D J DeRosier

Summary

Affiliation: Brandeis University
Country: USA

Publications

  1. ncbi Correction of high-resolution data for curvature of the Ewald sphere
    D J DeRosier
    W M Keck Institute for Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    Ultramicroscopy 81:83-98. 2000
  2. ncbi Bacterial flagellum: visualizing the complete machine in situ
    David Derosier
    Department of Life Sciences, MS029 Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Curr Biol 16:R928-30. 2006
  3. pmc F-actin bundles are derivatives of microvilli: What does this tell us about how bundles might form?
    D J DeRosier
    W M Keck Institute of Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    J Cell Biol 148:1-6. 2000
  4. pmc Rotational symmetry of the C ring and a mechanism for the flagellar rotary motor
    D R Thomas
    W M Keck Institute for Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02254, USA
    Proc Natl Acad Sci U S A 96:10134-9. 1999
  5. pmc Structures of bacterial flagellar motors from two FliF-FliG gene fusion mutants
    D Thomas
    W. M. Keck Institute for Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, and Department of Biology, Brandeis University, Waltham, Massachusetts 02454
    J Bacteriol 183:6404-12. 2001
  6. pmc Localization of the Salmonella typhimurium flagellar switch protein FliG to the cytoplasmic M-ring face of the basal body
    N R Francis
    Department of Biology, Brandeis University, Waltham, MA 02254
    Proc Natl Acad Sci U S A 89:6304-8. 1992
  7. pmc Mass determination and estimation of subunit stoichiometry of the bacterial hook-basal body flagellar complex of Salmonella typhimurium by scanning transmission electron microscopy
    G E Sosinsky
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02254 9110
    Proc Natl Acad Sci U S A 89:4801-5. 1992
  8. pmc Variable symmetry in Salmonella typhimurium flagellar motors
    Howard S Young
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Biophys J 84:571-7. 2003
  9. pmc The three-dimensional structure of the flagellar rotor from a clockwise-locked mutant of Salmonella enterica serovar Typhimurium
    Dennis R Thomas
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    J Bacteriol 188:7039-48. 2006
  10. ncbi Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism
    Fadel A Samatey
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nature 431:1062-8. 2004

Research Grants

  1. STRUCTURAL STUDIES OF CELLULAR ASSEMBLIES OF ACTIN
    David Derosier; Fiscal Year: 2002
  2. STRUCTURAL STUDIES OF BACTERIAL FLAGELLA
    David Derosier; Fiscal Year: 2003

Collaborators

  • G E Sosinsky
  • R M Macnab
  • James Z Chen
  • Dennis R Thomas
  • Noreen R Francis
  • Tanvir R Shaikh
  • Fadel A Samatey
  • Lewis G Tilney
  • Katsumi Imada
  • Keiichi Namba
  • Hideyuki Matsunami
  • Niels Volkmann
  • Howard S Young
  • Catherine Sukow
  • Jeffry B Stock
  • Huilin Li
  • D Thomas
  • D G Morgan
  • D R Thomas
  • Chen Xu
  • Akio Kitao
  • Shigehiro Nagashima
  • Peter M Wolanin
  • Hongyue Dang
  • Susan Lowey
  • Yimin Lai
  • Greta Ouyang
  • Shahid Khan
  • Dorit Hanein
  • Kathleen M Trybus
  • Eva Nogales
  • William V Nicholson
  • Linda A Melanson
  • Kenneth H Downing
  • Mikhail N Levit
  • N R Francis
  • S Yamaguchi
  • V M Irikura

