Mark C Jenkins

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. ncbi request reprint Gel-Bead Delivery of Eimeria oocysts protects chickens against coccidiosis
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Unites States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
    Avian Dis 56:306-9. 2012
  2. doi request reprint Characterization of the Eimeria maxima sporozoite surface protein IMP1
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, ARS, USDA, Beltsville, MD 20705, USA Electronic address
    Vet Parasitol 211:146-52. 2015
  3. doi request reprint Changes in the levels of Cryspovirus during in vitro development of Cryptosporidium parvum
    M C Jenkins
    Building 1040, BARC East, Beltsville, MD, 200705, USA
    Parasitol Res 114:2063-8. 2015
  4. doi request reprint A rapid method for determining salinomycin and monensin sensitivity in Eimeria tenella
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Building 1040, Agricultural Research Service, USDA, Beltsville, MD 20705, United States Electronic address
    Vet Parasitol 206:153-8. 2014
  5. ncbi request reprint Protecting chickens against coccidiosis in floor pens by administering Eimeria oocysts using gel beads or spray vaccination
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, ARS, USDA, Beltsville, MD 20705, USA
    Avian Dis 57:622-6. 2013
  6. doi request reprint Analysis of giardin expression during encystation of Giardia lamblia
    M C Jenkins
    Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705, USA
    J Parasitol 98:1266-70. 2012
  7. doi request reprint Antibodies to the ventral disc protein delta-giardin prevent in vitro binding of Giardia lamblia trophozoites
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705, USA
    J Parasitol 95:895-9. 2009
  8. ncbi request reprint Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV
    M C Jenkins
    Animal Parasitic Diseases Laboratory, ARS, USDA, Beltsville, MD, USA
    Int J Parasitol 38:1051-5. 2008
  9. ncbi request reprint Comparison of Eimeria species distribution and salinomycin resistance in commercial broiler operations utilizing different coccidiosis control strategies
    M Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Avian Dis 54:1002-6. 2010
  10. doi request reprint Co-infection of chickens with Eimeria praecox and Eimeria maxima does not prevent development of immunity to Eimeria maxima
    M Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
    Vet Parasitol 161:320-3. 2009

Detail Information

Publications69

  1. ncbi request reprint Gel-Bead Delivery of Eimeria oocysts protects chickens against coccidiosis
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Unites States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
    Avian Dis 56:306-9. 2012
    ..These findings indicate that incorporation of Eimeria spp. oocysts in gel-beads may represent an effective way to deliver live oocyst vaccines to day-old chicks for preventing subsequent outbreaks of coccidiosis in the field...
  2. doi request reprint Characterization of the Eimeria maxima sporozoite surface protein IMP1
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, ARS, USDA, Beltsville, MD 20705, USA Electronic address
    Vet Parasitol 211:146-52. 2015
    ..The expression of IMP1 during early stages of in vivo development and its location on the sporozoite surface may explain in part the immunoprotective effect of this protein against E. maxima infection...
  3. doi request reprint Changes in the levels of Cryspovirus during in vitro development of Cryptosporidium parvum
    M C Jenkins
    Building 1040, BARC East, Beltsville, MD, 200705, USA
    Parasitol Res 114:2063-8. 2015
    ..These data suggest that during the first 48 h of C. parvum in vitro development, Cryspovirus is released into the media overlying cells but remains at fairly constant levels within infected cells...
  4. doi request reprint A rapid method for determining salinomycin and monensin sensitivity in Eimeria tenella
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Building 1040, Agricultural Research Service, USDA, Beltsville, MD 20705, United States Electronic address
    Vet Parasitol 206:153-8. 2014
    ..tenella DNA at 24h by both qPCR and sqPCR. This in vitro assay represents a significant advance in developing rapid, cost-effective methods for assessing ionophore sensitivity in E. tenella...
  5. ncbi request reprint Protecting chickens against coccidiosis in floor pens by administering Eimeria oocysts using gel beads or spray vaccination
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, ARS, USDA, Beltsville, MD 20705, USA
    Avian Dis 57:622-6. 2013
    ..These findings indicate that immunization efficacy of gel beads and spray vaccination is improved by raising immunized chicks in contact with litter...
