A W Munro

Summary

Affiliation: University of Leicester
Country: UK

Publications

  1. ncbi request reprint Cytochromes P450: novel drug targets in the war against multidrug-resistant Mycobacterium tuberculosis
    A W Munro
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    Biochem Soc Trans 31:625-30. 2003
  2. ncbi request reprint Flavocytochrome P450 BM3: an update on structure and mechanism of a biotechnologically important enzyme
    A J Warman
    Department of Biochemistry, University of Leicester, The Henry Wellcome Building, Lancaster Road, Leicester LE1 9HN, UK
    Biochem Soc Trans 33:747-53. 2005
  3. ncbi request reprint P450 BM3: the very model of a modern flavocytochrome
    Andrew W Munro
    Department Biochemistry, University of Leicester, The Adrian Building, University Road, Leicester LE1 7RH, UK
    Trends Biochem Sci 27:250-7. 2002
  4. ncbi request reprint Biodiversity of cytochrome P450 redox systems
    K J McLean
    Department of Biochemistry, University of Leicester, The Henry Wellcome Building, Lancaster Road, Leicester LE1 9HN, UK
    Biochem Soc Trans 33:796-801. 2005
  5. ncbi request reprint alpha Arg-237 in Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein affords approximately 200-millivolt stabilization of the FAD anionic semiquinone and a kinetic block on full reduction to the dihydroquinone
    F Talfournier
    Departments of Biochemistry and Chemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 276:20190-6. 2001
  6. pmc Effects of environment on flavin reactivity in morphinone reductase: analysis of enzymes displaying differential charge near the N-1 atom and C-2 carbonyl region of the active-site flavin
    D H Craig
    Department of Biochemistry and Centre for Chemical Biology, University of Leicester, University Road, Leicester LE1 7RH, UK
    Biochem J 359:315-23. 2001
  7. ncbi request reprint Electron transfer in human cytochrome P450 reductase
    A Gutierrez
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    Biochem Soc Trans 31:497-501. 2003
  8. ncbi request reprint Biophysical characterization of the sterol demethylase P450 from Mycobacterium tuberculosis, its cognate ferredoxin, and their interactions
    Kirsty J McLean
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Sciences, University of Manchester, Jackson s Mill, P O Box 88, Sackville Street, Manchester M60 1QD, UK
    Biochemistry 45:8427-43. 2006
  9. ncbi request reprint Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactis
    Jonathan P Combe
    Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, University of Manchester, Jackson s Mill, Sackville Street, Manchester M60 1QD, United Kingdom
    J Biol Chem 281:17977-88. 2006
  10. ncbi request reprint The preponderance of P450s in the Mycobacterium tuberculosis genome
    Kirsty J McLean
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science and School of Life Sciences, University of Manchester, Jackson s Mill, Sackville Street, Manchester, UK, M60 1QD
    Trends Microbiol 14:220-8. 2006

Collaborators

Detail Information

Publications63

  1. ncbi request reprint Cytochromes P450: novel drug targets in the war against multidrug-resistant Mycobacterium tuberculosis
    A W Munro
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    Biochem Soc Trans 31:625-30. 2003
    ..The structure provides both a platform for investigation of structure/mechanism of cytochrome P450, and for design of inhibitor molecules as novel anti-tubercular agents...
  2. ncbi request reprint Flavocytochrome P450 BM3: an update on structure and mechanism of a biotechnologically important enzyme
    A J Warman
    Department of Biochemistry, University of Leicester, The Henry Wellcome Building, Lancaster Road, Leicester LE1 9HN, UK
    Biochem Soc Trans 33:747-53. 2005
    ..This paper focuses on recent developments in our understanding of structure and mechanism of this important enzyme and highlights important problems still to be resolved...
  3. ncbi request reprint P450 BM3: the very model of a modern flavocytochrome
    Andrew W Munro
    Department Biochemistry, University of Leicester, The Adrian Building, University Road, Leicester LE1 7RH, UK
    Trends Biochem Sci 27:250-7. 2002
    ..This article discusses the fundamental properties of P450 BM3 and how progress with this model P450 has affected our comprehension of P450 systems in general...
