P Lewis White

Summary

Affiliation: University Hospital of Wales
Country: UK

Publications

  1. pmc Detection of Candida in concentrated oral rinse cultures by real-time PCR
    P Lewis White
    Department of Medical Microbiology and PHLS, University Hospital of Wales, Cardiff, United Kingdom
    J Clin Microbiol 42:2101-7. 2004
  2. pmc Comparison of non-culture-based methods for detection of systemic fungal infections, with an emphasis on invasive Candida infections
    P Lewis White
    Department of Medical Microbiology and NPHS, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, United Kingdom
    J Clin Microbiol 43:2181-7. 2005
  3. ncbi request reprint The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting
    P Lewis White
    Department of Medical Microbiology, National Public Health Service, Cardiff, United Kingdom
    Clin Infect Dis 42:479-86. 2006
  4. pmc A consensus on fungal polymerase chain reaction diagnosis?: a United Kingdom-Ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections
    P Lewis White
    Department of Medical Microbiology and NPHS Cardiff, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, UK
    J Mol Diagn 8:376-84. 2006
  5. pmc Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens
    P Lewis White
    Public Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom
    J Clin Microbiol 48:3753-5. 2010
  6. pmc Aspergillus PCR: one step closer to standardization
    P Lewis White
    NPHS Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, United Kingdom
    J Clin Microbiol 48:1231-40. 2010
  7. doi request reprint An update on the molecular diagnosis of invasive fungal disease
    P Lewis White
    NPHS Microbiology Cardiff, UHW, Heath Park, Cardiff, UK
    FEMS Microbiol Lett 296:1-10. 2009
  8. pmc Evaluation of Aspergillus PCR protocols for testing serum specimens
    P Lewis White
    PHW Microbiology Cardiff, UK
    J Clin Microbiol 49:3842-8. 2011
  9. pmc Evaluation of analytical and preliminary clinical performance of Myconostica MycAssay Aspergillus when testing serum specimens for diagnosis of invasive Aspergillosis
    P Lewis White
    Public Health Wales, Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, United Kingdom
    J Clin Microbiol 49:2169-74. 2011
  10. pmc Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative
    P Lewis White
    Public Health Wales Microbiology Cardiff, Cardiff, United Kingdom
    J Clin Microbiol 53:2832-7. 2015

