Jonathon Pines

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc Emi1 is needed to couple DNA replication with mitosis but does not regulate activation of the mitotic APC/C
    Barbara Di Fiore
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, England, UK
    J Cell Biol 177:425-37. 2007
  2. ncbi request reprint Mitosis: a matter of getting rid of the right protein at the right time
    Jonathon Pines
    Wellcome Cancer Research UK Gurdon Institute and Dept of Zoology, Tennis Court Road, Cambridge, UK CB2 1QN
    Trends Cell Biol 16:55-63. 2006
  3. pmc APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment
    Jörg Mansfeld
    The Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Cell Biol 13:1234-43. 2011
  4. pmc How cyclin A destruction escapes the spindle assembly checkpoint
    Barbara Di Fiore
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, England, UK
    J Cell Biol 190:501-9. 2010
  5. pmc How APC/C-Cdc20 changes its substrate specificity in mitosis
    Daisuke Izawa
    Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Cell Biol 13:223-33. 2011
  6. pmc Activation of cyclin B1-Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis
    Olivier Gavet
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK
    J Cell Biol 189:247-59. 2010
  7. pmc Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cells
    Catherine Lindon
    Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Rd, Cambridge CB2 1QR, England, UK
    J Cell Biol 164:233-41. 2004
  8. pmc UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit
    Mathew J Garnett
    University of Cambridge, Department of Oncology and The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 OXZ, UK
    Nat Cell Biol 11:1363-9. 2009
  9. pmc Chfr acts with the p38 stress kinases to block entry to mitosis in mammalian cells
    Takahiro Matsusaka
    Wellcome Cancer Research UK Gurdon Institute, and Department of Zoology, Tennis Court Rd, University of Cambridge, Cambridge CB2 1QR, England, UK
    J Cell Biol 166:507-16. 2004
  10. doi request reprint APC/C Cdh1 targets aurora kinase to control reorganization of the mitotic spindle at anaphase
    Suzanne Floyd
    Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Curr Biol 18:1649-58. 2008