  4. ncbi request reprint Biodiversity of cytochrome P450 redox systems
    K J McLean
    Department of Biochemistry, University of Leicester, The Henry Wellcome Building, Lancaster Road, Leicester LE1 9HN, UK
    Biochem Soc Trans 33:796-801. 2005
    ..This paper examines some of the recent advances in our understanding of the biodiversity of the P450 redox apparatus, with a particular emphasis on the redox systems in the pathogen Mycobacterium tuberculosis...
  5. ncbi request reprint alpha Arg-237 in Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein affords approximately 200-millivolt stabilization of the FAD anionic semiquinone and a kinetic block on full reduction to the dihydroquinone
    F Talfournier
    Departments of Biochemistry and Chemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 276:20190-6. 2001
    ..Additionally, we show that electron transfer from trimethylamine dehydrogenase to alphaR237A ETF is severely compromised, because of impaired assembly of the electron transfer complex...
  6. pmc Effects of environment on flavin reactivity in morphinone reductase: analysis of enzymes displaying differential charge near the N-1 atom and C-2 carbonyl region of the active-site flavin
    D H Craig
    Department of Biochemistry and Centre for Chemical Biology, University of Leicester, University Road, Leicester LE1 7RH, UK
    Biochem J 359:315-23. 2001
    ..This finding may have general implications for flavoenzyme catalysis, since it has generally been assumed that positive charge in this region has a stabilizing effect on the reduced form of flavin...
  7. ncbi request reprint Electron transfer in human cytochrome P450 reductase
    A Gutierrez
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    Biochem Soc Trans 31:497-501. 2003
    ..These studies and the mechanism of electron transfer in CPR are reported herein...
  8. ncbi request reprint Biophysical characterization of the sterol demethylase P450 from Mycobacterium tuberculosis, its cognate ferredoxin, and their interactions
    Kirsty J McLean
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Sciences, University of Manchester, Jackson s Mill, P O Box 88, Sackville Street, Manchester M60 1QD, UK
    Biochemistry 45:8427-43. 2006
    ..Steady-state analysis of a M. tuberculosis class I redox system comprising flavoprotein reductase A (FprA), Fdx, and estriol-bound CYP51 indicates heme iron reduction as a rate-limiting step...
  9. ncbi request reprint Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactis
    Jonathan P Combe
    Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, University of Manchester, Jackson s Mill, Sackville Street, Manchester M60 1QD, United Kingdom
    J Biol Chem 281:17977-88. 2006
    ....
  10. ncbi request reprint The preponderance of P450s in the Mycobacterium tuberculosis genome
    Kirsty J McLean
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science and School of Life Sciences, University of Manchester, Jackson s Mill, Sackville Street, Manchester, UK, M60 1QD
    Trends Microbiol 14:220-8. 2006
    ..Elucidation of structure, function and metabolic roles will be essential in targeting the P450s as an 'Achilles heel' in this major human pathogen...
  11. ncbi request reprint The dimeric form of flavocytochrome P450 BM3 is catalytically functional as a fatty acid hydroxylase
    Rajasekhar Neeli
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, The University of Manchester, Jackson s Mill, UK
    FEBS Lett 579:5582-8. 2005
    ..Fatty acid oxidation is reconstituted by mixing inactive oxygenase (A264H) and FMN-depleted (G570D) mutants, demonstrating that inter-monomer (FMN(1)-to-heme(2)) electron transfer supports oxygenase activity in the BM3 dimer...
  12. doi request reprint Inter-flavin electron transfer in cytochrome P450 reductase - effects of solvent and pH identify hidden complexity in mechanism
    Sibylle Brenner
    Manchester Interdisciplinary Biocentre and Faculty of Life Sciences, University of Manchester, UK
    FEBS J 275:4540-57. 2008
    ..Our analysis of the pH and isotope dependence in human CPR reveals previously hidden complexity in the mechanism of electron transfer in this complex flavoprotein...
