G J Jenkins

Summary

Affiliation: University of Wales
Country: UK

Publications

  1. ncbi request reprint Mutation analysis using the restriction site mutation (RSM) assay
    G J Jenkins
    School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, UK
    Mutat Res 405:209-20. 1998
  2. doi request reprint Genotoxic thresholds, DNA repair, and susceptibility in human populations
    Gareth J S Jenkins
    Institute of Life Science, Swansea School of Medicine, Swansea University, Singleton Park, Swansea SA28PP, United Kingdom
    Toxicology 278:305-10. 2010
  3. doi request reprint The bile acid deoxycholic acid has a non-linear dose response for DNA damage and possibly NF-kappaB activation in oesophageal cells, with a mechanism of action involving ROS
    G J S Jenkins
    Institute of Life Science, Swansea School of Medicine, Swansea University, Swansea SA2 8PP, UK
    Mutagenesis 23:399-405. 2008
  4. pmc Immunohistochemical study of nuclear factor-kappaB activity and interleukin-8 abundance in oesophageal adenocarcinoma; a useful strategy for monitoring these biomarkers
    G J S Jenkins
    Swansea School of Medicine, University of Wales Swansea, Swansea, UK
    J Clin Pathol 60:1232-7. 2007
  5. ncbi request reprint Deoxycholic acid at neutral and acid pH, is genotoxic to oesophageal cells through the induction of ROS: The potential role of anti-oxidants in Barrett's oesophagus
    Gareth J S Jenkins
    Swansea School of Medicine, Swansea University, Swansea SA28PP, UK
    Carcinogenesis 28:136-42. 2007
  6. ncbi request reprint The restriction site mutation (RSM) method: clinical applications
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, Singleton Park, Swansea SA28PP, UK
    Mutagenesis 19:3-11. 2004
  7. ncbi request reprint The bile acid deoxycholic acid (DCA) at neutral pH activates NF-kappaB and induces IL-8 expression in oesophageal cells in vitro
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, Swansea, Wales, UK
    Carcinogenesis 25:317-23. 2004
  8. pmc Early p53 mutations in nondysplastic Barrett's tissue detected by the restriction site mutation (RSM) methodology
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, UK
    Br J Cancer 88:1271-6. 2003
  9. ncbi request reprint Molecular analysis of the chemoprotective effects of topical sunscreen and vitamin C in preventing UV-induced and reactive oxygen species-induced DNA damage, respectively, using the PCR inhibition methodology
    G J S Jenkins
    School of Biological Sciences, University of Wales Swansea, Swansea SA28PP, U K
    Anticancer Res 22:3873-7. 2002
  10. ncbi request reprint Genetic pathways involved in the progression of Barrett's metaplasia to adenocarcinoma
    G J S Jenkins
    Human Molecular Pathology Group, Swansea Clinical School, University of Wales Swansea, UK
    Br J Surg 89:824-37. 2002

