Matthew D W Piper
Affiliation: University College London
- Diet, metabolism and lifespan in DrosophilaMatthew D W Piper
UCL Centre for Research on Ageing, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
Exp Gerontol 40:857-62. 2005..Current data indicate lifespan-extension by DR is likely to involve both enhancement of various defense and detoxification mechanisms and a complex range of metabolic alterations that make energy available for these processes...
- Calories do not explain extension of life span by dietary restriction in DrosophilaWilliam Mair
Department of Biology, Centre for Research on Ageing, University College London, London, United Kingdom
PLoS Biol 3:e223. 2005..Calorie intake is therefore not the key factor in the reduction of mortality rate by DR in this species...
- Evolutionary conservation of regulated longevity assurance mechanismsJoshua J McElwee
Department of Biology, University College London, London WC1E 6BT, UK
Genome Biol 8:R132. 2007..To address this, we have applied a novel, multi-level cross-species comparative analysis to compare gene expression changes accompanying increased longevity in mutant nematodes, fruitflies and mice with reduced IIS...
- Dietary restriction and aging: a unifying perspectiveMatthew D W Piper
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, UK
Cell Metab 14:154-60. 2011..We provide examples showing that it is more informative to analyze DR in terms of dietary balance and that dietary optimization for life span is critical for studies examining the biology of aging and other traits...
- Diet and agingMatthew D W Piper
UCL Institute of Healthy Ageing, University College London, London, WC1E 6BT, UK
Cell Metab 8:99-104. 2008..Here we discuss examples of diet/genotype interactions that show a more complex mechanistic view is required and that mild dietary modifications can dramatically change the interpretation of model organism aging studies...
- A holidic medium for Drosophila melanogasterMatthew D W Piper
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK
Nat Methods 11:100-5. 2014..We used this holidic medium to investigate amino acid-specific effects on food-choice behavior and report that folic acid from the microbiota is sufficient for Drosophila development. ..
- Effect of a standardised dietary restriction protocol on multiple laboratory strains of Drosophila melanogasterRichard C Grandison
UCL Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK
PLoS ONE 4:e4067. 2009..Outcomes of lifespan studies in model organisms are particularly susceptible to variations in technical procedures. This is especially true of dietary restriction, which is implemented in many different ways among laboratories...
- Optimization of dietary restriction protocols in DrosophilaTimothy M Bass
UCL Centre for Research on Ageing, Department of Biology, University College London, Gower St, London, WC1E 6BT, UK
J Gerontol A Biol Sci Med Sci 62:1071-81. 2007..We propose procedures to ensure that diets are suitable for the study of DR in Drosophila...
- Counting the calories: the role of specific nutrients in extension of life span by food restrictionMatthew D W Piper
Department of Biology, University College London, Darwin Building, Gower Street, London, WC1E 6BT, UK
J Gerontol A Biol Sci Med Sci 60:549-55. 2005..Delineation of the nutritional components that are critical for life-span extension will help to reveal the mechanisms by which it operates...
- Pitfalls of measuring feeding rate in the fruit fly Drosophila melanogasterRichard Wong
Institute for Healthy Ageing, Darwin Building, University College London, Gower St, London, WC1E 6BT, UK
Nat Methods 5:214-5; author reply 215. 2008
- Dietary restriction in DrosophilaLinda Partridge
UCL Centre for Research on Ageing, Department of Biology, University College London, London WC1E 6BT, UK
Mech Ageing Dev 126:938-50. 2005..This rapid mortality rate recovery provides a powerful framework within which to further investigate the mechanisms by which DR extends lifespan...
- Quantification of food intake in DrosophilaRichard Wong
Institute of Healthy Ageing, and GEE, University College London, London, UK
PLoS ONE 4:e6063. 2009..This approach provides a practical and reliable method for quantification of food intake in Drosophila under normal, undisturbed culture conditions...
- Amino-acid imbalance explains extension of lifespan by dietary restriction in DrosophilaRichard C Grandison
Institute of Healthy Ageing, Department of Genetics Evolution and Environment, University College London, UK
Nature 462:1061-4. 2009..In other organisms, including mammals, it may be possible to obtain the benefits to lifespan of dietary restriction without incurring a reduction in fecundity, through a suitable balance of nutrients in the diet...
- Dietary restriction in Drosophila: delayed aging or experimental artefact?Matthew D W Piper
University College London Centre for Research on Ageing, Department of Biology, University College London, London, United Kingdom
PLoS Genet 3:e57. 2007..We also indicate some experiments that could be performed to determine if DR in Drosophila operates through similar mechanisms to the process in rodents...
