J Green

Summary

Affiliation: University of Sheffield
Country: UK

Publications

  1. pmc Cytochrome bd-I in Escherichia coli is less sensitive than cytochromes bd-II or bo'' to inhibition by the carbon monoxide-releasing molecule, CORM-3: N-acetylcysteine reduces CO-RM uptake and inhibition of respiration
    Helen E Jesse
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, S10 2TN, UK
    Biochim Biophys Acta 1834:1693-703. 2013
  2. pmc Cyclic-AMP and bacterial cyclic-AMP receptor proteins revisited: adaptation for different ecological niches
    Jeffrey Green
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK Electronic address
    Curr Opin Microbiol 18:1-7. 2014
  3. pmc Alternate SlyA and H-NS nucleoprotein complexes control hlyE expression in Escherichia coli K-12
    James K Lithgow
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 66:685-98. 2007
  4. pmc Mycobacterium tuberculosis cAMP receptor protein (Rv3676) differs from the Escherichia coli paradigm in its cAMP binding and DNA binding properties and transcription activation properties
    Melanie Stapleton
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 285:7016-27. 2010
  5. pmc Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations
    Matthew D Rolfe
    Department of Molecular Biology and Biotechnology, The Krebs Institute, University of Sheffield, Sheffield S10 2TN, UK
    Open Biol 2:120091. 2012
  6. pmc Reprogramming of Escherichia coli K-12 metabolism during the initial phase of transition from an anaerobic to a micro-aerobic environment
    Eleanor W Trotter
    Department of Molecular Biology and Biotechnology, The Krebs Institute, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 6:e25501. 2011
  7. ncbi request reprint Identification of a surface of FNR overlapping activating region 1 that is required for repression of gene expression
    J Green
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 274:10244-8. 1999
  8. ncbi request reprint Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:2865-75. 1997
  9. ncbi request reprint Bacterial redox sensors
    Jeffrey Green
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Nat Rev Microbiol 2:954-66. 2004
  10. doi request reprint Bacterial sensors of oxygen
    Jeffrey Green
    University of Sheffield, UK
    Curr Opin Microbiol 12:145-51. 2009

Collaborators

Detail Information

Publications76

  1. pmc Cytochrome bd-I in Escherichia coli is less sensitive than cytochromes bd-II or bo'' to inhibition by the carbon monoxide-releasing molecule, CORM-3: N-acetylcysteine reduces CO-RM uptake and inhibition of respiration
    Helen E Jesse
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, S10 2TN, UK
    Biochim Biophys Acta 1834:1693-703. 2013
    ....
  2. pmc Cyclic-AMP and bacterial cyclic-AMP receptor proteins revisited: adaptation for different ecological niches
    Jeffrey Green
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK Electronic address
    Curr Opin Microbiol 18:1-7. 2014
    ..It appears that the cyclic-AMP-CRP regulatory system has been adapted to respond to distinct external and internal inputs across a broad sensitivity range that is, at least in part, determined by bacterial lifestyles. ..
  3. pmc Alternate SlyA and H-NS nucleoprotein complexes control hlyE expression in Escherichia coli K-12
    James K Lithgow
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 66:685-98. 2007
    ..When H-NS is dominant RNAP binding is inhibited and hlyE expression is silenced; when SlyA is dominant H-NS binding is inhibited allowing RNAP access to the promoter facilitating hlyE transcription...
  4. pmc Mycobacterium tuberculosis cAMP receptor protein (Rv3676) differs from the Escherichia coli paradigm in its cAMP binding and DNA binding properties and transcription activation properties
    Melanie Stapleton
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 285:7016-27. 2010
    ..Thus, M. tuberculosis CRP has evolved several distinct characteristics, compared with the Escherichia coli CRP paradigm, to allow it to regulate gene expression against a background of high concentrations of cAMP...
  5. pmc Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations
    Matthew D Rolfe
    Department of Molecular Biology and Biotechnology, The Krebs Institute, University of Sheffield, Sheffield S10 2TN, UK
    Open Biol 2:120091. 2012
    ..Thus, this approach illustrates how systematic examination of regulatory responses in stable and dynamic environments yields new mechanistic insights into adaptive processes...
