Pina M Fratamico

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. pmc Prevalence and characterization of shiga toxin-producing Escherichia coli in swine feces recovered in the National Animal Health Monitoring System's Swine 2000 study
    Pina M Fratamico
    Microbial Food Safety Research Unit, Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, PA 19038, USA
    Appl Environ Microbiol 70:7173-8. 2004
  2. ncbi request reprint DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, PA 19038, USA
    Can J Microbiol 51:515-22. 2005
  3. pmc Sequence of the Escherichia coli O121 O-antigen gene cluster and detection of enterohemorrhagic E. coli O121 by PCR amplification of the wzx and wzy genes
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    J Clin Microbiol 41:3379-83. 2003
  4. doi request reprint Influence of primer sequences and DNA extraction method on detection of non-O157 Shiga toxin-producing Escherichia coli in ground beef by real-time PCR targeting the eae, stx, and serogroup-specific genes
    Jamie L Wasilenko
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Prot 75:1939-50. 2012
  5. doi request reprint Characterization of Shiga toxin-producing Escherichia coli strains isolated from swine feces
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    Foodborne Pathog Dis 5:827-38. 2008
  6. doi request reprint Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
    Foodborne Pathog Dis 8:601-7. 2011
  7. ncbi request reprint Testing of swine feces obtained through the National Animal Health Monitoring System's Swine 2000 study for the presence of Escherichia coli O157:H7
    Ingrid Feder
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 70:1489-92. 2007
  8. doi request reprint Application of ozonated dry ice (ALIGAL™ Blue Ice) for packaging and transport in the food industry
    Pina M Fratamico
    U S Dept of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Sci 77:M285-91. 2012
  9. doi request reprint DNA sequence and analysis of a 90.1-kb plasmid in Shiga toxin-producing Escherichia coli (STEC) O145:NM 83-75
    Xianghe Yan
    Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E Mermaid Lane, Wyndmoor, PA 19038, USA
    Plasmid 68:25-32. 2012
  10. doi request reprint Latex agglutination assays for detection of non-O157 Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145
    Marjorie B Medina
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Prot 75:819-26. 2012

Collaborators

Detail Information

Publications45

  1. pmc Prevalence and characterization of shiga toxin-producing Escherichia coli in swine feces recovered in the National Animal Health Monitoring System's Swine 2000 study
    Pina M Fratamico
    Microbial Food Safety Research Unit, Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, PA 19038, USA
    Appl Environ Microbiol 70:7173-8. 2004
    ..It is noteworthy that no isolates of serogroup O157 were recovered. Results of this study indicate that swine in the United States harbor STEC that can potentially cause human illness...
  2. ncbi request reprint DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, PA 19038, USA
    Can J Microbiol 51:515-22. 2005
    ..coli O103 from non-toxigenic strains. The PCR assays can be employed to identify E. coli serogroup O103, replacing antigen-based serotyping, and to potentially detect the organism in food, fecal, or environmental samples...
  3. pmc Sequence of the Escherichia coli O121 O-antigen gene cluster and detection of enterohemorrhagic E. coli O121 by PCR amplification of the wzx and wzy genes
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    J Clin Microbiol 41:3379-83. 2003
    ..coli O121 showed specificity for E. coli O121. Thus, the PCR assay can be employed to reliably identify E. coli O121 and to potentially detect the organism in food, fecal, and environmental samples...
  4. doi request reprint Influence of primer sequences and DNA extraction method on detection of non-O157 Shiga toxin-producing Escherichia coli in ground beef by real-time PCR targeting the eae, stx, and serogroup-specific genes
    Jamie L Wasilenko
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Prot 75:1939-50. 2012
    ..The use of different stx primers and probes increased the subtypes detected to include stx(1d), stx(2e), and stx(2g), and sequence data showed that modification of the eae primer should allow the known eae subtypes to be detected...
