Gordon Dougan

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. ncbi request reprint The genome of Salmonella enterica serovar Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    Clin Infect Dis 45:S29-33. 2007
  2. doi request reprint Salmonella enterica serovar Typhi and the pathogenesis of typhoid fever
    Gordon Dougan
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom email
    Annu Rev Microbiol 68:317-36. 2014
  3. pmc RNA-seq reveals the RNA binding proteins, Hfq and RsmA, play various roles in virulence, antibiotic production and genomic flux in Serratia sp. ATCC 39006
    Nabil M Wilf
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK
    BMC Genomics 14:822. 2013
  4. pmc Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism
    Laura J Pettit
    Wellcome Trust Sanger Institute, Hinxton, UK
    BMC Genomics 15:160. 2014
  5. pmc Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium
    Richard A Stabler
    London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Genome Biol 10:R102. 2009
  6. pmc Genomic and phenotypic variation in epidemic-spanning Salmonella enterica serovar Enteritidis isolates
    Laura Betancor
    Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Universidad de la Republica, Montevideo, Uruguay
    BMC Microbiol 9:237. 2009
  7. pmc Searching for the elusive typhoid diagnostic
    Stephen Baker
    Oxford University Clinical Research Unit, The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
    BMC Infect Dis 10:45. 2010
  8. ncbi request reprint How bacteria and their products provide clues to vaccine and adjuvant development
    Gordon Dougan
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Vaccine 24:S2-13-9. 2006
  9. doi request reprint Immunity to salmonellosis
    Gordon Dougan
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK
    Immunol Rev 240:196-210. 2011
  10. pmc Analysis of the hypervariable region of the Salmonella enterica genome associated with tRNA(leuX)
    Anne L Bishop
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Bacteriol 187:2469-82. 2005

Detail Information

Publications131 found, 100 shown here

  1. ncbi request reprint The genome of Salmonella enterica serovar Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    Clin Infect Dis 45:S29-33. 2007
    ..Evidence suggests that, as well as undergoing gene degradation, S. Typhi has also recently acquired genes, such as those encoding the Vi antigen, by horizontal transfer events...
  2. doi request reprint Salmonella enterica serovar Typhi and the pathogenesis of typhoid fever
    Gordon Dougan
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom email
    Annu Rev Microbiol 68:317-36. 2014
    ..Here we review the latest scientific advances in typhoid research and discuss how these novel approaches are changing our understanding of the disease. ..
  3. pmc RNA-seq reveals the RNA binding proteins, Hfq and RsmA, play various roles in virulence, antibiotic production and genomic flux in Serratia sp. ATCC 39006
    Nabil M Wilf
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK
    BMC Genomics 14:822. 2013
    ..Prodigiosin and carbapenem production was abolished, and virulence attenuated, in an S39006 ∆hfq mutant, while the converse was observed in an S39006 rsmA transposon insertion mutant...
  4. pmc Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism
    Laura J Pettit
    Wellcome Trust Sanger Institute, Hinxton, UK
    BMC Genomics 15:160. 2014
    ..The C. difficile spo0A gene encodes a highly conserved transcriptional regulator of sporulation that is required for relapsing disease and transmission in mice...
  5. pmc Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium
    Richard A Stabler
    London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Genome Biol 10:R102. 2009
    ..difficile (CD196), a recent epidemic and hypervirulent 027 (R20291) and a previously sequenced PCR-ribotype 012 strain (630)...
  6. pmc Genomic and phenotypic variation in epidemic-spanning Salmonella enterica serovar Enteritidis isolates
    Laura Betancor
    Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Universidad de la Republica, Montevideo, Uruguay
    BMC Microbiol 9:237. 2009
    ..In this study we investigate genome divergence and pathogenic potential in S. Enteritidis isolated before, during and after an epidemic in Uruguay...
  7. pmc Searching for the elusive typhoid diagnostic
    Stephen Baker
    Oxford University Clinical Research Unit, The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
    BMC Infect Dis 10:45. 2010
    ..Here we discuss the current position of typhoid diagnostics, highlight the need for technological improvements and suggest potential ways of advancing this area...
  8. ncbi request reprint How bacteria and their products provide clues to vaccine and adjuvant development
    Gordon Dougan
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Vaccine 24:S2-13-9. 2006
    ..New knowledge in this field is beginning to explain how vaccine adjuvants work and will facilitate the future development of novel adjuvants and vaccines...