  6. pmc Reprogramming of Escherichia coli K-12 metabolism during the initial phase of transition from an anaerobic to a micro-aerobic environment
    Eleanor W Trotter
    Department of Molecular Biology and Biotechnology, The Krebs Institute, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 6:e25501. 2011
    ..However, the dynamics of adaptation when bacteria experience changes in O₂ availability are understudied. The model bacterium and facultative anaerobe Escherichia coli K-12 provides an ideal system for exploring this process...
  7. ncbi request reprint Identification of a surface of FNR overlapping activating region 1 that is required for repression of gene expression
    J Green
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 274:10244-8. 1999
    ..Thus, FNR-mediated repression at promoters with multiple FNR sites requires regions of FNR that are different from, but overlap, AR1...
  8. ncbi request reprint Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:2865-75. 1997
    ..Studies with the FNR-activated and amino-acid-inducible asparaginase II gene (ansB) showed that IHF and a component of the Arr-containing fraction (but not HU) interact with the corresponding ansB promoter...
  9. ncbi request reprint Bacterial redox sensors
    Jeffrey Green
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Nat Rev Microbiol 2:954-66. 2004
    ..Advances in biochemical analyses are revealing a range of elegant solutions that have evolved to allow bacteria to sense different redox signals...
  10. doi request reprint Bacterial sensors of oxygen
    Jeffrey Green
    University of Sheffield, UK
    Curr Opin Microbiol 12:145-51. 2009
    ..When utilizing heme as the sensor, the capacity of O(2) to form a reversible Fe-O(2) bond or alternatively the oxidation of the heme iron atom itself is used to detect O(2) and switch regulators between active and inactive forms...
  11. ncbi request reprint Downregulation of Escherichia coli yfiD expression by FNR occupying a site at -93.5 involves the AR1-containing face of FNR
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 29:1113-23. 1998
    ..The FNR variant F92-->S was notable because, although it activated transcription of yfiD (two FNR sites), it was unable to activate transcription from model Class I and II promoters, which contain only a single FNR site...
  12. ncbi request reprint FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites
    W Meng
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, UK
    Microbiology 143:1521-32. 1997
    ....
  13. ncbi request reprint Properties of FNR proteins substituted at each of the five cysteine residues
    J Green
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 8:61-8. 1993
    ....
  14. ncbi request reprint A novel promoter architecture for microaerobic activation by the anaerobic transcription factor FNR
    F A Marshall
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    Mol Microbiol 39:747-53. 2001
    ..More or less oxygen reduced expression dramatically. This pattern of response was dependent on the combination of a high-affinity site at the activating class II position and a lower affinity site at the upstream position...
  15. ncbi request reprint HlyX, the FNR homologue of Actinobacillus pleuropneumoniae, is a [4Fe-4S]-containing oxygen-responsive transcription regulator that anaerobically activates FNR-dependent class I promoters via an enhanced AR1 contact
    J Green
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 24:593-605. 1997
    ..The AR1 of HlyX is partially characterized by a surface-exposed region around amino acid A187, which confers the altered specificity and provides an explanation for the existence of distinct but overlapping HlyX and FNR regulons...
  16. ncbi request reprint The molecular basis for the differential regulation of the hlyE-encoded haemolysin of Escherichia coli by FNR and HlyX lies in the improved activating region 1 contact of HlyX
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:3785-93. 1997
    ..The FNR variant, FNR-A225T, combined the properties of FNR (good activation from Class II promoters) and HlyX (good activation of Class I promoters) and conferred the haemolytic phenotype...
  17. ncbi request reprint Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLP
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Adv Microb Physiol 44:1-34. 2001
    ..And, at the level of RNA-polymerase-interaction, different combinations of three discrete regions contacting the polymerase (the activating regions) are used for polymerase recruitment and promoting transcription...
  18. ncbi request reprint Anaerobic acquisition of [4FE 4S] clusters by the inactive FNR(C20S) variant and restoration of activity by second-site amino acid substitutions
    E T Ralph
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 39:1199-211. 2001
    ....
  19. ncbi request reprint Regulation of transcription at the ndh promoter of Escherichia coli by FNR and novel factors
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 12:433-44. 1994
    ..These putative amino acid-responsive activators may thus offset FNR-mediated repression and maintain a low level of anaerobic ndh expression for regulating the NAD+/NADH ratio during growth in rich media...
