Nigel P Minton

Summary

Affiliation: University of Nottingham
Country: UK

Publications

  1. doi request reprint A modular system for Clostridium shuttle plasmids
    John T Heap
    BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Microbiol Methods 78:79-85. 2009
  2. pmc The analysis of para-cresol production and tolerance in Clostridium difficile 027 and 012 strains
    Lisa F Dawson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    BMC Microbiol 11:86. 2011
  3. pmc A roadmap for gene system development in Clostridium
    Nigel P Minton
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre, School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD, UK Nottingham Digestive Disease Centre, NIHR Biomedical Research Unit, The University of Nottingham, University Park, Nottingham, UK Electronic address
    Anaerobe 41:104-112. 2016
  4. pmc Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824
    Katalin Kovacs
    Clostridia Research Group, BBSRC Sustainable BioEnergy Centre, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Biotechnol Biofuels 6:117. 2013
  5. ncbi request reprint The development of Clostridium difficile genetic systems
    Nigel Minton
    Institute of Infection, Immunity and Inflammation, University of Nottingham, Floor C, West Block, Queens Medical Centre, Nottingham NG7 2UH, UK
    Anaerobe 10:75-84. 2004
  6. ncbi request reprint Clostridia in cancer therapy
    Nigel P Minton
    Institute of Infections, Immunity and Inflammation, School of Pharmacy, University of Nottingham, Floor C, West Block, Queens Medical Centre, Nottingham NG7 2UH, UK
    Nat Rev Microbiol 1:237-42. 2003
  7. pmc Importance of toxin A, toxin B, and CDT in virulence of an epidemic Clostridium difficile strain
    Sarah A Kuehne
    Clostridia Research Group, Nottingham Digestive Diseases Centre, NIHR Biomedical Research Unit, School of Life Sciences, University of Nottingham, United Kingdom
    J Infect Dis 209:83-6. 2014
  8. pmc Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
    Michelle L Kelly
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom
    Anaerobe 39:51-3. 2016
  9. pmc Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production
    Stephen T Cartman
    Clostridia Research Group, Centre for Biomolecular Sciences, School of Molecular Medical Sciences, Nottingham Digestive Diseases Centre, NIHR Biomedical Research Unit, The University of Nottingham, University Park, United Kingdom
    Appl Environ Microbiol 78:4683-90. 2012
  10. pmc Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles
    Yen Kuan Ng
    Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 8:e56051. 2013

Collaborators

Detail Information

Publications51

  1. doi request reprint A modular system for Clostridium shuttle plasmids
    John T Heap
    BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Microbiol Methods 78:79-85. 2009
    ..We propose that adoption of this modular system as a standard would be of substantial benefit to the Clostridium research community, whom we invite to use and contribute to the system...
  2. pmc The analysis of para-cresol production and tolerance in Clostridium difficile 027 and 012 strains
    Lisa F Dawson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    BMC Microbiol 11:86. 2011
    ..It has been proposed that the hpdBCA operon, rarely found in other gut microflora, encodes the enzymes responsible for the conversion of p-HPA to p-cresol...
  3. pmc A roadmap for gene system development in Clostridium
    Nigel P Minton
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre, School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD, UK Nottingham Digestive Disease Centre, NIHR Biomedical Research Unit, The University of Nottingham, University Park, Nottingham, UK Electronic address
    Anaerobe 41:104-112. 2016
    ....
  4. pmc Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824
    Katalin Kovacs
    Clostridia Research Group, BBSRC Sustainable BioEnergy Centre, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Biotechnol Biofuels 6:117. 2013
    ....
  5. ncbi request reprint The development of Clostridium difficile genetic systems
    Nigel Minton
    Institute of Infection, Immunity and Inflammation, University of Nottingham, Floor C, West Block, Queens Medical Centre, Nottingham NG7 2UH, UK
    Anaerobe 10:75-84. 2004
    ..This efficient transfer can only be achieved in certain strains through negation of the indigenous restriction barrier, and is generally most effective when the plasmid employed is based on the replicon of the C. difficile plasmid, pCD6...