  9. doi request reprint Immunity to salmonellosis
    Gordon Dougan
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK
    Immunol Rev 240:196-210. 2011
    ..Vaccines, both oral and parenteral, are available or under development, and these have been used with some success to explore immunity in both model systems and clinically in humans...
  10. pmc Analysis of the hypervariable region of the Salmonella enterica genome associated with tRNA(leuX)
    Anne L Bishop
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Bacteriol 187:2469-82. 2005
    ..coli, suggesting that these mobile genetic elements have played a major role in driving the variability of this region...
  11. pmc Characterization of the genomes of a diverse collection of Salmonella enterica serovar Typhimurium definitive phage type 104
    Fiona J Cooke
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    J Bacteriol 190:8155-62. 2008
    ..Thus, here the nature of variation in different Salmonella serovar Typhimurium DT104 isolates is further defined at the gene and whole-genome levels, illustrating how this phage type evolves over time...
  12. pmc Evolutionary dynamics of Clostridium difficile over short and long time scales
    Miao He
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 107:7527-32. 2010
    ..1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages...
  13. pmc Pseudogene accumulation in the evolutionary histories of Salmonella enterica serovars Paratyphi A and Typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    BMC Genomics 10:36. 2009
    ..The accumulation of pseudogenes is a key feature of these and other host-adapted pathogens, and overlapping pseudogene complements are evident in Paratyphi A and Typhi...
  14. pmc High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi
    Kathryn E Holt
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Genet 40:987-93. 2008
    ..The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers...
  15. pmc Structure, diversity, and mobility of the Salmonella pathogenicity island 7 family of integrative and conjugative elements within Enterobacteriaceae
    Helena M B Seth-Smith
    Pathogen Genetics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    J Bacteriol 194:1494-504. 2012
    ..We present a growing family of SPI-7-related ICEs whose mobility, abundance, and cargo variability indicate that these elements may have had a large impact on the evolution of the Enterobacteriaceae...
  16. pmc Characterisation of a live Salmonella vaccine stably expressing the Mycobacterium tuberculosis Ag85B-ESAT6 fusion protein
    Lindsay J Hall
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Vaccine 27:6894-904. 2009
    ..Thus, live oral priming with the recombinant Salmonella/Ag85B-ESAT6 and boosting with Ag85B-ESAT6 plus the adjuvant LTK63 represents an effective mucosal vaccination regimen...
  17. pmc Proteomic and genomic characterization of highly infectious Clostridium difficile 630 spores
    Trevor D Lawley
    Microbial Pathogenesis Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    J Bacteriol 191:5377-86. 2009
    ..Thus, our results provide the first molecular definition of C. difficile spores, opening up new opportunities for the development of diagnostic and therapeutic approaches...
  18. pmc High-throughput bacterial SNP typing identifies distinct clusters of Salmonella Typhi causing typhoid in Nepalese children
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    BMC Infect Dis 10:144. 2010
    ..Kathmandu, the capital of Nepal, is an area of high incidence and the pediatric population appears to be at high risk of exposure and infection...
  19. pmc Prophage sequences defining hot spots of genome variation in Salmonella enterica serovar Typhimurium can be used to discriminate between field isolates
    Fiona J Cooke
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Clin Microbiol 45:2590-8. 2007
    ..These multiplex PCR assays, based on prophage-like elements and Salmonella genomic island 1, provide a simple method for identifying new variants of S. enterica serovar Typhimurium in the field...
  20. pmc Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice
    Simon Clare
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Infect Immun 81:723-32. 2013
    ..A similarly susceptible phenotype was observed in μMT mice reconstituted with miR-155-deficient B cells, indicating that miR-155 is required intrinsically for mediating protection against this predominantly luminal bacterial pathogen...
  21. pmc Targeted restoration of the intestinal microbiota with a simple, defined bacteriotherapy resolves relapsing Clostridium difficile disease in mice
    Trevor D Lawley
    Wellcome Trust Sanger Institute, Hinxton, United Kingdom
    PLoS Pathog 8:e1002995. 2012
    ..Further, we demonstrate a rational approach to harness the therapeutic potential of health-associated microbial communities to treat C. difficile disease and potentially other forms of intestinal dysbiosis...
