Affiliation: University of Southampton
- Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolutionCameron Neylon
School of Chemistry, University of Southampton, Highfield SO17 1BJ, UK
Nucleic Acids Res 32:1448-59. 2004..References are provided to original methodology papers and other recent examples from the primary literature that provide details of experimental methods...
- An analysis of the feasibility of short read sequencingNava Whiteford
School of Chemistry, University of Southampton, Southampton SO17 1BJ, UK
Nucleic Acids Res 33:e171. 2005....
- Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complexCameron Neylon
School of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom
Microbiol Mol Biol Rev 69:501-26. 2005..The evidence suggests, therefore, that the termination system is more subtle and complex than may have been assumed. We describe some further experiments and insights that may assist in unraveling the details of this fascinating process...
- Diffractive micro bar codes for encoding of biomolecules in multiplexed assaysGraham R Broder
School of Chemistry, School of Electronics and Computer Science, Optoelectronics Research Centre, and School of Physics and Astronomy, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
Anal Chem 80:1902-9. 2008....
- Kinetics and thermodynamics of biotinylated oligonucleotide probe binding to particle-immobilized avidin and implications for multiplexing applicationsGraham R Broder
School of Chemistry, University of Southampton, Southampton SO17 1BJ, U K
Anal Chem 83:2005-11. 2011..These findings are critical for the design of multiplexed assays where probe molecules are immobilized to biosensors via the avidin-biotin interaction...
- Optimal probe length varies for targets with high sequence variation: implications for probe library design for resequencing highly variable genesNiall J Haslam
School of Chemistry, University of Southampton, Southhampton, United Kingdom
PLoS ONE 3:e2500. 2008..We have investigated the effect of probe length, use of reference sequences, and thermal filtering on the design of probe libraries for several highly variable target DNA sequences...
- Covalent attachment of proteins to solid supports and surfaces via Sortase-mediated ligationLilyan Chan
School of Chemistry, University of Southampton, Highfield, Southampton, United Kingdom
PLoS ONE 2:e1164. 2007..A critical requirement for these applications is the generation of a stable linkage between the solid support and the immobilized, but still functional, protein...
- Multistep synthesis on SU-8: combining microfabrication and solid-phase chemistry on a single materialGabriel Cavalli
School of Chemistry, Highfield Campus, University of Southampton, Southampton SO17 1BJ, UK
J Comb Chem 9:462-72. 2007....
- Introducing structural flexibility into porphyrin-DNA zipper arraysAshley Brewer
School of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ
Org Biomol Chem 9:777-82. 2011....
- A molecular mousetrap determines polarity of termination of DNA replication in E. coliMark D Mulcair
Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
Cell 125:1309-19. 2006..Lock formation specifically requires the cytosine residue, C(6). The crystal structure of the locked complex showed that C(6) moves 14 A from its normal position to bind in a cytosine-specific pocket on the surface of Tus...