Tobias von der Haar
Affiliation: University of Kent
- The control of translational accuracy is a determinant of healthy ageing in yeastTobias von der Haar
Kent Fungal Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK
Open Biol 7:. 2017..Together, our findings indicate that signalling pathways regulating growth, protein homeostasis and energy metabolism may jointly safeguard accurate protein synthesis during healthy ageing...
- A quantitative estimation of the global translational activity in logarithmically growing yeast cellsTobias von der Haar
Protein Science Group, Department of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
BMC Syst Biol 2:87. 2008..Data on translational activity are difficult to generate experimentally under physiological conditions, however, translational activity as a parameter is in principle accessible through published genome-wide datasets...
- Optimized protein extraction for quantitative proteomics of yeastsTobias von der Haar
Protein Science Group, Department of Biosciences, University of Kent, Canterbury, United Kingdom
PLoS ONE 2:e1078. 2007..Current protocols for the extraction of proteins from yeast cells are likely to introduce artifacts into quantification procedures because of incomplete or selective extraction...
- Regulated translational bypass of stop codons in yeastTobias von der Haar
Protein Science Group, Department of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
Trends Microbiol 15:78-86. 2007..Rather than being a translation 'error', stop-codon readthrough can have important effects on other cellular processes such as mRNA degradation and, in some cases, can confer a beneficial phenotype to the cell...
- Folding transitions during assembly of the eukaryotic mRNA cap-binding complexTobias von der Haar
Manchester Interdisciplinary Biocentre, c o Jackson s Mill, University of Manchester, P O Box 88, Manchester M60 1QD, UK
J Mol Biol 356:982-92. 2006..We present structural, kinetic and mutagenesis data that allow us to deduce some of the detailed folding transitions that take place during the eIF4E interactions...
- Probing the role of structural features of mouse PrP in yeast by expression as Sup35-PrP fusionsLyne Jossé
Kent Fungal Group, School of Biosciences, University of Kent, Canterbury, UK
Prion 6:201-10. 2012..Through this study we illustrate the benefits and limitations of yeast-based models for protein misfolding disorders...
- Experimental and in silico modelling analyses of the gene expression pathway for recombinant antibody and by-product production in NS0 cell linesEmma J Mead
School of Biosciences, University of Kent, Canterbury, United Kingdom
PLoS ONE 7:e47422. 2012..The models also highlight cell line specific limitations in the antibody expression pathway...
- The mRNA cap-binding protein eIF4E in post-transcriptional gene expressionTobias von der Haar
Post transcriptional Control Group, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, PO Box 88, Manchester M60 1QD, UK
Nat Struct Mol Biol 11:503-11. 2004....
- Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeastGloria H Merritt
Kent Fungal Group and Protein Science Group, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
Nucleic Acids Res 38:5479-92. 2010..We reassess current models of stop-codon recognition by eRF1 in the light of these new data...
- Translation elongation can control translation initiation on eukaryotic mRNAsDominique Chu
School of Computing, University of Kent, Canterbury, UK
EMBO J 33:21-34. 2014..Codon usage thus evolved as a means to optimise translation on individual mRNAs, as well as global optimisation of ribosome availability. ..
- The architecture of eukaryotic translationDominique Chu
School of Computing, University of Kent, CT2 7NF Canterbury, UK
Nucleic Acids Res 40:10098-106. 2012..Furthermore, we find that strongly ribosome limited regimes are optimal for cells in that they are resource efficient and simplify the dynamics of the system...
- Development of a novel yeast cell-based system for studying the aggregation of Alzheimer's disease-associated Abeta peptides in vivoTobias von der Haar
Protein Science Group, Department of Biosciences, University of Kent, Canterbury, UK
Neurodegener Dis 4:136-47. 2007..We conclude that we have established a useful new tool for studying the aggregation of Abeta peptides in an organism in vivo...
- Control and regulation of mRNA translationEmma J Mead
Centre for Molecular Processing, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, U K
Biochem Soc Trans 42:151-4. 2014....
