Carol V Robinson

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi request reprint Watching and weighting--chaperone complexes in action
    Carol V Robinson
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    Nat Methods 2:331-2. 2005
  2. ncbi request reprint Protein complexes take flight
    Carol V Robinson
    Mass Spectrometry, University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    Nat Struct Biol 9:505-6. 2002
  3. ncbi request reprint The molecular sociology of the cell
    Carol V Robinson
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Nature 450:973-82. 2007
  4. ncbi request reprint The co-chaperone p23 arrests the Hsp90 ATPase cycle to trap client proteins
    Stephen H McLaughlin
    Cambridge University, Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 356:746-58. 2006
  5. ncbi request reprint "Zn-link": a metal-sharing interface that organizes the quaternary structure and catalytic site of the endoribonuclease, RNase E
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    Biochemistry 44:4667-75. 2005
  6. pmc A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation
    Erwin De Genst
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U K
    J Phys Chem B 117:13245-58. 2013
  7. doi request reprint Subunit architecture of intact protein complexes from mass spectrometry and homology modeling
    Thomas Taverner
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Acc Chem Res 41:617-27. 2008
  8. ncbi request reprint Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 340:965-79. 2004
  9. pmc Quaternary dynamics and plasticity underlie small heat shock protein chaperone function
    Florian Stengel
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    Proc Natl Acad Sci U S A 107:2007-12. 2010
  10. pmc Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding
    Min Zhou
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford OX1 3QZ, UK
    Science 334:380-5. 2011

Collaborators

Detail Information

Publications120 found, 100 shown here

  1. ncbi request reprint Watching and weighting--chaperone complexes in action
    Carol V Robinson
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    Nat Methods 2:331-2. 2005
  2. ncbi request reprint Protein complexes take flight
    Carol V Robinson
    Mass Spectrometry, University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    Nat Struct Biol 9:505-6. 2002
  3. ncbi request reprint The molecular sociology of the cell
    Carol V Robinson
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Nature 450:973-82. 2007
    ..With these structures in hand, researchers will be poised to use cryo-electron tomography to view protein complexes in action within cells, providing unprecedented insights into protein-interaction networks...
  4. ncbi request reprint The co-chaperone p23 arrests the Hsp90 ATPase cycle to trap client proteins
    Stephen H McLaughlin
    Cambridge University, Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 356:746-58. 2006
    ..We propose a model where the role of p23 is to lock individual subunits of Hsp90 in an ATP-dependent conformational state that has a high affinity for client proteins...
  5. ncbi request reprint "Zn-link": a metal-sharing interface that organizes the quaternary structure and catalytic site of the endoribonuclease, RNase E
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    Biochemistry 44:4667-75. 2005
    ..One or both interfaces organize the active site, which is distinct from the primary site of RNA binding...
  6. pmc A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation
    Erwin De Genst
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U K
    J Phys Chem B 117:13245-58. 2013
    ....
  7. doi request reprint Subunit architecture of intact protein complexes from mass spectrometry and homology modeling
    Thomas Taverner
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Acc Chem Res 41:617-27. 2008
    ..Overall therefore this mass spectrometry and homology modeling approach has given significant insight into the structure of two previously intractable protein complexes and as such has broad application in structural biology...
  8. ncbi request reprint Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 340:965-79. 2004
    ..The carboxy-terminal domain of RNase E may thus act as a flexible tether of the degradosome components. The implications of these and other observations for the organization of the RNA degradosome are discussed...
  9. pmc Quaternary dynamics and plasticity underlie small heat shock protein chaperone function
    Florian Stengel
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    Proc Natl Acad Sci U S A 107:2007-12. 2010
    ..This extends the paradigm that intrinsic dynamics are crucial to protein function to include equilibrium fluctuations in quaternary structure, and suggests they are integral to the sHSPs' role in the cellular protein homeostasis network...
  10. pmc Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding
    Min Zhou
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford OX1 3QZ, UK
    Science 334:380-5. 2011
    ..Consequently, we can link specific lipid and nucleotide binding with distinct regulatory roles...
  11. pmc Characterization of symmetric complexes of nerve growth factor and the ectodomain of the pan-neurotrophin receptor, p75NTR
    Jukka P Aurikko
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    J Biol Chem 280:33453-60. 2005
    ..Molecular models of the 2:2 p75(NTR) x NGF complex based on these data are not consistent with the further assembly of either symmetric (2:2:2) or asymmetric (2:2:1) ternary p75(NTR) x NGF x TrkA complexes...