  20. ncbi request reprint Two operons that encode FNR-like proteins in Lactococcus lactis
    D O Gostick
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 31:1523-35. 1999
    ..It was concluded tentatively that FlpA and FlpB regulate overlapping modulons, including systems concerned with zinc uptake, in response to metal ion or oxidative stress...
  21. ncbi request reprint The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis
    J Green
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 20:1043-55. 1996
    ....
  22. ncbi request reprint A role for iron in transcriptional activation by FNR
    J Green
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, UK
    FEBS Lett 329:55-8. 1993
    ..Since open complex formation is an essential pre-requisite for transcription, it is concluded that transcriptional activation by FNR is mediated by a ferrous iron cofactor...
  23. pmc Altering the anaerobic transcription factor FNR confers a hemolytic phenotype on Escherichia coli K12
    E T Ralph
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 95:10449-52. 1998
    ..It is concluded that altering a resident transcription regulator, or acquisition of a competent heterologous regulator, could generate a pool of hemolytic, and therefore more virulent, strains of E. coli in nature...
  24. ncbi request reprint YeiL, the third member of the CRP-FNR family in Escherichia coli
    M F Anjum
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 146:3157-70. 2000
    ....
  25. ncbi request reprint In vivo and in vitro mutants of FNR the anaerobic transcriptional regulator of E. coli
    A D Sharrocks
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 270:119-22. 1990
    ..Four independent in vivo mutants contained identical Gly-96----Asp substitutions, which may inactivate FNR by distorting a sharp turn between beta-strands in the predicted structure...
  26. ncbi request reprint FNR activates and represses transcription in vitro
    A D Sharrocks
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, U K
    Proc Biol Sci 245:219-26. 1991
    ....
  27. ncbi request reprint Co-regulation of lipoamide dehydrogenase and 2-oxoglutarate dehydrogenase synthesis in Escherichia coli: characterisation of an ArcA binding site in the lpd promoter
    L Cunningham
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEMS Microbiol Lett 169:403-8. 1998
    ..PdhR was not bound to the lpd promoter nor was ArcA bound specifically to the pdh promoter. These results support the view that co-regulation of the lpd and sdh promoters is mediated primarily by ArcA...
  28. ncbi request reprint Characterization of the FNR protein of Escherichia coli, an iron-binding transcriptional regulator
    J Green
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, U K
    Proc Biol Sci 244:137-44. 1991
    ..A role for iron in FNR-mediated gene expression was confirmed by using cultures in which FNR was inactivated by growth in the presence of the specific chelator, ferrozine, but protected by ferrous iron...
  29. ncbi request reprint Structure-function relationships of a novel bacterial toxin, hemolysin E. The role of alpha G
    A Atkins
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 275:41150-5. 2000
    ..Thus once associated with a membrane, immobilizing alpha(G) inhibits HlyE activity at a late stage of pore formation, whereas in solution it prevents aggregation and consequent inactivation...
  30. ncbi request reprint E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy
    A J Wallace
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, United Kingdom
    Cell 100:265-76. 2000
    ..Taken together, the data from these two structural techniques allow us to propose a simple model for the structure of the pore and for membrane interaction...
  31. doi request reprint Bacterial delivery of a novel cytolysin to hypoxic areas of solid tumors
    R M Ryan
    Tumor Targeting Group, Academic Unit of Pathology, University of Sheffield Medical School, Sheffield, UK
    Gene Ther 16:329-39. 2009
    ..Our system represents a promising new strategy for delivering gene therapy to poorly vascularized regions of tumors and shows, for the first time, the efficacy of HlyE as an anti-tumor agent...
  32. pmc NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, Hmp
    Hugo Cruz-Ramos
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK
    EMBO J 21:3235-44. 2002
    ..Thus, FNR can respond to not only O(2), but also NO, with major implications for global gene regulation in bacteria. We propose an NO-mediated mechanism of hmp regulation by which E.coli responds to NO challenge...
  33. pmc Purification and properties of the lipoate protein ligase of Escherichia coli
    D E Green
    Krebs Institute for Biomolecular Research, Department of Molecular Biology, University of Sheffield, U K
    Biochem J 309:853-62. 1995
    ..Studies with lplA mutants lacking LPL activity indicated that E. coli possesses another distinct lipoylation system, although no such activity could be detected in vitro...