  13. doi request reprint Trp(359) regulates flavin thermodynamics and coenzyme selectivity in Mycobacterium tuberculosis FprA
    Rajasekhar Neeli
    Manchester Interdisciplinary Biocentre, The University of Manchester, Faculty of Life Sciences, 131 Princess Street, Manchester M1 7DN, UK
    Biochem J 411:563-70. 2008
    ..The results demonstrate that the conserved Trp(359) is critical in regulating FprA FAD binding, thermodynamic properties, catalytic efficiency and coenzyme selectivity...
  14. ncbi request reprint The human apoptosis-inducing protein AMID is an oxidoreductase with a modified flavin cofactor and DNA binding activity
    Ker R Marshall
    Department of Biochemistry, University of Leicester, Henry Wellcome Building, Lancaster Road, Leicester LE1 9NH, United Kingdom
    J Biol Chem 280:30735-40. 2005
    ..Apo-AMID retains DNA binding activity. Our studies establish a link between coenzyme and DNA binding that likely impacts on the physiological role of AMID in cellular apoptosis...
  15. ncbi request reprint Role of active site residues and solvent in proton transfer and the modulation of flavin reduction potential in bacterial morphinone reductase
    Hanan L Messiha
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH
    J Biol Chem 280:27103-10. 2005
    ....
  16. ncbi request reprint Switching pyridine nucleotide specificity in P450 BM3: mechanistic analysis of the W1046H and W1046A enzymes
    Rajasekhar Neeli
    Department of Biochemistry, University of Leicester, The Adrian Building, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 280:17634-44. 2005
    ....
  17. ncbi request reprint Reaction of morphinone reductase with 2-cyclohexen-1-one and 1-nitrocyclohexene: proton donation, ligand binding, and the role of residues Histidine 186 and Asparagine 189
    Hanan Latif Messiha
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    J Biol Chem 280:10695-709. 2005
    ..The work highlights key mechanistic differences in the reactions catalyzed by MR and OYE1 and emphasizes the need for caution in inferring mechanistic similarities in structurally related proteins...
  18. ncbi request reprint Cytochrome P450--redox partner fusion enzymes
    Andrew W Munro
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, 131 Princess Street, Manchester, M1 7ND, UK
    Biochim Biophys Acta 1770:345-59. 2007
    ..This review examines our current state of knowledge of the diversity of these fusion proteins and explores their structural composition and evolutionary origins...
  19. ncbi request reprint Crystal structure of the Mycobacterium tuberculosis P450 CYP121-fluconazole complex reveals new azole drug-P450 binding mode
    Harriet E Seward
    Department of Biochemistry, University of Leicester, Henry Wellcome Building, Lancaster Road, Leicester LE6 0HQ, United Kingdom
    J Biol Chem 281:39437-43. 2006
    ....
  20. ncbi request reprint Structural and spectroscopic characterization of P450 BM3 mutants with unprecedented P450 heme iron ligand sets. New heme ligation states influence conformational equilibria in P450 BM3
    Hazel M Girvan
    School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK
    J Biol Chem 282:564-72. 2007
    ....
  21. ncbi request reprint Cytochrome P450/redox partner fusion enzymes: biotechnological and toxicological prospects
    Kirsty J McLean
    University of Manchester, Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, 131 Princess Street, Manchester M1 7DN, UK
    Expert Opin Drug Metab Toxicol 3:847-63. 2007
    ..Protein engineering has been widely applied to produce variant CYP fusions with desirable activities. The review focuses on the nature and diversity of CYP/redox partner fusion enzymes and their biocatalytic potential...
  22. ncbi request reprint Analysis of the interactions of cytochrome b5 with flavocytochrome P450 BM3 and its domains
    Michael A Noble
    Anadys Pharmaceuticals, Inc, San Diego, California, USA
    Drug Metab Rev 39:599-617. 2007
    ....