  6. doi request reprint Analysis of giardin expression during encystation of Giardia lamblia
    M C Jenkins
    Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705, USA
    J Parasitol 98:1266-70. 2012
    ..These findings represent the first analysis of giardin transcription in separate populations of trophozoites and cysts during encystation and indicate differential regulation of giardin mRNA expression by these developmental stages...
  7. doi request reprint Antibodies to the ventral disc protein delta-giardin prevent in vitro binding of Giardia lamblia trophozoites
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland 20705, USA
    J Parasitol 95:895-9. 2009
    ..Binding of antibodies to delta-giardin may provide a means of inhibiting attachment of G. lamblia trophozoites to the intestinal epithelium and thereby prevent clinical giardiasis...
  8. ncbi request reprint Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV
    M C Jenkins
    Animal Parasitic Diseases Laboratory, ARS, USDA, Beltsville, MD, USA
    Int J Parasitol 38:1051-5. 2008
    ..parvum-B compared with C. parvum-I oocysts. These findings may indicate a role for CPV in fecundity and possibly virulence of C. parvum...
  9. ncbi request reprint Comparison of Eimeria species distribution and salinomycin resistance in commercial broiler operations utilizing different coccidiosis control strategies
    M Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Avian Dis 54:1002-6. 2010
    ..These findings provide additional evidence for the usefulness of live oocyst vaccines to restore ionophore sensitivity in poultry operations that contain an ionophore-resistant population of Eimeria spp. oocysts...
  10. doi request reprint Co-infection of chickens with Eimeria praecox and Eimeria maxima does not prevent development of immunity to Eimeria maxima
    M Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
    Vet Parasitol 161:320-3. 2009
    ..maxima- or dual E. maxima+E. praecox-immunized chickens. These findings indicate that co-infection of chickens with E. maxima and E. praecox does not prevent development of immunity against E. maxima or E. praecox challenge...
  11. doi request reprint Neospora caninum: cloning and expression of a gene coding for cytokine-inducing profilin
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, U S Department of Agriculture, Beltsville, MD 20705, USA
    Exp Parasitol 125:357-62. 2010
    ..caninum...
  12. doi request reprint Gene expression during excystation of Cryptosporidium parvum oocysts
    Mark C Jenkins
    Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Parasitol Res 109:509-13. 2011
    ..Moreover, of the greater than 1,000 clones analyzed, a high percentage (12.3%) of ESTs detected in excysting oocysts were for hypothetical C. parvum proteins (CpHyP), whose functions are presently unknown...
  13. doi request reprint Detection of Cryptosporidium parvum oocysts by dot-blotting using monoclonal antibodies to Cryptosporidium parvum virus 40-kDa capsid protein
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, U S Department of Agriculture, Beltsville, Maryland 20705, USA
    J Parasitol 94:94-8. 2008
    ..parvum oocysts. MAbCPV40-1 was capable of detecting CPV40 antigen in both soluble and total C. parvum oocyst protein extracts, indicating a potential use for detecting this parasite in environmental samples...
  14. doi request reprint Eimeria praecox infection ameliorates effects of Eimeria maxima infection in chickens
    M Jenkins
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, United States
    Vet Parasitol 155:10-4. 2008
    ..Analysis of oocysts excreted by chickens during dual infection showed little effect of E. praecox on E. maxima oocyst production...
  15. doi request reprint Genomic analysis of Eimeria spp. populations in relation to performance levels of broiler chicken farms in Arkansas and North Carolina
    Ryan S Schwarz
    Animal Parasitic Diseases Lab, United States Department of Agriculture, 10300 Baltimore Ave, Beltsville, Maryland 20705, USA
    J Parasitol 95:871-80. 2009
    ..mivati-like, based on homology searches. Our study demonstrated the utility of analyzing multiple genomic loci to assess composition and polymorphisms of Eimeria spp. populations...
  16. doi request reprint Characterization and localization of an Eimeria-specific protein in Eimeria maxima
    Raymond H Fetterer
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, USDA ARS, Beltsville, MD, 20705, USA
    Parasitol Res 112:3401-8. 2013
    ..The results indicate that ESP is associated with the rhoptry of E. maxima and that the protein may have functions in other developmental stages...
  17. ncbi request reprint Characterisation of macrophage migration inhibitory factor from Eimeria species infectious to chickens
    Katarzyna B Miska
    USDA ARS, Animal Parasitic Diseases Laboratory, 10300 Baltimore Ave BARC East, Beltsville, MD 20705, USA
    Mol Biochem Parasitol 151:173-83. 2007
    ..Phylogenetic analysis revealed that apicomplexan MIF sequences form a sister relationship to MIF-like molecules from Arabidopsis thaliana...