  22. pmc The Clostridium difficile spo0A gene is a persistence and transmission factor
    Laura J Deakin
    Microbial Pathogenesis Laboratory, Wellcome Trust Sanger Institute, Hinxton, United Kingdom
    Infect Immun 80:2704-11. 2012
    ..Thus, the C. difficile Spo0A protein plays a key role in persistent infection, including recurrence and host-to-host transmission in mice...
  23. pmc High-resolution single nucleotide polymorphism analysis distinguishes recrudescence and reinfection in recurrent invasive nontyphoidal Salmonella typhimurium disease
    Chinyere K Okoro
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Clin Infect Dis 54:955-63. 2012
    ..The epidemiology of iNTS is poorly understood. Survivors frequently experience multiply recurrent iNTS disease, despite appropriate antimicrobial therapy, but recrudescence and reinfection have previously been difficult to distinguish...
  24. pmc Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants
    Gemma C Langridge
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2308-16. 2009
    ..Typhi in the gall bladder. This screen validated our hypothesis that we can simultaneously assay every gene in the genome to identify niche-specific essential genes...
  25. pmc A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo
    Angela M Jansen
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002131. 2011
    ..The presence of the Vi capsule also correlated with induction of the anti-inflammatory cytokine IL-10 in vivo, a factor that impacted on chemotaxis and the activation of immune cells in vitro...
  26. pmc A linear plasmid truncation induces unidirectional flagellar phase change in H:z66 positive Salmonella Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Mol Microbiol 66:1207-18. 2007
    ..These data demonstrate that, like some Streptomyces linear elements, at least one of the terminal inverted repeats of pBSSB1 is non-essential, but that a palindromic repeat sequence may be necessary for replication...
  27. pmc Intracontinental spread of human invasive Salmonella Typhimurium pathovariants in sub-Saharan Africa
    Chinyere K Okoro
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Genet 44:1215-21. 2012
    ....
  28. pmc Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux
    Nicola K Petty
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002018. 2011
    ..We draw parallels with the evolution of other bacterial pathogens and conclude that C. rodentium is a recently evolved pathogen that may have emerged alongside the development of inbred mice as a model for human disease...
  29. pmc Emergence of host-adapted Salmonella Enteritidis through rapid evolution in an immunocompromised host
    Elizabeth J Klemm
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Nat Microbiol 1:. 2016
    ..Our analysis identifies common pathways of host adaptation and demonstrates the role that immunocompromised individuals can play in this process...
  30. pmc Phylogeographical analysis of the dominant multidrug-resistant H58 clade of Salmonella Typhi identifies inter- and intracontinental transmission events
    Vanessa K Wong
    1 Wellcome Trust Sanger Institute, Hinxton, UK 2 Department of Microbiology, Addenbrooke s Hospital, Cambridge University Hospitals National Health Service NHS Foundation Trust, Cambridge, UK
    Nat Genet 47:632-9. 2015
    ..This phylogeographical analysis provides a framework to facilitate global management of MDR typhoid and is applicable to similar MDR lineages emerging in other bacterial species. ..
  31. doi request reprint The critical role of histone H2A-deubiquitinase Mysm1 in hematopoiesis and lymphocyte differentiation
    Anastasia Nijnik
    Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Cambridge, United Kingdom
    Blood 119:1370-9. 2012
    ..Overall, these data establish a role for Mysm1 in the maintenance of BM stem cell function, in the control of oxidative stress and genetic stability in hematopoietic progenitors, and in the development of lymphoid and erythroid lineages...
  32. pmc Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1
    Derek Pickard
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Bacteriol 190:2580-7. 2008
    ..The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity...
  33. pmc Genome and transcriptome adaptation accompanying emergence of the definitive type 2 host-restricted Salmonella enterica serovar Typhimurium pathovar
    Robert A Kingsley
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    MBio 4:e00565-13. 2013
    ..Typhimurium isolates alongside phenotypic and transcriptome analyses, to identify genetic signatures linked to host adaptation within the DT2 lineage...
  34. pmc A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar Typhi
    Derek Pickard
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Sulston Building, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Bacteriol 192:5746-54. 2010
    ..Thus, we provide a simple explanation and paradigm of how a diverse group of phages target a single key virulence antigen associated with this important human-restricted pathogen...