  34. ncbi request reprint Transcriptional responses of Escherichia coli to S-nitrosoglutathione under defined chemostat conditions reveal major changes in methionine biosynthesis
    Janet Flatley
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Biol Chem 280:10065-72. 2005
    ..The data presented here reveal new genes important for nitrosative stress tolerance and demonstrate that methionine biosynthesis is a casualty of nitrosative stress...
  35. ncbi request reprint In vivo cycling of the Escherichia coli transcription factor FNR between active and inactive states
    David P Dibden
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 151:4063-70. 2005
    ..Thus, the simplest explanation for the observations reported here is that the FNR protein can be switched between inactive and active forms in vivo in the absence of de novo protein synthesis...
  36. ncbi request reprint Escherichia coli transcriptome dynamics during the transition from anaerobic to aerobic conditions
    Jonathan D Partridge
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 281:27806-15. 2006
    ....
  37. pmc In vivo demonstration of FNR dimers in response to lower O(2) availability
    Adrian J Jervis
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Bacteriol 189:2930-2. 2007
    ..Here, two-hybrid assays show that iron-sulfur cluster-dependent FNR dimers are formed in vivo in response to lower O(2) availability, consistent with the current model of FNR activation...
  38. ncbi request reprint The Escherichia coli yhjA gene, encoding a predicted cytochrome c peroxidase, is regulated by FNR and OxyR
    Jonathan D Partridge
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK
    Microbiology 153:1499-507. 2007
    ....
  39. pmc The O2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion
    Adrian J Jervis
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 106:4659-64. 2009
    ..These data lead to a simple model of the FNR switch with physiological implications for the ability of FNR proteins to operate over different ranges of in vivo O(2) concentrations...
  40. doi request reprint Escherichia coli K-12 YfgF is an anaerobic cyclic di-GMP phosphodiesterase with roles in cell surface remodelling and the oxidative stress response
    Melissa M Lacey
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    Microbiology 156:2873-86. 2010
    ..coli K-12 and in the response to peroxide shock, with implications for integrating three global regulatory networks, i.e. oxygen regulation, cyclic di-GMP signalling and the oxidative stress response...
  41. ncbi request reprint Miscoordination of the iron-sulfur clusters of the anaerobic transcription factor, FNR, allows simple repression but not activation
    Colin Scott
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 277:1749-54. 2002
    ....
  42. pmc Regulation of Escherichia coli hemolysin E expression by H-NS and Salmonella SlyA
    Neil R Wyborn
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Bacteriol 186:1620-8. 2004
    ..Finally, the identification of a SlyA binding site that overlaps the H-NS I site in PhlyE suggests a mechanism to explain how SlyA overproduction enhances hlyE expression by antagonizing the negative effects of H-NS...
  43. ncbi request reprint FNR-mediated regulation of hyp expression in Escherichia coli
    Sarah L Messenger
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    FEMS Microbiol Lett 228:81-6. 2003
    ..In vitro gel retardation analysis suggested that the primary interaction occurs at the downstream FNR half-site. Possible explanations for these observations and the implications for other FNR-regulated promoters are discussed...
  44. ncbi request reprint Escherichia coli aconitases and oxidative stress: post-transcriptional regulation of sodA expression
    Yue Tang
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 148:1027-37. 2002
    ..coli serve as a protective buffer against the basal level of oxidative stress that accompanies aerobic growth by acting as a sink for reactive oxygen species and by modulating translation of the sodA transcript...
  45. ncbi request reprint Structure of the hemolysin E (HlyE, ClyA, and SheA) channel in its membrane-bound form
    Svetomir B Tzokov
    Department of Molecular Biology and Biotechnology, Krebs Institute for Biomolecular Research, The University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 281:23042-9. 2006
    ..The reconstructed pores were significantly longer than the maximum dimension of the water-soluble molecule, indicating that conformational changes occur on pore formation...
  46. ncbi request reprint Transition of Escherichia coli from aerobic to micro-aerobic conditions involves fast and slow reacting regulatory components
    Jonathan D Partridge
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 282:11230-7. 2007
    ....
  47. pmc Transcription activation by FNR: evidence for a functional activating region 2
    Timo Blake
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    J Bacteriol 184:5855-61. 2002
    ..Thus, it was suggested that Lys49 and Lys50 form part of a functional AR2 in FNR...