  23. ncbi request reprint DNA binding suppresses human AIF-M2 activity and provides a connection between redox chemistry, reactive oxygen species, and apoptosis
    Min Gong
    Manchester Interdisciplinary Biocentre and Faculty of Life Sciences, University of Manchester, M1 7DN Manchester, United Kingdom
    J Biol Chem 282:30331-40. 2007
    ..Through its DNA binding activity, we suggest that AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro)viral) cytosolic DNA, thus contributing to the onset of apoptosis...
  24. ncbi request reprint Conformational dynamics of the cytochrome P450 BM3/N-palmitoylglycine complex: the proposed "proximal-distal" transition probed by temperature-jump spectroscopy
    Sibylle Brenner
    Manchester Interdisciplinary Biocentre and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom
    J Phys Chem B 111:7879-86. 2007
    ..5 A away from the heme iron ("distal") and the catalytically relevant "proximal" binding site, and are in accordance with results from X-ray crystallography, NMR studies, and molecular dynamics simulations...
  25. ncbi request reprint Rapid P450 heme iron reduction by laser photoexcitation of Mycobacterium tuberculosis CYP121 and CYP51B1. Analysis of CO complexation reactions and reversibility of the P450/P420 equilibrium
    Adrian J Dunford
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN
    J Biol Chem 282:24816-24. 2007
    ..Our data provide strong evidence for P420 formation linked to reversible heme thiolate protonation, and demonstrate key differences in heme chemistry and CO binding for CYP121 and CYP51B1...
  26. ncbi request reprint Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily
    Andrew W Munro
    Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
    Nat Prod Rep 24:585-609. 2007
  27. ncbi request reprint Structure, function and drug targeting in Mycobacterium tuberculosis cytochrome P450 systems
    Kirsty J McLean
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Arch Biochem Biophys 464:228-40. 2007
    ....
  28. ncbi request reprint Laser photoexcitation of NAD(P)H induces reduction of P450 BM3 heme domain on the microsecond time scale
    Hazel M Girvan
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, U K
    J Am Chem Soc 129:6647-53. 2007
    ..This study demonstrates the applicability of NAD(P)H photoexcitation as a method for rapid electron delivery to P450 enzymes and provides a new route to probing the P450 catalytic cycle and its transient intermediates...
  29. ncbi request reprint Structural biology and biochemistry of cytochrome P450 systems in Mycobacterium tuberculosis
    Kirsty J McLean
    Manchester Interdisciplinary Biocentre, The University of Manchester, Faculty of Life Sciences, Manchester, M1, UK
    Drug Metab Rev 40:427-46. 2008
    ..Structural, biochemical and mechanistic studies on these systems have demonstrated their potential as antitubercular targets, as well as revealing novel aspects of P450 form and function...
  30. ncbi request reprint Conformational and thermodynamic control of electron transfer in neuronal nitric oxide synthase
    Adrian J Dunford
    Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Biochemistry 46:5018-29. 2007
    ..These results are discussed in light of the recent crystal structure of the NADPH-locked reductase domain...
  31. ncbi request reprint Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase. Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181, His184
    Huma Khan
    Department of Biochemistry, University of Leicester, UK
    FEBS J 272:4660-71. 2005
    ..Our data highlight mechanistic differences between Old Yellow Enzyme and PETN reductase and indicate that catalysis requires a metastable enzyme-steroid complex and not the most stable complex observed in crystallographic studies...
  32. ncbi request reprint Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis
    Christopher G Mowat
    Institute of Cell and Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland
    Acta Crystallogr D Biol Crystallogr 58:704-5. 2002
    ..3, c = 265.6 A. Native data have been collected to 1.6 A resolution and Hg-derivative data to 2.5 A resolution using a synchrotron-radiation source...
  33. ncbi request reprint Expression, purification and spectroscopic characterization of the cytochrome P450 CYP121 from Mycobacterium tuberculosis
    Kirsty J McLean
    Department of Biochemistry, The Adrian Building, University of Leicester, UK
    J Inorg Biochem 91:527-41. 2002
    ..CYP121 binds very tightly to a range of azole antifungal drugs (e.g. clotrimazole, miconazole), suggesting that it may represent a novel target for these antibiotics in the M. tuberculosis pathogen...