  18. doi request reprint Differing susceptibilities of Eimeria acervulina, Eimeria maxima, and Eimeria tenella oocysts to desiccation
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, ARS, USDA, Beltsville, Maryland 20705 Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, ARS, USDA Beltsville, Maryland 20705
    J Parasitol 99:899-902. 2013
    ..Subsequent studies revealed E. maxima oocyst production was ablated only after 5 days incubation. These findings may explain in part the observed prevalence of E. maxima in litter from commercial poultry operations...
  19. ncbi request reprint Neospora caninum antigens defined by antigen-dependent bovine CD4+ T cells
    Wenbin Tuo
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, USA
    J Parasitol 91:564-8. 2005
    ..This 16-kDa protein was absent in other fractions, and it may be a target of a T-cell response in cattle. Further identification of immunogenic proteins of N. caninum may facilitate development of subunit vaccines against neosporosis...
  20. ncbi request reprint Evaluation of vaccination with Neospora caninum protein for prevention of fetal loss associated with experimentally induced neosporosis in sheep
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
    Am J Vet Res 65:1404-8. 2004
    ..To evaluate the immunologic response of a killed tachyzoite vaccine against Neospora caninum and its effectiveness in preventing fetal loss associated with experimentally induced neosporosis in sheep...
  21. ncbi request reprint Canine neosporosis: clinical signs, diagnosis, treatment and isolation of Neospora caninum in mice and cell culture
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705 2350, USA
    Int J Parasitol 28:1293-304. 1998
    ..caninum. Furthermore, N. caninum (isolates NC-4, NC-5) was isolated in mice and in cell cultures inoculated with neural tissues of these two dogs. Serological diagnosis of neosporosis using a variety of tests is discussed...
  22. ncbi request reprint Experimental infection of sheep with Neospora caninum oocysts
    Ryan O'Handley
    Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
    J Parasitol 88:1120-3. 2002
    ..Antibodies were detected by enzyme-linked immunosorbent assay, Neospora agglutination test, or immunoblotting to either native or recombinant N. caninum antigens in sheep inoculated with oocysts...
  23. ncbi request reprint Cross protection studies with Eimeria maxima strains
    Patricia C Allen
    USDA ARS, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Bldg 1040, Rm 103, BARC East, Beltsville, MD 20705, USA
    Parasitol Res 97:179-85. 2005
    ..This study indicates that parasite fecundity is important in providing good immune stimulation, and should be carefully monitored when characterization of the unique immune potentials of Eimeria strains is undertaken...
  24. ncbi request reprint Effect of polyclonal antisera developed against dense granule-associated Neospora caninum proteins on cell invasion and development in vitro by N. caninum tachyzoites
    P C Augustine
    Parasite Biology and Epidemiology Laboratory, U S Department of Agriculture, Beltsville, Maryland 20705, USA
    Parasitology 119:441-5. 1999
    ..Addition of anti-DG sera at the time of inoculation of tachyzoites, or to the cell cultures after the parasites had entered cells, had little effect on either the percentage or rate of their subsequent development...
  25. doi request reprint Macrophage migration inhibitory factor (MIF) of the protozoan parasite Eimeria influences the components of the immune system of its host, the chicken
    Katarzyna B Miska
    Beltsville Agricultural Research Center, USDA ARS, 10300 Baltimore Ave, Beltsville, MD 20705, USA
    Parasitol Res 112:1935-44. 2013
    ..This study shows that parasite MIF is widely expressed and may have potential effects on the immune system of the host...
  26. ncbi request reprint An improved electron microscopic technique for the immunolabeling of Cryptosporidium parvum oocysts
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
    J Parasitol 92:403-5. 2006
    ..The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa...
  27. ncbi request reprint Present and future control of cryptosporidiosis in humans and animals
    Mark C Jenkins
    Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Building 1040, BARC East, Beltsville, MD 20705, USA
    Expert Rev Vaccines 3:669-71. 2004
    ..parvum infection in humans. In the near future, control of this parasitic disease in humans and animals will rely on a combination of passive immunotherapy and selective drug treatment...
