Donald Wlodkowic

Summary

Affiliation: University of Glasgow
Country: UK

Publications

  1. pmc Real-time cytotoxicity assays
    Donald Wlodkowic
    The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Methods Mol Biol 731:285-91. 2011
  2. doi request reprint Microfluidic cell arrays in tumor analysis: new prospects for integrated cytomics
    Donald Wlodkowic
    The Bioelectronics Research Centre, University of Glasgow, Rankine Building, Oakfiled Avenue, Glasgow, G12 8LT, UK
    Expert Rev Mol Diagn 10:521-30. 2010
  3. pmc Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry
    Donald Wlodkowic
    Department of Electronics and Electrical Engineering, The Bioelectronics Research Center, University of Glasgow, Glasgow, UK
    Exp Cell Res 315:1706-14. 2009
  4. doi request reprint Microfabricated analytical systems for integrated cancer cytomics
    Donald Wlodkowic
    The Bioelectronics Research Centre, University of Glasgow, Oakfield Avenue, Glasgow G12 8LT, UK
    Anal Bioanal Chem 398:193-209. 2010
  5. pmc Cytometry in cell necrobiology revisited. Recent advances and new vistas
    Donald Wlodkowic
    The Bioelectronics Research Centre, University of Glasgow, Glasgow, United Kingdom
    Cytometry A 77:591-606. 2010
  6. pmc Kinetic viability assays using DRAQ7 probe
    Donald Wlodkowic
    The BioMEMS Research Group, School of Chemical Sciences, University of Auckland, Auckland, New Zealand
    Curr Protoc Cytom . 2013
  7. pmc Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells
    Donald Wlodkowic
    The Bioelectronics Research Center, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT Glasgow, UK
    Anal Chem 81:6952-9. 2009
  8. doi request reprint Biological implications of polymeric microdevices for live cell assays
    Donald Wlodkowic
    Department of Electronics and Electrical Engineering, The Bioelectronics Research Centre, University of Glasgow, Glasgow, UK G12 8LT
    Anal Chem 81:9828-33. 2009
  9. pmc Apoptosis and beyond: cytometry in studies of programmed cell death
    Donald Wlodkowic
    Biomems Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Methods Cell Biol 103:55-98. 2011
  10. pmc Microfluidic single-cell array cytometry for the analysis of tumor apoptosis
    Donald Wlodkowic
    The Bioelectronics Research Centre, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT, United Kingdom
    Anal Chem 81:5517-23. 2009