  48. pmc Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation
    Steven T Pullan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    J Bacteriol 189:1845-55. 2007
    ....
  49. ncbi request reprint Expression of the Escherichia coli yfiD gene responds to intracellular pH and reduces the accumulation of acidic metabolic end products
    Neil R Wyborn
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK
    Microbiology 148:1015-26. 2002
    ....
  50. ncbi request reprint DNA target sequence and FNR-dependent gene expression
    Colin Scott
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, UK
    FEBS Lett 541:97-101. 2003
    ..Changing the NCT sequence did not affect basal activity but altered anaerobic induction by as much as an order of magnitude. Thus, the NCT sequence is a fundamental component in setting the dynamic range of the FNR switch...
  51. doi request reprint Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP
    Jonathan D Partridge
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, UK
    Microbiology 154:608-18. 2008
    ..Thus, although the region of the ydhY-T promoter containing the -16 and +15 heptamers was recognized by NarL in vitro, mutation of these heptamers did not affect NarL-mediated repression in vivo...
  52. ncbi request reprint Switching aconitase B between catalytic and regulatory modes involves iron-dependent dimer formation
    Yue Tang
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 56:1149-58. 2005
    ..Furthermore, the relative effects of iron on these processes provide a simple iron-mediated dimerization mechanism for switching the AcnB protein between catalytic and regulatory roles...
  53. ncbi request reprint Post-transcriptional regulation of bacterial motility by aconitase proteins
    Yue Tang
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    Mol Microbiol 51:1817-26. 2004
    ..The lower levels of FtsH protease activity then influence sigma32, DnaK and, ultimately, FliC production...
  54. doi request reprint The formation and structure of Escherichia coli K-12 haemolysin E pores
    Stuart Hunt
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, UK
    Microbiology 154:633-42. 2008
    ....
  55. ncbi request reprint Properties of haemolysin E (HlyE) from a pathogenic Escherichia coli avian isolate and studies of HlyE export
    Neil R Wyborn
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 150:1495-505. 2004
    ..Finally, disruption of genes associated with cell envelope function, including tatC, impairs HlyE export, indicating that outer membrane integrity is important for effective HlyE secretion...
  56. ncbi request reprint Hemolysin E (HlyE, ClyA, SheA) and related toxins
    Stuart Hunt
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK
    Adv Exp Med Biol 677:116-26. 2010
    ..We review the current knowledge of HlyE structure and function in its solution and pore forms, models for membrane insertion, its potential use in biotechnology applications and its relationship to a wider superfamily of toxins...
  57. ncbi request reprint Interaction of the Salmonella typhimurium transcription and virulence factor SlyA with target DNA and identification of members of the SlyA regulon
    Melanie R Stapleton
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 277:17630-7. 2002
    ..typhimurium genome annotation is complete...
  58. ncbi request reprint AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates
    Lindsay Blank
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 148:133-46. 2002
    ....
  59. pmc PhoP-responsive expression of the Salmonella enterica serovar typhimurium slyA gene
    Valia A Norte
    Department of Molecular Biology and Biotechnology, Krebs Institute for Biomolecular Research, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Bacteriol 185:3508-14. 2003
    ..We present here evidence for a direct link between two transcription factors (PhoP and SlyA) crucial for Salmonella virulence...
  60. ncbi request reprint DNA recognition by the Salmonella enterica serovar Typhimurium transcription factor SlyA
    Fouzia Haider
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Int Microbiol 11:245-50. 2008
    ..Combining the structure-function studies described here and elsewhere with the structures of MarR family proteins suggests a possible model for regulation of SlyA binding to DNA...
  61. ncbi request reprint The crystal structures of Lactococcus lactis MG1363 Dps proteins reveal the presence of an N-terminal helix that is required for DNA binding
    Timothy J Stillman
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, Firth Court, Western Bank, University of Sheffield, Sheffield S10 2TN, UK
    Mol Microbiol 57:1101-12. 2005
    ..coli, Bacillus brevis and Mycobacterium smegmatis Dps:DNA complexes, in which DNA interacts with crystalline Dps phases, L. lactis DNA:Dps complexes appeared as non-crystalline aggregates of protein and DNA in electron micrographs...