  5. doi request reprint Characterization of Shiga toxin-producing Escherichia coli strains isolated from swine feces
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    Foodborne Pathog Dis 5:827-38. 2008
    ..This study demonstrates that swine STEC comprise a heterogeneous group of organisms, and the possession of different combinations of E. coli virulence genes indicate that some swine STEC can potentially cause human illness...
  6. doi request reprint Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
    Foodborne Pathog Dis 8:601-7. 2011
    ..This work provides a method for detection and isolation in ground beef and potentially other foods of non-O157 STEC of major public health concern...
  7. ncbi request reprint Testing of swine feces obtained through the National Animal Health Monitoring System's Swine 2000 study for the presence of Escherichia coli O157:H7
    Ingrid Feder
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 70:1489-92. 2007
    ....
  8. doi request reprint Application of ozonated dry ice (ALIGAL™ Blue Ice) for packaging and transport in the food industry
    Pina M Fratamico
    U S Dept of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Sci 77:M285-91. 2012
    ..Further, ABI can potentially serve as an additional processing hurdle to guard against pathogens during processing, transportation, distribution, and/or storage...
  9. doi request reprint DNA sequence and analysis of a 90.1-kb plasmid in Shiga toxin-producing Escherichia coli (STEC) O145:NM 83-75
    Xianghe Yan
    Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E Mermaid Lane, Wyndmoor, PA 19038, USA
    Plasmid 68:25-32. 2012
    ..coli serotypes O145:NM, O26:H11/H-, O111:NM/H-, and O157:H7 potentially placing these STEC into a related group...
  10. doi request reprint Latex agglutination assays for detection of non-O157 Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145
    Marjorie B Medina
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Prot 75:819-26. 2012
    ..The techniques used to prepare the latex reagents also can be utilized for testing other STEC serogroups, other E. coli serotypes, or other pathogens to ensure safe foods to consumers...
  11. doi request reprint PCR detection of enterohemorrhagic Escherichia coli O145 in food by targeting genes in the E. coli O145 O-antigen gene cluster and the shiga toxin 1 and shiga toxin 2 genes
    Pina M Fratamico
    Microbial Food Safety Research Unit, Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    Foodborne Pathog Dis 6:605-11. 2009
    ..9 x 10(4) CFU/mL, which corresponded to <or=400 CFU/PCR reaction. The PCR assays can be employed to identify enterohemorrhagic E. coli serogroup O145 and to detect low levels of the pathogen in food...
  12. ncbi request reprint Comparison of methods for detection and isolation of cold- and freeze-stressed Escherichia coli O157:H7 in raw ground beef
    Pina M Fratamico
    US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 70:1663-9. 2007
    ..5 times more likely by plating than by the immunoassay or the PCR assay. REB had better overall performance for enrichment of cold- and freeze-stressed E. coli O157:H7 present in ground beef than did the other media examined...
  13. doi request reprint Escherichia coli serogroup O2 and O28ac O-antigen gene cluster sequences and detection of pathogenic E. coli O2 and O28ac by PCR
    Pina M Fratamico
    Eastern Regional Research Center, Agriculture Research Service, US Department of Agriculture, Wyndmoor, PA 19038, USA
    Can J Microbiol 56:308-16. 2010
    ....
  14. doi request reprint Eavesdropping by bacteria: the role of SdiA in Escherichia coli and Salmonella enterica serovar Typhimurium quorum sensing
    James L Smith
    Microbial Food Safety Research Unit, Agricultural Research Service, United States Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    Foodborne Pathog Dis 8:169-78. 2011
    ..However, there is a lack of information concerning the role of SdiA in regulating growth and survival of E. coli and Salmonella in food environments, and therefore studies in this area are needed...
  15. ncbi request reprint Fluoroquinolone resistance in campylobacter
    James L Smith
    Microbial Food Safety Unit, Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038 8598, USA
    J Food Prot 73:1141-52. 2010
    ..Human exposure to Campylobacter infection could be reduced by using strategies that decrease colonization of chickens by the pathogen...