Detail Information

Publications17

  1. ncbi Correction of high-resolution data for curvature of the Ewald sphere
    D J DeRosier
    W M Keck Institute for Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    Ultramicroscopy 81:83-98. 2000
    ..The simplest of the methods is to take images with different amounts of defocus. For helical structures, however, only one image is needed...
  2. ncbi Bacterial flagellum: visualizing the complete machine in situ
    David Derosier
    Department of Life Sciences, MS029 Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Curr Biol 16:R928-30. 2006
    ..Electron tomography of frozen-hydrated bacteria, combined with single particle averaging, has produced stunning images of the intact bacterial flagellum, revealing features of the rotor, stator and export apparatus...
  3. pmc F-actin bundles are derivatives of microvilli: What does this tell us about how bundles might form?
    D J DeRosier
    W M Keck Institute of Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    J Cell Biol 148:1-6. 2000
  4. pmc Rotational symmetry of the C ring and a mechanism for the flagellar rotary motor
    D R Thomas
    W M Keck Institute for Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02254, USA
    Proc Natl Acad Sci U S A 96:10134-9. 1999
    ..Movement results from the swapping of stud-bound levers with M ring-bound levers. The model predicts that both the M and C rings rotate in the same direction but at different speeds...
  5. pmc Structures of bacterial flagellar motors from two FliF-FliG gene fusion mutants
    D Thomas
    W. M. Keck Institute for Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center, and Department of Biology, Brandeis University, Waltham, Massachusetts 02454
    J Bacteriol 183:6404-12. 2001
    ..The C-ring-M-ring interaction appeared to be altered. In basal bodies with the fusion-deletion protein, the C ring was smaller in diameter, and one of its domains occupied space vacated by missing portions of FliF and FliG...
  6. pmc Localization of the Salmonella typhimurium flagellar switch protein FliG to the cytoplasmic M-ring face of the basal body
    N R Francis
    Department of Biology, Brandeis University, Waltham, MA 02254
    Proc Natl Acad Sci U S A 89:6304-8. 1992
    ..We conclude that the natural location for FliG is at the cytoplasmic face of the M ring and that the stoichiometric ratio between FliF and FliG in wild-type cells is probably 1:1...
  7. pmc Mass determination and estimation of subunit stoichiometry of the bacterial hook-basal body flagellar complex of Salmonella typhimurium by scanning transmission electron microscopy
    G E Sosinsky
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02254 9110
    Proc Natl Acad Sci U S A 89:4801-5. 1992
    ..amp; Aizawa, S.-I. (1990) J. Mol. Biol. 212, 377-387] using quantitative gel electrophoresis. The results of Jones et al., together with ours, account for all proteins in the basal body to within approximately 5% (or 200 kDa)...
  8. pmc Variable symmetry in Salmonella typhimurium flagellar motors
    Howard S Young
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Biophys J 84:571-7. 2003
    ..Implications for motor function are discussed...
  9. pmc The three-dimensional structure of the flagellar rotor from a clockwise-locked mutant of Salmonella enterica serovar Typhimurium
    Dennis R Thomas
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    J Bacteriol 188:7039-48. 2006
    ..We suggest that this inner feature arises from a domain of FliG. We present a hypothetical docking in which the C-terminal motor domain of FliG lies in the C ring, where it can interact intimately with FliM...
  10. ncbi Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism
    Fadel A Samatey
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nature 431:1062-8. 2004
    ..The model reveals the intricate molecular interactions and a plausible switching mechanism for the hook to be flexible in bending but rigid against twisting for its universal joint function...
  11. pmc How to make a curved Drosophila bristle using straight actin bundles
    Lewis G Tilney
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:18785-92. 2005
    ....
  12. pmc A partial atomic structure for the flagellar hook of Salmonella typhimurium
    Tanvir R Shaikh
    Dynamic NanoMachine Project, International Cooperative Research Project, Japan Science and Technology Agency, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 102:1023-8. 2005
    ..typhimurium except for a large insertion (20 kDa). According to difference maps and our fitting, this insertion is found on the outer surface of the hook, consistent with our modeling of the hook...
  13. pmc Three-dimensional structure and organization of a receptor/signaling complex
    Noreen R Francis
    Rosenstiel Biomedical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    Proc Natl Acad Sci U S A 101:17480-5. 2004
    ..This structure of the receptor/signaling complex provides an additional basis for understanding the architecture of the large arrays of chemotaxis receptors, CheA, and CheW found at the cell poles in motile bacteria...
  14. pmc Order, disorder, and perturbations in actin-aldolase rafts
    Catherine Sukow
    The Graduate Program in Biophysics, Brandeis University, Waltham, Massachusetts 02454, USA
    Biophys J 85:525-36. 2003
    ..These results provide a measure of the potential for polymorphism in actin assemblies...
  15. pmc Myosin isoforms show unique conformations in the actin-bound state
    Niels Volkmann
    The Burnham Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 100:3227-32. 2003
    ....
  16. ncbi Subunit organization in a soluble complex of tar, CheW, and CheA by electron microscopy
    Noreen R Francis
    W M Keck Institute for Cellular Visualization, Rosenstiel Basic Medical Sciences Research Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    J Biol Chem 277:36755-9. 2002
    ..Coupled with atomic models of the subunits, our results provide insights into the functional architecture by which the receptor regulates CheA kinase activity during bacterial chemotaxis...
  17. ncbi Microtubule structure at 8 A resolution
    Huilin Li
    Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
    Structure 10:1317-28. 2002
    ....

Research Grants2

  1. STRUCTURAL STUDIES OF CELLULAR ASSEMBLIES OF ACTIN
    David Derosier; Fiscal Year: 2002
    ..We will also look at myosin V (actually a construct having 2 IQ domains) in the presence of calcium in order to visualize the changes associated with regulation. ..
  2. STRUCTURAL STUDIES OF BACTERIAL FLAGELLA
    David Derosier; Fiscal Year: 2003
    ..We propose to obtain 3D maps of the propeller apparatus (hook and filament) at about 4 Angstrom units resolution by electron cryomicroscopy. These maps should permit us to produce a chain tracing of the peptides. ..