  6. ncbi request reprint Clostridia in cancer therapy
    Nigel P Minton
    Institute of Infections, Immunity and Inflammation, School of Pharmacy, University of Nottingham, Floor C, West Block, Queens Medical Centre, Nottingham NG7 2UH, UK
    Nat Rev Microbiol 1:237-42. 2003
    ....
  7. pmc Importance of toxin A, toxin B, and CDT in virulence of an epidemic Clostridium difficile strain
    Sarah A Kuehne
    Clostridia Research Group, Nottingham Digestive Diseases Centre, NIHR Biomedical Research Unit, School of Life Sciences, University of Nottingham, United Kingdom
    J Infect Dis 209:83-6. 2014
    ..We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence. ..
  8. pmc Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection
    Michelle L Kelly
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom
    Anaerobe 39:51-3. 2016
    ..Mutants can appear more virulent. We have rectified this anomaly by genome engineering. The variant created (CRG20291) represents an ideal control strain for virulence assays of ClosTron mutants. ..
  9. pmc Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production
    Stephen T Cartman
    Clostridia Research Group, Centre for Biomolecular Sciences, School of Molecular Medical Sciences, Nottingham Digestive Diseases Centre, NIHR Biomedical Research Unit, The University of Nottingham, University Park, United Kingdom
    Appl Environ Microbiol 78:4683-90. 2012
    ..This may well explain why several studies have reported that an aberrant tcdC gene does not predict increased toxin production or, indeed, increased virulence...
  10. pmc Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles
    Yen Kuan Ng
    Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 8:e56051. 2013
    ..The rapidity of the 'correction' method (5-7 days) makes pyrE(-) strains attractive hosts for mutagenesis studies...
  11. doi request reprint ClosTron-targeted mutagenesis
    John T Heap
    Centre for Biomolecular Sciences, Institute of Infection Immunity and Inflammation, BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, University of Nottingham, Nottingham, UK
    Methods Mol Biol 646:165-82. 2010
    ..Furthermore, the insertions made are extremely stable. Its deployment has considerably expanded available options for clostridial functional genomic studies...
  12. pmc Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker
    John T Heap
    Clostridia Research Group, BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, The University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Nucleic Acids Res 40:e59. 2012
    ..acetobutylicum in three steps. This work should open the way to reliable integration of DNA including large synthetic constructs in diverse microorganisms...
  13. pmc The role of flagella in Clostridium difficile pathogenesis: comparison between a non-epidemic and an epidemic strain
    Soza T Baban
    Clostridia Research Group, NIHR Biomedical Research Unit in GI Disease, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 8:e73026. 2013
    ..difficile strains. The latter emphasises the overriding need to characterize more than just one strain before drawing general conclusions concerning specific mechanisms of pathogenesis. ..
  14. pmc Regulation of neurotoxin production and sporulation by a Putative agrBD signaling system in proteolytic Clostridium botulinum
    Clare M Cooksley
    Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
    Appl Environ Microbiol 76:4448-60. 2010
    ..botulinum, also reduced production of neurotoxin, suggesting that both phenotypes are controlled by quorum sensing. Interestingly, while agr-1 appeared to control sporulation, agr-2 appeared to regulate neurotoxin formation...
  15. doi request reprint The emergence of 'hypervirulence' in Clostridium difficile
    Stephen T Cartman
    Centre for Biomolecular Sciences, School of Molecular Medical Sciences, Nottingham Digestive Diseases Centre NIHR Biomedical Research Unit, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Int J Med Microbiol 300:387-95. 2010
    ..The identification of virulence factors using this approach should help lead to the rational development of therapeutic countermeasures against CDAD...
  16. doi request reprint The ClosTron: Mutagenesis in Clostridium refined and streamlined
    John T Heap
    BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Microbiol Methods 80:49-55. 2010
    ..The improved ClosTron system supersedes the prototype plasmid pMTL007 and the original method, and exploits the potential of Group II introns more fully...
  17. doi request reprint The role of toxin A and toxin B in Clostridium difficile infection
    Sarah A Kuehne
    Clostridia Research Group, Centre for Biomolecular Sciences, School of Molecular Medical Sciences, Nottingham Digestive Diseases Centre, NIHR Biomedical Research Unit, University of Nottingham, Nottingham NG7 2RD, UK
    Nature 467:711-3. 2010
    ..Our findings re-establish the importance of both toxin A and toxin B and highlight the need to continue to consider both toxins in the development of diagnostic tests and effective countermeasures against C. difficile...
