high-resolution X-ray structures
    Alexei S Soares
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Biochemistry 50:4399-401. 2011
  4. pmc Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
    Christian G Roessler
    Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Synchrotron Radiat 20:805-8. 2013
  5. doi request reprint High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density
    Ella Teplitsky
    Office of Educational Programs, Brookhaven National Laboratory, Upton, NY 11973 5000, USA Department of Biochemistry and Cell Biology, Stony Brook University, NY 11794 5215, USA
    J Struct Biol 191:49-58. 2015
  6. doi request reprint Acoustic Methods to Monitor Protein Crystallization and to Detect Protein Crystals in Suspensions of Agarose and Lipidic Cubic Phase
    Daniel L Ericson
    Office of Educational Programs, Brookhaven National Laboratory, Upton, NY, USA Department of Biomedical Engineering, University at Buffalo, SUNY, Buffalo, NY, USA
    J Lab Autom 21:107-14. 2016
  7. pmc Solvent minimization induces preferential orientation and crystal clustering in serial micro-crystallography on micro-meshes, in situ plates and on a movable crystal conveyor belt
    Alexei S Soares
    Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Synchrotron Radiat 21:1231-9. 2014
  8. ncbi request reprint Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database
    John M Skinner
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 62:1340-7. 2006
  9. pmc Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography
    Christian G Roessler
    Photon Sciences Division, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Structure 24:631-40. 2016
  10. pmc Macromolecular crystallography beamline X25 at the NSLS
    Annie Heroux
    Photon Sciences Directorate, Brookhaven National Laboratory, PO Box 5000, Upton, NY 11973 5000, USA
    J Synchrotron Radiat 21:627-32. 2014

Collaborators

  • Allen M Orville
  • Marc Allaire
  • Junko Yano
  • Jan Kern
  • S Michael Soltis
  • Joseph Olechno
  • Michael Becker
  • Christian G Roessler
  • Robert M Sweet
  • Annie Heroux
  • Daniel L Ericson
  • Alexander Scalia
  • Ella Teplitsky
  • Richard Ellson
  • John M Skinner
  • Bryan J Foley
  • Babak Andi
  • Rakhi Agarwal
  • Daniel M Copeland
  • Christina M Cuttitta
  • Karan Joshi
  • Richard Stearns
  • Anthony Kuczewski
  • Howard H Robinson
  • Dieter K Schneider
  • Moh d A Salameh
  • Xiaodong Zhao
  • John H Carra
  • Ann H West
  • Howard Robinson
  • Sean McSweeney
  • Heng Song
  • Erik T Yukl
  • Yingssu Tsai
  • Nikita Podobedov
  • Michael C Browne
  • Eunsun Cho
  • Guangye Han
  • Vittal Yachandra
  • Anjali S Rao
  • Silke Nelsen
  • José F R Bachega
  • Pinghua Liu
  • William M McClintock
  • Diling Zhu
  • Richard G Stearns
  • Ruchira Chatterjee
  • Athina Zouni
  • Rosalie Tran
  • Roberto Alonso-Mori
  • Yasmin N Samara
  • Aina E Cohen
  • Xingyu Yin
  • Harry Vlahos
  • Julia Hellmich
  • Brent Eaton
  • Henrik T Lemke
  • Stuart Myers
  • Martin Bommer
  • Aaron S Brewster
  • Monarin Uervirojnangkoorn
  • Lars Majlof
  • Jim Defever
  • Victor L Davidson
  • Ashley M Drozd
  • James M Glownia
  • Matthew Cowan
  • Lucien P Ghislain
  • Yiping Feng
  • Rick Jackimowicz
  • Johan Hattne
  • Mohamed Ibrahim
  • Mary T Bollard
  • Nicholas K Sauter
  • Denise Laspina
  • Nicholas Zeniou
  • Carrie M Wilmot
  • Sammy Datwani
  • Jeffrey D Mullen
  • Olven Campos
  • Matthew L Cowan
  • Stuart F Myers
  • Joseph Dvorak
  • Leon Flaks
  • Grace Shea-McCarthy
  • Michael Skinner
  • Richard Buono
  • Lonny E Berman
  • Steven Lamarra
  • Evette S Radisky

