John C H Spence

Summary

Affiliation: Arizona State University
Country: USA

Publications

  1. ncbi request reprint Diffraction and imaging from a beam of laser-aligned proteins: resolution limits
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Acta Crystallogr A 61:237-45. 2005
  2. ncbi request reprint Dynamic inversion by the method of generalized projections
    - Spence
    Lawrence Berkeley Laboratory, MS 7 222, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Acta Crystallogr A 55:112-118. 1999
  3. ncbi request reprint Electron diffraction from laser-aligned beams of large hydrated molecules
    John C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
    J Electron Microsc (Tokyo) 54:163-8. 2005
  4. ncbi request reprint Solving non-periodic structures using direct methods: phasing diffuse scattering
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Acta Crystallogr A 59:255-61. 2003
  5. ncbi request reprint Lensless imaging: a workshop on "new approaches to the phase problem for non-periodic objects."
    - Spence JCH
    Department of Physics, Arizona State University, Tempe 85287, USA
    Ultramicroscopy 90:1-6. 2001
  6. ncbi request reprint Synchrotron soft X-ray and field-emission electron sources: a comparison
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Ultramicroscopy 93:213-22. 2002
  7. doi request reprint Two-wavelength inversion of multiply scattered soft X-ray intensities to charge density
    J C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Acta Crystallogr A 65:28-38. 2009
  8. ncbi request reprint Atomic string holography
    J C Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287, USA
    Phys Rev Lett 86:5510-3. 2001
  9. pmc Coherent convergent-beam time-resolved X-ray diffraction
    John C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Philos Trans R Soc Lond B Biol Sci 369:20130325. 2014
  10. doi request reprint Lawrence Bragg, microdiffraction and X-ray lasers
    J C H Spence
    Department of Physics, Arizona State University, Tempe, 85282, USA
    Acta Crystallogr A 69:25-33. 2013

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Diffraction and imaging from a beam of laser-aligned proteins: resolution limits
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Acta Crystallogr A 61:237-45. 2005
    ..For a typical virus (TMV), a 50 W continuous-wave laser is adequate for subnanometre resolution at room temperature. The dependence of resolution on laser power, temperature, molecular size, shape and dielectric constant is analyzed...
  2. ncbi request reprint Dynamic inversion by the method of generalized projections
    - Spence
    Lawrence Berkeley Laboratory, MS 7 222, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Acta Crystallogr A 55:112-118. 1999
    ..The method may also be useful for the three-beam X-ray diffraction problem...
  3. ncbi request reprint Electron diffraction from laser-aligned beams of large hydrated molecules
    John C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
    J Electron Microsc (Tokyo) 54:163-8. 2005
    ..The conditions determined allow sub-nanometer resolution charge-density maps to be reconstructed from phased diffraction patterns, so that the secondary structure of the proteins can be observed...
  4. ncbi request reprint Solving non-periodic structures using direct methods: phasing diffuse scattering
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Acta Crystallogr A 59:255-61. 2003
    ..The results are relevant to efforts to phase the scattering from many individual macromolecules that cannot be crystallized, and to the scattering from individual inorganic nanoparticles...
  5. ncbi request reprint Lensless imaging: a workshop on "new approaches to the phase problem for non-periodic objects."
    - Spence JCH
    Department of Physics, Arizona State University, Tempe 85287, USA
    Ultramicroscopy 90:1-6. 2001
    ..The results are summarized in this paper and on the web. A second workshop is planned for 2003...
  6. ncbi request reprint Synchrotron soft X-ray and field-emission electron sources: a comparison
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Ultramicroscopy 93:213-22. 2002
    ..Factors which limit these parameters and methods of measurement are reviewed, and the implications for diffraction, imaging and spectroscopic experiments as well as radiation damage are briefly commented on...
  7. doi request reprint Two-wavelength inversion of multiply scattered soft X-ray intensities to charge density
    J C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Acta Crystallogr A 65:28-38. 2009
    ..A discussion of signs in soft X-ray, positron and electron multiple-scattering theory is given...
  8. ncbi request reprint Atomic string holography
    J C Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287, USA
    Phys Rev Lett 86:5510-3. 2001
    ..Uses of the effect for structure determination and atomic-resolution lensless imaging are suggested...
