Derek W Hood

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. pmc Biosynthesis of cryptic lipopolysaccharide glycoforms in Haemophilus influenzae involves a mechanism similar to that required for O-antigen synthesis
    Derek W Hood
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom
    J Bacteriol 186:7429-39. 2004
  2. ncbi request reprint Three genes, lgtF, lic2C and lpsA, have a primary role in determining the pattern of oligosaccharide extension from the inner core of Haemophilus influenzae LPS
    Derek W Hood
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Microbiology 150:2089-97. 2004
  3. ncbi request reprint Novel lipopolysaccharide biosynthetic genes containing tetranucleotide repeats in Haemophilus influenzae, identification of a gene for adding O-acetyl groups
    Kate L Fox
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Mol Microbiol 58:207-16. 2005
  4. pmc lpt6, a gene required for addition of phosphoethanolamine to inner-core lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae
    J Claire Wright
    Molecular Infectious Diseases Group, Dept of Pediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom
    J Bacteriol 186:6970-82. 2004
  5. pmc Digalactoside expression in the lipopolysaccharide of Haemophilus influenzae and its role in intravascular survival
    Ruth Griffin
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, UK
    Infect Immun 73:7022-6. 2005
  6. pmc Identification of the functional initiation codons of a phase-variable gene of Haemophilus influenzae, lic2A, with the potential for differential expression
    Kevin Dixon
    University of Oxford, John Radcliffe Hospital, UK
    J Bacteriol 189:511-21. 2007
  7. ncbi request reprint Identification of a bifunctional lipopolysaccharide sialyltransferase in Haemophilus influenzae: incorporation of disialic acid
    Kate L Fox
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, United Kingdom
    J Biol Chem 281:40024-32. 2006
  8. pmc Duplicate copies of lic1 direct the addition of multiple phosphocholine residues in the lipopolysaccharide of Haemophilus influenzae
    Kate L Fox
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Infect Immun 76:588-600. 2008
  9. pmc Sialic acid mediated transcriptional modulation of a highly conserved sialometabolism gene cluster in Haemophilus influenzae and its effect on virulence
    Gaynor A Jenkins
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX39DS, UK
    BMC Microbiol 10:48. 2010
  10. doi request reprint Genes required for the synthesis of heptose-containing oligosaccharide outer core extensions in Haemophilus influenzae lipopolysaccharide
    Derek W Hood
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Microbiology 156:3421-31. 2010

Collaborators

Detail Information

Publications46

  1. pmc Biosynthesis of cryptic lipopolysaccharide glycoforms in Haemophilus influenzae involves a mechanism similar to that required for O-antigen synthesis
    Derek W Hood
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom
    J Bacteriol 186:7429-39. 2004
    ..influenzae genome, the hmg locus, is involved in the synthesis of glycoforms in which tetrasaccharide units are added en bloc, not stepwise, to the normal core glycoforms, similar to the biosynthesis of an O-antigen...
  2. ncbi request reprint Three genes, lgtF, lic2C and lpsA, have a primary role in determining the pattern of oligosaccharide extension from the inner core of Haemophilus influenzae LPS
    Derek W Hood
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Microbiology 150:2089-97. 2004
    ..Thus, lgtF, lic2C and lpsA make significant but very distinct contributions to the conservation and variable patterns of oligosaccharide extensions seen in H. influenzae LPS...
  3. ncbi request reprint Novel lipopolysaccharide biosynthetic genes containing tetranucleotide repeats in Haemophilus influenzae, identification of a gene for adding O-acetyl groups
    Kate L Fox
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Mol Microbiol 58:207-16. 2005
    ..These results provide genetic and structural evidence that H. influenzae oafA is required for phase variable O-acetylation of LPS and functional evidence to support the role of O-acetylation of LPS in pathogenesis...
  4. pmc lpt6, a gene required for addition of phosphoethanolamine to inner-core lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae
    J Claire Wright
    Molecular Infectious Diseases Group, Dept of Pediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom
    J Bacteriol 186:6970-82. 2004
    ..meningitidis and H. influenzae lpt6 genes have conserved residues across their lengths and are part of a family of proven or putative PEtn transferases present in a wide range of gram-negative bacteria...
