Alvin W Nienow
Affiliation: University of Birmingham
- Reactor engineering in large scale animal cell cultureAlvin W Nienow
Centre for Bioprocess Engineering, The University of Birmingham, Birmingham, B15 2TT, UK
Cytotechnology 50:9-33. 2006..Microcarrier culture is also briefly addressed. Finally, some recommendations for bioreactor configuration and operating strategy are given...
- Break-up, coalescence and catastrophic phase inversion in turbulent contactorsA W Nienow
Department of Chemical Engineering, School of Engineering, The University of Birmingham, Birmingham B15 2TT, UK
Adv Colloid Interface Sci 108:95-103. 2004..g. surfactants, particles that bridge interfaces, wettable surfaces, bulk flow patterns, encourages CPI to occur at lower concentrations of dispersed phase. Satisfactory models for CPI are not available...
- Studies supporting the use of mechanical mixing in large scale beer fermentationsAlvin W Nienow
Department of Chemical Engineering, Centre for Bioprocess Engineering, University of Birmingham, Birmingham, B15 2TT, UK
Biotechnol Lett 32:623-33. 2010..It is suggested that this reluctance of the brewing industry to use (adequate) mechanical agitation is another example where the myth of shear damage has had a detrimental effect on the optimal operation of commercial bioprocessing...
- Scale-down/scale-up studies leading to improved commercial beer fermentationAlvin W Nienow
Centre for Bioprocess Engineering, University of Birmingham, UK
Biotechnol J 6:911-25. 2011..Suggestions for further studies are made: one concerning the impact of homogenization on the removal of vicinal diketones and the other on the location of bubble formation at the commercial scale...
- Culture of human mesenchymal stem cells on microcarriers in a 5 l stirred-tank bioreactorQasim A Rafiq
Department of Chemical Engineering, Centre for Biological Engineering, Loughborough University, Leicestershire, LE11 3TU, UK
Biotechnol Lett 35:1233-45. 2013..Neither the upstream nor downstream bioprocessing unit operations had a discernible effect on cell quality with the harvested cells retaining their immunophenotypic markers, key morphological features and differentiation capacity...
- A quantitative approach for understanding small-scale human mesenchymal stem cell culture - implications for large-scale bioprocess developmentQasim A Rafiq
Centre for Biological Engineering, Department of Chemical Engineering, Loughborough University, Leicestershire LE11 3TU England, UK
Biotechnol J 8:459-71. 2013....
- Expansion of human mesenchymal stem cells on microcarriersChristopher J Hewitt
Department of Chemical Engineering, Centre for Biological Engineering, Loughborough University, Leicestershire, UK
Biotechnol Lett 33:2325-35. 2011..These findings were interpreted using Kolmogorov's theory of isotropic turbulence...
- The application of multi-parameter flow cytometry to the study of recombinant Escherichia coli batch fermentation processesGareth Lewis
School of Engineering Chemical Engineering, The University of Birmingham, B15 2TT, Edgbaston, UK
J Ind Microbiol Biotechnol 31:311-22. 2004..Further, we discuss the possibility of optimising inclusion body formation by manipulating the fermentation conditions based on these rapid "real-time" measurements...
- Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flaskAndrew J Want
Centre for Biological Engineering, Department of Chemical Engineering, Loughborough University, Leicestershire, UK
Regen Med 7:71-84. 2012..This represents the first step in defining the full extent of the challenges facing bioprocess engineers in the exploitation of large-scale human pluripotent stem cell manufacture...
- The use of phase inversion temperature (PIT) microemulsion technology to enhance oil utilisation during Streptomyces rimosus fed-batch fermentations to produce oxytetracyclinePanos A Papapanagiotou
Biochemical Engineering, Department of Chemical Engineering, The University of Birmingham, B15 2TT, Edgbaston, Birmingham, UK
Biotechnol Lett 27:1579-85. 2005..Importantly, microbial utilisation of the surfactant was confirmed for the first time...
- Further studies related to the scale-up of high cell density Escherichia coli fed-batch fermentations: the additional effect of a changing microenvironment when using aqueous ammonia to control pHHelen Onyeaka
Biochemical Engineering, Centre for Formulation Engineering, School of Engineering Chemical Engineering, The University of Birmingham, Edgbaston B15 2TT, UK
Biotechnol Bioeng 84:474-84. 2003....