- Coordinated multitissue transcriptional and plasma metabonomic profiles following acute caloric restriction in miceColin Selman
Centre for Diabetes and Endocrinology, Department of Medicine, University College London, Rayne Institute, London, United Kingdom
Physiol Genomics 27:187-200. 2006..Mice undergoing acute CR rapidly adopt many transcriptional and metabolic changes of long-term CR, suggesting that the beneficial effects of CR may require only a short-term reduction in caloric intake...
- Using doubly-labeled water to measure energy expenditure in an important small ectotherm Drosophila melanogasterMatthew D W Piper
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, Gower Street, London, WC1E 6BT, UK
J Genet Genomics 41:505-12. 2014..This approach may be of considerable value in future ageing research attempting to understand the genetic and genomic basis of ageing...
- Target of rapamycin signalling mediates the lifespan-extending effects of dietary restriction by essential amino acid alterationSahar Emran
Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London WC1E 6BT, United Kingdom
Aging (Albany NY) 6:390-8. 2014..These data indicate that lowered dietary amino acids promote longevity via TOR, not by enhanced resistance to molecular damage, but through modified physiological conditions that favour fat accumulation. ..
- Detrimental effects of RNAi: a cautionary note on its use in Drosophila ageing studiesNazif Alic
Institute of Healthy Ageing, and GEE, University College London, London, United Kingdom
PLoS ONE 7:e45367. 2012..However, we also show that RNAi restricted to certain tissues may not be detrimental to lifespan...
- Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligandsSusan J Broughton
Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
Proc Natl Acad Sci U S A 102:3105-10. 2005..However, the ablated flies show an extension of median and maximal lifespan and increased resistance to oxidative stress and starvation...
- The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfurViktor M Boer
Kluyver Laboratory of Biotechnology, Technical University of Delft, Julianalaan 67, 2628BC Delft, The Netherlands
J Biol Chem 278:3265-74. 2003..The transcription of these genes can be used as indicators for the characterization of nutrient-limited growth conditions and provide information for metabolic engineering strategies...
- Identification and characterization of phenylpyruvate decarboxylase genes in Saccharomyces cerevisiaeZeynep Vuralhan
Kluyver Laboratory of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands
Appl Environ Microbiol 69:4534-41. 2003..Modifications to this gene could therefore be important for metabolic engineering of the Ehrlich pathway...
- Identification of a novel one-carbon metabolism regulon in Saccharomyces cerevisiaeCristy L Gelling
Ramaciotti Centre for Gene Function Analysis and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
J Biol Chem 279:7072-81. 2004..The one-carbon response is distinct from the Bas1p purine biosynthesis regulon and thus represents the first example of transcriptional regulation in response to activated one-carbon status...
- Directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, yielding a C2-independent, glucose-tolerant, and pyruvate-hyperproducing yeastAntonius J A van Maris
Department of Biotechnology, Delft University of Technology, NL 2628 BC Delft BIRD Engineering B V, NL 3044 CK Rotterdam, The Netherlands
Appl Environ Microbiol 70:159-66. 2004..54 g of pyruvate g of glucose(-1)...
- Prolonged maltose-limited cultivation of Saccharomyces cerevisiae selects for cells with improved maltose affinity and hypersensitivityMickel L A Jansen
Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands
Appl Environ Microbiol 70:1956-63. 2004..The observed trade-off between substrate affinity and substrate tolerance may be relevant for metabolic engineering and strain selection for utilization of substrates that are taken up by proton symport...
- Transcriptional responses of Saccharomyces cerevisiae to preferred and nonpreferred nitrogen sources in glucose-limited chemostat culturesViktor M Boer
Department of Biotechnology, Delft University of Technology, Julianalaan, Delft, The Netherlands
FEMS Yeast Res 7:604-20. 2007..By this criterion, leucine, proline and methionine were 'poor' nitrogen sources, and phenylalanine showed an 'intermediate' NCR response...
- Reproducibility of oligonucleotide microarray transcriptome analyses. An interlaboratory comparison using chemostat cultures of Saccharomyces cerevisiaeMatthew D W Piper
Kluyver Laboratory of Biotechnology, Technical University of Delft, Julianalaan 26, Delft 2628BC, The Netherlands
J Biol Chem 277:37001-8. 2002....