  34. ncbi request reprint Electron transfer in flavocytochrome P450 BM3: kinetics of flavin reduction and oxidation, the role of cysteine 999, and relationships with mammalian cytochrome P450 reductase
    Olivier Roitel
    Department of Biochemistry, University of Leicester, The Adrian Building, University Road, Leicester LE1 7RH, United Kingdom
    Biochemistry 42:10809-21. 2003
    ..The results are discussed in the light of kinetic and structural studies on mammalian cytochrome P450 reductase...
  35. ncbi request reprint Characterization of the cobaltochelatase CbiXL: evidence for a 4Fe-4S center housed within an MXCXXC motif
    Helen K Leech
    School of Biological Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    J Biol Chem 278:41900-7. 2003
    ..The potential physiological role of the iron-sulfur center is discussed...
  36. ncbi request reprint Atomic structure of Mycobacterium tuberculosis CYP121 to 1.06 A reveals novel features of cytochrome P450
    David Leys
    Department of Biochemistry, University of Leicester, The Adrian Building, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 278:5141-7. 2003
    ..Most interestingly, the electron density indicates weak binding of a dioxygen molecule to the P450. This structure provides a basis for rational design of putative antimycobacterial agents...
  37. ncbi request reprint Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding. Relaxation kinetic studies with coenzyme analogues
    Aldo Gutierrez
    Department of Biochemistry and Biological NMR Centre, University of Leicester, UK
    Eur J Biochem 270:2612-21. 2003
    ..e. enhanced at low coenzyme concentration) observed in stopped-flow studies. The data are discussed in the context of previous kinetic studies and of the crystallographic structure of rat CPR...
  38. ncbi request reprint Azole antifungals are potent inhibitors of cytochrome P450 mono-oxygenases and bacterial growth in mycobacteria and streptomycetes
    Kirsty J McLean
    Department of Biochemistry, University of Leicester, The Adrian Building, University Road, UK
    Microbiology 148:2937-49. 2002
    ..This suggests that azole drug therapy may provide a novel antibiotic strategy against strains of M. tuberculosis that have already developed resistance to isoniazid and other front-line drugs...
  39. ncbi request reprint Molecular dissection of human methionine synthase reductase: determination of the flavin redox potentials in full-length enzyme and isolated flavin-binding domains
    Kirsten R Wolthers
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Biochemistry 42:3911-20. 2003
    ..The redox properties of MSR are discussed in relation to other members of the diflavin oxidoreductase family and the mechanism of electron transfer...
  40. ncbi request reprint Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1
    Robert D Finn
    Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK
    Eur J Biochem 270:1164-75. 2003
    ..Despite overall structural resemblance of NR1 and CPR, our studies reveal thermodynamic similarities but major kinetic differences in the electron transfer reactions catalysed by the flavin-binding domains...
  41. pmc Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA
    Kirsty J McLean
    Department of Biochemistry, University of Leicester, The Adrian Building, University Road, UK
    Biochem J 372:317-27. 2003
    ..J. Biochem. 269, 3005-3013]...
  42. ncbi request reprint Expression, purification and characterisation of a Bacillus subtilis ferredoxin: a potential electron transfer donor to cytochrome P450 BioI
    Amanda J Green
    Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK
    J Inorg Biochem 93:92-9. 2003
    ..87+/-0.10 microM. Anaerobic experiments using CO-saturated buffer indicate that Fer is indeed capable of transferring electrons to this cytochrome P450 albeit at a fairly low rate...
  43. ncbi request reprint A single mutation in cytochrome P450 BM3 induces the conformational rearrangement seen upon substrate binding in the wild-type enzyme
    M Gordon Joyce
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, United Kingdom
    J Biol Chem 279:23287-93. 2004
    ....