  28. ncbi request reprint Specific detection of Neospora caninum oocysts in fecal samples from experimentally-infected dogs using the polymerase chain reaction
    D E Hill
    USDA, ARS, LPSI, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705, USA
    J Parasitol 87:395-8. 2001
    ..The assay should be useful in epidemiological surveys of both domestic and wild canine hosts and in investigations of oocyst biology in experimental infections...
  29. ncbi request reprint Improved polymerase chain reaction technique for determining the species composition of Eimeria in poultry litter
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, ARS, USDA, Beltsville, MD 20705, USA
    Avian Dis 50:632-5. 2006
    ..The internal DNA standards were then used in Eimeria-specific ITS 1 PCR, and they were found to be capable of detecting E. acervulina, E. maxima, E. praecox, and E. tenella oocysts isolated directly from poultry litter...
  30. doi request reprint Immunization of female BALB/c mice with Neospora cyclophilin and/or NcSRS2 elicits specific antibody response and prevents against challenge infection by Neospora caninum
    Wenbin Tuo
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, USDA ARS, Beltsville, MD 20705, USA
    Vaccine 29:2392-9. 2011
    ..06) from that of the mock-vaccinated group. The results of the present study indicate that NcCyP is a highly efficacious vaccine candidate which may be useful in protection against Neospora infection...
  31. ncbi request reprint Characterization and potential use of a Cryptosporidium parvum virus (CPV) antigen for detecting C. parvum oocysts
    K E Kniel
    Animal Parasitic Diseases Laboratory, USDA ARS, Building 1040, Room 100, BARC East, Beltsville, MD 20705, USA
    J Microbiol Methods 58:189-95. 2004
    ..This study indicates that CPV is an excellent target for sensitive detection of C. parvum oocysts in water, but may persist for an indefinite time after oocysts become non-infectious...
  32. ncbi request reprint Vaccination of ewes for prevention of vertical transmission of Neospora caninum
    R M O'Handley
    Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
    Am J Vet Res 64:449-52. 2003
    ..To evaluate the immunologic response of a killed tachyzoite vaccine against Neospora caninum and its effectiveness in preventing vertical transmission of N caninum in sheep...
  33. ncbi request reprint Characterization of the Oregon isolate of Neospora hughesi from a horse
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Maryland 20705 2350, USA
    J Parasitol 87:345-53. 2001
    ..caninum from dogs and cattle indicated that the Oregon isolate of N. hughesi is distinct from N. caninum isolates from cattle and dogs...
  34. doi request reprint A highly divergent 33 kDa Cryptosporidium parvum antigen
    Mark C Jenkins
    Environmental, Microbial, and Food Safety Laboratory, ARS, USDA, Beltsville, Maryland 20705
    J Parasitol 100:527-31. 2014
    ..parvum oocyst excystation revealed transcripts only in excysting sporozoites. Thus, Cp33 represents one of a small number of proteins shown to differentiate C. parvum from C. hominis sporozoites and oocysts...
  35. ncbi request reprint Recovery and detection of Cryptosporidium parvum oocysts from water samples using continuous flow centrifugation
    James A Higgins
    USDA ARS, Rm 202, Bldg 173, 10300 Baltimore Blvd, Beltsville, MD 20705, USA
    Water Res 37:3551-60. 2003
    ..e., US EPA methods 1622/1623), CFC and IMS may provide a more rapid and economical alternative for isolation of C. parvum oocysts from highly turbid water samples containing small quantities of oocysts...
  36. doi request reprint Characterization of the antigen SO7 during development of Eimeria tenella
    R H Fetterer
    Animal Parasitic Diseases Laboratory, Henry A Wallace Beltsville Agricultural Research Center USDA ARS, Beltsville, Maryland 20750, USA
    J Parasitol 93:1107-13. 2007
    ..The results suggest that SO7 is closely associated with the SZ RB and is developmentally regulated but may not play a direct role in cellular invasion...
  37. doi request reprint Metam sodium reduces viability and infectivity of Eimeria oocysts
    R H Fetterer
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Henry A Wallace, Beltsville Agricultural Research Center, Beltsville, Maryland 20705, USA
    J Parasitol 96:632-7. 2010
    ..The results indicate that MS could be used to reduce coccidial contamination of poultry litter...