Collaborators

Detail Information

Publications36

  1. pmc Emi1 is needed to couple DNA replication with mitosis but does not regulate activation of the mitotic APC/C
    Barbara Di Fiore
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, England, UK
    J Cell Biol 177:425-37. 2007
    ..Thus, Emi1 plays a crucial role in the cell cycle to couple DNA replication with mitosis, and our results also question the current view that the APC/C has to be inactivated to allow DNA replication...
  2. ncbi request reprint Mitosis: a matter of getting rid of the right protein at the right time
    Jonathon Pines
    Wellcome Cancer Research UK Gurdon Institute and Dept of Zoology, Tennis Court Road, Cambridge, UK CB2 1QN
    Trends Cell Biol 16:55-63. 2006
    ....
  3. pmc APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment
    Jörg Mansfeld
    The Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Cell Biol 13:1234-43. 2011
    ..We conclude that APC15 mediates the constant turnover of CDC20 and MCCs on the APC/C to allow the SAC to respond to the attachment state of kinetochores...
  4. pmc How cyclin A destruction escapes the spindle assembly checkpoint
    Barbara Di Fiore
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, England, UK
    J Cell Biol 190:501-9. 2010
    ..Subsequently, the Cks protein is necessary and sufficient to promote cyclin A degradation in the presence of an active checkpoint by binding cyclin A-Cdc20 to the APC/C...
  5. pmc How APC/C-Cdc20 changes its substrate specificity in mitosis
    Daisuke Izawa
    Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Cell Biol 13:223-33. 2011
    ..Moreover, APC10 is crucial for the destruction of cyclin B1 and securin, but not cyclin A. We conclude that the SAC causes Cdc20 to bind to different sites on the APC/C and this alters APC/C substrate specificity...
  6. pmc Activation of cyclin B1-Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis
    Olivier Gavet
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK
    J Cell Biol 189:247-59. 2010
    ..Thus, the intrinsic link between cyclin B1-Cdk1 activation and its rapid nuclear import inherently coordinates the reorganization of the nucleus and the cytoplasm at mitotic entry...
  7. pmc Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cells
    Catherine Lindon
    Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Rd, Cambridge CB2 1QR, England, UK
    J Cell Biol 164:233-41. 2004
    ..Our experiments reveal a role for APC/C-mediated proteolysis in exit from mitosis in human cells...
  8. pmc UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit
    Mathew J Garnett
    University of Cambridge, Department of Oncology and The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 OXZ, UK
    Nat Cell Biol 11:1363-9. 2009
    ..Thus, UBE2S functions with the APC/C in a two-step mechanism to control substrate ubiquitylation that is essential for mitotic exit after prolonged SAC activation, providing a new model for APC/C function in human cells...
  9. pmc Chfr acts with the p38 stress kinases to block entry to mitosis in mammalian cells
    Takahiro Matsusaka
    Wellcome Cancer Research UK Gurdon Institute, and Department of Zoology, Tennis Court Rd, University of Cambridge, Cambridge CB2 1QR, England, UK
    J Cell Biol 166:507-16. 2004
    ....
  10. doi request reprint APC/C Cdh1 targets aurora kinase to control reorganization of the mitotic spindle at anaphase
    Suzanne Floyd
    Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Curr Biol 18:1649-58. 2008
    ..Here, we have investigated the role of APC/C(Cdh1) at this time in the cell cycle by using siRNA-mediated depletion of Cdh1 in human cells...
  11. pmc Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis
    Olivier Gavet
    The Gurdon Institute and Department of Zoology, Tennis Court Road, CB2 1QN Cambridge, UK
    Dev Cell 18:533-43. 2010
    ....
  12. ncbi request reprint Active cyclin B1-Cdk1 first appears on centrosomes in prophase
    Mark Jackman
    Wellcome Trust Cancer Research UK Institute and Department of Zoology, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 5:143-8. 2003
    ..We conclude that cyclin B1-Cdk1 is first activated in the cytoplasm and that centrosomes may function as sites of integration for the proteins that trigger mitosis...
  13. pmc The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction
    Jakob Nilsson
    Wellcome CR UK Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Cell Biol 10:1411-20. 2008
    ..We further show that during the SAC, the APC/C ubiquitylates Cdc20 to target it for degradation. Thus, ubiquitylation of human Cdc20 is not required to release it from the checkpoint complex, but to degrade it to maintain mitotic arrest...
  14. pmc Quantitative proteomics reveals the basis for the biochemical specificity of the cell-cycle machinery
    Felicia Walton Pagliuca
    The Gurdon Institute, University of Cambridge, Cambridge, UK
    Mol Cell 43:406-17. 2011
    ..Furthermore, we identify key substrates (Wee1 and c15orf42/Sld3) that reveal how cyclin A is able to promote both DNA replication and mitosis...
  15. doi request reprint Defining the role of Emi1 in the DNA replication-segregation cycle
    Barbara Di Fiore
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
    Chromosoma 117:333-8. 2008
    ..In this review we discuss new evidence that indicates that Emi1 is essential to restrict APC/C activity in interphase and, by doing so, ensure the proper coordination between DNA replication and mitosis...
  16. ncbi request reprint Proteolysis: anytime, any place, anywhere?
    Jonathon Pines
    Wellcome Trust Cancer Research UK, Gurdon Institute and Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Cell Biol 7:731-5. 2005
    ..Controlling where a protein is degraded can enhance the specificity and timing of proteolysis, generate asymmetry and maintain sub-compartments even in the mitotic cell. Here, we discuss this aspect of the UPS...
  17. ncbi request reprint The centrosome opens the way to mitosis