  35. pmc A genomewide mutagenesis screen identifies multiple genes contributing to Vi capsular expression in Salmonella enterica serovar Typhi
    Derek Pickard
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    J Bacteriol 195:1320-6. 2013
    ..Transcriptome analysis confirmed a reduction in expression from the viaB locus in these S. Typhi mutant derivatives and defined regulatory networks associated with Vi expression...
  36. pmc The role of sphingosine-1-phosphate transporter Spns2 in immune system function
    Anastasia Nijnik
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Immunol 189:102-11. 2012
    ..Other components of the S1P signaling network are investigated as drug targets for immunosuppressive therapy, but the selective action of Spns2 may present an advantage in this regard...
  37. pmc A novel linear plasmid mediates flagellar variation in Salmonella Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    PLoS Pathog 3:e59. 2007
    ..The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage-related linear plasmid to be described in the Enterobacteriaceae...
  38. pmc Variation in Salmonella enterica serovar typhi IncHI1 plasmids during the global spread of resistant typhoid fever
    Minh Duy Phan
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    Antimicrob Agents Chemother 53:716-27. 2009
    ..We propose that antibiotic selection acts to maintain resistance genes on the plasmid, but there is also competition between plasmids encoding the same resistance phenotype...
  39. pmc A simple method for directional transcriptome sequencing using Illumina technology
    Nicholas J Croucher
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, Cambridgeshire CB10 1SA, UK
    Nucleic Acids Res 37:e148. 2009
    ..Hence, this simple method should prove a useful tool in aiding genome annotation and gene expression studies in both prokaryotes and eukaryotes...
  40. pmc High-throughput genotyping of Salmonella enterica serovar Typhi allowing geographical assignment of haplotypes and pathotypes within an urban District of Jakarta, Indonesia
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    J Clin Microbiol 46:1741-6. 2008
    ..The study also provides insight into the evolution of serovar Typhi and demonstrates the value of a molecular epidemiological technique that is exchangeable, that is internet friendly, and that has global utility...
  41. pmc Emergence of a globally dominant IncHI1 plasmid type associated with multiple drug resistant typhoid
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    PLoS Negl Trop Dis 5:e1245. 2011
    ..Typhi host. The PST6 plasmid conferred the ability to grow in high salt medium (4.7% NaCl), which we demonstrate is due to the presence in PST6 of the Tn6062 transposon encoding BetU...
  42. pmc Signatures of adaptation in human invasive Salmonella Typhimurium ST313 populations from sub-Saharan Africa
    Chinyere K Okoro
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Negl Trop Dis 9:e0003611. 2015
    ..These data define phenotypes that distinguish ST313 isolates from other S. Typhimurium and may represent adaptation to a distinct pathogenesis and lifestyle linked to an-immuno-compromised human population. ..
  43. pmc Mcph1-deficient mice reveal a role for MCPH1 in otitis media
    Jing Chen
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    PLoS ONE 8:e58156. 2013
    ....
  44. pmc Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype
    Robert A Kingsley
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2279-87. 2009
    ..Typhi are either pseudogenes or are absent. Genome analysis of other epidemic ST313 isolates from Malawi and Kenya provided evidence for microevolution and clonal replacement in the field...
  45. pmc Mobilization of the incQ plasmid R300B with a chromosomal conjugation system in Salmonella enterica serovar typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    J Bacteriol 190:4084-7. 2008
    ..Evidence suggests that SPI-7 is susceptible to spontaneous circularization, loss, and transposition. Here, we demonstrate that a region within SPI-7 has the ability to mobilize the small incQ plasmid R300B...
  46. pmc NK cells influence both innate and adaptive immune responses after mucosal immunization with antigen and mucosal adjuvant
    Lindsay J Hall
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom
    J Immunol 184:4327-37. 2010
    ....
  47. pmc A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions
    Carine Makendi
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom
    PLoS Negl Trop Dis 10:e0004446. 2016
    ..Typhimurium reference strain SL1344. Our work outlines novel insights into this important emerging pathogen and provides a baseline understanding for future research studies. ..
  48. pmc Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells
    Raffaella Rossi
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    PLoS ONE 7:e52232. 2012
    ..Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions...
  49. pmc Emergence and global spread of epidemic healthcare-associated Clostridium difficile
    Miao He
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
    Nat Genet 45:109-13. 2013
    ..Our analysis identifies key genetic changes linked to the rapid transcontinental dissemination of epidemic C. difficile 027/BI/NAP1 and highlights the routes by which it spreads through the global healthcare system...