  5. pmc Digalactoside expression in the lipopolysaccharide of Haemophilus influenzae and its role in intravascular survival
    Ruth Griffin
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, UK
    Infect Immun 73:7022-6. 2005
    ..Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo...
  6. pmc Identification of the functional initiation codons of a phase-variable gene of Haemophilus influenzae, lic2A, with the potential for differential expression
    Kevin Dixon
    University of Oxford, John Radcliffe Hospital, UK
    J Bacteriol 189:511-21. 2007
    ....
  7. ncbi request reprint Identification of a bifunctional lipopolysaccharide sialyltransferase in Haemophilus influenzae: incorporation of disialic acid
    Kate L Fox
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, United Kingdom
    J Biol Chem 281:40024-32. 2006
    ....
  8. pmc Duplicate copies of lic1 direct the addition of multiple phosphocholine residues in the lipopolysaccharide of Haemophilus influenzae
    Kate L Fox
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Infect Immun 76:588-600. 2008
    ..When bound, C-reactive protein leads to complement-mediated killing, indicating the potential biological significance of multiple PCho residues...
  9. pmc Sialic acid mediated transcriptional modulation of a highly conserved sialometabolism gene cluster in Haemophilus influenzae and its effect on virulence
    Gaynor A Jenkins
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX39DS, UK
    BMC Microbiol 10:48. 2010
    ..This study aimed to characterise the expression of genes required for the metabolism of sialic acid and to investigate the role of these genes in virulence...
  10. doi request reprint Genes required for the synthesis of heptose-containing oligosaccharide outer core extensions in Haemophilus influenzae lipopolysaccharide
    Derek W Hood
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Microbiology 156:3421-31. 2010
    ..Our studies confirm that losB1 and losB2 usually encode dd-heptosyl- and ld-heptosyl transferases, respectively, and that losA1 and losA2 encode glycosyltransferases that play a role in OS extensions of NTHi LPS...
  11. ncbi request reprint Specific amino acids of the glycosyltransferase LpsA direct the addition of glucose or galactose to the terminal inner core heptose of Haemophilus influenzae lipopolysaccharide via alternative linkages
    Mary E Deadman
    Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, United Kingdom
    J Biol Chem 281:29455-67. 2006
    ....
  12. ncbi request reprint The role of lex2 in lipopolysaccharide biosynthesis in Haemophilus influenzae strains RM7004 and RM153
    Ruth Griffin
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Microbiology 149:3165-75. 2003
    ..Investigation of the expression of the lex2 locus indicated that the genes are co-transcribed and that both reading frames are required for addition of this second beta-glucose in a phase-variable manner...
  13. ncbi request reprint Involvement of genes of genome maintenance in the regulation of phase variation frequencies in Neisseria meningitidis
    Patricia Martin
    Molecular Infectious Diseases Group, University of Oxford, Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, UK
    Microbiology 150:3001-12. 2004
    ..dam(+)/drg and dam/drg(+), did not modify phase variation frequencies. In contrast to the effects of certain genes on homopolymeric tracts, none of the genetic factors investigated affected the stability of tetranucleotide repeat tracts...
  14. doi request reprint RecJ, ExoI and RecG are required for genome maintenance but not for generation of genetic diversity by repeat-mediated phase variation in Haemophilus influenzae
    Gaurav A Kumar
    Molecular Infectious Diseases Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Mutat Res 640:46-53. 2008
    ..We conclude that RecJ, ExoI and RecG are required for maintenance of the H. influenzae genome but none of these enzymes influence the generation of genetic diversity through mutations in the tetranucleotide repeat tracts of this species...
  15. pmc Microsatellite instability regulates transcription factor binding and gene expression
    Patricia Martin
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Proc Natl Acad Sci U S A 102:3800-4. 2005
    ..Thus, phase-variable transcription of nadA potentially incorporates interplay between stochastic (mutational) and prescriptive (classical) mechanisms of gene regulation...