  44. ncbi request reprint Flavocytochrome P450 BM3 mutant A264E undergoes substrate-dependent formation of a novel heme iron ligand set
    Hazel M Girvan
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, United Kingdom
    J Biol Chem 279:23274-86. 2004
    ..Glutamate coordination of the heme iron is confirmed by structural studies of the A264E mutant (Joyce, M. G., Girvan, H. M., Munro, A. W., and Leys, D. (2004) J. Biol. Chem. 279, 23287-23293)...
  45. ncbi request reprint Kinetic and structural basis of reactivity of pentaerythritol tetranitrate reductase with NADPH, 2-cyclohexenone, nitroesters, and nitroaromatic explosives
    Huma Khan
    Department of Biochemistry and Centre for Chemical Biology, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 277:21906-12. 2002
    ..Our studies provide a structural and mechanistic rationale for the ability of PETN reductase to react with the nitroaromatic explosive compounds TNT and picric acid and for the inhibition of enzyme activity with 2,4-DNP...
  46. ncbi request reprint Identification and characterization of a novel vitamin B12 (cobalamin) biosynthetic enzyme (CobZ) from Rhodobacter capsulatus, containing flavin, heme, and Fe-S cofactors
    Helen M McGoldrick
    School of Biological Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom
    J Biol Chem 280:1086-94. 2005
    ..A mechanism for Orf663 (CobZ) in cobalamin biosynthesis is proposed...
  47. ncbi request reprint Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI
    Rachel J Lawson
    Department of Biochemistry, University of Leicester, UK
    Biochemistry 43:12390-409. 2004
    ..YkuN and YkuP supported monohydroxylation of lauric acid and myristic acid, but secondary oxygenation of the primary product was observed with both palmitic acid and palmitoleic acid as substrates...
  48. ncbi request reprint Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395
    Adrian J Dunford
    Department of Biochemistry, University of Leicester, UK
    Eur J Biochem 271:2548-60. 2004
    ..In wild-type and F1395W FAD domains, prolonged incubation with NADPH results in development of the neutral blue semiquinone FAD species. This reaction is suppressed in the mutant FAD domains lacking the shielding aromatic residue...
  49. ncbi request reprint Thermodynamic basis of electron transfer in dihydroorotate dehydrogenase B from Lactococcus lactis: analysis by potentiometry, EPR spectroscopy, and ENDOR spectroscopy
    Al Walid A Mohsen
    Department of Biochemistry, University of Leicester, UK
    Biochemistry 43:6498-510. 2004
    ..From potentiometric studies, the reduction of the 2Fe-2S center and the reduction of the FMN occur concomitantly. The study provides a detailed thermodynamic framework for electron transfer in this complex iron-sulfur flavoprotein...
  50. ncbi request reprint Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degradation
    Huma Khan
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 279:30563-72. 2004
    ..Implications for the mechanism of high explosive degradation by PETN reductase are discussed...
  51. ncbi request reprint Catalytically functional flavocytochrome chimeras of P450 BM3 and nitric oxide synthase
    Shigeyoshu Fuziwara
    Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan
    J Inorg Biochem 91:515-26. 2002
    ..The data reinforce the importance of the nature of inter-domain linker constitution in multi-domain enzymes, and the difficulties posed in attempts to create chimeric enzymes with enhanced catalytic properties...
  52. ncbi request reprint Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium
    Mattias C U Gustafsson
    Department of Cell and Organism Biology, Lund University, S├Âlvegatan 35, SE 223, 62 Lund, Sweden
    Biochemistry 43:5474-87. 2004
    ..subtilis homologues, pointing to differences in the binding modes for the substrates that may explain the unusual sigmoidal kinetic and titration properties...
  53. ncbi request reprint The TB structural genomics consortium: providing a structural foundation for drug discovery
    Celia W Goulding
    Bioscience Division, Mail Stop M888, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
    Curr Drug Targets Infect Disord 2:121-41. 2002
    ..The Consortium has determined structures of 28 proteins from TB to date. These protein structures are already providing a basis for drug discovery efforts...