  18. doi request reprint ClosTron-mediated engineering of Clostridium
    Sarah A Kuehne
    Clostridia Research Group, BBSRC Sustainable BioEnergy Centre, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, UK
    Methods Mol Biol 765:389-407. 2011
    ..The procedure is extremely efficient, rapid, and requires minimal effort by the operator...
  19. pmc Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts
    Daniela Heeg
    Clostridia Research Group, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 7:e32381. 2012
    ..Furthermore, we stress the importance of studying multiple isolates in the future when analysing the nutrients or chemicals that either stimulate or inhibit C. difficile spore germination...
  20. pmc A genetic assay for gene essentiality in Clostridium
    David J F Walker
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom
    Anaerobe 42:40-43. 2016
    ..Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene...
  21. pmc ClosTron-mediated engineering of Clostridium
    Sarah A Kuehne
    Clostridia Research Group, BBSRC Sustainable BioEnergy Centre, NIHR Biomedical Research Unit in GI Disease, University of Nottingham, Nottingham, UK
    Bioengineered 3:247-54. 2012
    ..Re-targeted ClosTrons are delivered ready for use in 10-14 days, allowing mutants to be isolated 5-7 days after receipt. Its availability has revolutionized clostridial molecular biology...
  22. pmc Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824
    Muhammad Ehsaan
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, University of Nottingham, University Park, Nottingham, NG7 2RD UK
    Biotechnol Biofuels 9:4. 2016
    ..In the current study we sought to test their suitability for use in C. acetobutylicum...
  23. pmc Reconsidering the sporulation characteristics of hypervirulent Clostridium difficile BI/NAP1/027
    David A Burns
    Clostridia Research Group, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 6:e24894. 2011
    ..Furthermore, we stress the need for more rigorous experimental procedures in order to quantify C. difficile sporulation more accurately in the future...
  24. pmc Array comparative hybridisation reveals a high degree of similarity between UK and European clinical isolates of hypervirulent Clostridium difficile
    Gemma L Marsden
    Centre for Biomolecular Sciences, School of Molecular Medical Sciences, Nottingham Digestive Diseases Centre NIHR Biomedical Research, University of Nottingham, Nottingham NG7 2RD, UK
    BMC Genomics 11:389. 2010
    ..The aim of this work was to identify regions of sequence divergence that may be used as genetic markers of hypervirulent PCR-ribotype 027 strains and markers of the sequenced strain, CD630 by array comparative hybridisation...
  25. pmc SleC is essential for germination of Clostridium difficile spores in nutrient-rich medium supplemented with the bile salt taurocholate
    David A Burns
    Centre for Biomolecular Sciences, School of Molecular Medical Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    J Bacteriol 192:657-64. 2010
    ..Furthermore, fewer R20291 spores germinated, indicating that there are differences in both sporulation and germination between these epidemic and nonepidemic C. difficile isolates...
  26. ncbi request reprint What's a SNP between friends: The influence of single nucleotide polymorphisms on virulence and phenotypes of Clostridium difficile strain 630 and derivatives
    Mark M Collery
    a Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Center SBRC, School of Life Sciences, University of Nottingham, Nottingham, UK
    Virulence . 2016
    ..They also underline the importance of effective strain curation and the need to genome re-sequence master seed banks wherever possible...
  27. pmc Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis
    Fungmin Liew
    BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, University Park, The University of Nottingham, Nottingham, United Kingdom LanzaTech Inc, Skokie, Illinois, USA
    MBio 7:. 2016
    ..Insights gained from studying the function of CODH enhance the overall understanding of autotrophy and can be used for optimization of biotechnological production of ethanol and other commodities via gas fermentation...
  28. doi request reprint The diverse sporulation characteristics of Clostridium difficile clinical isolates are not associated with type
    David A Burns
    Nottingham Digestive Diseases Centre NIHR Biomedical Research Unit, Centre for Biomolecular Sciences, University of Nottingham, University Park, UK
    Anaerobe 16:618-22. 2010
    ..The data suggest that (i) careful experimental design is required in order to accurately quantify sporulation; and (ii) current evidence cannot link differences in sporulation rates with the disease severity of the BI/NAP1/027 type...