  50. pmc Salmonella bongori provides insights into the evolution of the Salmonellae
    Maria Fookes
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002191. 2011
    ..This work suggests that S. bongori has inherited the ancestral Salmonella virulence gene set, but has adapted by incorporating virulence determinants that resemble those employed by EPEC...
  51. pmc Detecting SNPs and estimating allele frequencies in clonal bacterial populations by sequencing pooled DNA
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Bioinformatics 25:2074-5. 2009
    ....
  52. pmc Reprogramming of T cells to natural killer-like cells upon Bcl11b deletion
    Peng Li
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Science 329:85-9. 2010
    ..Therefore, ITNKs may represent a new cell source for cell-based therapies...
  53. doi request reprint Emergence of host-adapted Salmonella Enteritidis through rapid evolution in an immunocompromised host
    Elizabeth J Klemm
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Microbiol 1:15023. 2016
    ..Our analysis identifies common pathways of host adaptation and demonstrates the role that immunocompromised individuals can play in this process. ..
  54. doi request reprint Single-cell RNA-seq identifies a PD-1hi ILC progenitor and defines its development pathway
    Yong Yu
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nature 539:102-106. 2016
    ..These results provide a perspective for exploring PD-1 and its ligand (PD-L1) in immunotherapy, and allow effective manipulation of the immune system for disease prevention and therapy...
  55. pmc The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development
    Yong Yu
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, England, UK
    J Exp Med 212:865-74. 2015
    ..Inactivation of Bcl11b causes a complete loss of ILC2 development from hematopoietic progenitors, which is confirmed upon immune challenge with either papain administration or influenza virus infection...
  56. pmc A strand-specific RNA-Seq analysis of the transcriptome of the typhoid bacillus Salmonella typhi
    Timothy T Perkins
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    PLoS Genet 5:e1000569. 2009
    ..Typhi OmpR regulon and identify novel OmpR regulated transcripts. Thus, ssRNA-seq provides a novel and powerful approach to the characterization of the bacterial transcriptome...
  57. pmc Candidate live, attenuated Salmonella enterica serotype Typhimurium vaccines with reduced fecal shedding are immunogenic and effective oral vaccines
    Moataz Abd El Ghany
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Infect Immun 75:1835-42. 2007
    ..Further, immunization with serotype Typhimurium aroA candidate vaccines harboring shdA and misL afforded complete protection against challenge with a virulent strain of serotype Typhimurium...
  58. pmc The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli
    Nicola K Petty
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    J Bacteriol 192:525-38. 2010
    ..rodentium and EPEC/EHEC have converged on a common host infection strategy through access to a common pool of mobile DNA and that C. rodentium has lost gene functions associated with a previous pathogenic niche...
  59. pmc ChIP-seq and transcriptome analysis of the OmpR regulon of Salmonella enterica serovars Typhi and Typhimurium reveals accessory genes implicated in host colonization
    Timothy T Perkins
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Mol Microbiol 87:526-38. 2013
    ..SL1068-71 exhibited sequence similarity to sialic acid uptake systems and contributed to colonization of the ileum and caecum in the streptomycin-pretreated mouse model of colitis...
  60. pmc Rapid whole-genome sequencing for investigation of a neonatal MRSA outbreak
    Claudio U Köser
    University of Cambridge, Cambridge, United Kingdom
    N Engl J Med 366:2267-75. 2012
    ..Whole-genome sequencing may provide improved resolution to define transmission pathways and characterize outbreaks...
  61. pmc Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome
    Clara Amid
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    BMC Genomics 10:606. 2009
    ..Defensins have been studied for over two decades and their naming has become a critical issue due to incorrect identification of defensin genes derived from different mouse strains and the duplicated nature of this region...
  62. pmc Rapid pneumococcal evolution in response to clinical interventions
    Nicholas J Croucher
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 331:430-4. 2011
    ..This study details how genomic plasticity within lineages of recombinogenic bacteria can permit adaptation to clinical interventions over remarkably short time scales...
  63. pmc The human transcriptome during nontyphoid Salmonella and HIV coinfection reveals attenuated NFkappaB-mediated inflammation and persistent cell cycle disruption
    Fernanda Schreiber
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    J Infect Dis 204:1237-45. 2011
    ..We previously observed that ex vivo macrophages from HIV-infected subjects challenged with Salmonella Typhimurium exhibit dysregulated proinflammatory cytokine responses...
