Genomes and Genes
Affiliation: University of Cambridge
- Emi1 is needed to couple DNA replication with mitosis but does not regulate activation of the mitotic APC/CBarbara Di Fiore
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, England, UK
J Cell Biol 177:425-37. 2007..Thus, Emi1 plays a crucial role in the cell cycle to couple DNA replication with mitosis, and our results also question the current view that the APC/C has to be inactivated to allow DNA replication...
- Mitosis: a matter of getting rid of the right protein at the right timeJonathon Pines
Wellcome Cancer Research UK Gurdon Institute and Dept of Zoology, Tennis Court Road, Cambridge, UK CB2 1QN
Trends Cell Biol 16:55-63. 2006....
- APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachmentJörg Mansfeld
The Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
Nat Cell Biol 13:1234-43. 2011..We conclude that APC15 mediates the constant turnover of CDC20 and MCCs on the APC/C to allow the SAC to respond to the attachment state of kinetochores...
- How cyclin A destruction escapes the spindle assembly checkpointBarbara Di Fiore
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, England, UK
J Cell Biol 190:501-9. 2010..Subsequently, the Cks protein is necessary and sufficient to promote cyclin A degradation in the presence of an active checkpoint by binding cyclin A-Cdc20 to the APC/C...
- How APC/C-Cdc20 changes its substrate specificity in mitosisDaisuke Izawa
Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
Nat Cell Biol 13:223-33. 2011..Moreover, APC10 is crucial for the destruction of cyclin B1 and securin, but not cyclin A. We conclude that the SAC causes Cdc20 to bind to different sites on the APC/C and this alters APC/C substrate specificity...
- Activation of cyclin B1-Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosisOlivier Gavet
The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK
J Cell Biol 189:247-59. 2010..Thus, the intrinsic link between cyclin B1-Cdk1 activation and its rapid nuclear import inherently coordinates the reorganization of the nucleus and the cytoplasm at mitotic entry...
- Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cellsCatherine Lindon
Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Rd, Cambridge CB2 1QR, England, UK
J Cell Biol 164:233-41. 2004..Our experiments reveal a role for APC/C-mediated proteolysis in exit from mitosis in human cells...
- UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exitMathew J Garnett
University of Cambridge, Department of Oncology and The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 OXZ, UK
Nat Cell Biol 11:1363-9. 2009..Thus, UBE2S functions with the APC/C in a two-step mechanism to control substrate ubiquitylation that is essential for mitotic exit after prolonged SAC activation, providing a new model for APC/C function in human cells...
- Chfr acts with the p38 stress kinases to block entry to mitosis in mammalian cellsTakahiro Matsusaka
Wellcome Cancer Research UK Gurdon Institute, and Department of Zoology, Tennis Court Rd, University of Cambridge, Cambridge CB2 1QR, England, UK
J Cell Biol 166:507-16. 2004....
- APC/C Cdh1 targets aurora kinase to control reorganization of the mitotic spindle at anaphaseSuzanne Floyd
Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
Curr Biol 18:1649-58. 2008..Here, we have investigated the role of APC/C(Cdh1) at this time in the cell cycle by using siRNA-mediated depletion of Cdh1 in human cells...
- Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosisOlivier Gavet
The Gurdon Institute and Department of Zoology, Tennis Court Road, CB2 1QN Cambridge, UK
Dev Cell 18:533-43. 2010....
- Active cyclin B1-Cdk1 first appears on centrosomes in prophaseMark Jackman
Wellcome Trust Cancer Research UK Institute and Department of Zoology, Tennis Court Road, Cambridge, CB2 1QR, UK
Nat Cell Biol 5:143-8. 2003..We conclude that cyclin B1-Cdk1 is first activated in the cytoplasm and that centrosomes may function as sites of integration for the proteins that trigger mitosis...
- The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destructionJakob Nilsson
Wellcome CR UK Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
Nat Cell Biol 10:1411-20. 2008..We further show that during the SAC, the APC/C ubiquitylates Cdc20 to target it for degradation. Thus, ubiquitylation of human Cdc20 is not required to release it from the checkpoint complex, but to degrade it to maintain mitotic arrest...
- Quantitative proteomics reveals the basis for the biochemical specificity of the cell-cycle machineryFelicia Walton Pagliuca
The Gurdon Institute, University of Cambridge, Cambridge, UK
Mol Cell 43:406-17. 2011..Furthermore, we identify key substrates (Wee1 and c15orf42/Sld3) that reveal how cyclin A is able to promote both DNA replication and mitosis...
- Defining the role of Emi1 in the DNA replication-segregation cycleBarbara Di Fiore
Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
Chromosoma 117:333-8. 2008..In this review we discuss new evidence that indicates that Emi1 is essential to restrict APC/C activity in interphase and, by doing so, ensure the proper coordination between DNA replication and mitosis...
- Proteolysis: anytime, any place, anywhere?Jonathon Pines
Wellcome Trust Cancer Research UK, Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
Nat Cell Biol 7:731-5. 2005..Controlling where a protein is degraded can enhance the specificity and timing of proteolysis, generate asymmetry and maintain sub-compartments even in the mitotic cell. Here, we discuss this aspect of the UPS...