Collaborators

Detail Information

Publications32

  1. pmc Real-time cytotoxicity assays
    Donald Wlodkowic
    The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Methods Mol Biol 731:285-91. 2011
    ..Considering vast time savings and kinetic data acquisition such assays have the potential to be applied in a number of areas including accelerated anticancer drug discovery and high-throughput screening routines...
  2. doi request reprint Microfluidic cell arrays in tumor analysis: new prospects for integrated cytomics
    Donald Wlodkowic
    The Bioelectronics Research Centre, University of Glasgow, Rankine Building, Oakfiled Avenue, Glasgow, G12 8LT, UK
    Expert Rev Mol Diagn 10:521-30. 2010
    ..Lab-on-a-chip microfluidic cell arrays therefore provide new opportunities for the development of content-rich personalized clinical diagnostics and higher-throughput clinical screening in oncology...
  3. pmc Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry
    Donald Wlodkowic
    Department of Electronics and Electrical Engineering, The Bioelectronics Research Center, University of Glasgow, Glasgow, UK
    Exp Cell Res 315:1706-14. 2009
    ..In particular, it should allow for dynamic analysis of samples where low cell number has so far been an obstacle, e.g. primary cancer stems cells or circulating minimal residual tumors...
  4. doi request reprint Microfabricated analytical systems for integrated cancer cytomics
    Donald Wlodkowic
    The Bioelectronics Research Centre, University of Glasgow, Oakfield Avenue, Glasgow G12 8LT, UK
    Anal Bioanal Chem 398:193-209. 2010
    ..It is largely anticipated that advances in microfluidic technologies should aid in tailoring of investigational therapies and support the current computational efforts in systems biology...
  5. pmc Cytometry in cell necrobiology revisited. Recent advances and new vistas
    Donald Wlodkowic
    The Bioelectronics Research Centre, University of Glasgow, Glasgow, United Kingdom
    Cytometry A 77:591-606. 2010
    ..This review highlights the key developments warranting a renaissance and evolution of cytometric techniques in the field of cell necrobiology...
  6. pmc Kinetic viability assays using DRAQ7 probe
    Donald Wlodkowic
    The BioMEMS Research Group, School of Chemical Sciences, University of Auckland, Auckland, New Zealand
    Curr Protoc Cytom . 2013
    ....
  7. pmc Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells
    Donald Wlodkowic
    The Bioelectronics Research Center, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT Glasgow, UK
    Anal Chem 81:6952-9. 2009
    ....
  8. doi request reprint Biological implications of polymeric microdevices for live cell assays
    Donald Wlodkowic
    Department of Electronics and Electrical Engineering, The Bioelectronics Research Centre, University of Glasgow, Glasgow, UK G12 8LT
    Anal Chem 81:9828-33. 2009
    ..Results show that PDMS is nontoxic to cells, as their viability and growth characteristic in PDMS-based platforms is comparable to that of their polystyrene counterparts...
  9. pmc Apoptosis and beyond: cytometry in studies of programmed cell death
    Donald Wlodkowic
    Biomems Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Methods Cell Biol 103:55-98. 2011
    ..Strategies of selecting the optimal multiparameter approaches, as well as potential difficulties in the experimental procedures, are thoroughly summarized...
  10. pmc Microfluidic single-cell array cytometry for the analysis of tumor apoptosis
    Donald Wlodkowic
    The Bioelectronics Research Centre, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT, United Kingdom
    Anal Chem 81:5517-23. 2009
    ....
  11. doi request reprint Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis
    Khashayar Khoshmanesh
    Department of Chemistry and MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand
    Anal Chem 83:3217-21. 2011
    ..This work provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells...
  12. doi request reprint Toward embedded laboratory automation for smart Lab-on-a-Chip embryo arrays
    Kevin I Kai Wang
    Department of Electrical and Computer Engineering, University of Auckland, Auckland, New Zealand
    Biosens Bioelectron 48:188-96. 2013
    ..This work presents evidence that integration of embedded electronic interfaces with microfluidic chip-based technologies can bring the Lab-on-a-Chip a step closer to fully automated analytical systems...
  13. pmc Real-time cell viability assays using a new anthracycline derivative DRAQ7®
    Jin Akagi
    The BioMEMS Research Group, School of Chemical Sciences, University of Auckland, Auckland, New Zealand
    Cytometry A 83:227-34. 2013
    ..We provide evidence that this low-dosage, real-time labeling procedure provides multiparameter and kinetic fingerprint of anticancer drug action...
  14. pmc Miniaturized embryo array for automated trapping, immobilization and microperfusion of zebrafish embryos
    Jin Akagi
    The BioMEMS Research Group, School of Chemical Sciences, University of Auckland, Auckland, New Zealand
    PLoS ONE 7:e36630. 2012
    ..The work provides a new rationale for rapid and automated manipulation and analysis of developing zebrafish embryos at a large scale...
  15. pmc Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
    Donald Wlodkowic
    The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland 1142, New Zealand
    Methods Mol Biol 740:81-9. 2011
    ....
  16. doi request reprint Wormometry-on-a-chip: Innovative technologies for in situ analysis of small multicellular organisms
    Donald Wlodkowic
    Department of Chemistry and MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, 1142, New Zealand
    Cytometry A 79:799-813. 2011
    ..We also introduce a new term "wormometry" to collectively distinguish these up-and-coming chip-based technologies that go far beyond the conventional meaning of the term "cytometry."..