  64. pmc Evidence for several waves of global transmission in the seventh cholera pandemic
    Ankur Mutreja
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 477:462-5. 2011
    ..Additionally, we show how the acquisition of the SXT family of antibiotic resistance elements has shaped pandemic spread, and show that this family was first acquired at least ten years before its discovery in V. cholerae...
  65. pmc Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways
    Nicholas R Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 18:1624-37. 2008
    ..Enteritidis-harboring mutations in functional homologs of the pseudogenes present in S. Gallinarum could provide an experimentally tractable route toward unraveling the genetic basis of host adaptation in S. enterica...
  66. pmc Genome-Based Infection Tracking Reveals Dynamics of Clostridium difficile Transmission and Disease Recurrence
    Nitin Kumar
    Wellcome Trust Sanger Institute, Hinxton
    Clin Infect Dis 62:746-52. 2016
    ..difficile 027/ST1 lineage, and to distinguish between the 2 causes of recurrent disease, relapse (same strain), or reinfection (different strain)...
  67. pmc The Hd, Hj, and Hz66 flagella variants of Salmonella enterica serovar Typhi modify host responses and cellular interactions
    Fernanda Schreiber
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    Sci Rep 5:7947. 2015
    ..These data highlight important differences in the phenotypic properties of S. Typhi flagella variation and how they impact on the pathogenesis of S. Typhi. ..
  68. pmc Patterns of genome evolution that have accompanied host adaptation in Salmonella
    Gemma C Langridge
    Pathogen Genomics, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 112:863-8. 2015
    ..Gallinarum. Isolates from this second clade were tested in a chick model of infection and exhibited a reduced colonization phenotype, which we postulate represents an intermediate stage in pathogen-host adaptation...
  69. ncbi request reprint Evaluation of a novel Vi conjugate vaccine in a murine model of salmonellosis
    Christine Hale
    The Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK
    Vaccine 24:4312-20. 2006
    ..typhimurium). In contrast, Vi vaccination did not confer protection against oral challenge with virulent Vi-positive S. typhimurium or S. dublin...
  70. pmc Epithelial IL-22RA1-mediated fucosylation promotes intestinal colonization resistance to an opportunistic pathogen
    Tu Anh N Pham
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK
    Cell Host Microbe 16:504-16. 2014
    ..These results support a model whereby IL-22RA1 enhances host-microbiota mutualism to limit detrimental overcolonization by opportunistic pathogens...
  71. pmc Interaction of enteric bacterial pathogens with murine embryonic stem cells
    Jun Yu
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Infect Immun 77:585-97. 2009
    ..Thus, we highlighted the practical use of genetically modified ES cells for studying microbe-host interactions...
  72. pmc Multidrug-resistant Salmonella enterica serovar paratyphi A harbors IncHI1 plasmids similar to those found in serovar typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    J Bacteriol 189:4257-64. 2007
    ..Our data show that these serovar Typhi and Paratyphi A IncHI1 plasmids share highly conserved core DNA and have acquired similar mobile elements encoding antibiotic resistance genes in past decades...
  73. pmc High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease
    Alan W Walker
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    BMC Microbiol 11:7. 2011
    ..We therefore specifically sought to compare the mucosa-associated microbiota from both inflamed and non-inflamed sites of the colon in CD and UC patients to that from non-IBD controls and to detect disease-specific profiles...
  74. pmc Use of purified Clostridium difficile spores to facilitate evaluation of health care disinfection regimens
    Trevor D Lawley
    Microbial Pathogenesis Laboratory, Wellcome Trust, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Appl Environ Microbiol 76:6895-900. 2010
    ..Thus, pure C. difficile spores facilitate practical methods for evaluating the efficacy of C. difficile spore disinfection regimens and bringing scientific acumen to C. difficile infection control...
  75. pmc Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of Vietnam
    Kathryn E Holt
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Negl Trop Dis 5:e929. 2011
    ..Typhi isolated from 267 typhoid fever patients in the Mekong delta region participating in a randomized trial conducted between 2004 and 2005...