  29. pmc Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
    Benjamin J Willson
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre, School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD UK
    Biotechnol Biofuels 9:109. 2016
    ..thermocellum-derived minicellulosome by recombinant strains of C. acetobutylicum, and aim to develop on this success, addressing potential issues with the previous strategy...
  30. pmc A mariner-based transposon system for in vivo random mutagenesis of Clostridium difficile
    Stephen T Cartman
    Centre for Biomolecular Sciences, School of Molecular Medical Sciences, Nottingham Digestive Diseases Centre, NIHR Biomedical Research Unit, The University of Nottingham, University Park, Nottingham NG72RD, United Kingdom
    Appl Environ Microbiol 76:1103-9. 2010
    ..These results validate our mariner-based transposon system for use in forward genetic studies of C. difficile...
  31. pmc Metabolic Engineering of Clostridium autoethanogenum for Selective Alcohol Production
    Fungmin Liew
    BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, University Park, The University of Nottingham, Nottingham, NG7 2RD, UK LanzaTech Inc, 8045 Lamon Avenue, Suite 400, Skokie, IL, USA
    Metab Eng . 2017
    ....
  32. doi request reprint The role of small acid-soluble proteins (SASPs) in protection of spores of Clostridium botulinum against nitrous acid
    Carolyn A Meaney
    School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Int J Food Microbiol 216:25-30. 2016
    ..These data indicate that the SASPs CBO1789 or CBO1790 play a significant role in resistance to nitrous acid, but not in resistance to formaldehyde or hydrogen peroxide. ..
  33. doi request reprint Clostridium difficile spore germination: an update
    David A Burns
    Nottingham Digestive Diseases Centre NIHR Biomedical Research Unit NDDC BRU, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Res Microbiol 161:730-4. 2010
    ..difficile spores using both a forward and reverse genetics approach. Significant progress is beginning to be made in the study of this important aspect of C. difficile disease...
  34. doi request reprint Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway
    Clare M Cooksley
    Clostridia Research Group, BBSRC Sustainable BioEnergy Centre, School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Metab Eng 14:630-41. 2012
    ..This finding reinforces the need for mutant complementation and Southern Blot analysis (to confirm single ClosTron insertions), which should be obligatory in all further ClosTron applications...
  35. pmc Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum
    Katrin M Schwarz
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Metab Eng . 2017
    ..Inactivation of both rex and hydA resulted in increased n-butanol titres, representing the first steps towards improving the utilisation of C. pasteurianum as a chassis for the industrial production of this important chemical...
  36. doi request reprint The role of flagella in Clostridium difficile pathogenicity
    Emma Stevenson
    Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK Electronic address
    Trends Microbiol 23:275-82. 2015
    ..difficile virulence pertaining to the regulation of toxin gene expression. This review focuses on new insights into the specific role of C. difficile flagella in colonisation and toxin gene expression. ..
  37. pmc Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain
    Lili Sheng
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, United Kingdom
    Genome Announc 4:. 2016
    ..Here, we present the genome sequence of strain NCIMB 11955, the progenitor of an ethanologenic industrial strain, revealing 11 single-nucleotide polymorphisms and 2 indels compared to strain DSM 2542 and two novel plasmids. ..
  38. doi request reprint Clostridium difficile Genome Editing Using pyrE Alleles
    Muhammad Ehsaan
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Methods Mol Biol 1476:35-52. 2016
    ..This avoids the phenotypic effects frequently observed with high-copy-number plasmids and dispenses with the need to add antibiotic to ensure plasmid retention. ..
  39. doi request reprint Comparison of culture based methods for the isolation of Clostridium difficile from stool samples in a research setting
    Michelle Lister
    Clostridia Research Group, Centre for Biomolecular Sciences, School of Life Sciences, Nottingham Digestive Diseases Centre, NIHR Biomedical Research Unit, The University of Nottingham, Nottingham, United Kingdom
    Anaerobe 28:226-9. 2014
    ..For a low cost, timely and sensitive method of isolating C. difficile from stool samples we recommend direct plating onto CCEY egg yolk agar after heat shock. ..