  17. doi request reprint Multiparameter Lab-on-a-Chip flow cytometry of the cell cycle
    Joanna Skommer
    The BioMEMS Research Group, School of Chemical Sciences, University of Auckland, Auckland, New Zealand
    Biosens Bioelectron 42:586-91. 2013
    ..They can be a viable alternative to conventional FCM for multiparameter detection of programmed cell death...
  18. doi request reprint Trapping and imaging of micron-sized embryos using dielectrophoresis
    Khashayar Khoshmanesh
    The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Electrophoresis 32:3129-32. 2011
    ..This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force...
  19. pmc Flow cytometry-based apoptosis detection
    Donald Wlodkowic
    The Bioelectronics Research Center, University of Glasgow, Rankine Building, Glasgow G12 8LT, UK
    Methods Mol Biol 559:19-32. 2009
    ..In the present chapter, we outline a handful of commonly used methods that are based on the assessment of: mitochondrial transmembrane potential, activation of caspases, plasma membrane alterations and DNA fragmentation...
  20. pmc Cell death goes LIVE: technological advances in real-time tracking of cell death
    Joanna Skommer
    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    Cell Cycle 9:2330-41. 2010
    ..Here, we review current status of the most innovative analytical technologies for dynamic tracking of cell death, and address the interdisciplinary promises and future challenges of these methods...
  21. doi request reprint Multiparameter detection of apoptosis using red-excitable SYTO probes
    Donald Wlodkowic
    Department of Biological Sciences, Glasgow Caledonian University, Glasgow, United Kingdom
    Cytometry A 73:563-9. 2008
    ..Considering simplicity of staining protocols, low cost, and avoidance of spectral compensation problems, we expect that red-excitable SYTO dyes will find a wide range of cytometric applications...
  22. pmc Apoptosis goes on a chip: advances in the microfluidic analysis of programmed cell death
    Donald Wlodkowic
    The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Anal Chem 83:6439-46. 2011
    ....
  23. ncbi request reprint SYTO probes: markers of apoptotic cell demise
    Donald Wlodkowic
    University of Glasgow, UK
    Curr Protoc Cytom . 2007
    ....
  24. doi request reprint Dynamic analysis of drug-induced cytotoxicity using chip-based dielectrophoretic cell immobilization technology
    Khashayar Khoshmanesh
    Department of Chemistry and MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand
    Anal Chem 83:2133-44. 2011
    ..This ability is of particular importance in studying the outcome of patient derived cancer cells, when exposed to therapeutic drugs, as these cells are often rare and difficult to collect, purify and immobilize...
  25. pmc Rise of the micromachines: microfluidics and the future of cytometry
    Donald Wlodkowic
    The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Methods Cell Biol 102:105-25. 2011
    ....
  26. doi request reprint SYTO probes in the cytometry of tumor cell death
    Donald Wlodkowic
    The Bioelectronics Research Center, University of Glasgow, Glasgow, United Kingdom
    Cytometry A 73:496-507. 2008
    ..Finally, we provide a future outlook on SYTO probes in cytometric and microfluidics (Lab-on-a-Chip) high content analysis applications...
  27. doi request reprint Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells
    Shannon L Faley
    Bioelectronics Research Centre, University of Glasgow, Oakfield Avenue, Glasgow, UK G12 8LT
    Lab Chip 9:2659-64. 2009
    ..Further studies show that dasatinib does, however, markedly reduce CML stem/progenitor cell migration in situ...
  28. pmc ER-Golgi network--a future target for anti-cancer therapy
    Donald Wlodkowic
    Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, UK
    Leuk Res 33:1440-7. 2009
    ..As the interest in the role of ER-Golgi network during cancer cell death has been gaining momentum, we attempt here to critically appraise current status of development of investigational anti-cancer agents that target ER and/or Golgi...
  29. doi request reprint Multiparameter analysis of apoptosis using lab-on-a-chip flow cytometry
    Donald Wlodkowic
    The BioMEMS Research Group, School of Chemical Sciences, University of Auckland, Auckland, New Zealand The BioMEMS Research Group, School of Applied Sciences, RMIT University, Melbourne, Australia
    Curr Protoc Cytom 66:Unit 9.42.. 2013
    ..Finally, we describe the combined use of fluorogenic caspases substrate PhiPhiLux with DRAQ5 probe to measure caspase activation in relation to the cell cycle profile in living tumor cells...
  30. ncbi request reprint Timing is everything: stochastic origins of cell-to-cell variability in cancer cell death
    Joanna Skommer
    School of Biological Sciences, University of Auckland, Auckland 1142, New Zealand
    Front Biosci (Landmark Ed) 16:307-14. 2011
    ..Here we summarize our current understanding of the origins of stochastic variability in cells' commitment to apoptosis, and its implications in the field on cancer therapy...
  31. doi request reprint Tumors on chips: oncology meets microfluidics
    Donald Wlodkowic
    Auckland Microfabrication Facility, Department of Chemistry, University of Auckland, Auckland, New Zealand
    Curr Opin Chem Biol 14:556-67. 2010
    ..It is envisaged that microfluidic solutions may well represent the platform of choice for next generation in vitro cancer models...
  32. pmc Microfluidics: Emerging prospects for anti-cancer drug screening
    Donald Wlodkowic
    Donald Wlodkowic, Auckland Microfabrication Facility, Department of Chemistry, University of Auckland, 1142 Auckland, New Zealand
    World J Clin Oncol 1:18-23. 2010
    ..Herein we highlight the selected advancements in this emerging field of bioengineering, and provide a snapshot of developments with relevance to anti-cancer drug screening routines...