- The centrosome opens the way to mitosisRenata Basto
Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge, CB2 1QR United Kingdom
Dev Cell 12:475-7. 2007..2007). Surprisingly, the role of the centrosome in this process is independent of its role as a microtubule nucleation organelle. Instead, the centrosome seems to act as a spatial regulator for the activation of the Aurora A kinase...
- Mad2 and the APC/C compete for the same site on Cdc20 to ensure proper chromosome segregationDaisuke Izawa
The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK
J Cell Biol 199:27-37. 2012..Thus, we provide insights into how Cdc20 binds the APC/C and uncover a second mechanism by which the SAC inhibits the APC/C...
- The Renaissance or the cuckoo clockJonathon Pines
Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
Philos Trans R Soc Lond B Biol Sci 366:3625-34. 2011....
- UbcH10 has a rate-limiting role in G1 phase but might not act in the spindle checkpoint or as part of an autonomous oscillatorAdam Walker
Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Cambridge CB2 1QN, UK
J Cell Sci 121:2319-26. 2008..Instead, we find that the rate-limiting role of UbcH10 is only at the end of G1 phase, just before DNA replication begins...
- Cubism and the cell cycle: the many faces of the APC/CJonathon Pines
The Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
Nat Rev Mol Cell Biol 12:427-38. 2011..Remarkably, one protein complex is responsible for this surgical precision, the APC/C (anaphase-promoting complex, also known as the cyclosome). The APC/C is tightly regulated by its co-activators and by the spindle assembly checkpoint...
- The anaphase promoting complex/cyclosome is recruited to centromeres by the spindle assembly checkpointClaire Acquaviva
Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
Nat Cell Biol 6:892-8. 2004..Indeed, we provide evidence that the spindle checkpoint machinery is required to recruit the APC/C to kinetochores. Our data indicate that the APC/C could be regulated directly by the spindle checkpoint...
- The anaphase-promoting complex/cyclosome: APC/CClaire Acquaviva
Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge, CB2 1QN, UK
J Cell Sci 119:2401-4. 2006
- The APC/C: a smörgåsbord for proteolysisJonathon Pines
Gurdon Institute, Tennis Court Road, Cambridge, UK
Mol Cell 34:135-6. 2009....
- Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1Lorena Clay-Farrace
MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK
EMBO J 22:704-12. 2003..Thus, HuCdc6 can trigger a checkpoint response, which could ensure that all DNA is replicated before mitotic entry. We also present evidence that the ability of HuCdc6 to block mitosis may be regulated by its phosphorylation...
- Cyclin A- and cyclin E-Cdk complexes shuttle between the nucleus and the cytoplasmMark Jackman
Wellcome Cancer Research U K London Institute and Department of Zoology, University of Cambridge, Cambridge, United Kingdom CB2 1QR
Mol Biol Cell 13:1030-45. 2002..This has implications for the role of the hydrophobic patch as a substrate selection motif...
- Cell cycle trials in Salamanca: workshop on G2/M progression and associated checkpointsJonathon Pines
Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
EMBO Rep 3:17-21. 2002
- The spindle assembly checkpoint works like a rheostat rather than a toggle switchPhilippe Collin
The Gurdon Institute, and Department of Zoology, University of Cambridge, Cambridge CB2 1QN, UK
Nat Cell Biol 15:1378-85. 2013..Furthermore, we show that different drugs activate the SAC to different extents, which may be relevant to their efficacy in chemotherapy...
- Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin ARob Wolthuis
Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
Mol Cell 30:290-302. 2008..Thus, we identify a crucial role for the Cks proteins in mitosis and one mechanism by which the APC/C can target substrates independently of the spindle checkpoint...
- Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteinsIvan Ahel
Genetic Recombination and, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
Nature 451:81-5. 2008....
- Meeting report: International Symposium on 'Ran and the Cell Cycle', October 2-5, 2005, Awaji Yumebutai, JapanMary Dasso
Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD 20892 5431, USA
Traffic 7:474-8. 2006
- Mitotic regulation of the human anaphase-promoting complex by phosphorylationClaudine Kraft
Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, 1030 Vienna, Austria
EMBO J 22:6598-609. 2003..Plk1 depletion neither prevents APC phosphorylation nor cyclin A destruction in vivo. These observations imply that APC activation is initiated by Cdk1 already in the nuclei of late prophase cells...
- Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclinThomas B Nguyen
Department of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
J Biol Chem 277:41960-9. 2002..The mouse cDNA has allowed us to further localize cyclin B3 mRNA to leptotene and zygotene spermatocytes. The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I...
- Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC/C switches from activation by Cdc20 to Cdh1Anja Hagting
Wellcome Cancer Research UK Institute, Cambridge CB2 1QR, United Kingdom
J Cell Biol 157:1125-37. 2002..Thus, defects in securin destruction alter chromosome segregation and may be relevant to the development of aneuploidy in cancer...
- Cell division, growth and death. Cell growth: live and let dieSally Kornbluth
Curr Opin Cell Biol 15:645-7. 2003