  16. ncbi request reprint Extraintestinal pathogenic Escherichia coli
    James L Smith
    United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA
    Foodborne Pathog Dis 4:134-63. 2007
    ..This review discusses various aspects of ExPEC, including its presence in food products, in animals used for food or as companion pets; the diseases ExPEC can cause; and the virulence factors and virulence mechanisms that cause disease...
  17. ncbi request reprint Effect of temperature on viability of Campylobacter jejuni and Campylobacter coli on raw chicken or pork skin
    Barbara Thurston Solow
    U S Department of Agriculture Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 66:2023-31. 2003
    ..Therefore, a lower incidence of Campylobacter spp. in pork than in poultry postslaughter, despite a similar prevalence in live animals, is not due to differences in viability of C. coli versus C. jejuni on raw chicken or pork skin...
  18. doi request reprint Shiga toxin-producing Escherichia coli
    James L Smith
    USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania, USA
    Adv Appl Microbiol 86:145-97. 2014
    ..Since the non-O157 STEC are responsible for a large portion of STEC-related illnesses, more extensive studies on their physiology, genetics, pathogenicity, and evolution are needed in order to develop more effective control strategies. ..
  19. doi request reprint Effect of stress on non-O157 Shiga toxin-producing Escherichia coli
    James L Smith
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Prot 75:2241-50. 2012
    ....
  20. pmc Characterization of Shiga Toxin Subtypes and Virulence Genes in Porcine Shiga Toxin-Producing Escherichia coli
    Gian Marco Baranzoni
    Eastern Regional Research Center, United States Department of Agriculture Agricultural Research Service Wyndmoor, PA, USA
    Front Microbiol 7:574. 2016
    ..coli with virulence gene profiles associated with human infections. ..
  21. pmc Detection of Shiga toxin-producing Escherichia coli (STEC) O157:H7, O26, O45, O103, O111, O121, and O145, and Salmonella in retail raw ground beef using the DuPont™ BAX® system
    Jamie L Wasilenko
    United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center Wyndmoor, PA, USA United States Department of Agriculture, Agricultural Research Service, Richard Russell Research Center Athens, GA, USA
    Front Cell Infect Microbiol 4:81. 2014
    ..Results demonstrate that the BAX® System assays are effective for detecting STEC and Salmonella in beef. ..
  22. pmc Detection of Shiga toxin-producing Escherichia coli in ground beef using the GeneDisc real-time PCR system
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture Wyndmoor, PA, USA
    Front Cell Infect Microbiol 2:152. 2012
    ..Thus, this work demonstrated that GeneDisc assays are rapid, sensitive, and reliable and can be used for screening ground beef and potentially other foods for STEC serogroups that are important food-borne pathogens worldwide...
  23. doi request reprint Differential gene expression of E. coli O157:H7 in ground beef extract compared to tryptic soy broth
    Pina M Fratamico
    U S Dept of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Food Sci 76:M79-87. 2011
    ..coli O157:H7 differs in TSB compared to GBE. The information will be useful for identification of genes that can be employed as potential targets for interventions to control E. coli O157:H7...
  24. pmc Quorum sensing and production of autoinducer-2 in Campylobacter spp., Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium in foods
    Orla M Cloak
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    Appl Environ Microbiol 68:4666-71. 2002
    ..coli, C. jejuni, Salmonella enterica serovar Typhimurium, and Escherichia coli O157:H7 under different conditions...
  25. ncbi request reprint Performance comparison of a fliC(h7) real-time PCR assay with an H7 latex agglutination test for confirmation of the H type of Escherichia coli O157:H7
    Neelam Narang
    U S Department of Agriculture, Food Safety and Inspection Service, Outbreak Section of the Eastern Laboratory, 950 College Station Road, Athens, Georgia 30605, USA
    J Food Prot 72:2195-7. 2009
    ..coli O157:H7 meat isolates tested negative for the H7 antigen by latex agglutination. The real-time fliC(h7) PCR test can be used to confirm E. coli O157:H7 strains that are not expressing the immunoreactive H7 antigen...
