Elizabeth Smythe

Summary

Affiliation: University of Sheffield
Country: UK

Publications

  1. ncbi request reprint Regulating the clathrin-coated vesicle cycle by AP2 subunit phosphorylation
    Elizabeth Smythe
    Division of Cell Biology and Immunology, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee, UK
    Trends Cell Biol 12:352-4. 2002
  2. doi request reprint Role of the rab5 Guanine nucleotide exchange factor, rme-6, in the regulation of clathrin-coated vesicle uncoating
    Elizabeth Smythe
    Department of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Western Bank, Florey Building, Room C09, Sheffield, S10 2TN, UK
    Methods Mol Biol 1298:283-93. 2015
  3. pmc The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton
    Elizabeth Smythe
    Centre for Developmental Genetics, Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, UK
    EMBO Rep 4:246-51. 2003
  4. pmc Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor micro2 kinase
    Antony P Jackson
    Department of Biomedical, University of Cambridge, Cambridge CB2 1TN, USA
    J Cell Biol 163:231-6. 2003
  5. doi request reprint Functions of actin in endocytosis
    Alastair S Robertson
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
    Cell Mol Life Sci 66:2049-65. 2009
  6. pmc Coordinated regulation of AP2 uncoating from clathrin-coated vesicles by rab5 and hRME-6
    Sophia Semerdjieva
    Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, England, UK
    J Cell Biol 183:499-511. 2008
  7. ncbi request reprint Actin regulation in endocytosis
    Elizabeth Smythe
    Department of Biomedical Science, Firth Court, Western Bank, University of Sheffield, Sheffield, UK
    J Cell Sci 119:4589-98. 2006
  8. doi request reprint Endocytosis of G protein-coupled receptors is regulated by clathrin light chain phosphorylation
    Filipe Ferreira
    Department of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield S10 2TN, UK
    Curr Biol 22:1361-70. 2012
  9. pmc TAG1 regulates the endocytic trafficking and signaling of the semaphorin3A receptor complex
    Puneet Dang
    Centre for Membrane Interactions and Dynamics, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Neurosci 32:10370-82. 2012
  10. ncbi request reprint Multiple roles for cyclin G-associated kinase in clathrin-mediated sorting events
    Claire X Zhang
    Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Traffic 6:1103-13. 2005

Collaborators

  • Kathryn R Ascough
  • Giampietro Schiavo
  • Eamonn Kelly
  • Barth Grant
  • D J Owen
  • Filipe Ferreira
  • Puneet Dang
  • Alastair S Robertson
  • Sophia Semerdjieva
  • Andrew Osborne
  • Claire X Zhang
  • Alexander Flett
  • Antony P Jackson
  • Alex Cooke
  • Graeme Henderson
  • Robert Woolley
  • Margaret Cunningham
  • Andrew J W Furley
  • Stuart Mundell
  • Gemma Smith
  • Matthew Foley
  • Kathryn R Ayscough
  • Paul Fonarev
  • Barry Shortt
  • Sukhdeep Singh
  • Emma Maxwell
  • Jonathan Hansen
  • Asa E Y Engqvist-Goldstein
  • David G Drubin
  • Sebastien Carreno
  • Frank R Wettey
  • Carl Smythe
  • Lindsay Hufton

Detail Information

Publications13

  1. ncbi request reprint Regulating the clathrin-coated vesicle cycle by AP2 subunit phosphorylation
    Elizabeth Smythe
    Division of Cell Biology and Immunology, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee, UK
    Trends Cell Biol 12:352-4. 2002
    ..Recent studies have provided particular insight into the role of phosphorylation of the cargo-binding micro2 subunit of AP2...
  2. doi request reprint Role of the rab5 Guanine nucleotide exchange factor, rme-6, in the regulation of clathrin-coated vesicle uncoating
    Elizabeth Smythe
    Department of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Western Bank, Florey Building, Room C09, Sheffield, S10 2TN, UK
    Methods Mol Biol 1298:283-93. 2015
    ..This assay has been used to investigate rab5-dependent modulation of uncoating. Specifically, it has been used to identify functional differences between rab5 guanine nucleotide exchange factors. ..
  3. pmc The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton
    Elizabeth Smythe
    Centre for Developmental Genetics, Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, UK
    EMBO Rep 4:246-51. 2003
    ....
  4. pmc Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor micro2 kinase
    Antony P Jackson
    Department of Biomedical, University of Cambridge, Cambridge CB2 1TN, USA
    J Cell Biol 163:231-6. 2003
    ..Our results imply a central role for clathrin in the regulation of cargo selection via the modulation of phospho-mu2 levels...
  5. doi request reprint Functions of actin in endocytosis
    Alastair S Robertson
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
    Cell Mol Life Sci 66:2049-65. 2009
    ..Our discussion is limited to endocytosis involving clathrin as other types of endocytosis have not been demonstrated in yeast...
  6. pmc Coordinated regulation of AP2 uncoating from clathrin-coated vesicles by rab5 and hRME-6
    Sophia Semerdjieva
    Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, England, UK
    J Cell Biol 183:499-511. 2008
    ..These data are consistent with a model in which hRME-6 and rab5 regulate AP2 uncoating in vivo by coordinately regulating mu2 dephosphorylation and PtdIns(4,5)P(2) levels in CCVs...
  7. ncbi request reprint Actin regulation in endocytosis
    Elizabeth Smythe
    Department of Biomedical Science, Firth Court, Western Bank, University of Sheffield, Sheffield, UK
    J Cell Sci 119:4589-98. 2006
    ..Furthermore, live cell imaging indicates that spatiotemporal aspects of actin recruitment and vesicle formation are likely to be conserved across eukaryotic evolution...
  8. doi request reprint Endocytosis of G protein-coupled receptors is regulated by clathrin light chain phosphorylation
    Filipe Ferreira
    Department of Biomedical Science, Centre for Membrane Interactions and Dynamics, University of Sheffield, Sheffield S10 2TN, UK
    Curr Biol 22:1361-70. 2012
    ..Despite the identification of clathrin-coated pits at the cell surface over 30 years ago, the functions of CLCs in endocytosis have been elusive...
  9. pmc TAG1 regulates the endocytic trafficking and signaling of the semaphorin3A receptor complex
    Puneet Dang
    Centre for Membrane Interactions and Dynamics, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Neurosci 32:10370-82. 2012
    ..These observations identify a novel role for TAG1 in modulating the intracellular sorting of signaling receptor complexes...
  10. ncbi request reprint Multiple roles for cyclin G-associated kinase in clathrin-mediated sorting events
    Claire X Zhang
    Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Traffic 6:1103-13. 2005
    ..Only GAK with a functional J domain could rescue the defect, suggesting that GAK is important for clathrin uncoating. Furthermore, we demonstrated that GAK plays a role in the clathrin-dependent trafficking from the trans Golgi network...
  11. doi request reprint Endocytosis assays in intact and permeabilized cells
    Andrew Osborne
    Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Cell Biol . 2005
    ..Reconstitution assays in permeabilized cells provide a powerful approach to understanding how this complex process is regulated...
  12. ncbi request reprint Cell biology: light on pits
    Elizabeth Smythe
    Nature 431:641-2. 2004
  13. ncbi request reprint Direct interactions between rab GTPases and cargo
    Elizabeth Smythe
    School of Life Sciences, Division of Cell Biology and Immunology, Wellcome Trust Biocentre, University of Dundee, Dundee, United Kingdom
    Mol Cell 9:205-6. 2002
    ..New data show that cargo proteins are also rab effectors and can therefore regulate their own trafficking by direct interactions with the transport machinery...