  12. ncbi request reprint Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris
    Janet R Kumita
    Department of Chemistry, University of Cambridge, UK
    FEBS J 273:711-20. 2006
    ..These findings indicate that a complex interplay between reduced native-state stability, lower secretion levels, and protein aggregation propensity influences the types of mutation that give rise to familial forms of amyloid disease...
  13. doi request reprint Micelles protect membrane complexes from solution to vacuum
    Nelson P Barrera
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK
    Science 321:243-6. 2008
    ....
  14. pmc A non-natural variant of human lysozyme (I59T) mimics the in vitro behaviour of the I56T variant that is responsible for a form of familial amyloidosis
    Christine L Hagan
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Protein Eng Des Sel 23:499-506. 2010
    ..Moreover, we demonstrate that the I59T variant can readily form fibrils in vitro, similar in nature to those of the amyloidogenic I56T variant, under significantly milder conditions than are needed for the wild-type protein...
  15. pmc Small heat shock protein activity is regulated by variable oligomeric substructure
    Justin L P Benesch
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
    J Biol Chem 283:28513-7. 2008
    ..We propose that a bias toward monomeric substructure is responsible for the aberrant chaperone behavior associated with the alpha-crystallins in protein deposition diseases...
  16. ncbi request reprint Subunit exchange of polydisperse proteins: mass spectrometry reveals consequences of alphaA-crystallin truncation
    J Andrew Aquilina
    Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, United Kingdom
    J Biol Chem 280:14485-91. 2005
    ....
  17. ncbi request reprint L55P transthyretin accelerates subunit exchange and leads to rapid formation of hybrid tetramers
    Catherine A Keetch
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
    J Biol Chem 280:41667-74. 2005
    ..This implies interactions between the two proteins that accelerate the formation of hybrid tetramers, a result with important implications for transthyretin amyloidosis...
  18. ncbi request reprint Tandem mass spectrometry reveals the quaternary organization of macromolecular assemblies
    Justin L P Benesch
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom
    Chem Biol 13:597-605. 2006
    ..Using this basis of understanding, we highlight differences in the dissociation pathways of three related macromolecular assemblies and show how these are a direct consequence of changes in both local and global oligomeric organization...
  19. pmc Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria
    Leopold L Ilag
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    Proc Natl Acad Sci U S A 102:8192-7. 2005
    ....
  20. ncbi request reprint Reduced global cooperativity is a common feature underlying the amyloidogenicity of pathogenic lysozyme mutations
    Mireille Dumoulin
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 346:773-88. 2005
    ....
  21. ncbi request reprint Biochemical and structural studies of the interaction of Cdc37 with Hsp90
    Wei Zhang
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 340:891-907. 2004
    ....
  22. pmc Isoforms of U1-70k control subunit dynamics in the human spliceosomal U1 snRNP
    Helena Hernandez
    Department of Chemistry, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 4:e7202. 2009
    ..These results therefore provide the important functional link between proteomics and structure as well as insight into the dynamic quaternary structure of the native U1 snRNP important for its function...
  23. pmc Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site
    Victor M Bolanos-Garcia
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Structure 19:1691-700. 2011
    ..We also show that substitution of several BUBR1 residues engaged in binding Blinkin leads to defects in the SAC, thus providing the first molecular details of the recognition mechanism underlying kinetochore-SAC signaling...
  24. pmc Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes
    Argyris Politis
    Department of Chemistry, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e12080. 2010
    ....
  25. ncbi request reprint The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species
    Janet R Kumita
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK
    J Mol Biol 369:157-67. 2007
    ....
  26. pmc Polypyrimidine tract-binding protein stimulates the poliovirus IRES by modulating eIF4G binding
    Panagiota Kafasla
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    EMBO J 29:3710-22. 2010
    ....
  27. ncbi request reprint Rationalising lysozyme amyloidosis: insights from the structure and solution dynamics of T70N lysozyme
    Russell J K Johnson
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 352:823-36. 2005
    ....