  16. pmc High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae
    Alison J Cody
    Molecular Infectious Diseases Group, Department of Paediatrics, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK
    Infect Genet Evol 3:57-66. 2003
    ..These findings provide a population based foundation for a better understanding of the role of NTHi LPS as a virulence factor and its potential as a candidate vaccine...
  17. ncbi request reprint Identification of a gene (lpt-3) required for the addition of phosphoethanolamine to the lipopolysaccharide inner core of Neisseria meningitidis and its role in mediating susceptibility to bactericidal killing and opsonophagocytosis
    Fiona G Mackinnon
    Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, John Radcliffe Hospital, Oxford OX3 9DS, UK
    Mol Microbiol 43:931-43. 2002
    ....
  18. pmc Investigations into genome diversity of Haemophilus influenzae using whole genome sequencing of clinical isolates and laboratory transformants
    Peter M Power
    University of Oxford Department of Paediatrics, Medical Sciences Division, John Radcliffe Hospital, Headington, Oxford, UK
    BMC Microbiol 12:273. 2012
    ..Haemophilus influenzae is an important human commensal pathogen associated with significant levels of disease. High-throughput DNA sequencing was used to investigate differences in genome content within this species...
  19. doi request reprint Haemophilus influenzae biofilms: hypothesis or fact?
    E Richard Moxon
    University of Oxford Department of Paediatrics, Oxford OX3 9DS, UK
    Trends Microbiol 16:95-100. 2008
    ..Uncritical acceptance of a distinctive NTHi biofilm phenotype has the potential to mislead and could confuse and compromise research efforts aimed at improving management and prevention of NTHi diseases of the human respiratory tract...
  20. pmc Novel type IV secretion system involved in propagation of genomic islands
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Headington, OX3 9DU Oxford, United Kingdom
    J Bacteriol 189:761-71. 2007
    ..Moreover, the expression of this T4SS was found to be positively regulated by one of its components, the tfc24 gene. We concluded that this gene cluster represents a novel family of T4SSs involved in propagation of genomic islands...
  21. pmc Sequence and functional analyses of Haemophilus spp. genomic islands
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, NDCLS, University of Oxford, Headley Way, Oxford OX3 9DU, UK
    Genome Biol 8:R237. 2007
    ..More clarity comes from this comparative analysis of seven complete sequences of the ICEHin1056 genomic island subfamily...
  22. pmc Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, UK
    Cell Microbiol 10:2377-86. 2008
    ..The most recently described GI type T4SSs play a key role in the horizontal transfer of a wide variety of genomic islands derived from a broad spectrum of bacterial strains...
  23. pmc Genomic islands: tools of bacterial horizontal gene transfer and evolution
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford, UK
    FEMS Microbiol Rev 33:376-93. 2009
    ..Depending on the composition of gene modules, the same type of GEIs can promote survival of pathogenic as well as environmental bacteria...
  24. pmc Recombinational switching of the Clostridium difficile S-layer and a novel glycosylation gene cluster revealed by large-scale whole-genome sequencing
    Kate E Dingle
    Nuffield Department of Clinical Medicine, Oxford Biomedical Research Centre, John Radcliffe Hospital, UK
    J Infect Dis 207:675-86. 2013
    ..Our purpose was to understand the diversity and evolution of slpA and nearby genes also encoding immunodominant cell surface antigens...
  25. pmc Transferable antibiotic resistance elements in Haemophilus influenzae share a common evolutionary origin with a diverse family of syntenic genomic islands
    Zaini Mohd-Zain
    Infectious Diseases and Clinical Microbiology, John Radcliffe Hospital, University of Oxford, Headington, Oxford, OX3 9DU, UK
    J Bacteriol 186:8114-22. 2004
    ..strain B13. This is the first report of a diverse family of related syntenic genomic islands with a deep evolutionary origin, and our findings challenge the view that genomic islands consist only of independently evolving modules...