  26. pmc Advances in Molecular Serotyping and Subtyping of Escherichia coli
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor PA, USA
    Front Microbiol 7:644. 2016
    ....
  27. pmc Escherichia coli O-Antigen Gene Clusters of Serogroups O62, O68, O131, O140, O142, and O163: DNA Sequences and Similarity between O62 and O68, and PCR-Based Serogrouping
    Yanhong Liu
    Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, PA 19038, USA
    Biosensors (Basel) 5:51-68. 2015
    ..The PCR assays developed in this study can be used for the detection and identification of E. coli O62/O68, O131, O140, O142, and O163 strains isolated from different sources. ..
  28. ncbi request reprint Comparison of culture, polymerase chain reaction (PCR), TaqMan Salmonella, and Transia Card Salmonella assays for detection of Salmonella spp. in naturally-contaminated ground chicken, ground turkey, and ground beef
    Pina M Fratamico
    Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
    Mol Cell Probes 17:215-21. 2003
    ....
  29. doi request reprint Evaluation of the Performance of the IQ-Check Kits and the USDA Microbiology Laboratory Guidebook Methods for Detection of Shiga Toxin-Producing E. coli (STEC) and STEC and Salmonella Simultaneously in Ground Beef
    Gian Marco Baranzoni
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania, USA
    J Appl Microbiol . 2016
    ..coli (STEC) (O157:H7, O26, O45, O103, O111, O121, and O145) in ground beef and both STEC and Salmonella in co-inoculated samples...
  30. ncbi request reprint Quorum sensing: a primer for food microbiologists
    James L Smith
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 67:1053-70. 2004
    ....
  31. pmc Complete Genome Sequences of Escherichia coli O157:H7 Strains SRCC 1675 and 28RC, Which Vary in Acid Resistance
    Gian Marco Baranzoni
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania, USA
    Genome Announc 4:. 2016
    ..The complete genome sequences belonging to two strains of Escherichia coli O157:H7 with different levels of acid resistance are presented here. ..
  32. pmc Genome sequencing and comparative genomics provides insights on the evolutionary dynamics and pathogenic potential of different H-serotypes of Shiga toxin-producing Escherichia coli O104
    Xianghe Yan
    USDA, Agricultural Research Service, Eastern Regional Research Center, 600 E Mermaid Lane, 19038, Wyndmoor, PA, USA
    BMC Microbiol 15:83. 2015
    ....
  33. doi request reprint From ontology selection and semantic web to an integrated information system for food-borne diseases and food safety
    Xianghe Yan
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    Adv Exp Med Biol 696:741-50. 2011
    ....
  34. doi request reprint The complete DNA sequence and analysis of the virulence plasmid and of five additional plasmids carried by Shiga toxin-producing Escherichia coli O26:H11 strain H30
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 E Mermaid Lane, Wyndmoor, PA 19038, USA
    Int J Med Microbiol 301:192-203. 2011
    ..The data presented underscore the diversity among the STEC virulence plasmids and provide insights into the evolution of these plasmids in STEC strains that cause serious human illness...
  35. doi request reprint Disinfectant and Antimicrobial Susceptibility Profiles of the Big Six Non-O157 Shiga Toxin-Producing Escherichia coli Strains from Food Animals and Humans
    Ross C Beier
    U S Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, Texas 77845, USA
    J Food Prot 79:1355-70. 2016
    ..Bacterial inhibition was not dependent solely on pH but was correlated with the presence of dissociated organic acid species and some undissociated acids. ..
  36. doi request reprint Evaluation of a multiplex real-time PCR method for detecting shiga toxin-producing Escherichia coli in beef and comparison to the U.S. Department of Agriculture Food Safety and Inspection Service Microbiology laboratory guidebook method
    Pina M Fratamico
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 77:180-8. 2014
    ..coli O157:H7 strains in beef. The approach could easily be adapted for additional multiplex assays should regulations expand to include other O serogroups or virulence genes. ..
