Daren W Brown
Affiliation: Agricultural Research Service
- A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and Fusarium graminearumD W Brown
Mycotoxin Research Unit, USDA ARS, Peoria, Illinois 61604, USA
Fungal Genet Biol 32:121-33. 2001..Sequence analysis indicated that F. graminearum TRI7 (FgTRI7) is nonfunctional. This is consistent with the fact that the FgTRI7 product is not required for DON synthesis in F. graminearum because C-4 is not oxygenated...
- Inactivation of a cytochrome P-450 is a determinant of trichothecene diversity in Fusarium speciesDaren W Brown
Mycotoxin Research Unit, USDA ARS, National Center for Agricultural Utilization Research, 1815 N University St, Peoria, IL 61604, USA
Fungal Genet Biol 36:224-33. 2002..These genetic observations are consistent with chemical observations that biosynthesis of T-2 toxin and NIV requires a C-4 hydroxylase while biosynthesis of DON does not...
- Genomic analysis of Fusarium verticillioidesD W Brown
Mycotoxin Research Unit, US Department of Agriculture ARS, Peoria, IL, USA
Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:1158-65. 2008..Taken together, these approaches demonstrate the power of microarray technology to provide information on different biological processes...
- Characterization of a fusarium 2-gene cluster involved in trichothecene C-8 modificationDaren W Brown
USDA ARS, National Center for Agricultural Utilization Research, Peoria, Illinois 61604, USA
J Agric Food Chem 51:7936-44. 2003..The presence of the Tri16 pseudogene is consistent with the chemical structure of DON, which has a C-8 keto group rather than the C-8 ester of T-2 toxin...
- Fusarium genomic resources: tools to limit crop diseases and mycotoxin contaminationDaren W Brown
Mycotoxin Research Group, USDA ARS, 1815 N University St, Peoria, IL 61604, USA
Mycopathologia 162:191-9. 2006..Preliminary examination of microarray data collected from F. verticillioides liquid cultures provides evidence of widespread differential gene expression over time...
- Comparative analysis of 87,000 expressed sequence tags from the fumonisin-producing fungus Fusarium verticillioidesDaren W Brown
Mycotoxin Research Unit, U S Department of Agriculture ARS, Peoria, IL 61604, USA
Fungal Genet Biol 42:848-61. 2005..Finally, analysis of an EST that aligns to genomic sequence between FUM12 and FUM13 provided evidence for a previously unidentified gene (FUM20) in the FUM gene cluster...
- The Fusarium verticillioides FUM gene cluster encodes a Zn(II)2Cys6 protein that affects FUM gene expression and fumonisin productionDaren W Brown
Mycotoxin Research Group, USDA ARS, 1815 N University St, Peoria, IL 61604, USA
Eukaryot Cell 6:1210-8. 2007..Based on these data, we present a model for how FUM21 ASFs may regulate fumonisin biosynthesis...
- Functional demarcation of the Fusarium core trichothecene gene clusterDaren W Brown
Mycotoxin Research Unit, USDA ARS, National Center for Agricultural Utilization Research, Peoria, IL 61604, USA
Fungal Genet Biol 41:454-62. 2004..These results provide evidence to demarcate both ends of the core trichothecene gene cluster. Index descriptors: Fungal secondary metabolite, Pathogenic fungi, Gene cluster, Fusarium, Trichothecene, DON..
- Lae1 regulates expression of multiple secondary metabolite gene clusters in Fusarium verticillioidesRobert A E Butchko
National Center for Agricultural Utilization Research, Peoria, IL, United States
Fungal Genet Biol 49:602-12. 2012..Fumonisin production is unchanged in the F. verticillioides lae1 mutant. Complementation of the F. verticillioides mutant resulted in increased fumonisin production...
- Fusarium graminearum TRI14 is required for high virulence and DON production on wheat but not for DON synthesis in vitroRex B Dyer
Mycotoxin Research Unit, USDA ARS, National Center for Agricultural Utilization Research, 1815 N University Street, Peoria, Illinois 61604, USA
J Agric Food Chem 53:9281-7. 2005..We discuss a number of possible roles that TRI14 may play in the disease process...
- Identification of gene clusters associated with fusaric acid, fusarin, and perithecial pigment production in Fusarium verticillioidesDaren W Brown
Bacterial Foodborne Pathogens and Mycology Research, USDA ARS NCAUR, 1815 N University St, Peoria, IL 61604, USA
Fungal Genet Biol 49:521-32. 2012..The results indicate that microarray analysis of RNA derived from culture conditions that induce differential gene expression can be an effective tool for identifying SM biosynthetic gene clusters...
- Fusarium verticillioides chitin synthases CHS5 and CHS7 are required for normal growth and pathogenicityTroy M Larson
Bacterial Foodborne Pathogens and Mycology Research Unit, Agricultural Research Service, U S Department of Agriculture, Peoria, IL 61604 3999, USA
Curr Genet 57:177-89. 2011..Our results demonstrate that both CHS5 and CHS7 are necessary for proper hyphal growth and pathogenicity of F. verticillioides on maize...
- Discontinuous distribution of fumonisin biosynthetic genes in the Gibberella fujikuroi species complexRobert H Proctor
USDA Agriculture Research Service, National Center for Agricultural Utilization Research, Peoria, IL 61604 3999, USA
Mycol Res 108:815-22. 2004..The results indicate the FUM genes are discontinuously distributed in the Gf complex and that this distribution gives rise to the differences in the abilities of closely related Fusarium species to produce fumonisins...