  38. doi request reprint Identification and characterization of a serpin from Eimeria acervulina
    R H Fetterer
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, U S Department of Agriculture, Henry A Wallace Beltsville Agricultural Research Center, Beltsville, Maryland, USA
    J Parasitol 94:1269-74. 2008
    ..Although the function of the E. acervulina serpin remains unknown the results further suggest that serpin is secreted by the parasite where it may be involved in cell invasion and other basic developmental processes...
  39. ncbi request reprint Oral infection of calves with Neospora caninum oocysts from dogs: humoral and cellular immune responses
    T De Marez
    US Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Immunology and Disease Resistance Laboratory, Beltsville, MD 20705 2350, USA
    Int J Parasitol 29:1647-57. 1999
    ..caninum oocysts collected from dogs, and that the animals were systematically sensitised by parasite antigen...
  40. doi request reprint Molecular characterization and phylogenetic analysis of Eimeria from turkeys and gamebirds: implications for evolutionary relationships in Galliform birds
    K B Miska
    USDA ARS, Animal Parasitic Diseases Laboratory, 10300 Baltimore Ave, Bldg 1042 BARC East, Beltsville, Maryland 20705, USA
    J Parasitol 96:982-6. 2010
    ..tenella and E. necatrix that infect the ceca, while the other includes Eimeria acervulina, Eimeria brunetti, Eimeria maxima, and Eimeria mitis, which infect the upper regions of the intestine...
  41. doi request reprint Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer (Odocoileus virginianus) from Iowa and Minnesota using four serologic tests
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 161:330-4. 2009
    ..gondii and N. caninum were detected in 47 deer. Very high (84.9%) seropositivity of N. caninum in fawns suggests high rate of congenital transmission of the parasite. Seropositivity in each test at different titers is discussed...
  42. doi request reprint Detection and comparison of Giardia virus (GLV) from different assemblages of Giardia duodenalis
    K B Miska
    USDA ARS, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705, USA
    J Parasitol 95:1197-200. 2009
    ..Because many of the specimens that contained cysts were found to be negative for GLV, it appears that this test for capsid protein is of limited value for the purposes of detecting G. lamblia...
  43. ncbi request reprint Effect of conditioned media from chicken and turkey intestinal cell cultures on invasion by sporozoites of three species of avian coccidia
    P C Augustine
    United States Department of Agriculture, Livestock and Poultry Sciences Institute, Beltsville, Maryland 20705, USA
    J Eukaryot Microbiol 45:344-6. 1998
    ..tenella, the chicken cecal coccidium. Although morphologically dissimilar when they were first plated, the gross appearance and growth of the turkey and chicken cells when conditioned media was collected was comparable...
  44. ncbi request reprint Temporal variability of Cryptosporidium in the Chesapeake Bay
    R Fayer
    Animal Waste Pathogen Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Parasitol Res 88:998-1003. 2002
    ..In 28% of 53 collections, C. parvum genotypes 1 and 2 and C. baileyi were identified by PCR and gene sequencing. Oocyst infectivity was confirmed from 37.5% of 40 collections by initiating C. parvum genotype 2 infections in mice...
  45. ncbi request reprint Redescription of Neospora caninum and its differentiation from related coccidia
    J P Dubey
    Parasite Biology Laboratory, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, United States Department of Agriculture, Building 1001, BARC East, Beltsville, MD 20705 2350, USA
    Int J Parasitol 32:929-46. 2002
    ..In this article, the authors redescribe the parasite, distinguish it from related coccidia, and provide accession numbers to its type specimens deposited in museums...
  46. ncbi request reprint Real-time PCR for the detection of Cryptosporidium parvum
    J A Higgins
    USDA ARS, Rm 202, Bldg 173, 10300 Baltimore Blvd, Beltsville, MD 20705, USA
    J Microbiol Methods 47:323-37. 2001
    ..For samples with few oocysts and high concentrations of PCR inhibitor(s), such as manure, nested PCR is necessary for detection...
  47. ncbi request reprint Advances and prospects for subunit vaccines against protozoa of veterinary importance
    M C Jenkins
    Immunology and Disease Resistance Laboratory, Agricultural Research Service, US Department of Agriculture USDA, Beltsville, MA 20705, USA
    Vet Parasitol 101:291-310. 2001
    ....
  48. pmc Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR
    J A Higgins
    U S Department of Agriculture Agricultural Research Service, Beltsville, Maryland 20705, USA
    Appl Environ Microbiol 67:5321-4. 2001
    ..All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum...