  9. pmc Coherent convergent-beam time-resolved X-ray diffraction
    John C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Philos Trans R Soc Lond B Biol Sci 369:20130325. 2014
    ..Their use in refinement of the phase-sensitive intensity between overlapping orders is suggested. ..
  10. doi request reprint Lawrence Bragg, microdiffraction and X-ray lasers
    J C H Spence
    Department of Physics, Arizona State University, Tempe, 85282, USA
    Acta Crystallogr A 69:25-33. 2013
    ..In this way, using short pulses instead of freezing, the nexus between dose, resolution and crystal size has been broken, opening the way to time-resolved diffraction without damage for a stream of identical particles...
  11. doi request reprint X-ray lasers for structural and dynamic biology
    J C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287, USA
    Rep Prog Phys 75:102601. 2012
    ..Possibilities for time-resolved chemistry using fast WAXS (solution scattering) from mixtures is reviewed, toward the general goal of making molecular movies of biochemical processes...
  12. doi request reprint Phasing of coherent femtosecond X-ray diffraction from size-varying nanocrystals
    John C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287, USA
    Opt Express 19:2866-73. 2011
    ..By using information on the particle-size distribution within the patterns, this digital method succeeds, using all the data, without knowledge of the distribution of particle size or requiring atomic-resolution data...
  13. ncbi request reprint Coherence and sampling requirements for diffractive imaging
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1505, USA
    Ultramicroscopy 101:149-52. 2004
    ..The results are relevant to the design of diffraction cameras for CDI and plans for femotosecond X-ray diffraction from individual proteins...
  14. ncbi request reprint Three-dimensional diffractive imaging for crystalline monolayers with one-dimensional compact support
    J C H Spence
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    J Struct Biol 144:209-18. 2003
    ..The method should greatly reduce the time and labor needed for data acquisition and analysis in cryo-electron microscopy of organic thin crystals by avoiding the need to record images at high tilt angles...
  15. ncbi request reprint Convergent-beam low energy electron diffraction (CBLEED) and the measurement of surface dipole layers
    J C H Spence
    Department of Physics and Astronomy, College of Liberal Arts and Science, Arizona State University, Tempe, AZ 85287 1504, USA
    Microsc Microanal 10:128-33. 2004
    ..We briefly discuss also the application of this method to oxides and the formation of atomic-resolution scanning images in an idealized instrument in which coherent diffracted LEED orders overlap...
  16. pmc Femtosecond protein nanocrystallography-data analysis methods
    Richard A Kirian
    Department of Physics, Arizona State University, Tempe, Arizona 85287 USA
    Opt Express 18:5713-23. 2010
    ..We determine the number of shots needed for a required accuracy in structure factor measurement and resolution, and investigate the convergence of our Monte-Carlo integration method...
  17. ncbi request reprint Signal, noise, and resolution in correlated fluctuations from snapshot small-angle x-ray scattering
    Richard A Kirian
    Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 84:011921. 2011
    ....
  18. doi request reprint Three-dimensional single-particle imaging using angular correlations from X-ray laser data
    Haiguang Liu
    Department of Physics, Arizona State University, Tempe, AZ 85287, USA
    Acta Crystallogr A 69:365-73. 2013
    ..The method is validated with theoretical data calculated from several representative molecules and applied to the reconstruction of nanoparticles from experimental data collected at the Linac Coherent Light Source...
  19. ncbi request reprint Diffractive electron imaging of nanoparticles on a substrate
    Jinsong Wu
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
    Nat Mater 4:912-6. 2005
    ..The method has the advantage that the reconstructed exit-face wavefunction is free of the aberrations of the objective lens normally used in the microscope, whereas resolution is limited only by thermal vibration and noise...
  20. pmc Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals
    Richard A Kirian
    Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
    Acta Crystallogr A 67:131-40. 2011
    ..The implications for improving the efficiency of data collection by allowing the use of very small crystals, for radiation-damage reduction and for time-resolved diffraction studies at room temperature are discussed...
  21. ncbi request reprint Damped and thermal motion of laser-aligned hydrated macromolecule beams for diffraction
    D Starodub
    Department of Physics and Astronomy, Arizona State University, P O Box 871504 Tempe, Arizona 85287 1504, USA
    J Chem Phys 123:244304. 2005
    ....