- A novel and versatile computational tool to model translationDominique Chu
School of Computing, University of Kent, Kent CT2 7NF, UK
Bioinformatics 28:292-3. 2012..The tool is written in Java, and is hence portable and can be parameterized for any organism...
- The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiaeDominique Chu
School of Computing, University of Kent, CT2 7NF, UK
Nucleic Acids Res 39:6705-14. 2011....
- Studying the assembly of multicomponent protein and ribonucleoprotein complexes using surface plasmon resonanceTobias von der Haar
Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, PO Box 88, Manchester M60 1QD, UK
Methods 29:167-74. 2003..Ways to experimentally avoid or, where this is not possible, to control for these complications are discussed...
- Synonymous codons, ribosome speed, and eukaryotic gene expression regulationDaniel Tarrant
Kent Fungal Group, School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK
Cell Mol Life Sci 71:4195-206. 2014..Here, we review the molecular mechanisms that determine ribosome speed in eukaryotic organisms, and discuss under which conditions ribosome speed can become the controlling parameter of gene expression levels. ..
- The translational machinery is an optimized molecular network that affects cellular homoeostasis and diseaseEleanna Kazana
Kent Fungal Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, U K
Biochem Soc Trans 42:173-6. 2014..How the eukaryotic translational machinery is optimized, and how it can maintain optimization in the face of changing internal parameters, are open questions relevant to the interaction between translation and cellular disease states. ..
- Identification of the limitations on recombinant gene expression in CHO cell lines with varying luciferase production ratesEmma J Mead
Department of Biosciences, University of Kent, Canterbury, UK
Biotechnol Bioeng 102:1593-602. 2009..The implications for the development of strategies to engineer cells for enhanced recombinant protein production levels are discussed...
- Biophysical studies of the translation initiation pathway with immobilized mRNA analogsJohn E G McCarthy
Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, United Kingdom
Methods Enzymol 430:247-64. 2007..In this chapter, we describe a number of approaches by which such immobilization can be achieved, followed by two specific examples for applications that use immobilized RNAs...
- tRNA and protein methylase complexes mediate zymocin toxicity in yeastPatrick Studte
Institut fur Biologie, Bereich Genetik, Martin Luther Universitat, Halle Wittenberg, Weinbergweg 10, D 06120 Halle Saale, Germany
Mol Microbiol 69:1266-77. 2008..Sup35, we observe that SUP45 overexpression and sup45 mutants suppress zymocin. Intriguingly, this suppression correlates with upregulated levels of tRNA species targeted by zymocin's tRNase activity...
- Ribosome loading onto the mRNA cap is driven by conformational coupling between eIF4G and eIF4EJohn D Gross
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
Cell 115:739-50. 2003..Our data explain how mRNA, eIF4E, and eIF4G exists as a stable mRNP that may facilitate multiple rounds of ribosomal loading during translation initiation, a key determinant in the overall rate of protein synthesis...
- The yeast eukaryotic initiation factor 4G (eIF4G) HEAT domain interacts with eIF1 and eIF5 and is involved in stringent AUG selectionHui He
Division of Biology, Kansas State University, Manhattan, KS 66506, USA
Mol Cell Biol 23:5431-45. 2003..These results strongly suggest that the binding of the eIF4G HEAT domain to eIF1 and eIF5 is important for maintaining the integrity of the scanning ribosomal preinitiation complex...
- Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex functionTobias von der Haar
Department of Biomolecular Sciences, Manchester, UK
Mol Microbiol 46:531-44. 2002..Overall, these data constitute an important basis for developing a quantitative model of the workings of the eukaryotic translation apparatus...
- Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complexRobert J C Gilbert
Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, United Kingdom
Proc Natl Acad Sci U S A 104:5788-93. 2007..This is expected to increase the accessibility of the mRNA channel, thus enabling the 40S subunit to convert to a recruitment-competent state...