Detail Information

Publications30

  1. ncbi request reprint Mutation analysis using the restriction site mutation (RSM) assay
    G J Jenkins
    School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, UK
    Mutat Res 405:209-20. 1998
    ..A later mutational peak, after the adduct level had declined, was assumed to be due to DNA sequence changes produced in the fibroblasts by the in vivo processing of DNA adducts...
  2. doi request reprint Genotoxic thresholds, DNA repair, and susceptibility in human populations
    Gareth J S Jenkins
    Institute of Life Science, Swansea School of Medicine, Swansea University, Singleton Park, Swansea SA28PP, United Kingdom
    Toxicology 278:305-10. 2010
    ..By studying polymorphisms in DNA repair genes, susceptible individuals may be identified, and additional safety factors appropriately targeted to these populations...
  3. doi request reprint The bile acid deoxycholic acid has a non-linear dose response for DNA damage and possibly NF-kappaB activation in oesophageal cells, with a mechanism of action involving ROS
    G J S Jenkins
    Institute of Life Science, Swansea School of Medicine, Swansea University, Swansea SA2 8PP, UK
    Mutagenesis 23:399-405. 2008
    ..Either way, antioxidant supplementation may possibly help prevent the deleterious effects of DCA in the whole GI tract...
  4. pmc Immunohistochemical study of nuclear factor-kappaB activity and interleukin-8 abundance in oesophageal adenocarcinoma; a useful strategy for monitoring these biomarkers
    G J S Jenkins
    Swansea School of Medicine, University of Wales Swansea, Swansea, UK
    J Clin Pathol 60:1232-7. 2007
    ..The pro-inflammatory cytokine interleukin-8 (IL-8), a transcriptional target of NF-kappaB, was also studied to better understand NF-kappaB functionality; its RNA and protein levels were assessed in oesophageal tissues...
  5. ncbi request reprint Deoxycholic acid at neutral and acid pH, is genotoxic to oesophageal cells through the induction of ROS: The potential role of anti-oxidants in Barrett's oesophagus
    Gareth J S Jenkins
    Swansea School of Medicine, Swansea University, Swansea SA28PP, UK
    Carcinogenesis 28:136-42. 2007
    ..The genotoxicity of DCA is however, ROS dependent, hence anti-oxidant supplementation, in addition to acid suppression may block DCA driven carcinogenesis in Barrett's patients...
  6. ncbi request reprint The restriction site mutation (RSM) method: clinical applications
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, Singleton Park, Swansea SA28PP, UK
    Mutagenesis 19:3-11. 2004
    ..We can also use these clinical mutation data to speculate as to the causative mutagens involved in these cancer conditions. We here use an example of mutations detected in gastric tissue and those induced in vitro by hydrogen peroxide...
  7. ncbi request reprint The bile acid deoxycholic acid (DCA) at neutral pH activates NF-kappaB and induces IL-8 expression in oesophageal cells in vitro
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, Swansea, Wales, UK
    Carcinogenesis 25:317-23. 2004
    ..Hence chromosome 4 amplification may provide a survival mechanism for bile exposed oesophageal tissues via NF-kappaB...
  8. pmc Early p53 mutations in nondysplastic Barrett's tissue detected by the restriction site mutation (RSM) methodology
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, UK
    Br J Cancer 88:1271-6. 2003
    ..4) and low-grade dysplasia patients (P=0.33) and 45% of high-grade dysplasia patients (P=0.15). Detected p53 mutations were mainly GC to AT transitions at CpG sites...
  9. ncbi request reprint Molecular analysis of the chemoprotective effects of topical sunscreen and vitamin C in preventing UV-induced and reactive oxygen species-induced DNA damage, respectively, using the PCR inhibition methodology
    G J S Jenkins
    School of Biological Sciences, University of Wales Swansea, Swansea SA28PP, U K
    Anticancer Res 22:3873-7. 2002
    ..We wanted to explore the possibility of detecting anti-carcinogens with this methodology through a corresponding reduction in DNA damage induction...
  10. ncbi request reprint Genetic pathways involved in the progression of Barrett's metaplasia to adenocarcinoma
    G J S Jenkins
    Human Molecular Pathology Group, Swansea Clinical School, University of Wales Swansea, UK
    Br J Surg 89:824-37. 2002
    ..The prediction of which patients with Barrett's metaplasia will develop cancer is difficult. Better genetic characterization of the condition may aid clinicians in devising more effective management and follow-up strategies...
  11. ncbi request reprint Restriction enzymes in the analysis of genetic alterations responsible for cancer progression
    G J S Jenkins
    Human Molecular Pathology Group, Swansea Clinical School, University of Wales Swansea, Singleton Park, Swansea, UK
    Br J Surg 89:8-20. 2002
    ..The authors aim to aid interpretation of molecular studies in general by presenting a comprehensive review of one molecular approach, i.e. the use of restriction enzymes in molecular studies of tumour development...
  12. ncbi request reprint A study of ENU-induced mutagenesis in the mouse using the restriction site mutation (RSM) assay
    G J Jenkins
    School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, United Kingdom
    Teratog Carcinog Mutagen 19:281-92. 1999
    ..We also show a comparison of the bone marrow micronucleus data with the RSM assay and show that both assays are capable of detecting the genotoxicity of ENU to the mouse bone marrow in vivo...
  13. ncbi request reprint The detection of mutations induced in vitro in the human p53 gene by hydrogen peroxide with the restriction site mutation (RSM) assay
    G J Jenkins
    Human Molecular Pathology Group, Swansea Clinical School, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK
    Mutat Res 498:135-44. 2001
    ..Hence, this methodology may allow the detection of early p53 mutations in pre-malignant tissues...
  14. ncbi request reprint The restriction site mutation assay: a review of the methodology development and the current status of the technique
    G J Jenkins
    School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK
    Mutagenesis 14:439-48. 1999
    ..Our aim in presenting the developmental data on the RSM assay is to provide other researchers with sufficient information about the RSM methodology to facilitate its application in mutation analysis in other genes and organisms...
  15. ncbi request reprint Inverse restriction site mutation (iRSM) analysis. Mutation detection involving the formation of restriction enzyme sites in target genes
    G J Jenkins
    Centre for Molecular Genetics and Toxicology, University of Wales, Swansea, UK