  28. ncbi request reprint Me, my cell, and I: the role of the collision cell in the tandem mass spectrometry of macromolecules
    Margaret G McCammon
    Cambridge University Chemical Laboratory, Cambridge University, Cambridge, UK
    Biotechniques 39:447, 449, 451 passim. 2005
  29. doi request reprint Acetylation of L12 increases interactions in the Escherichia coli ribosomal stalk complex
    Yuliya Gordiyenko
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 380:404-14. 2008
    ..5 MDa particle...
  30. ncbi request reprint Conformational dynamics of the molecular chaperone Hsp90 in complexes with a co-chaperone and anticancer drugs
    Jonathan J Phillips
    Cambridge University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK Department of Biochemistry, University of Cambridge, Old Addenbrookes Site, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
    J Mol Biol 372:1189-203. 2007
    ..For the inhibitors, differences in the interface between the middle domain and the C-terminal domain were also observed. These data provide important insight into the structure of the biologically active form of the protein...
  31. ncbi request reprint Mass spectrometry reveals the missing links in the assembly pathway of the bacterial 20 S proteasome
    Michal Sharon
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom
    J Biol Chem 282:18448-57. 2007
    ..In summary, this study provides evidence for transient intermediates in the assembly pathway and reveals detailed insight into the cleavage sites of the propeptide...
  32. pmc Detergent-free mass spectrometry of membrane protein complexes
    Jonathan T S Hopper
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK
    Nat Methods 10:1206-8. 2013
    ..We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent. ..
  33. doi request reprint Engineering a camelid antibody fragment that binds to the active site of human lysozyme and inhibits its conversion into amyloid fibrils
    Pak Ho Chan
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Biochemistry 47:11041-54. 2008
    ..Moreover, this work further demonstrates the unique properties of camelid single-domain antibody fragments as structural probes for studying the mechanism of aggregation and as potential inhibitors of fibril formation...
  34. pmc The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome
    Maria W Górna
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    RNA 16:553-62. 2010
    ..The multidentate interactions of RraA impede the RNA-binding and ribonuclease activities of the degradosome and may result in complex modulation and rerouting of degradosome activity...
  35. pmc The quaternary organization and dynamics of the molecular chaperone HSP26 are thermally regulated
    Justin L P Benesch
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford, UK
    Chem Biol 17:1008-17. 2010
    ..This is consistent with a conformational change of the oligomers at elevated temperatures which regulates the disassembly rates of this thermally activated protein...
  36. pmc Mass spectrometry defines the stoichiometry of ribosomal stalk complexes across the phylogenetic tree
    Yuliya Gordiyenko
    Department of Chemistry, University of Cambridge, Cambridge, United Kingdom
    Mol Cell Proteomics 9:1774-83. 2010
    ..Moreover the ratio of pentameric to heptameric stalk complexes changed during the course of cell growth. We consider these differences in stoichiometry within ribosomal stalk complexes in the context of convergent evolution...
  37. doi request reprint Gas-phase unfolding and disassembly reveals stability differences in ligand-bound multiprotein complexes
    Suk Joon Hyung
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Chem Biol 16:382-90. 2009
    ..Overall, therefore, our results have implications for small-molecule drug design directed at multiprotein targets...
  38. pmc Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters
    Michael Landreh
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, Oxfordshire OX1 3QZ, UK
    Nat Commun 8:13993. 2017
    ..We speculate that elevator-type antiporters such as NapA, and likely NHA2, use a subset of annular lipids as structural support to facilitate large-scale conformational changes within the membrane...
  39. ncbi request reprint Evidence for macromolecular protein rings in the absence of bulk water
    Brandon T Ruotolo
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Science 310:1658-61. 2005
    ....
  40. pmc Subunit architecture of multimeric complexes isolated directly from cells
    Helena Hernandez
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    EMBO Rep 7:605-10. 2006
    ....
  41. ncbi request reprint The component polypeptide chains of bovine insulin nucleate or inhibit aggregation of the parent protein in a conformation-dependent manner
    Glyn L Devlin
    Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK
    J Mol Biol 360:497-509. 2006
    ....
  42. doi request reprint Ion mobility mass spectrometry of two tetrameric membrane protein complexes reveals compact structures and differences in stability and packing
    Sheila C Wang
    Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK
    J Am Chem Soc 132:15468-70. 2010
    ..These results highlight the potential of ion mobility mass spectrometry for characterizing the overall topologies of membrane protein complexes and the structural changes associated with nucleotide, lipid, and drug binding...