  76. ncbi request reprint The role of prophage-like elements in the diversity of Salmonella enterica serovars
    Nicholas Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Mol Biol 339:279-300. 2004
    ..Lysogenic bacteriophages therefore play a crucial role in the generation of genetic diversity within S.enterica...
  77. pmc Detection of Vi-negative Salmonella enterica serovar typhi in the peripheral blood of patients with typhoid fever in the Faisalabad region of Pakistan
    Stephen Baker
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    J Clin Microbiol 43:4418-25. 2005
    ..The method described here can be used to monitor the incidence of Vi-negative serovar Typhi in regions where the Vi vaccine is used...
  78. pmc Requirement of bic/microRNA-155 for normal immune function
    Antony Rodriguez
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 316:608-11. 2007
    ..Our work suggests that bic/microRNA-155 plays a key role in the homeostasis and function of the immune system...
  79. pmc Characterization of the yehUT two-component regulatory system of Salmonella enterica Serovar Typhi and Typhimurium
    Vanessa K Wong
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom University of Cambridge Department of Pathology, Addenbrooke s Hospital, Cambridge, United Kingdom
    PLoS ONE 8:e84567. 2013
    ..Typhi and S. Typhimurium) or induce colitis in a murine model (S. Typhimurium only). Growth, metabolic and antimicrobial susceptibility tests identified no obvious influences of yehUT on these phenotypes. ..
  80. pmc IFITM3 restricts the morbidity and mortality associated with influenza
    Aaron R Everitt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Nature 484:519-23. 2012
    ..Together these data reveal that the action of a single intrinsic immune effector, IFITM3, profoundly alters the course of influenza virus infection in mouse and humans...
  81. pmc Mannosidase 2, alpha 1 deficiency is associated with ricin resistance in embryonic stem (ES) cells
    Wei Wang
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom
    PLoS ONE 6:e22993. 2011
    ..Therefore, we conclusively identified mannosidase 2, alpha 1 deficiency to be associated with ricin resistance...
  82. pmc Antibiotic treatment of clostridium difficile carrier mice triggers a supershedder state, spore-mediated transmission, and severe disease in immunocompromised hosts
    Trevor D Lawley
    Microbial Pathogenesis Laboratory1 and Pathogen Genomics, Hinxton, United Kingdom
    Infect Immun 77:3661-9. 2009
    ..difficile and the host and its microbiota, and the results obtained should guide infection control measures...
  83. pmc Distinctive profiles of infection and pathology in hamsters infected with Clostridium difficile strains 630 and B1
    David Goulding
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Infect Immun 77:5478-85. 2009
    ..However, pilus-like structures were detected on 630 located in the crypts of the gut tissue. Furthermore, B1 bacteria, but not 630 bacteria, were found within nonphagocytic cells, including enterocytes and muscle cells...
  84. pmc Vi antigen expression in Salmonella enterica serovar Typhi clinical isolates from Pakistan
    John Wain
    Investigative Sciences, Centre for Molecular Microbiology and Infection, Imperial College, London, United Kingdom
    J Clin Microbiol 43:1158-65. 2005
    ....
  85. pmc Probing local innate immune responses after mucosal immunisation
    Lindsay J Hall
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    J Immune Based Ther Vaccines 8:5. 2010
    ..Intranasal immunisation is potentially a very effective route for inducing both mucosal and systemic immunity to an infectious agent...
  86. pmc Increased susceptibility of C1q-deficient mice to Salmonella enterica serovar Typhimurium infection
    Joanna Warren
    Rheumatology Section, Division of Medicine, Department of Biochemistry, Imperial College School of Medicine London, University of Cambridge, Cambridge, United Kingdom
    Infect Immun 70:551-7. 2002
    ..The data presented here suggest the possibility of novel pathways by which C1q may modulate the pathogenesis of infectious diseases caused by intracellular pathogens...
  87. ncbi request reprint The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
    Mohammed Sebaihia
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Genet 38:779-86. 2006
    ..The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism...
  88. pmc An investigation of clinical and immunological events following repeated aerodigestive tract challenge infections with live Mycobacterium bovis Bacille Calmette Guérin
    Fernanda Schreiber
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Vaccine 28:5427-31. 2010
    ..Oral challenge with BCG Moreau Rio de Janeiro vaccine was immunogenic in healthy volunteers, limiting its potential to explore clinical innate immune responses, but with low reactogenicity...