  49. ncbi request reprint Serological investigation of an outbreak of Neospora caninum-associated abortion in a dairy herd in southeastern United States
    M C Jenkins
    US Department of Agriculture, ARS LPSI, Immunology and Disease Resistance Laboratory, Beltsville, MD 20705, USA
    Vet Parasitol 94:17-26. 2000
    ..caninum infection. Of the cows at risk for abortion, the mean serological AI of aborting cows was significantly lower (P<0.05) than mean serological AI of non-aborting cows...
  50. ncbi request reprint A competitive PCR assay for quantitative detection of Neospora caninum
    S Liddell
    US Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Laboratory, Beltsville, MD 20705, USA
    Int J Parasitol 29:1583-7. 1999
    ..Incorporation of the competitor molecule in the detection assay also provides a control for PCR failure and facilitates identification of truly negative samples...
  51. pmc Cloning and expression of a DNA sequence encoding a 41-kilodalton Cryptosporidium parvum oocyst wall protein
    M C Jenkins
    Immunology and Disease Resistance Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U S Department of Agriculture, Beltsville, Maryland 20705, USA
    Clin Diagn Lab Immunol 6:912-20. 1999
    ..parvum infections. These results indicate that recombinant CP41 antigen may have potential in the immunodiagnosis of cryptosporidiosis...
  52. pmc Cryptosporidium parvum in oysters from commercial harvesting sites in the Chesapeake Bay
    R Fayer
    U S Department of Agriculture, Beltsville, Maryland 20705, USA
    Emerg Infect Dis 5:706-10. 1999
    ..We identified oocysts of C. parvum in oysters from seven sites in the Chesapeake Bay area. These findings document the presence of C. parvum infectious for humans in oysters intended for human consumption...
  53. ncbi request reprint Biologic, morphologic, and molecular characterisation of Neospora caninum isolates from littermate dogs
    J P Dubey
    US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East, Building 1040, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    Int J Parasitol 34:1157-67. 2004
    ..caninum tissue cysts from the brain of one dog. Tissue cysts were infective orally to mice and gerbils, but not to cats and dogs. The isolates were also identified as N. caninum by PCR and sequence analysis...
  54. ncbi request reprint A conserved 19-kDa Eimeria tenella antigen is a profilin-like protein
    R H Fetterer
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Henry A Wallace Beltsville Agricultural Research Center, Beltsville, Maryland 20705, USA
    J Parasitol 90:1321-8. 2004
    ..SZ-1 also partially inhibited the polymerization of actin. The current results are consistent with the classification of SZ-1 as a profilin-related protein...
  55. ncbi request reprint Neosporosis in Beagle dogs: clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninum
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agricultural, Building 1001, Beltsville, MD 20705, USA
    Vet Parasitol 149:158-66. 2007
    ..caninum antibody titers were still persistent in these pups at 23 months of age. Results indicate that medication with Clindamycin can improve clinical condition but not eliminate N. caninum infection...
  56. ncbi request reprint A rapid method for producing highly purified Cryptosporidium parvum oocysts
    C N O'Brien
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA, Beltsville Agricultural Research Center, Beltsville, Maryland 20705, USA
    J Parasitol 93:434-6. 2007
    ..parvum oocysts in NaCl, followed by ether extraction to solubilize lipids in calf feces. This procedure regularly yields > 10(9) purified C. parvum oocysts within 1-2 days of feces collection...
  57. ncbi request reprint Hyperimmune bovine colostrum specific for recombinant Cryptosporidium parvum antigen confers partial protection against cryptosporidiosis in immunosuppressed adult mice
    M C Jenkins
    Immunology and Disease Resistance Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Vaccine 17:2453-60. 1999
    ..parvum infection...
  58. ncbi request reprint Neospora caninum detected in feral rodents
    M C Jenkins
    United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Building 1040, BARC East, Beltsville, MD 20705, USA
    Vet Parasitol 143:161-5. 2007
    ..Although mice and rats had N. caninum by PCR testing, clinical signs of N. caninum infection were not observed nor were N. caninum parasites observed in gerbils or KO mice inoculated with the rodent brain tissue...