  43. doi request reprint The role of interfacial lipids in stabilizing membrane protein oligomers
    Kallol Gupta
    Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK
    Nature . 2017
    ....
  44. pmc A single subunit directs the assembly of the Escherichia coli DNA sliding clamp loader
    Ah Young Park
    Department of Chemistry, University of Oxford, Oxford, UK
    Structure 18:285-92. 2010
    ..The implications for intracellular assembly of the DNA polymerase III holoenzyme are discussed...
  45. pmc Comparative cross-linking and mass spectrometry of an intact F-type ATPase suggest a role for phosphorylation
    Carla Schmidt
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford OX1 3QZ, UK
    Nat Commun 4:1985. 2013
    ....
  46. doi request reprint Separating and visualising protein assemblies by means of preparative mass spectrometry and microscopy
    Justin L P Benesch
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Struct Biol 172:161-8. 2010
    ....
  47. pmc An oligomeric signaling platform formed by the Toll-like receptor signal transducers MyD88 and IRAK-4
    Precious G Motshwene
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    J Biol Chem 284:25404-11. 2009
    ..Taken together, these findings indicate that TLR activation causes the formation of a highly oligomeric signaling platform analogous to the death-inducing signaling complex of the Fas receptor pathway...
  48. pmc Multimers of the fibroblast growth factor (FGF)-FGF receptor-saccharide complex are formed on long oligomers of heparin
    Nicholas J Harmer
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Biochem J 393:741-8. 2006
    ....
  49. doi request reprint Correlating solution binding and ESI-MS stabilities by incorporating solvation effects in a confined cucurbit[8]uril system
    Urs Rauwald
    Melville Laboratory for Polymer Synthesis, and Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Phys Chem B 114:8606-15. 2010
    ..Finally, this treatment enabled us to predict association constants that were in excellent agreement with measured values, including several highly insoluble guest compounds...
  50. doi request reprint Ion mobility-mass spectrometry reveals the influence of subunit packing and charge on the dissociation of multiprotein complexes
    Elisabetta Boeri Erba
    University Chemistry Department, University of Cambridge, Cambridge, United Kingdom
    Anal Chem 82:9702-10. 2010
    ..This investigation increases our understanding of the factors governing the CID of protein complexes and moves us closer to the goal of obtaining structural information on subunit interactions and packing from gas-phase experiments...
  51. ncbi request reprint Mass measurements of increased accuracy resolve heterogeneous populations of intact ribosomes
    Adam R McKay
    Department of Chemistry, University of Cambridge, UK
    J Am Chem Soc 128:11433-42. 2006
    ..More generally, the results establish a rigorous procedure for accurate mass measurement and spectral analysis of heterogeneous macromolecular assemblies...
  52. pmc The N-terminal region of the human autophagy protein ATG16L1 contains a domain that folds into a helical structure consistent with formation of a coiled-coil
    Rhiannon Parkhouse
    Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 8:e76237. 2013
    ..We also observe extensive sequence conservation across vertebrates providing strong support for the crucial functional role of the ATG16L1 coiled-coil. ..
  53. pmc Heteronuclear NMR investigations of dynamic regions of intact Escherichia coli ribosomes
    John Christodoulou
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    Proc Natl Acad Sci U S A 101:10949-54. 2004
    ....
  54. doi request reprint Charge-state dependent compaction and dissociation of protein complexes: insights from ion mobility and molecular dynamics
    Zoe Hall
    Department of Chemistry, University of Oxford, Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford OX1 3QZ, United Kingdom
    J Am Chem Soc 134:3429-38. 2012
    ..This strong correlation between theory and experiment has implications for further studies as well as for understanding the process of CID and for applications to gas-phase structural biology more generally...
  55. ncbi request reprint Quaternary structure and catalytic activity of the Escherichia coli ribonuclease E amino-terminal catalytic domain
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Biochemistry 42:13848-55. 2003
    ..These observations have implications for the structure and function of the RNase E/RNase G ribonuclease family and for the assembly of the E. coli RNA degradosome, in which RNase E is the central component...
  56. pmc Hsp70 oligomerization is mediated by an interaction between the interdomain linker and the substrate-binding domain
    Francesco A Aprile
    Department of Chemistry, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 8:e67961. 2013
    ..Taken together, these findings suggest a possible role of chaperone oligomerization as a mechanism for regulating the availability of the active monomeric form of the chaperone and for the control of substrate binding and release. ..