  40. ncbi request reprint Quorum sensing in Clostridium difficile: analysis of a luxS-type signalling system
    Glen P Carter
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    J Med Microbiol 54:119-27. 2005
    ..RolA, therefore, acts as a negative regulator of AI-2 production. Finally, it has been shown that the exogenous addition of AI-2 or 4-hydroxy-5-methyl-3(2H) furanone has no discernible effect on the production of toxins by C. difficile...
  41. pmc Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo
    John T Heap
    Clostridia Research Group, Centre for Biomolecular Sciences, School of Life Sciences, The University of Nottingham, University Park, Nottingham, UK
    Oncotarget 5:1761-9. 2014
    ..Substantial tumor suppression was achieved, and several animals were cured. These encouraging data suggest that the novel enzyme and strain engineering approach represent a promising platform for the clinical development of CDEPT. ..
  42. doi request reprint Sporulation studies in Clostridium difficile
    David A Burns
    Nottingham Digestive Diseases Centre NIHR Biomedical Research Unit, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Microbiol Methods 87:133-8. 2011
    ..Specifically, the advantages and disadvantages of the different experimental approaches previously used are discussed and a standard set of principles for measuring C. difficile sporulation in the future is proposed...
  43. pmc Both, toxin A and toxin B, are important in Clostridium difficile infection
    Sarah A Kuehne
    Clostridia Research Group, Centre for Biomolecular Sciences, School of Molecular Medical Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Gut Microbes 2:252-5. 2011
    ..In this article we discuss our findings in the context of previous work and outline some of the challenges which face the field as a result...
  44. doi request reprint Optimal spore germination in Clostridium botulinum ATCC 3502 requires the presence of functional copies of SleB and YpeB, but not CwlJ
    Carolyn A Meaney
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre, School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Anaerobe 34:86-93. 2015
    ..Taken together, these data indicate that functional copies of SleB and YpeB, but not CwlJ are required for the optimal germination of the spores of C. botulinum ATCC 3502. ..
  45. pmc Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection
    Emma C Stevenson
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
    J Clin Microbiol 54:2689-2694. 2016
    ..This study has been registered at ClinicalTrials.gov under registration no. NCT01670149.)...
  46. pmc Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum
    Ying Zhang
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    FEMS Microbiol Lett 363:. 2016
    ..Phenotypic screening of 200 transposon clones revealed a sporulation-defective clone with an insertion in spo0A, thereby demonstrating that this inducible transposon system can be used for forward genetic studies in C. acetobutylicum. ..
  47. pmc Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium
    Christopher M Humphreys
    BBSRC EPSRC Synthetic Biology Research Centre, School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD, UK
    BMC Genomics 16:1085. 2015
    ..However, crucial to metabolic modelling and directed pathway engineering is a reliable and comprehensively annotated genome sequence...
  48. pmc A universal mariner transposon system for forward genetic studies in the genus Clostridium
    Ying Zhang
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 10:e0122411. 2015
    ..Moreover, appropriate screening of both libraries resulted in the isolation of auxotrophic mutants as well as cells deficient in spore formation/germination. This strategy is capable of being implemented in any Clostridium species. ..
  49. ncbi request reprint The ClosTron: a universal gene knock-out system for the genus Clostridium
    John T Heap
    Institute of Infection, Immunity and Inflammation, School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    J Microbiol Methods 70:452-64. 2007
    ..The procedure is highly efficient and reproducible, and should revolutionize functional genomic studies in clostridia...
  50. doi request reprint The potential of clostridial spores as therapeutic delivery vehicles in tumour therapy
    Aleksandra M Kubiak
    Clostridia Research Group, BBSRC EPSRC Synthetic Biology Research Centre SBRC, School of Life Sciences, The University of Nottingham, Nottingham NG7 2RD, UK
    Res Microbiol 166:244-54. 2015
    ..Much of this progress has been due to advances in genomics and our ability to modify strains using more sophisticated gene tools. ..
  51. ncbi request reprint Bacillus amyloliquefaciens orthologue of Bacillus subtilis ywrO encodes a nitroreductase enzyme which activates the prodrug CB 1954
    Gill M Anlezark
    Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, UK
    Microbiology 148:297-306. 2002
    ..amyloliquefaciens YwrO, with distinct properties which will aid the rational selection of appropriate genes for applications in directed enzyme prodrug therapy (DEPT)...