  37. pmc Phylogenetic identification of bacterial MazF toxin protein motifs among probiotic strains and foodborne pathogens and potential implications of engineered probiotic intervention in food
    Xianghe Yan
    Eastern Regional Research Center, Agricultural Research Service, U, S, Department of Agriculture, 600 E, Mermaid Lane, Wyndmoor, PA, 19038, USA
    Cell Biosci 2:39. 2012
    ..Recently, MazF, the toxin component of the toxin-antitoxin module, has been categorized as an endoribonuclease, or it may have a function similar to that of a RNA interference enzyme...
  38. ncbi request reprint Tolerance to stress and ability of acid-adapted and non-acid-adapted Salmonella enterica serovar Typhimurium DT104 to invade and survive in mammalian cells in vitro
    Pina M Fratamico
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 66:1115-25. 2003
    ....
  39. doi request reprint Scientific status summary
    Bassam A Annous
    U S Dept of Agriculture, Eastern Regional Research Center, Wyndmoor, PA, USA
    J Food Sci 74:R24-37. 2009
    ..The Institute of Food Technologists has issued this Scientific Status Summary to provide readers with a tutorial on biofilms, their purposeful mechanism of interaction (quorum sensing), and recent findings on how to inhibit their formation...
  40. ncbi request reprint A multiplex polymerase chain reaction for the differentiation of Campylobacter jejuni and Campylobacter coli from a swine processing facility and characterization of isolates by pulsed-field gel electrophoresis and antibiotic resistance profiles
    Orla M Cloak
    Microbial Food Safety Research Unit, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA
    J Food Prot 65:266-73. 2002
    ..jejuni and C. coli strains originating from diverse sources were present in porcine samples and in the pork processing plant...
  41. ncbi request reprint Animal and environmental impact on the presence and distribution of Salmonella and Escherichia coli in hydroponic tomato greenhouses
    Leopoldo Orozco R
    Laboratorio de Inocuidad Microbiana de Agua y Alimentos, Departamento de Investigación y Posgrado en Alimentos, Facultad de Quimica, Universidad Autonoma de Queretaro, Queretaro, Qro C P 76000, Mexico
    J Food Prot 71:676-83. 2008
    ..The level of protection provided by hydroponic greenhouses does not exclude the eventuality that enteric pathogenic bacteria can gain access through various avenues...
  42. pmc Species-specific identification of Campylobacters by PCR-restriction fragment length polymorphism and PCR targeting of the gyrase B gene
    Susumu Kawasaki
    National Food Research Institute, Food Hygiene Team, 2 1 12 Kannondai, Tsukuba 305 8642, Japan
    Appl Environ Microbiol 74:2529-33. 2008
    ..Therefore, PCR-RFLP analysis and species-specific PCR assays based on the gyrB gene provide valuable tools for rapid and unambiguous identification of the majority of Campylobacter species...
  43. pmc Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups
    Chitrita Debroy
    Gastroenteric Disease Center, The Pennsylvania State University, Wiley Lab, Wiley Lane, University Park, PA 16802, USA
    Appl Environ Microbiol 71:4919-24. 2005
    ..coli O55 wzx and wzy genes were used to detect the organisms in fecal samples spiked at levels of 10(6) and 10(8) CFU/0.2 g feces. Thus, the PCR assays can be used to detect and identify E. coli serogroups O45 and O55...
  44. pmc Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes
    Chitrita Debroy
    Gastroenteric Disease Center, Department of Veterinary Science, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Appl Environ Microbiol 70:1830-2. 2004
    ..coli O26 and O113 serogroups; these assays will offer an advantage to food and environmental microbiology laboratories in terms of identifying these non-O157 serogroups by replacing antigen-based serotyping...
  45. doi request reprint The application of "omics" technologies for food safety research
    Pina M Fratamico
    Foodborne Pathog Dis 5:369-70. 2008