  57. pmc Phospholipid complexation and association with apolipoprotein C-II: insights from mass spectrometry
    Charlotte L Hanson
    University Chemistry Department, University of Cambridge, Cambridge CB2 1EW, UK
    Biophys J 85:3802-12. 2003
    ....
  58. pmc Heterogeneity and dynamics in the assembly of the heat shock protein 90 chaperone complexes
    Ima Obong Ebong
    Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3TA, United Kingdom
    Proc Natl Acad Sci U S A 108:17939-44. 2011
    ..In this case, the K(D) values afford insights into the assembly of ten Hsp90-containing complexes and provide a rationale for the cellular heterogeneity and prevalence of intermediates in the Hsp90 chaperone cycle...
  59. pmc Mass spectrometry--from peripheral proteins to membrane motors
    Nina Morgner
    Department of Chemistry, University of Oxford, South Parks Road, Oxford, OX1 5QY, UK
    J Mol Biol 423:1-13. 2012
    ..In this perspective, we track these developments and suggest explanations for the various discoveries made during this research...
  60. ncbi request reprint 20S proteasomes have the potential to keep substrates in store for continual degradation
    Michal Sharon
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    J Biol Chem 281:9569-75. 2006
    ....
  61. pmc Structural organization of the 19S proteasome lid: insights from MS of intact complexes
    Michal Sharon
    Department of Chemistry, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 4:e267. 2006
    ..More generally, the results highlight the potential of mass spectrometry to add crucial insight into the structural organization of an endogenous, wild-type complex...
  62. ncbi request reprint Stoichiometry and localization of the stator subunits E and G in Thermus thermophilus H+-ATPase/synthase
    Olga Esteban
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge, United Kingdom
    J Biol Chem 283:2595-603. 2008
    ..Both approaches could be applicable to the structural analysis of other membrane protein complexes...
  63. pmc Structural basis for the inhibition of activin signalling by follistatin
    Adrian E Harrington
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    EMBO J 25:1035-45. 2006
    ..The structure provides a detailed description of the inhibitory mechanism and gives insights into interactions of follistatin with other TGF-beta family proteins...
  64. ncbi request reprint The flight of macromolecular complexes in a mass spectrometer
    Frank Sobott
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Philos Transact A Math Phys Eng Sci 363:379-89; discussion 389-91. 2005
    ..Overall, therefore, we show that mass spectrometry used in this way is capable of addressing features of the energy landscape not readily accessed by traditional structural biology approaches...
  65. ncbi request reprint Ion mobility-mass spectrometry reveals long-lived, unfolded intermediates in the dissociation of protein complexes
    Brandon T Ruotolo
    Cambridge University, Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW, UK
    Angew Chem Int Ed Engl 46:8001-4. 2007
  66. ncbi request reprint Phosphorylation of alphaB-crystallin alters chaperone function through loss of dimeric substructure
    J Andrew Aquilina
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
    J Biol Chem 279:28675-80. 2004
    ....
  67. doi request reprint Intrinsically disordered p53 and its complexes populate compact conformations in the gas phase
    Kevin Pagel
    Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, UK
    Angew Chem Int Ed Engl 52:361-5. 2013
    ..When intrinsically disordered segments are introduced into the protein sequence, however, the complex spontaneously collapses in the gas phase to a compact conformation...
  68. pmc Mass spectrometry reveals differences in stability and subunit interactions between activated and nonactivated conformers of the (αβγδ)4 phosphorylase kinase complex
    Laura A Lane
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Mol Cell Proteomics 11:1768-76. 2012
    ....
  69. doi request reprint Do charge state signatures guarantee protein conformations?
    Zoe Hall
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK
    J Am Soc Mass Spectrom 23:1161-8. 2012
    ..The exact nature of the side-chain packing and secondary structural elements in these compact states, however, remains elusive and prompts further research...
  70. doi request reprint Characterisation of serpin polymers in vitro and in vivo
    Didier Belorgey
    Dept of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Methods 53:255-66. 2011
    ..The characterisation of the pathological polymers requires heating of the folded protein or alternatively the assessment of ordered polymers from cell and animal models of disease or from the tissues of humans who carry the mutation...