  89. pmc The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081
    Nicholas R Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Genet 2:e206. 2006
    ..Moreover, wider investigations looking at the patterns of gene loss and gain in the Yersinia have highlighted common themes in the genome evolution of other human enteropathogens...
  90. ncbi request reprint Development of attenuated Salmonella strains that express heterologous antigens
    Frances Bowe
    Centre for Molecular Microbiology and Immunology, Department of Biological Sciences, Imperial College, London, United Kingdom
    Methods Mol Med 87:83-100. 2003
  91. pmc Molecular analysis of incHI1 antimicrobial resistance plasmids from Salmonella serovar Typhi strains associated with typhoid fever
    John Wain
    Wellcome Trust Clinical Research Unit, Department of Biological Sciences, Imperial College, London
    Antimicrob Agents Chemother 47:2732-9. 2003
    ..In 1996 a single RFLP type, RFLP7, was found to carry the dfrA7 and sul-1 genes, which were not present on R27 or pHCM1. This plasmid type appears to have a selective advantage over other plasmids with the same resistance phenotype...
  92. pmc Composition, acquisition, and distribution of the Vi exopolysaccharide-encoding Salmonella enterica pathogenicity island SPI-7
    Derek Pickard
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Armstrong Road, London SW7 2AZ, UK
    J Bacteriol 185:5055-65. 2003
    ..citri. This analysis suggested that SPI-7 may be a mobile element, such as a conjugative transposon or an integrated plasmid remnant...
  93. ncbi request reprint A clinical, microbiological, and pathological study of intestinal perforation associated with typhoid fever
    Quoc Chanh Nguyen
    Dong Thap Provincial Hospital, Dong Thap, Ho Chi Minh City, Vietnam
    Clin Infect Dis 39:61-7. 2004
    ..Immunohistochemical results showed that the predominant infiltrating cell types at the site of perforation were CD68+ leukocytes (macrophages) or CD3+ leukocytes (T lymphocytes)...
  94. ncbi request reprint Unlocking the genome of the human typhoid bacillus
    John Wain
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, UK
    Lancet Infect Dis 2:163-70. 2002
    ..We have new insights into the mechanisms underpinning the human host specificity of S typhi, and have exploitable new routes to improved diagnostics and a better understanding of the epidemiology of the disease...
  95. pmc Characterization of Salmonella enterica derivatives harboring defined aroC and Salmonella pathogenicity island 2 type III secretion system (ssaV) mutations by immunization of healthy volunteers
    Zoe Hindle
    Microscience, Wokingham Berkshire RG41 5TU, United Kingdom
    Infect Immun 70:3457-67. 2002
    ..These data indicate that mutations in type III secretion systems may provide a route to the development of live vaccines in humans and highlight significant differences in the potential use of serovars Typhimurium and Typhi...
  96. ncbi request reprint Cytokine release by lipopolysaccharide-stimulated whole blood from patients with typhoid fever
    Deborah House
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology, and Medicine, London, United Kingdom
    J Infect Dis 186:240-5. 2002
    ..In acute typhoid fever, the ability of peripheral blood leukocytes to release proinflammatory cytokines in response to an inflammatory stimulus is depressed, and this may contribute to delayed recovery following antibiotic treatment...
  97. ncbi request reprint Modulation of dendritic cell endocytosis and antigen processing pathways by Escherichia coli heat-labile enterotoxin and mutant derivatives
    Liljana Petrovska
    Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Sciences, 46 Cleveland Street, London W1P 6DB, UK
    Vaccine 21:1445-54. 2003
    ..This response of dendritic cells to toxin is likely to play an important role in determining the adjuvant activity of these molecules...
  98. pmc Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, College Station 77843 1114, USA
    Infect Immun 71:629-40. 2003
    ..These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice...
  99. ncbi request reprint Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2
    Claire Kidgell
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK
    Plasmid 47:159-71. 2002
    ..typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world...
  100. ncbi request reprint Chronic bacterial infections: living with unwanted guests
    Douglas Young
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Nat Immunol 3:1026-32. 2002
    ..Bacteria appear to adapt their immediate environment to favor survival and may hijack essential immunoregulatory mechanisms designed to minimize immune pathology or the inappropriate activation of immune effectors...
  101. ncbi request reprint Typhoid fever
    Christopher M Parry
    Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom
    N Engl J Med 347:1770-82. 2002