Detail Information

Publications17

  1. ncbi request reprint Experimental methods for measuring accurate high-amplitude phases and their importance in isomorphous replacement experiments
    Alexei S Soares
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 61:1521-7. 2005
    ....
  2. pmc Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site
    John H Carra
    United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St, Fort Detrick, MD 21702, USA
    BMC Struct Biol 7:72. 2007
    ....
  3. pmc Acoustically mounted microcrystals yield high-resolution X-ray structures
    Alexei S Soares
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Biochemistry 50:4399-401. 2011
    ..X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures...
  4. pmc Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
    Christian G Roessler
    Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Synchrotron Radiat 20:805-8. 2013
    ..Future developments and implementation will focus on achieving ejection and translation of single droplets at a rate of over one hundred per second...
  5. doi request reprint High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density
    Ella Teplitsky
    Office of Educational Programs, Brookhaven National Laboratory, Upton, NY 11973 5000, USA Department of Biochemistry and Cell Biology, Stony Brook University, NY 11794 5215, USA
    J Struct Biol 191:49-58. 2015
    ..This technology pushes towards a faster, automated, and more flexible strategy for high throughput screening of chemical libraries (such as fragment libraries) using as little as 2.5nL of each component. ..
  6. doi request reprint Acoustic Methods to Monitor Protein Crystallization and to Detect Protein Crystals in Suspensions of Agarose and Lipidic Cubic Phase
    Daniel L Ericson
    Office of Educational Programs, Brookhaven National Laboratory, Upton, NY, USA Department of Biomedical Engineering, University at Buffalo, SUNY, Buffalo, NY, USA
    J Lab Autom 21:107-14. 2016
    ..In the future, commercially available sound-emitting transducers with a characteristic frequency near 300 MHz should detect and monitor the growth of individual 3 µm crystals. ..
  7. pmc Solvent minimization induces preferential orientation and crystal clustering in serial micro-crystallography on micro-meshes, in situ plates and on a movable crystal conveyor belt
    Alexei S Soares
    Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Synchrotron Radiat 21:1231-9. 2014
    ..High-quality data able to support a realistic molecular replacement solution were readily obtained from both crystal types using all three serial crystallography strategies. ..
  8. ncbi request reprint Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database
    John M Skinner
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 62:1340-7. 2006
    ..This has included construction of a relational database to organize all details of the experiment and incorporation of a robotic specimen changer to provide automation for high-throughput applications...
  9. pmc Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography
    Christian G Roessler
    Photon Sciences Division, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Structure 24:631-40. 2016
    ..We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples. ..
  10. pmc Macromolecular crystallography beamline X25 at the NSLS
    Annie Heroux
    Photon Sciences Directorate, Brookhaven National Laboratory, PO Box 5000, Upton, NY 11973 5000, USA
    J Synchrotron Radiat 21:627-32. 2014
    ..All major components beginning with the radiation source, and continuing along the beamline and its experimental hutch, have changed to produce a state-of-the-art facility for the scientific community...
  11. pmc Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening
    Christina M Cuttitta
    Office of Educational Programs, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Acta Crystallogr D Biol Crystallogr 71:94-103. 2015
    ..This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening. ..
  12. doi request reprint Maintaining Microclimates during Nanoliter Chemical Dispensations Using Custom-Designed Source Plate Lids
    Bryan J Foley
    Office of Educational Programs, Brookhaven National Laboratory, Upton, NY, USA Fairmont State University, Fairmont, WV, USA
    J Lab Autom 21:115-24. 2016
    ..Controlling degradation and evaporation of chemical building blocks exposed to the atmosphere is increasingly useful as the reagent volume is reduced by advances in liquid handling technology, such as acoustic droplet ejection. ..
  13. ncbi request reprint Mail-in crystallography program at Brookhaven National Laboratory's National Synchrotron Light Source
    Howard Robinson
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 62:1336-9. 2006
    ..It also accelerates the innovation of new productivity tools, which benefit users who come to NSLS beamlines as well as those who use the mail-in program...
  14. ncbi request reprint Three-beam interference is a sensitive measure of the efficacy of macromolecular refinement techniques
    Alexei S Soares
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 59:1716-24. 2003
    ..6 degrees. Modeling the discrete disorder of four amino acids accounted for an additional 0.5 degrees improvement and the final individual phase error was 11.6 degrees...
  15. ncbi request reprint Structural basis for accelerated cleavage of bovine pancreatic trypsin inhibitor (BPTI) by human mesotrypsin
    Moh d A Salameh
    Department of Cancer Biology, Mayo Clinic Cancer Center, Jacksonville, Florida 32224, USA
    J Biol Chem 283:4115-23. 2008
    ..Our model predicts that these deleterious interactions accelerate leaving group dissociation and deacylation...
  16. pmc Crystal structure of a complex between the phosphorelay protein YPD1 and the response regulator domain of SLN1 bound to a phosphoryl analog
    Xiaodong Zhao
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA
    J Mol Biol 375:1141-51. 2008
    ..The ground-state structure presented here suggests that phosphoryl transfer will likely proceed through an associative mechanism involving the formation of a pentacoordinate phosphorus intermediate...
  17. ncbi request reprint Crystal structures of the nitrite and nitric oxide complexes of horse heart myoglobin
    Daniel M Copeland
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA
    J Inorg Biochem 100:1413-25. 2006
    ..13 A. This difference in FeNO conformations as a function of preparative method is reproducible, and suggests a role of the distal pocket in hh MbIINO in stabilizing local FeNO conformational minima...