  59. ncbi request reprint The relationship of Hammondia hammondi and Sarcocystis mucosa to other heteroxenous cyst-forming coccidia as inferred by phylogenetic analysis of the 18S SSU ribosomal DNA sequence
    M C Jenkins
    Parasite Biology and Epidemiology Laboratory, USDA, BARC East, Beltsville, MD 20705, USA
    Parasitology 119:135-42. 1999
    ..caninum and B. besnoiti. The weight of evidence shows that S. mucosa, S. neurona, and Frenkelia species form a clade thereby supporting the conclusion that Sarcocystis is paraphyletic...
  60. ncbi request reprint HPLC purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Vet Parasitol 131:227-34. 2005
    ..coli proteins improves the performance of recombinant NcGRA6 ELISA in diagnosing bovine neosporosis, and may have applicability to the use of recombinant proteins in diagnosing other infectious agents...
  61. ncbi request reprint Improving the specificity of 16S rDNA-based polymerase chain reaction for detecting Borrelia burgdorferi sensu lato-causative agents of human Lyme disease
    T L Cyr
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, USDA ARS, Building 1040, Room 100, BARC East, Beltsville, MD 20705, USA
    J Appl Microbiol 98:962-70. 2005
    ....
  62. ncbi request reprint Neospora caninum and Hammondia heydorni are separate species/organisms
    J P Dubey
    Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agriculture Research Service, United States Dept of Agriculture, Building 1001, Beltsville, MD 20705 2350, USA
    Trends Parasitol 18:66-9. 2002
    ..It was recently proposed that they are one species. In this paper, we review the biology and morphology of these parasites and present evidence that N. caninum and H. heydorni are separate species...
  63. ncbi request reprint Application of polymerase chain reaction based on ITS1 rDNA to speciate Eimeria
    M C Jenkins
    Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Avian Dis 50:110-4. 2006
    ..The described method represents a rapid means for determining the major Eimeria species in a poultry operation and may be helpful in choosing a particular live oocyst vaccine formulation to protect chickens against coccidiosis...
  64. ncbi request reprint Observations on the gross pathology of Eimeria praecox infections in chickens
    P C Allen
    Animal Parasitic Diseases Laboratory, Agricultural Research Service, U S Department of Agriculture, Building 1040, Room 103, BARC East, Beltsville, MD 20705, USA
    Avian Dis 54:834-40. 2010
    ..praecox with other Eimeria species, especially in drug sensitivity testing of Eimeria oocysts recovered from litter and in the evaluation of live oocyst vaccines...
  65. doi request reprint Expression of nutrient transporters in duodenum, jejunum, and ileum of Eimeria maxima-infected broiler chickens
    Raymond H Fetterer
    Animal Parasitic Diseases Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Beltsville, MD, 20705, USA
    Parasitol Res 113:3891-4. 2014
    ..maxima alters the expression of AAT and LEAP2 throughout the small intestine...
  66. ncbi request reprint Effects of gamma radiation on viability of Encephalitozoon spores
    X Li
    Animal Waste Pathogen Laboratory, Agricultural Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, Beltsville, Maryland 20705 2350, USA
    J Parasitol 88:812-3. 2002
    ..0 kGy, complete inhibition was not obtained. These findings provide a baseline for investigating the dose levels required to render food products safe when kept under varying temperature, moisture, and other storage conditions...
  67. pmc A recombinant attenuated Salmonella enterica serovar Typhimurium vaccine encoding Eimeria acervulina antigen offers protection against E. acervulina challenge
    Vjollca Konjufca
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Infect Immun 74:6785-96. 2006
    ....
  68. ncbi request reprint Neospora caninum-like oocysts observed in feces of free-ranging red foxes (Vulpes vulpes) and coyotes (Canis latrans)
    Wendela Wapenaar
    Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, C1A 4P3, Prince Edward Island, Canada
    J Parasitol 92:1270-4. 2006
    ..caninum. PCR using primers specific for Hammondia heydorni failed to yield an amplification product from these DNA samples...
  69. ncbi request reprint High hydrostatic pressure and UV light treatment of produce contaminated with Eimeria acervulina as a Cyclospora cayetanensis surrogate
    Kalmia E Kniel
    Department of Animal and Food Sciences, University of Delaware, 531 South College Avenue, Townsend Hall, Newark, Delaware 19716, USA
    J Food Prot 70:2837-42. 2007
    ..Both treatments yielded healthy animals; however, HPP was more effective, as indicated by results for produce with higher contamination levels...