  71. ncbi request reprint Protein complexes in the gas phase: technology for structural genomics and proteomics
    Justin L P Benesch
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Chem Rev 107:3544-67. 2007
  72. doi request reprint Non-homologous end-joining partners in a helical dance: structural studies of XLF-XRCC4 interactions
    Qian Wu
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
    Biochem Soc Trans 39:1387-92, suppl 2 p following 1392. 2011
    ....
  73. pmc The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores
    Victor M Bolanos-Garcia
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Structure 17:105-16. 2009
    ..The structure provides insight into the molecular basis of Blinkin-specific recognition by BUB1 and, on a broader perspective, of the mechanism that mediates kinetochore localization of BUB1 in checkpoint-activated cells...
  74. ncbi request reprint Site-specific hydrogen exchange of proteins: insights into the structures of amyloidogenic intermediates
    Zhong ping Yao
    Department of Chemistry, University of Cambridge, UK
    Methods Enzymol 402:389-402. 2005
    ....
  75. ncbi request reprint The role of mass spectrometry in structure elucidation of dynamic protein complexes
    Michal Sharon
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
    Annu Rev Biochem 76:167-93. 2007
    ..Moreover, recent successes in revealing the overall subunit architecture of complexes are set to promote MS from a complementary approach to a structural biology tool in its own right...
  76. ncbi request reprint Mass spectrometry of intact ribosomes
    Hortense Videler
    The University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, UK
    FEBS Lett 579:943-7. 2005
    ..We also describe how improvements in technology and understanding have widened the scope of our research and lead to dramatic improvements in quality and information available from spectra of intact ribosomes...
  77. doi request reprint Structural insight into the mechanism of activation of the Toll receptor by the dimeric ligand Spätzle
    Monique Gangloff
    Department of Biochemistry, Cambridge University, Cambridge, UK
    J Biol Chem 283:14629-35. 2008
    ..The unexpected 2:2 complex was confirmed by mass spectrometry under native conditions. These results suggest that activation of Toll is an allosteric mechanism induced by an end-on binding mode of its ligand Spz...
  78. doi request reprint Alternate dissociation pathways identified in charge-reduced protein complex ions
    Kevin Pagel
    University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW, UK
    Anal Chem 82:5363-72. 2010
    ..Taken together, the results presented highlight the potential of charge state modulation as a method for directing the course of gas-phase dissociation and unfolding of protein complexes...
  79. ncbi request reprint Evidence for micellar structure in the gas phase
    Michal Sharon
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Am Chem Soc 129:8740-6. 2007
    ..Overall, therefore, our results show that the solution-phase orientation of surfactants is preserved during electrospray and are consistent with interactions being maintained between surfactants and an encapsulated protein...
  80. doi request reprint Quadrupole-time-of-flight mass spectrometer modified for higher-energy dissociation reduces protein assemblies to peptide fragments
    Justin L P Benesch
    Department of Chemistry, University of Cambridge, UK
    Anal Chem 81:1270-4. 2009
    ....
  81. ncbi request reprint Mass spectrometry of ribosomes from Saccharomyces cerevisiae: implications for assembly of the stalk complex
    Charlotte L Hanson
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    J Biol Chem 279:42750-7. 2004
    ..This implies that assembly of the stalk complex is not governed solely by interactions with P0 but is a cooperative process involving binding to partner proteins for additional stability on the ribosome...
  82. ncbi request reprint Molecular recycling within amyloid fibrils
    Natàlia Carulla
    University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge CB2 1EW, UK
    Nature 436:554-8. 2005
    ..This insight into the dynamic nature of amyloid fibrils, and the ability to determine the parameters that define this behaviour, have important implications for the design of therapeutic strategies directed against amyloid disease...
  83. pmc Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions
    Nelson P Barrera
    Department of Chemistry, University of Cambridge, Cambridge, UK
    Nat Methods 6:585-7. 2009
    ..This enabled us to characterize both subunit stoichiometry and lipid binding in 4 membrane protein complexes...
  84. ncbi request reprint Activation of ubiquitin ligase SCF(Skp2) by Cks1: insights from hydrogen exchange mass spectrometry
    Zhong ping Yao
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 363:673-86. 2006
    ....
  85. doi request reprint Real-time monitoring of protein complexes reveals their quaternary organization and dynamics
    Alexander J Painter
    Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, United Kingdom
    Chem Biol 15:246-53. 2008
    ..Furthermore, we propose that the approach used here is applicable to a range of nonequilibrium systems and is capable of providing both structural and dynamical information necessary for functional genomics...