    Mutagenesis 14:37-42. 1999
    ..The potential application of this method in other genes and organisms as a rapid screen for induced mutations is discussed...
  16. ncbi request reprint PCR-based methodology for the determination of the photoprotection afforded by sunscreen application
    G J Jenkins
    University of Wales Swansea, Swansea, Wales, UK
    Biotechniques 29:1318-20, 1323-6. 2000
    ..This methodology may also be useful in identifying new photoprotective agents by assessing their relative value as UV-B and UV-A absorbing agents...
  17. ncbi request reprint Restriction site mutation (RSM) analysis of 2-acetylaminofluorene (2-AAF)-induced mouse liver mutations and comparison with the measurement of in vivo micronucleus induction in the bone marrows of (2-AAF)-treated mice
    G J Jenkins
    University of Wales Swansea, Singleton Park, Swansea, UK
    Teratog Carcinog Mutagen 20:107-17. 2000
    ..Teratogenesis Carcinog. Mutagen. 20:107-117, 2000...
  18. ncbi request reprint In vitro and in vivo extrapolations of genotoxin exposures: consideration of factors which influence dose-response thresholds
    J M Parry
    Centre for Molecular Genetics and Toxicology, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, UK
    Mutat Res 464:53-63. 2000
    ..Further studies are necessary to evaluate the consequences of the genetic background of tester strains upon the nature of the dose-response curve of aneugenic chemicals...
  19. doi request reprint Diagnostic correlation between the expression of the DNA repair enzyme N-methylpurine DNA glycosylase and esophageal adenocarcinoma onset: a retrospective pilot study
    Z M Zaïr
    Institute of Life Sciences, School of Medicine, Swansea University, Swansea, UK
    Dis Esophagus 26:644-50. 2013
    ..Indeed, disease-stage-specific alterations in the expression of MPG may highlight a potential role for MPG in determining EAC onset and thus potentially be of clinical relevance for early disease detection and increased patient survival...
  20. ncbi request reprint Generation of locus-specific probes for interphase fluorescence in situ hybridisation--application in Barrett's esophagus
    S H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales Swansea, Swansea, SA2 8PP, UK
    Exp Mol Pathol 77:26-33. 2004
    ..No significant alterations at the DNMT1 and DNMT3a loci were detected. An increased copy number of these genes is therefore not the basis for the hypermethylation commonly observed in this premalignant lesion...
  21. pmc Chromosome 4 hyperploidy represents an early genetic aberration in premalignant Barrett's oesophagus
    S H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales, Swansea SA2 8PP, UK
    Gut 52:623-8. 2003
    ..In an attempt to understand these causative pathways, fluorescence in situ hybridisation (FISH) was used in this study to determine when specific genetic alterations arise during Barrett's associated neoplastic progression...
  22. pmc Characterisation of p53 status at the gene, chromosomal and protein levels in oesophageal adenocarcinoma
    S H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales, Swansea SA2 8PP, UK
    Br J Cancer 89:1729-35. 2003
    ..P53 gene mutations are not the primary cause of protein overexpression--an alternative mechanism is responsible for the positive p53 immunohistochemistry detected...
  23. doi request reprint Confounding experimental considerations in nanogenotoxicology
    S H Doak
    Institute of Life Science, Swansea University, Wales, UK
    Mutagenesis 24:285-93. 2009
    ..Thus, highlighting some of the potential confounding factors that need to be considered in order to ensure that in vitro genotoxicity assays report true biological impacts in response to nanomaterial exposure...
  24. pmc Detection of p53 mutations in precancerous gastric tissue
    C Morgan
    Human Molecular Pathology Group, Swansea Clinical School, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK
    Br J Cancer 89:1314-9. 2003
    ..pylori-positive and -negative groups. However, a small subpopulation of the H. pylori-negative patients had much higher levels of free radicals. This suggests a more prominent role for other factors in ROS production...
  25. doi request reprint Reflux composition influences the level of NF-κB activation and upstream kinase preference in oesophageal adenocarcinoma cells
    E McAdam
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea, SA2 8PP, United Kingdom
    Int J Cancer 136:527-35. 2015
    ..We identified further kinases important in acid or bile induced NF-κB signalling in oesophageal cells, which may provide suitable targets for therapeutic intervention. ..
  26. ncbi request reprint Do dose response thresholds exist for genotoxic alkylating agents?
    G J S Jenkins
    Swansea School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP and School of Biological Sciences, University of Wales, Swansea, UK
    Mutagenesis 20:389-98. 2005
    ..Finally, genotoxic thresholds are currently being described for acute exposures to single agents in vitro, however, dose response data for chronic exposures to complex mixtures are, as yet, a long way off...
  27. ncbi request reprint Identification of early p53 mutations in clam ileocystoplasties using restriction site mutation assay
    Kenneth D Ivil
    Department of Urology, University Hospital of Wales, Cardiff, United Kingdom
    Urology 70:905-9. 2007
    ....
  28. ncbi request reprint Mechanistic influences for mutation induction curves after exposure to DNA-reactive carcinogens
    Shareen H Doak
    School of Medicine, University of Wales Swansea, Singleton Park, Swansea, Wales, United Kingdom
    Cancer Res 67:3904-11. 2007
    ..A pragmatic threshold for carcinogenicity may therefore exist for such genotoxins...
  29. ncbi request reprint Differential expression of the MAD2, BUB1 and HSP27 genes in Barrett's oesophagus-their association with aneuploidy and neoplastic progression
    Shareen H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK
    Mutat Res 547:133-44. 2004
    ..HSP27 transcriptional patterns therefore present potential as a prognostic tool to predict the aggressiveness of oesophageal adenocarcinomas (OA)...
  30. doi request reprint Reprimo 824 G>C and p53R2 4696 C>G single nucleotide polymorphisms and colorectal cancer: a case-control disease association study
    William D Beasley
    Department of Colorectal Surgery, Singleton Hospital, Swansea NHS Trust Sketty, Swansea SA2 8QA Wales, UK
    Int J Colorectal Dis 23:375-81. 2008
    ..Single nucleotide polymorphisms (SNPs) of these genes have been discovered, but their effects on the genes' function and association with CRC is not known...