Heike Fiegler

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. ncbi Array comparative genomic hybridization analysis of colorectal cancer cell lines and primary carcinomas
    Eleanor J Douglas
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Cancer Res 64:4817-25. 2004
  2. pmc Breaking the waves: improved detection of copy number variation from microarray-based comparative genomic hybridization
    John C Marioni
    Computational Biology Group, Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Centre for Mathematical Sciences, Wilberforce Road, Cambridge CB3 0WA, UK
    Genome Biol 8:R228. 2007
  3. pmc Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11
    Katleen De Preter
    Center for Medical Genetics, Ghent University Hospital, MRB 2nd floor, De Pintelaan 185, B 9000 Ghent, Belgium
    BMC Genomics 6:97. 2005
  4. pmc High resolution array-CGH analysis of single cells
    Heike Fiegler
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nucleic Acids Res 35:e15. 2007
  5. pmc Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes
    Heike Fiegler
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Protoc 2:577-87. 2007
  6. ncbi Deletion at chromosome band 20p12.1 in colorectal cancer revealed by high resolution array comparative genomic hybridization
    Eleanor J Davison
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genes Chromosomes Cancer 44:384-91. 2005
  7. ncbi Genomic array technology
    Heike Fiegler
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Methods Cell Biol 75:769-85. 2004
  8. pmc Accurate and reliable high-throughput detection of copy number variation in the human genome
    Heike Fiegler
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 16:1566-74. 2006
  9. pmc Global variation in copy number in the human genome
    Richard Redon
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 444:444-54. 2006
  10. pmc A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization
    Yeun Jun Chung
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genome Res 14:188-96. 2004

Detail Information

Publications30

  1. ncbi Array comparative genomic hybridization analysis of colorectal cancer cell lines and primary carcinomas
    Eleanor J Douglas
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Cancer Res 64:4817-25. 2004
    ..3 amplification a common finding in MSI+ samples. A number of genes of interest are located within the frequently aberrated regions, which are likely to be of importance in the development and progression of CRC...
  2. pmc Breaking the waves: improved detection of copy number variation from microarray-based comparative genomic hybridization
    John C Marioni
    Computational Biology Group, Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Centre for Mathematical Sciences, Wilberforce Road, Cambridge CB3 0WA, UK
    Genome Biol 8:R228. 2007
    ..However, methods for analyzing the complex data produced and identifying regions of CNV are still being refined...
  3. pmc Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11
    Katleen De Preter
    Center for Medical Genetics, Ghent University Hospital, MRB 2nd floor, De Pintelaan 185, B 9000 Ghent, Belgium
    BMC Genomics 6:97. 2005
    ..We decided to further exploit this model system as a means to identify candidate tumour suppressor or differentiation genes located on chromosome 11...
  4. pmc High resolution array-CGH analysis of single cells
    Heike Fiegler
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nucleic Acids Res 35:e15. 2007
    ..Our results demonstrate the potential of this technology for studies of tumor biology and for clinical diagnostics...
  5. pmc Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changes
    Heike Fiegler
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Protoc 2:577-87. 2007
    ..According to our protocols, the procedure will take approximately 3 days from labeling the DNA to scanning the hybridized slides...
  6. ncbi Deletion at chromosome band 20p12.1 in colorectal cancer revealed by high resolution array comparative genomic hybridization
    Eleanor J Davison
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genes Chromosomes Cancer 44:384-91. 2005
    ..Sequence changes in BA318C17.1 and reduced expression of both genes was detected, suggesting that the abrogation of these genes may play a role in colorectal tumorigenesis...
  7. ncbi Genomic array technology
    Heike Fiegler
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Methods Cell Biol 75:769-85. 2004
  8. pmc Accurate and reliable high-throughput detection of copy number variation in the human genome
    Heike Fiegler
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 16:1566-74. 2006
    ..Based on these studies, we developed a variance-based automatic copy number detection analysis process (CNVfinder) and have demonstrated its robustness by comparison with the SW-ARRAY method...