    Renata Basto
    Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge, CB2 1QR United Kingdom
    Dev Cell 12:475-7. 2007
    ..2007). Surprisingly, the role of the centrosome in this process is independent of its role as a microtubule nucleation organelle. Instead, the centrosome seems to act as a spatial regulator for the activation of the Aurora A kinase...
  18. pmc Mad2 and the APC/C compete for the same site on Cdc20 to ensure proper chromosome segregation
    Daisuke Izawa
    The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK
    J Cell Biol 199:27-37. 2012
    ..Thus, we provide insights into how Cdc20 binds the APC/C and uncover a second mechanism by which the SAC inhibits the APC/C...
  19. pmc The Renaissance or the cuckoo clock
    Jonathon Pines
    Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
    Philos Trans R Soc Lond B Biol Sci 366:3625-34. 2011
    ....
  20. doi request reprint UbcH10 has a rate-limiting role in G1 phase but might not act in the spindle checkpoint or as part of an autonomous oscillator
    Adam Walker
    Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Cambridge CB2 1QN, UK
    J Cell Sci 121:2319-26. 2008
    ..Instead, we find that the rate-limiting role of UbcH10 is only at the end of G1 phase, just before DNA replication begins...
  21. doi request reprint Cubism and the cell cycle: the many faces of the APC/C
    Jonathon Pines
    The Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Rev Mol Cell Biol 12:427-38. 2011
    ..Remarkably, one protein complex is responsible for this surgical precision, the APC/C (anaphase-promoting complex, also known as the cyclosome). The APC/C is tightly regulated by its co-activators and by the spindle assembly checkpoint...
  22. ncbi request reprint The anaphase promoting complex/cyclosome is recruited to centromeres by the spindle assembly checkpoint
    Claire Acquaviva
    Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 6:892-8. 2004
    ..Indeed, we provide evidence that the spindle checkpoint machinery is required to recruit the APC/C to kinetochores. Our data indicate that the APC/C could be regulated directly by the spindle checkpoint...
  23. ncbi request reprint The anaphase-promoting complex/cyclosome: APC/C
    Claire Acquaviva
    Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge, CB2 1QN, UK
    J Cell Sci 119:2401-4. 2006
  24. doi request reprint The APC/C: a smörgåsbord for proteolysis
    Jonathon Pines
    Gurdon Institute, Tennis Court Road, Cambridge, UK
    Mol Cell 34:135-6. 2009
    ....
  25. pmc Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1
    Lorena Clay-Farrace
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK
    EMBO J 22:704-12. 2003
    ..Thus, HuCdc6 can trigger a checkpoint response, which could ensure that all DNA is replicated before mitotic entry. We also present evidence that the ability of HuCdc6 to block mitosis may be regulated by its phosphorylation...
  26. pmc Cyclin A- and cyclin E-Cdk complexes shuttle between the nucleus and the cytoplasm
    Mark Jackman
    Wellcome Cancer Research U K London Institute and Department of Zoology, University of Cambridge, Cambridge, United Kingdom CB2 1QR
    Mol Biol Cell 13:1030-45. 2002
    ..This has implications for the role of the hydrophobic patch as a substrate selection motif...
  27. pmc Cell cycle trials in Salamanca: workshop on G2/M progression and associated checkpoints
    Jonathon Pines
    Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO Rep 3:17-21. 2002
  28. pmc The spindle assembly checkpoint works like a rheostat rather than a toggle switch
    Philippe Collin
    The Gurdon Institute, and Department of Zoology, University of Cambridge, Cambridge CB2 1QN, UK
    Nat Cell Biol 15:1378-85. 2013
    ..Furthermore, we show that different drugs activate the SAC to different extents, which may be relevant to their efficacy in chemotherapy...
  29. doi request reprint Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A
    Rob Wolthuis
    Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Mol Cell 30:290-302. 2008
    ..Thus, we identify a crucial role for the Cks proteins in mitosis and one mechanism by which the APC/C can target substrates independently of the spindle checkpoint...
  30. doi request reprint Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins
    Ivan Ahel
    Genetic Recombination and, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Nature 451:81-5. 2008
    ....
  31. ncbi request reprint Meeting report: International Symposium on 'Ran and the Cell Cycle', October 2-5, 2005, Awaji Yumebutai, Japan
    Mary Dasso
    Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD 20892 5431, USA
    Traffic 7:474-8. 2006
  32. pmc Mitotic regulation of the human anaphase-promoting complex by phosphorylation
    Claudine Kraft
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, 1030 Vienna, Austria
    EMBO J 22:6598-609. 2003
    ..Plk1 depletion neither prevents APC phosphorylation nor cyclin A destruction in vivo. These observations imply that APC activation is initiated by Cdk1 already in the nuclei of late prophase cells...
  33. ncbi request reprint Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin
    Thomas B Nguyen
    Department of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 277:41960-9. 2002
    ..The mouse cDNA has allowed us to further localize cyclin B3 mRNA to leptotene and zygotene spermatocytes. The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I...
  34. pmc Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC/C switches from activation by Cdc20 to Cdh1
    Anja Hagting
    Wellcome Cancer Research UK Institute, Cambridge CB2 1QR, United Kingdom
    J Cell Biol 157:1125-37. 2002
    ..Thus, defects in securin destruction alter chromosome segregation and may be relevant to the development of aneuploidy in cancer...
  35. ncbi request reprint Cell division, growth and death. Cell growth: live and let die
    Sally Kornbluth
    Curr Opin Cell Biol 15:645-7. 2003