Detail Information

Publications21

  1. pmc Detection of Candida in concentrated oral rinse cultures by real-time PCR
    P Lewis White
    Department of Medical Microbiology and PHLS, University Hospital of Wales, Cardiff, United Kingdom
    J Clin Microbiol 42:2101-7. 2004
    ..A total of 32 CRC specimens were Candida free by all methods. In summary, a real-time PCR that provides a sensitive, specific, and rapid alternative technique for detection of Candida in the mouth is described...
  2. pmc Comparison of non-culture-based methods for detection of systemic fungal infections, with an emphasis on invasive Candida infections
    P Lewis White
    Department of Medical Microbiology and NPHS, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, United Kingdom
    J Clin Microbiol 43:2181-7. 2005
    ..In conclusion, both PCR and ELISA techniques should be used in unison to aid the detection of invasive fungal infections...
  3. ncbi request reprint The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting
    P Lewis White
    Department of Medical Microbiology, National Public Health Service, Cardiff, United Kingdom
    Clin Infect Dis 42:479-86. 2006
    ..Invasive aspergillosis (IA) is associated with high mortality. Successful outcome with treatment is linked to early diagnosis. The utility of classic diagnostic methods, however, is limited...
  4. pmc A consensus on fungal polymerase chain reaction diagnosis?: a United Kingdom-Ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections
    P Lewis White
    Department of Medical Microbiology and NPHS Cardiff, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, UK
    J Mol Diagn 8:376-84. 2006
    ..Therefore, the preferred Aspergillus method relied on the amplification platform available to the user. This study represents the initial process to achieve a consensus method for the diagnosis of IFIs...
  5. pmc Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens
    P Lewis White
    Public Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom
    J Clin Microbiol 48:3753-5. 2010
    ..The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance...
  6. pmc Aspergillus PCR: one step closer to standardization
    P Lewis White
    NPHS Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, United Kingdom
    J Clin Microbiol 48:1231-40. 2010
    ..DNA should be eluted in volumes of <100 microl...
  7. doi request reprint An update on the molecular diagnosis of invasive fungal disease
    P Lewis White
    NPHS Microbiology Cardiff, UHW, Heath Park, Cardiff, UK
    FEMS Microbiol Lett 296:1-10. 2009
    ..It is by no means a systematic review of the literature but is intended, where possible, to provide the reader with access to assays with proficient clinical performance...
  8. pmc Evaluation of Aspergillus PCR protocols for testing serum specimens
    P Lewis White
    PHW Microbiology Cardiff, UK
    J Clin Microbiol 49:3842-8. 2011
    ..Most Aspergillus PCR protocols used to test serum generate satisfactory analytical performance. Testing serum requires less standardization, and the specific recommendations shown in this article will only improve performance...
  9. pmc Evaluation of analytical and preliminary clinical performance of Myconostica MycAssay Aspergillus when testing serum specimens for diagnosis of invasive Aspergillosis
    P Lewis White
    Public Health Wales, Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff CF14 4XN, United Kingdom
    J Clin Microbiol 49:2169-74. 2011
    ..The performance of the MAP assay is comparable to that of the IHP assay and to those in previously reported studies evaluating commercial tests (galactomannan enzyme-linked immunosorbent assay)...
  10. pmc Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative
    P Lewis White
    Public Health Wales Microbiology Cardiff, Cardiff, United Kingdom
    J Clin Microbiol 53:2832-7. 2015
    ..PCR positivity occurs earlier when testing plasma and provides sufficient sensitivity for the screening of invasive aspergillosis while maintaining methodological simplicity. ..
  11. doi request reprint Prevention and diagnosis of invasive fungal disease in high-risk patients within an integrative care pathway
    Rosemary A Barnes
    Department of Medical Microbiology and Infectious Diseases, Institute of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    J Infect 67:206-14. 2013
    ....
  12. pmc Comparison of galactomannan enzyme immunoassay performance levels when testing serum and plasma samples
    P Lewis White
    Public Health Wales, Microbiology Cardiff, University Hospital of Wales UHW, Cardiff, United Kingdom
    Clin Vaccine Immunol 20:636-8. 2013
    ..7%) and specificity (85.4%) comparable to those for serum. Plasma index values were higher than those for serum; if plasma GM EIA were used to define probable cases, four additional cases would have been diagnosed...
  13. pmc Comparison of Nonculture Blood-Based Tests for Diagnosing Invasive Aspergillosis in an Animal Model
    P Lewis White
    PHW Microbiology, Cardiff, United Kingdom
    J Clin Microbiol 54:960-6. 2016
    ..By combining multiple tests, IA can be excluded or confirmed, highlighting the need for a combined diagnostic strategy. However, this approach must be balanced against the practicality and cost of using multiple tests. ..
  14. ncbi request reprint Detection of seven Candida species using the Light-Cycler system
    P Lewis White
    Department of Medical Microbiology and PHLS, University Hospital Wales, Heath Park, Cardiff CF14 4XN, UK
    J Med Microbiol 52:229-38. 2003
    ....
  15. pmc Evaluation of real-time PCR, galactomannan enzyme-linked immunosorbent assay (ELISA), and a novel lateral-flow device for diagnosis of invasive aspergillosis
    P Lewis White
    Public Health Wales, Microbiology Cardiff, UHW, Cardiff, United Kingdom
    J Clin Microbiol 51:1510-6. 2013
    ..An LFD assay targeting a novel specific biomarker has been developed, one which is methodologically simple and provides good clinical performance, particularly if combined with PCR...
  16. doi request reprint Comparison of four automated nucleic acid extraction platforms for the recovery of DNA from Aspergillus fumigatus
    Michael D Perry
    Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff, UK
    J Med Microbiol 63:1160-6. 2014
    ..The EZ1 and MagNA Pure platforms demonstrated equivalent high sensitivity and negative predictive values (97.4-100 %), essential for screening assays. ..
  17. pmc Evaluation of a commercially developed semiautomated PCR-surface-enhanced raman scattering assay for diagnosis of invasive fungal disease
    P Lewis White
    Public Health Wales, Microbiology Cardiff, UHW, Cardiff, United Kingdom
    J Clin Microbiol 52:3536-43. 2014
    ..This was a retrospective clinical evaluation, and performance is likely to be enhanced through a prospective analysis of clinical validity and by determining clinical utility...
  18. ncbi request reprint Isolation of Nucleic Acids for Fungal Diagnosis
    P Lewis White
    Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff, CF14 4XW, UK
    Methods Mol Biol 1508:223-247. 2017
    ..This chapter highlights benefits and limitations affecting the nucleic acid extraction process, before focusing on recent recommendations that through multicenter evaluation have provided optimal and standardized methodology...
  19. pmc Analytical and Clinical Evaluation of the PathoNostics AsperGenius Assay for Detection of Invasive Aspergillosis and Resistance to Azole Antifungal Drugs during Testing of Serum Samples
    P Lewis White
    Public Health Wales Microbiology Cardiff, Cardiff, United Kingdom
    J Clin Microbiol 53:2115-21. 2015
    ..While the available burden will limit application, it remains a significant advancement in the diagnosis and management of aspergillosis. ..
  20. doi request reprint The effect of sample storage on the performance and reproducibility of the galactomannan EIA test
    George Kimpton
    Infection, Immunity and Biochemistry, School of Medicine, Cardiff University, University Hospital of Wales, Cardiff, United Kingdom
    Med Mycol 52:618-26. 2014
    ..The freezing of samples prior to testing could potentially reduce false-positivity rates and the need to retest...
  21. pmc Potential for use of the Seegene Anyplex MTB/NTM real-time detection assay in a regional reference laboratory
    Michael D Perry
    Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff, United Kingdom
    J Clin Microbiol 52:1708-10. 2014
    ..00, 0.96, 0.93, and 1.00 for Mycobacterium tuberculosis complex (MTBC) and 1.00, 0.97, 0.75, and 1.00 for nontuberculous mycobacteria (NTM) detection, respectively, making it a suitable screening test for mycobacterial detection. ..