  9. pmc Global variation in copy number in the human genome
    Richard Redon
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 444:444-54. 2006
    ..The data obtained delineate linkage disequilibrium patterns for many CNVs, and reveal marked variation in copy number among populations. We also demonstrate the utility of this resource for genetic disease studies...
  10. pmc A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization
    Yeun Jun Chung
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genome Res 14:188-96. 2004
    ..The resulting 3K mouse BAC microarrays reproducibly identified DNA copy number alterations in cell lines and primary tumors, such as single-copy deletions, regional amplifications, and aneuploidy...
  11. ncbi Replication timing of human chromosome 6
    Kathryn Woodfine
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Cell Cycle 4:172-6. 2005
    ..Positive correlations are observed between replication timing and a number of genomic features including GC content, repeat content and transcriptional activity...
  12. pmc Germline rates of de novo meiotic deletions and duplications causing several genomic disorders
    Daniel J Turner
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, CB10 1SA, UK
    Nat Genet 40:90-5. 2008
    ....
  13. ncbi Genomic profiling identifies discrete deletions associated with translocations in glioblastoma multiforme
    Paul J Mulholland
    Human Cytogenetics Laboratory, Cancer Research, UK
    Cell Cycle 5:783-91. 2006
    ....
  14. ncbi A complex rearrangement on chromosome 22 affecting both homologues; haplo-insufficiency of the Cat eye syndrome region may have no clinical relevance
    Marjolein Kriek
    Center for Human and Clinical Genetics, Leiden University Medical Center, Einthovenweg, 2300 RC, Leiden, The Netherlands
    Hum Genet 120:77-84. 2006
    ..This study highlights the value of using different genomic approaches to unravel chromosomal alterations in order to study their phenotypic impact...
  15. ncbi Combined array-comparative genomic hybridization and single-nucleotide polymorphism-loss of heterozygosity analysis reveals complex changes and multiple forms of chromosomal instability in colorectal cancers
    Michelle Gaasenbeek
    Molecular and Population Genetics Laboratory, London Research Institute, Cancer Research UK, London WC2A 3PX, UK
    Cancer Res 66:3471-9. 2006
    ..These data suggest that CIN is not synonymous with copy number change and some cancers have a specific tendency to whole-chromosome deletion and regain or to mitotic recombination...
  16. ncbi Guidelines for molecular karyotyping in constitutional genetic diagnosis
    Joris Robert Vermeesch
    Center for Human Genetics, University Hospital Gasthuisberg, Leuven, Belgium
    Eur J Hum Genet 15:1105-14. 2007
    ..Internal and external quality control programmes are urgently needed to evaluate and standardise the test results between laboratories...
  17. ncbi Analysis of ovarian cancer cell lines using array-based comparative genomic hybridization
    Maryou B K Lambros
    Molecular and Population Genetics Laboratory, Cancer Research UK, London, UK
    J Pathol 205:29-40. 2005
    ..Other potential oncogenes, which mapped to regions found by this study, included cyclin E and PIK3C2G. Candidate tumour suppressor genes in regions of loss included CDKN2C, SMAD4-interacting protein and RASSF2...
  18. ncbi High-resolution analysis of genomic copy number alterations in bladder cancer by microarray-based comparative genomic hybridization
    Carolyn D Hurst
    Cancer Research UK Clinical Centre, St James s University Hospital, Beckett St, Leeds LS9 7TF, UK
    Oncogene 23:2250-63. 2004
    ..3 being the most frequent. Real-time PCR analysis revealed a novel candidate gene with consistent overexpression in all cell lines with the 6p22.3 amplicon...
  19. ncbi DNA microarrays for comparative genomic hybridization based on DOP-PCR amplification of BAC and PAC clones
    Heike Fiegler
    Wellcome Trust Sanger Institute Cancer Research UK Genomic Microarray Group, Hinxton, Cambridge, CB10 1SA, United Kingdom
    Genes Chromosomes Cancer 36:361-74. 2003
    ....
  20. pmc Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
    Ewan Birney
    Nature 447:799-816. 2007
    ..Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function...