  86. ncbi request reprint Metal-dependent folding and stability of nuclear hormone receptor DNA-binding domains
    Lieh Yoon Low
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 319:87-106. 2002
    ..The comparatively compact shapes of the denatured and apo forms may explain, in part, the marginal stability of the native fold...
  87. ncbi request reprint Towards a resolution of the stoichiometry of the fibroblast growth factor (FGF)-FGF receptor-heparin complex
    Nicholas J Harmer
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 339:821-34. 2004
    ....
  88. doi request reprint Structures of SAS-6 suggest its organization in centrioles
    Mark van Breugel
    Medical Research Council Laboratory of Molecular Biology MRC LMB, Hills Road, Cambridge, UK
    Science 331:1196-9. 2011
    ..Thus, these interactions are probably essential to the structural organization of cartwheel centers...
  89. doi request reprint Ion mobility-mass spectrometry analysis of large protein complexes
    Brandon T Ruotolo
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Nat Protoc 3:1139-52. 2008
    ..The protocol also covers basic instrument settings and best practices for both observation and detection of large noncovalent protein complexes by ion mobility-mass spectrometry...
  90. pmc Assembly reflects evolution of protein complexes
    Emmanuel D Levy
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 453:1262-5. 2008
    ..Our model of self-assembly allows reliable prediction of evolution and assembly of a complex solely from its crystal structure...
  91. pmc Observation of sequence specificity in the seeding of protein amyloid fibrils
    Mark R H Krebs
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Protein Sci 13:1933-8. 2004
    ..In addition, it is consistent with the observation of a single dominant type of protein in the deposits associated with each form of amyloid disease...
  92. ncbi request reprint Protein complexes gain momentum
    Frank Sobott
    Cambridge University Chemical Laboratory, Lensfield Road, UK
    Curr Opin Struct Biol 12:729-34. 2002
    ....
  93. ncbi request reprint Mass spectrometry of macromolecular assemblies: preservation and dissociation
    Justin L P Benesch
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Curr Opin Struct Biol 16:245-51. 2006
    ....
  94. ncbi request reprint A camelid antibody fragment inhibits the formation of amyloid fibrils by human lysozyme
    Mireille Dumoulin
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Nature 424:783-8. 2003
    ....
  95. ncbi request reprint Aspects of native proteins are retained in vacuum
    Brandon T Ruotolo
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK
    Curr Opin Chem Biol 10:402-8. 2006
    ....
  96. ncbi request reprint Subunit exchange of multimeric protein complexes. Real-time monitoring of subunit exchange between small heat shock proteins by using electrospray mass spectrometry
    Frank Sobott
    Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    J Biol Chem 277:38921-9. 2002
    ..More generally, these experiments illustrate a powerful approach for the real-time analysis of the evolution of transient species and their relative populations during the subunit exchange of multimeric protein complexes...
  97. ncbi request reprint Structural change in response to ligand binding
    Margaret G McCammon
    Cambridge University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, UK
    Curr Opin Chem Biol 8:60-5. 2004
    ..In this article we review recent applications of MS for selection of ligands and definition of their binding characteristics for individual protein targets through to macromolecular complexes such as ribosomes...
  98. pmc Polydispersity of a mammalian chaperone: mass spectrometry reveals the population of oligomers in alphaB-crystallin
    J Andrew Aquilina
    Department of Chemistry, Cambridge University, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    Proc Natl Acad Sci U S A 100:10611-6. 2003
    ....
  99. doi request reprint When proteomics meets structural biology
    Min Zhou
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, UK
    Trends Biochem Sci 35:522-9. 2010
    ..In this review, we discuss how superimposing proteomics information onto both low-resolution 3D models and high-resolution atomic structures can reveal new insight into function...
  100. ncbi request reprint Thermal dissociation of multimeric protein complexes by using nanoelectrospray mass spectrometry
    Justin L P Benesch
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    Anal Chem 75:2208-14. 2003
    ..As such, this probe allows the study of the reversible heat-induced changes of noncovalent complexes in a biologically relevant manner...
  101. ncbi request reprint Drug binding revealed by tandem mass spectrometry of a protein-micelle complex
    Leopold L Ilag
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Am Chem Soc 126:14362-3. 2004
    ..Taken together, the results provide direct evidence for drug binding within a relevant gas-phase protein-micelle complex...