  22. ncbi request reprint Dose, exposure time and resolution in serial X-ray crystallography
    D Starodub
    Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287 1504, USA
    J Synchrotron Radiat 15:62-73. 2008
    ....
  23. doi request reprint Solving protein nanocrystals by cryo-EM diffraction: multiple scattering artifacts
    Ganesh Subramanian
    Department of Materials Science and Engineering, Arizona State University, Tempe, AZ, USA
    Ultramicroscopy 148:87-93. 2015
    ..Structure-independent tests for single-scattering and new microfluidic methods for growing and sorting nanocrystals by size are reviewed...
  24. pmc Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
    Uwe Weierstall
    Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
    Nat Commun 5:3309. 2014
    ..Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor...
  25. pmc Femtosecond nanocrystallography using X-ray lasers for membrane protein structure determination
    Petra Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Curr Opin Struct Biol 21:509-16. 2011
    ..We briefly review the possibilities for time-resolved crystallography, and the potential for making 'molecular movies' of membrane proteins at work...
  26. ncbi request reprint Ordering energies and occupancies in doped TiAl, and dedicated ALCHEMI instrumentation
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ, 85287 1504, USA
    Microsc Microanal 8:241-6. 2002
    ..The resulting occupancy information can provide essential input parameters for atomistic alloy design algorithms, and can provide entirely new information on interstitial occupancies in minerals, ceramics, semiconductors, and alloys...
  27. doi request reprint Comparison of Mg L23 edges in MgO and Mg(OH)2--importance of medium-range structure
    Nan Jiang
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Ultramicroscopy 109:122-8. 2008
    ..The differences in the near-edge fine structure between Mg(OH)2 and MgO were interpreted as a result of differences in medium-range structure...
  28. ncbi request reprint Experimental and theoretical improvements on understanding of the O K-edge of TeO2
    Nan Jiang
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Ultramicroscopy 106:123-9. 2006
    ..This indicates that screening is not weak in this oxide, as normally assumed, and that neither the ground state nor a full core-hole model is adequate in quantitative reproduction of the O K-edge in the TeO2 system...
  29. pmc Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser
    Christopher Kupitz
    1 Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287 1604, USA 2
    Nature 513:261-5. 2014
    ..This work shows the great potential for time-resolved serial femtosecond crystallography for investigation of catalytic processes in biomolecules. ..
  30. doi request reprint In situ EELS study of dehydration of Al(OH)₃ by electron beam irradiation
    Nan Jiang
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Ultramicroscopy 111:860-4. 2011
    ..The time-dependent O K-edge shows that the pre-edge peak in the O K-edge of Al(OH)₃ is induced by the formation of unpaired O, rather than an intrinsic feature of the OH bond...
  31. ncbi request reprint Phase recovery and lensless imaging by iterative methods in optical, X-ray and electron diffraction
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Philos Transact A Math Phys Eng Sci 360:875-95. 2002
    ..These new interactions can be expected to provide new information, perhaps, for example, in biology, with the advantage of less damage to samples...
  32. ncbi request reprint Kinematic and dynamical CBED for solving thin organic films at low temperature; experimental tests with anthracene
    J S Wu
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Acta Crystallogr A 58:580-9. 2002
    ..The faint peaks due to H-atom positions may be distinguished. Key issues influencing the validity of the method such as the appropriate dimension of the structure-factor matrix, sample thickness and crystal orientation are discussed...
  33. ncbi request reprint Interpretation of Oxygen K pre-edge peak in complex oxides
    Nan Jiang
    Department of Physics and Astronomy, Arizona State University, Tempe AZ 85287 1504, USA
    Ultramicroscopy 106:215-9. 2006
    ..Our time-resolved energy-loss spectroscopy (EELS) results indicate that the pre-edge peak is due to radiation damage, rather than from any intrinsic feature of the material. It is assigned to the pi* peak due to the O-O interaction...
  34. ncbi request reprint Low-dose, low-temperature convergent-beam electron diffraction and multiwavelength analysis of hydrocarbon films by electron diffraction
    Jinsong Wu
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Microsc Microanal 9:428-41. 2003
    ..A new multiwavelength method of retrieving structure factor moduli and thickness from microdiffraction patterns using two-beam theory is demonstrated for tetracontane...