  26. ncbi request reprint The genome sequence of Haemophilus influenzae
    Derek W Hood
    Molecular Infectious Diseases Group, Department of Pediatrics, University of Oxford, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Methods Mol Med 71:147-59. 2003
  27. pmc Application of capillary electrophoresis mass spectrometry and liquid chromatography multiple-step tandem electrospray mass spectrometry to profile glycoform expression during Haemophilus influenzae pathogenesis in the chinchilla model of experimental oti
    Susanna L Lundström
    Karolinska Institutet and University College of South Stockholm, Clinical Research Center, Novum, S 141 86 Huddinge, Sweden
    Infect Immun 76:3255-67. 2008
    ..As previously observed, sialic acid-containing glycoforms were detected during the early stages of infection, but a trend toward more-truncated and less-complex LPS glycoforms that lacked sialic acid was found as disease progressed...
  28. doi request reprint Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain R2846
    Susanna L Lundström
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Novum, S 14186 Huddinge, Sweden
    Biochemistry 47:6025-38. 2008
    ..The LPS from strain R2846 losB1/ losB2 expressed no noncore heptose, consistent with losB2 directing the addition of ld-heptose...
  29. pmc Neisseria meningitidis escape from the bactericidal activity of a monoclonal antibody is mediated by phase variation of lgtG and enhanced by a mutator phenotype
    Christopher D Bayliss
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Infect Immun 76:5038-48. 2008
    ....
  30. pmc Phosphorylation of the lipid A region of meningococcal lipopolysaccharide: identification of a family of transferases that add phosphoethanolamine to lipopolysaccharide
    Andrew D Cox
    Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada
    J Bacteriol 185:3270-7. 2003
    ..We propose that NMB1638 encodes a phosphoethanolamine transferase specific for lipid A and propose naming the gene "lptA," for "LPS phosphoethenolamine transferase for lipid A."..
  31. ncbi request reprint Novel globoside-like oligosaccharide expression patterns in nontypeable Haemophilus influenzae lipopolysaccharide
    Susanna L Lundström
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Huddinge, Sweden
    FEBS J 274:4886-903. 2007
    ..Nontypeable H. influenzae strain 1200 expressed identical LPS structures to 1268 with the difference that strain 1200 LPS had acetates substituting HepIII, whereas strain 1268 LPS has glycine at the same position...
  32. ncbi request reprint Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence
    Elke K H Schweda
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Novum, S 141 86 Huddinge, Sweden
    Int J Med Microbiol 297:297-306. 2007
    ..Here, the structure, genetics and expression of LPS glycoforms in the outer core are reviewed as well as their implication on virulence...
  33. ncbi request reprint Identification and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus influenzae
    Andrew D Cox
    Institute for Biological Sciences, National Research Council, Ottawa, Canada
    Eur J Biochem 269:4009-19. 2002
    ....
  34. ncbi request reprint Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus influenzae. Identification of L-glycerol-D-manno-heptose in the outer-core region in three clinical isolates
    Martin Månsson
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Huddinge, Sweden
    Eur J Biochem 270:610-24. 2003
    ....
  35. ncbi request reprint Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae
    Martin Månsson
    Clinical Research Centre, Karolinska Institutet, Huddinge, Sweden
    Eur J Biochem 270:2979-91. 2003
    ..This is the first time a branching sugar residue has been reported in the outer-core region of H. influenzae LPS. Additionally, a PEtn group was identified at O-3 of the distal heptose residue in the inner-core...
  36. pmc Host-derived sialic acid is incorporated into Haemophilus influenzae lipopolysaccharide and is a major virulence factor in experimental otitis media
    Valerie Bouchet
    Maxwell Finland Laboratory for Infectious Diseases, Division of Pediatric Infectious Diseases, Boston University School of Medicine, Boston Medical Center, 774 Albany Street, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 100:8898-903. 2003
    ..We conclude that sialylated LPS glycoforms play a key role in pathogenicity of nontypeable H. influenzae and depend on scavenging the essential precursors from the host during the infection...
  37. ncbi request reprint Structural analysis of lipopolysaccharides from Haemophilus influenzae serotype f. Structural diversity observed in three strains
    Håkan H Yildirim
    Clinical Research Centre, Karolinska Institutet, Sweden
    Eur J Biochem 270:3153-67. 2003
    ..ESI-MSn on dephosphorylated and permethylated OS provided information on the existence of additional minor isomeric glycoforms...
