Mario Juhas

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. doi request reprint Integrative bacterial artificial chromosomes for DNA integration into the Bacillus subtilis chromosome
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP Cambridge, UK Electronic address
    J Microbiol Methods 125:1-7. 2016
  2. pmc Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Microb Cell Fact 15:172. 2016
  3. doi request reprint Type IV secretion systems and genomic islands-mediated horizontal gene transfer in Pseudomonas and Haemophilus
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP Cambridge, UK Electronic address
    Microbiol Res 170:10-7. 2015
  4. doi request reprint Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Microbiology 160:2341-51. 2014
  5. pmc Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, NDCLS, University of Oxford, OX3 9DU, UK
    Plasmid 70:277-83. 2013
  6. pmc Genomic islands: tools of bacterial horizontal gene transfer and evolution
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford, UK
    FEMS Microbiol Rev 33:376-93. 2009
  7. pmc Sequence and functional analyses of Haemophilus spp. genomic islands
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, NDCLS, University of Oxford, Headley Way, Oxford OX3 9DU, UK
    Genome Biol 8:R237. 2007
  8. pmc Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, UK
    Cell Microbiol 10:2377-86. 2008
  9. pmc Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae
    Esther Robinson
    Nuffield Department of Clinical Laboratory Sciences, University of Oxford, UK
    J Microbiol Methods 83:330-4. 2010
  10. pmc Escherichia coli flagellar genes as target sites for integration and expression of genetic circuits
    Mario Juhas
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 9:e111451. 2014

Detail Information

Publications15

  1. doi request reprint Integrative bacterial artificial chromosomes for DNA integration into the Bacillus subtilis chromosome
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP Cambridge, UK Electronic address
    J Microbiol Methods 125:1-7. 2016
    ..coli K12 MG1655 into B. subtilis chromosome. iBAC(cav)-mediated chromosomal integration approach will facilitate rational design of synthetic biology applications in B. subtilis. ..
  2. pmc Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Microb Cell Fact 15:172. 2016
    ..Novel approaches for integrating high molecular weight DNA into the E. coli chromosome would therefore greatly facilitate engineering efforts in this bacterium...
  3. doi request reprint Type IV secretion systems and genomic islands-mediated horizontal gene transfer in Pseudomonas and Haemophilus
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP Cambridge, UK Electronic address
    Microbiol Res 170:10-7. 2015
    ..It highlights the role T4SSs play in the horizontal gene transfer, particularly in the evolution of catabolic pathways, antibiotic-resistance and virulence in Haemophilus and Pseudomonas. ..
  4. doi request reprint Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Microbiology 160:2341-51. 2014
    ..Special attention is focused on the genome reduction analyses in B. subtilis and E. coli and the construction of minimal cell factories for industrial applications...
  5. pmc Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, NDCLS, University of Oxford, OX3 9DU, UK
    Plasmid 70:277-83. 2013
    ....
  6. pmc Genomic islands: tools of bacterial horizontal gene transfer and evolution
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford, UK
    FEMS Microbiol Rev 33:376-93. 2009
    ..Depending on the composition of gene modules, the same type of GEIs can promote survival of pathogenic as well as environmental bacteria...
  7. pmc Sequence and functional analyses of Haemophilus spp. genomic islands
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, NDCLS, University of Oxford, Headley Way, Oxford OX3 9DU, UK
    Genome Biol 8:R237. 2007
    ..More clarity comes from this comparative analysis of seven complete sequences of the ICEHin1056 genomic island subfamily...
  8. pmc Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, UK
    Cell Microbiol 10:2377-86. 2008
    ..The most recently described GI type T4SSs play a key role in the horizontal transfer of a wide variety of genomic islands derived from a broad spectrum of bacterial strains...
  9. pmc Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae
    Esther Robinson
    Nuffield Department of Clinical Laboratory Sciences, University of Oxford, UK
    J Microbiol Methods 83:330-4. 2010
    ..influenzae and is functional for allelic replacement and mutant complementation. The vector will be useful for investigating gene function in Haemophilus spp...
  10. pmc Escherichia coli flagellar genes as target sites for integration and expression of genetic circuits
    Mario Juhas
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 9:e111451. 2014
    ..None of the integrations had negative effects on the growth of the bacteria. Overall, we found that fliT was the most suitable integration site. ..
  11. pmc Identification and validation of novel chromosomal integration and expression loci in Escherichia coli flagellar region 1
    Mario Juhas
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 10:e0123007. 2015
    ..From the investigated E. coli K12 MG1655 flagellar region 1, flgA and flgG are the most suitable chromosomal integration and expression loci. ..
  12. pmc Flagellar region 3b supports strong expression of integrated DNA and the highest chromosomal integration efficiency of the Escherichia coli flagellar regions
    Mario Juhas
    Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP, Cambridge, UK
    Microb Biotechnol 8:726-38. 2015
    ..coli K12 MG1655 flagellar regions and is therefore potentially the most suitable for the integration of synthetic genetic circuits. ..
  13. ncbi request reprint High molecular weight DNA assembly in vivo for synthetic biology applications
    Mario Juhas
    a Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Crit Rev Biotechnol . 2016
    ..coli, B. subtilis and S. cerevisiae. Special attention is paid to the assembly of whole genomes, such as those of the first synthetic cell, synthetic yeast and minimal genomes...
  14. pmc Combining Genes from Multiple Phages for Improved Cell Lysis and DNA Transfer from Escherichia coli to Bacillus subtilis
    Mario Juhas
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 11:e0165778. 2016
    ..coli to B. subtilis. Our engineered system combines benefits of the two main synthetic biology chassis, E. coli and B. subtilis, and allows reliable and efficient transfer of DNA edited in E. coli into B. subtilis...
  15. pmc Novel type IV secretion system involved in propagation of genomic islands
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Headington, OX3 9DU Oxford, United Kingdom
    J Bacteriol 189:761-71. 2007
    ..Moreover, the expression of this T4SS was found to be positively regulated by one of its components, the tfc24 gene. We concluded that this gene cluster represents a novel family of T4SSs involved in propagation of genomic islands...