James Z Chen
Affiliation: Brandeis University
- SIGNATURE: a single-particle selection system for molecular electron microscopyJames Z Chen
Howard Hughes Medical Institute, W M Keck Institute for Cellular Visualization, Department of Biochemistry, Brandeis University, Waltham, MA 22454 9110, USA
J Struct Biol 157:168-73. 2007..The system design emphasizes both functionality and usability. This software has been released to the EM community and has been successfully applied to macromolecular structural analyses...
- A dose-rate effect in single-particle electron microscopyJames Z Chen
Howard Hughes Medical Institute, Rosenstiel Center MS029, Brandeis University, 415 South Street, Waltham, MA 02545 9110, USA
J Struct Biol 161:92-100. 2008..The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution...
- Beam-induced motion of vitrified specimen on holey carbon filmAxel F Brilot
Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, MS029, 415 South Street, Waltham, MA 02454, USA
J Struct Biol 177:630-7. 2012..We show how alignment and averaging of movie frames can be used to restore high-resolution detail in images affected by beam-induced motion...
- Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EMJames Z Chen
Rosenstiel Basic Medical Research Center, Brandeis University, Waltham, MA 02454, USA
Proc Natl Acad Sci U S A 106:10644-8. 2009..The Ca(2+)-stabilized VP7 intratrimer contact region, which presents important neutralizing epitopes, is unaltered upon capsid binding...
- High-resolution electron microscopy of helical specimens: a fresh look at tobacco mosaic virusCarsten Sachse
Howard Hughes Medical Institute, Brandeis University MS029, 415 South Street, Waltham, MA 02454, USA
J Mol Biol 371:812-35. 2007..Overall, the observed differences suggest that the new model represents a different, more stable state of the virus, compared with the earlier published model...
- Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanismFadel A Samatey
Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
Nature 431:1062-8. 2004..The model reveals the intricate molecular interactions and a plausible switching mechanism for the hook to be flexible in bending but rigid against twisting for its universal joint function...