  54. doi request reprint Identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis
    Andrew D Lawrence
    Protein Science Group, Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
    J Biol Chem 283:10813-21. 2008
    ..Kinetic and EPR analysis reveals that the enzyme proceeds via a semiquinone form. It is proposed that CobR may interact with the adenosyltransferase to overcome the large thermodynamic barrier required for co(II)rrin reduction...
  55. doi request reprint Biochemical and structural insights into bacterial organelle form and biogenesis
    Joshua B Parsons
    Protein Science Group, Department of Biochemistry, University of Kent, Canterbury, Kent, UK
    J Biol Chem 283:14366-75. 2008
    ..The recombinant model will facilitate future experiments establishing the structure and assembly of these multiprotein assemblages and their fate when the specific metabolic function is no longer required...
  56. ncbi request reprint The redox properties of ascorbate peroxidase
    Igor Efimov
    Department of Chemistry, University of Leicester, University Road, Leicester, LE1 7RH, United Kingdom
    Biochemistry 46:8017-23. 2007
    ....
  57. ncbi request reprint Bacterial flavodoxins support nitric oxide production by Bacillus subtilis nitric-oxide synthase
    Zhi Qiang Wang
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 282:2196-202. 2007
    ..Our results establish YkuN as a kinetically competent redox partner for bsNOS and suggest that FLDR/flavodoxin proteins could function physiologically to support catalysis by bacterial NOSs...
  58. ncbi request reprint Kinetic and thermodynamic characterization of the common polymorphic variants of human methionine synthase reductase
    Horatiu Olteanu
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 43:1988-97. 2004
    ..It is likely that differences in their relative affinities for the redox partner, methionine synthase, underlie the differences in the relative efficiencies of reductive activation exhibited by the variants...
  59. ncbi request reprint Redox and spectroscopic properties of human indoleamine 2,3-dioxygenase and a His303Ala variant: implications for catalysis
    Nektaria D Papadopoulou
    Department of Chemistry, University of Leicester, University Road, Leicester, LE1 7RH, United Kingdom
    Biochemistry 44:14318-28. 2005
    ..The wider implications of these results are discussed in the context of our current understanding of the catalytic mechanism of the enzyme...
  60. ncbi request reprint Interaction of nitric oxide with cytochrome P450 BM3
    Luca G Quaroni
    Elettra Sincrotrone Trieste, 34012 Basovizza, Trieste, Italy
    Biochemistry 43:16416-31. 2004
    ..Nitration of tyrosine in P450 BM3 may exemplify a typical mechanism by which the ubiquitous messenger molecule nitric oxide exerts a regulatory function over the cytochromes P450...
  61. ncbi request reprint Thermodynamic and biophysical characterization of cytochrome P450 BioI from Bacillus subtilis
    Rachel J Lawson
    Department of Biochemistry, University of Leicester, UK
    Biochemistry 43:12410-26. 2004
    ..Calorimetry shows that structural stability of the protein was altered by addition of the reductant dithiothreitol, suggesting that the disulfide is important to integrity of BioI structure...
  62. ncbi request reprint Identification and characterization of the terminal enzyme of siroheme biosynthesis from Arabidopsis thaliana: a plastid-located sirohydrochlorin ferrochelatase containing a 2FE-2S center
    Evelyne Raux-Deery
    School of Biological Sciences, Queen Mary, University of London, Mile End Rd, London E1 4NS, United Kingdom
    J Biol Chem 280:4713-21. 2005
    ..This is the first Fe-S center to be reported in a higher plant ferrochelatase. The implications of the Fe-S center in an enzyme that is so closely associated with the metabolism of sulfur and iron are discussed...
  63. ncbi request reprint A stable tyrosyl radical in monoamine oxidase A
    Stephen E J Rigby
    Department of Biological Sciences, Queen Mary College, University of London, London E1 4NS, United Kingdom
    J Biol Chem 280:4627-31. 2005
    ..The identified tyrosyl radical provides the key missing link in support of the single electron transfer mechanism for amine oxidation by MAO enzymes...