  38. ncbi request reprint Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003
    Martin Månsson
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Huddinge, Sweden
    Eur J Biochem 269:808-18. 2002
    ..Disialylated species were also detected. In addition, a minor substitution of ester-linked glycine at HepIII and Kdo was observed...
  39. ncbi request reprint Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus influenzae
    Malin K Landerholm
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Novum, Huddinge, Sweden
    Eur J Biochem 271:941-53. 2004
    ..Major acetylation sites were identified at O-4 of Gal and O-3 of HepIII. Additionally, both strains express glycine, and strain 11 also expresses detectable amounts of N-acetylneuraminic acid...
  40. ncbi request reprint An alternate pattern for globoside oligosaccharide expression in Haemophilus influenzae lipopolysaccharide: structural diversity in nontypeable strain 1124
    Håkan H Yildirim
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Novum, S 141 86 Huddinge, Sweden
    Biochemistry 44:5207-24. 2005
    ..influenzae that has not been reported previously. In addition, the LPS was found to carry phosphorylcholine, O-linked glycine, and a third PEA group which was linked to O3 of HepIII...
  41. ncbi request reprint Structural investigation of lipopolysaccharides from nontypeable Haemophilus influenzae: investigation of inner-core phosphoethanolamine addition in NTHi strain 981
    Ann Sofie Tinnert
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Novum, S 141 86 Huddinge, Sweden
    Carbohydr Res 340:1900-7. 2005
    ..However, it is not responsible for adding PEtn to O-3 of HepIII since the 981lpt6 mutant still had full substitution with PEtn at HepIII...
  42. ncbi request reprint Complex O-acetylation in non-typeable Haemophilus influenzae lipopolysaccharide: evidence for a novel site of O-acetylation
    Håkan H Yildirim
    Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, Novum, S 141 86 Huddinge, Sweden
    Carbohydr Res 340:2598-611. 2005
    ..This is the first study to show that an acetyl group can substitute HepI of the inner-core region of H. influenzae LPS...
  43. pmc Role of complement in defense of the middle ear revealed by restoring the virulence of nontypeable Haemophilus influenzae siaB mutants
    Marisol A Figueira
    Section of Pediatric Infectious Diseases, Maxwell Finland Laboratory for Infectious Diseases, Room 508, 774 Albany Street, Boston Medical Center, Boston, MA 02118, USA
    Infect Immun 75:325-33. 2007
    ..influenzae strains, which in turn may impact their virulence. These data demonstrate a central role for complement in innate immune defenses against NT H. influenzae infections and specifically EOM...
  44. pmc Elucidation of the monoclonal antibody 5G8-reactive, virulence-associated lipopolysaccharide epitope of Haemophilus influenzae and its role in bacterial resistance to complement-mediated killing
    Ruth Griffin
    Centre for Molecular Microbiology and Infection, Level 3, Flowers Building, Imperial College, London SW7 2AZ, United Kingdom
    Infect Immun 73:2213-21. 2005
    ..influenzae LPS, and we show that the ability to synthesize this novel tetrasaccharide was associated with enhanced bacterial resistance to complement-mediated killing...
  45. ncbi request reprint Electrophoretic and mass spectrometric strategies for profiling bacterial lipopolysaccharides
    Jianjun Li
    Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6
    Mol Biosyst 1:46-52. 2005
    ..influenzae strain 375 (375.1) were studied using on-line CE-MS/MS. With the separation provided by CE, two isomeric glycoforms differing in the location of phosphoethanolamine substituents were characterized by tandem mass spectrometry...
  46. ncbi request reprint Structural characterization of sialylated glycoforms of H. influenzae by electrospray mass spectrometry: fragmentation of protonated and sodiated O-deacylated lipopolysaccharides
    Jianjun Li
    Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada, K1A 0R6
    Rapid Commun Mass Spectrom 21:952-60. 2007
    ..This methodology is of general applicability for determination of common sialylated oligosaccharide extension in bacterial LPS...