- Impact of temperature stress and validamycin A on compatible solutes and fumonisin production in F. verticillioides: role of trehalose-6-phosphate synthaseBeth A Boudreau
Mund Lagowski Department of Chemistry and Biochemistry, Bradley University, 1501 West Bradley Avenue, Peoria, IL 61625, USA
Fungal Genet Biol 57:1-10. 2013..These data link trehalose biosynthesis, secondary metabolism, and disease, and suggest that trehalose metabolic pathways may be a viable target for the control of Fusarium diseases and fumonisin contamination of maize. ..
- Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformisRobert H Proctor
US Department of Agriculture, National Center for Agricultural Utilization Research, Agriculture Research Service, 1815 North University St, Peoria, IL 61604, USA
Fungal Genet Biol 38:237-49. 2003..Disruption of two of the new genes, encoding longevity assurance factors, had no apparent effect on fumonisin production, but disruption of a third, encoding an ABC transporter, had a subtle effect on ratios of fumonisins produced...
- Patterns of trichothecene production, genetic variability, and virulence to wheat of Fusarium graminearum from smallholder farms in NepalAnne E Desjardins
Mycotoxin Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U S Department of Agriculture, Peoria, IL 61604, USA
J Agric Food Chem 52:6341-6. 2004..These data support the hypothesis that production of deoxynivalenol rather than nivalenol confers a selective advantage to this important wheat pathogen...
- Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusariaKerry O'Donnell
Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
Fungal Genet Biol 52:20-31. 2013..Our results support two competing hypotheses for the circumscription of Fusarium and provide a framework for future comparative phylogenetic and genomic analyses of this agronomically and medically important genus...
- Phylogenomic and functional domain analysis of polyketide synthases in FusariumDaren W Brown
Bacterial Foodborne Pathogens and Mycology Research, USDA ARS NCAUR, Peoria, Illinois 61604, USA
Fungal Biol 116:318-31. 2012..Transcriptional analysis indicates that the 16 F. verticillioides PKS genes are expressed under a range of conditions, further evidence that they are functional genes that confer the ability to produce secondary metabolites...
- The KP4 killer protein gene familyDaren W Brown
USDA ARS NCAUR, Peoria, IL 61604, USA
Curr Genet 57:51-62. 2011..Understanding the nature and function of KP4-like proteins in mycotoxin-producing species like Fusarium may help to limit plant diseases and increase food safety and food production...
- Real-time PCR assay to quantify Fusarium graminearum wild-type and recombinant mutant DNA in plant materialRex B Dyer
USDA Agricultural Research Service, National Center for Agricultural Utilization Research, United States
J Microbiol Methods 67:534-42. 2006..This assay will allow investigators to correlate the amount of disease observed in wheat field trials to the F. graminearum mutant strains being examined...
- FfVel1 and FfLae1, components of a velvet-like complex in Fusarium fujikuroi, affect differentiation, secondary metabolism and virulencePhilipp Wiemann
Institut fur Botanik, Westfalische Wilhelms Universitat Munster, Schlossgarten 3, D 48149 Munster, GermanyInstitut für Lebensmittelchemie, Westfalische Wilhelms Universitat Munster, Corrensstraße 45, D 48149 Munster, GermanyBacterial Foodborne Pathogens and Mycology Research, USDA ARS, 1815 N University St, Peoria, IL 61604, USADepartment of Medical Microbiology and ImmunologyDepartment of Bacteriology, University of Wisconsin, 1550 Linden Dr, Madison, WI 53706 1521, USA
Mol Microbiol 77:972-94. 2010....
- Mycotoxins: A Fungal Genomics PerspectiveDaren W Brown
Mycotoxin Prevention and Applied Microbiology Research, US Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research USDA ARS NCAUR, 1815 North University St, Peoria, IL, 61604, USA
Methods Mol Biol 1542:367-379. 2017..The application of new technologies (e.g., CRISPR) could substantially impact future efforts to harness fungal resources...
- Insights into natural products biosynthesis from analysis of 490 polyketide synthases from FusariumDaren W Brown
Mycotoxin Prevention and Applied Microbiology, U S Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N University St, Peoria, IL 61604, USA Electronic address
Fungal Genet Biol 89:37-51. 2016..The results also highlight ways in which such analyses can help guide identification of novel Fusarium NPs and differences in NP biosynthetic capabilities that exist among fungi. ..
- Cross-species hybridization with Fusarium verticillioides microarrays reveals new insights into Fusarium fujikuroi nitrogen regulation and the role of AreA and NMRBirgit Schönig
Institut für Botanik der Westfälische Wilhelms Universität Münster, Munster, Germany
Eukaryot Cell 7:1831-46. 2008..This is the first report on genome-wide expression studies examining the influence of AreA on nitrogen-responsive gene expression in a genome-wide manner in filamentous fungi...
- Functional analysis of the polyketide synthase genes in the filamentous fungus Gibberella zeae (anamorph Fusarium graminearum)Iffa Gaffoor
Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
Eukaryot Cell 4:1926-33. 2005..Expression of one of the PKS genes was not detected under any of 18 conditions tested. This is the first study to genetically characterize a complete set of PKS genes from a single organism...