  35. doi request reprint Interpretation of O K-edge EELS in zircon using a structural variation approach
    Nan Jiang
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Ultramicroscopy 110:89-93. 2009
    ..The positions and intensities of several peaks in the O K-edge EELS spectrum are assigned to specific structural parameters. It suggests that the near-edge structures in EELS can be used to measure atomic structure changes...
  36. ncbi request reprint On the consistency of QCBED structure factor measurements for TiO2 (rutile)
    Bin Jiang
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Microsc Microanal 9:457-67. 2003
    ..Our results will be used to understand the role of d-electrons in the chemical bonding of TiO2...
  37. ncbi request reprint Ab initio phasing of X-ray powder diffraction patterns by charge flipping
    Jinsong Wu
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
    Nat Mater 5:647-52. 2006
    ..Space-group and chemical-composition information are not needed as inputs, and can be determined from the result. The method is illustrated using several experimental powder patterns of indifferent quality...
  38. doi request reprint Time-resolved spectra from millivolt EELS data
    Chufeng Li
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Microsc Microanal 20:837-46. 2014
    ....
  39. doi request reprint Valence electron energy-loss spectroscopy study of ZrSiO₄ and ZrO₂
    Nan Jiang
    Department of Physics, Arizona State University, AZ 85287 1504, USA Electronic address
    Ultramicroscopy 134:68-76. 2013
    ..Although similarities in the VEELS exist between ZrSiO4 and m-ZrO2, two major differences are also noticed and explained in terms of composition and structure differences...
  40. ncbi request reprint On the dose-rate threshold of beam damage in TEM
    Nan Jiang
    Department of Physics, Arizona State University, Tempe AZ 85287 1504, USA
    Ultramicroscopy 113:77-82. 2012
    ..The accumulation of damage can only occur when the damage rate is higher than the recovery rate. For surface sputtering process in TEM, the recovery rate is negligible. Therefore, there is no threshold dose rate for surface sputtering...
  41. ncbi request reprint Phasing diffraction data from a stream of hydrated proteins
    Jin Song Wu
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
    J Opt Soc Am A Opt Image Sci Vis 22:1453-9. 2005
    ..Rev. Lett. 92, 198102 (2004)]. We consider also the effect of empty ice balls on the patterns. The algorithm can also be used to align images with different randomly chosen origins, so that the same embedded subunits overlap...
  42. doi request reprint On the measurement of thickness in nanoporous materials by EELS
    Nan Jiang
    Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
    Ultramicroscopy 111:62-5. 2010
    ..In homogeneous nanoporous systems, on the other hand, the log-ratio method is still valid, but the inelastic scattering mean-free-path is no longer equal to that in the same bulk system...
  43. doi request reprint SEM imaging of liquid jets
    Daniel P DePonte
    Department of Physics, Arizona State University, Tempe, AZ 85287, United States
    Micron 40:507-9. 2009
    ..ESEM imaging of liquid jets offers a valuable research tool for the study of aerosol production, combustion processes, ink-jet generation, and many other attributes of micro- and nanojet systems...
  44. ncbi request reprint Observation of long-range compositional fluctuations in glasses: implications for atomic and electronic structure
    Nan Jiang
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
    Micron 39:698-702. 2008
    ..Comparisons with spectra from compositionally equivalent crystals, and multiple-scattering calculations which include core-hole effects are used to analyze the data...
  45. ncbi request reprint Single molecule diffraction
    J C H Spence
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
    Phys Rev Lett 92:198102. 2004
    ..Iterative methods for solving the phase problem are indicated. Comparisons with a related plan for pulsed x-ray diffraction from single proteins in a molecular beam are provided...
  46. doi request reprint Reconstruction from a single diffraction pattern of azimuthally projected electron density of molecules aligned parallel to a single axis
    D K Saldin
    Department of Physics, University of Wisconsin Milwaukee, PO Box 413, Milwaukee, WI 53201, USA
    Acta Crystallogr A 66:32-7. 2010
    ....
  47. ncbi request reprint Three-dimensional coherent x-ray diffraction imaging of a ceramic nanofoam: determination of structural deformation mechanisms
    A Barty
    Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
    Phys Rev Lett 101:055501. 2008
    ....