  21. pmc Replication-timing-correlated spatial chromatin arrangements in cancer and in primate interphase nuclei
    Florian Grasser
    Department of Biology II, Human Genetics, Ludwig Maximilians University Munich, Planegg Martinsreid, Germany
    J Cell Sci 121:1876-86. 2008
    ....
  22. ncbi Determination of donor-type chimerism using a semi-quantitative PCR-based method in a canine model for bone marrow transplantation
    Heike Fiegler
    GSF Forschungszentrum für Umwelt und Gesundheit, Institut für Klinische Molekularbiologie und Tumorgenetik, 81377, Munchen, Germany
    Vet Immunol Immunopathol 84:61-70. 2002
    ..The established semi-quantitative PCR assay offers a quick, simple, accurate and sensitive way of evaluating and quantitating the percentage of chimerism in a sex-mismatched canine BMT model...
  23. ncbi Micro-array analyses decipher exceptional complex familial chromosomal rearrangement
    Christine Fauth
    Institut fur Humangenetik, Technische Universitat Munchen, Trogerstr 32, 81675 Munchen, Germany
    Hum Genet 119:145-53. 2006
    ..We detail the most complicated familial complex chromosomal rearrangement reported to date and thus an extreme example of inheritance of chromosomal rearrangements without error in meiotic segregation...
  24. ncbi Genome-wide screening of genomic alterations and their clinicopathologic implications in non-small cell lung cancers
    Tae Min Kim
    Department of Microbiology, College of Medicine, Catholic University of Korea, Socho gu, Seoul, Korea
    Clin Cancer Res 11:8235-42. 2005
    ..This study screened the genomic aberrations across the whole genome of non-small cell lung cancer cells with high-resolution and investigated their clinicopathologic implications...
  25. ncbi Genomic and protein expression profiling identifies CDK6 as novel independent prognostic marker in medulloblastoma
    Frank Mendrzyk
    Division of Molecular Genetics B060, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    J Clin Oncol 23:8853-62. 2005
    ..Identification of molecular markers for prognosis and development of novel pathogenesis-based therapies depends crucially on a better understanding of medulloblastoma pathomechanisms...
  26. ncbi Investigating chromosome organization with genomic microarrays
    Kathryn Woodfine
    Department of Medical and Molecular Genetics, GKT School of Medicine, King s College London, London, SE1 9RT, UK
    Chromosome Res 13:249-57. 2005
    ..Furthermore, by application of S phase fractions to genomic microarrays, replication timing can be estimated. Thus, microarrays can provide new information about chromosome structure and gene regulation...
  27. ncbi Array-CGH analysis of microsatellite-stable, near-diploid bowel cancers and comparison with other types of colorectal carcinoma
    Angela M Jones
    Molecular and Population Genetics Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Oncogene 24:118-29. 2005
    ..In conclusion, our data support the suggestion that some MSI-CIN- carcinomas form a qualitatively different group from the other cancer types, and also suggest that the MSI-CIN- group is itself heterogeneous...
  28. ncbi Chromatin architecture of the human genome: gene-rich domains are enriched in open chromatin fibers
    Nick Gilbert
    MRC Human Genetics Unit, Edinburgh, EH4 2XU, Scotland
    Cell 118:555-66. 2004
    ..We suggest that domains of open chromatin may create an environment that facilitates transcriptional activation and could provide an evolutionary constraint to maintain clusters of genes together along chromosomes...
  29. ncbi Replication timing of the human genome
    Kathryn Woodfine
    The Welcome Trust Sanger Institute, Welcome Genome Campus, Cambridge, UK
    Hum Mol Genet 13:191-202. 2004
    ..We show a positive correlation, both genome-wide and at a high resolution, between replication timing and a range of genome parameters including GC content, gene density and transcriptional activity...
  30. pmc An integrated resource for genome-wide identification and analysis of human tissue-specific differentially methylated regions (tDMRs)
    Vardhman K Rakyan
    Institute of Cell and Molecular Science, Barts and The London, London E1 2AT, United Kingdom
    Genome Res 18:1518-29. 2008
    ....