  62. ncbi request reprint Use of bacteria in anti-cancer therapies
    Rachel M Ryan
    Tumour Targeting Group, Division of Genomic Medicine, University of Sheffield, UK
    Bioessays 28:84-94. 2006
    ..Here, we review the evidence for the success of these in pre-clinical models and clinical trials, as single modality treatments and in combination with conventional cancer therapies...
  63. ncbi request reprint SlyA and H-NS regulate transcription of the Escherichia coli K5 capsule gene cluster, and expression of slyA in Escherichia coli is temperature-dependent, positively autoregulated, and independent of H-NS
    David Corbett
    Faculty of Life Sciences, University of Manchester, Smith Bldg, Oxford Rd, Manchester M13 9PT, United Kingdom
    J Biol Chem 282:33326-35. 2007
    ..In addition, we show that expression of the E. coli slyA gene is temperature-regulated, positively autoregulated, and independent of H-NS...
  64. pmc A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor
    Lisa Rickman
    Division of Mycobacterial Research, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Mol Microbiol 56:1274-86. 2005
    ..In summary, it has been shown that Rv3676 is a direct regulator of rpfA expression, and because rpfA codes for a resuscitation promoting factor this may implicate Rv3676 in reactivation of dormant M. tuberculosis infections...
  65. ncbi request reprint Regulation of ndh expression in Escherichia coli by Fis
    Laura Jackson
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 150:407-13. 2004
    ....
  66. ncbi request reprint E. coli aconitase B structure reveals a HEAT-like domain with implications for protein-protein recognition
    Colin H Williams
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Nat Struct Biol 9:447-52. 2002
    ..This domain packs against the remainder of the protein to form a tunnel leading to the aconitase active site, potentially for substrate channeling...
  67. pmc Transcription regulation by tandem-bound FNR at Escherichia coli promoters
    Anne M L Barnard
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Bacteriol 185:5993-6004. 2003
    ..5 and a second upstream DNA site for FNR, was also constructed. Again, promoter activity was repressed by FNR when the upstream-bound FNR was located at particular positions...
  68. ncbi request reprint Detection of sulfide release from the oxygen-sensing [4Fe-4S] cluster of FNR
    Jason C Crack
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 281:18909-13. 2006
    ..This demonstrates that the reaction between O2 and the [4Fe-4S]2+ cluster does not require sulfide oxidation and hence must entail iron oxidation...
  69. ncbi request reprint Location of the Escherichia coli RNA polymerase alpha subunit C-terminal domain at an FNR-dependent promoter: analysis using an artificial nuclease
    Anne M L Barnard
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    FEBS Lett 558:13-8. 2004
    ..coli transcription activator that is related to FNR. In complementary experiments, we show that the second alpha subunit C-terminal domain of RNA polymerase can be repositioned by upstream-bound CRP, but not by upstream-bound FNR...
  70. doi request reprint Reactions of nitric oxide and oxygen with the regulator of fumarate and nitrate reduction, a global transcriptional regulator, during anaerobic growth of Escherichia coli
    Jason C Crack
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich, United Kingdom
    Methods Enzymol 437:191-209. 2008
    ..This chapter describes some of the methods used to produce active [4Fe-4S] FNR protein and investigates the reaction of the [4Fe-4S](2+) cluster with nitric oxide and oxygen in vitro...
  71. pmc Superoxide-mediated amplification of the oxygen-induced switch from [4Fe-4S] to [2Fe-2S] clusters in the transcriptional regulator FNR
    Jason C Crack
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    Proc Natl Acad Sci U S A 104:2092-7. 2007
    ..This mechanism, a one electron activation of the cluster, coupled to catalytic recycling of the resulting superoxide ion back to oxygen, provides a means of amplifying the sensitivity of [4Fe-4S] FNR to its signal molecule...
  72. ncbi request reprint Mechanism of oxygen sensing by the bacterial transcription factor fumarate-nitrate reduction (FNR)
    Jason Crack
    School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich, NR4 7TJ
    J Biol Chem 279:9278-86. 2004
    ..Either the hydrogen peroxide formed on reaction with oxygen can be recycled by intracellular catalase or it can be used to oxidize further Fe-S clusters. In both cases, the efficacy of oxygen sensing by FNR will be increased...
  73. doi request reprint Influence of the environment on the [4Fe-4S]2+ to [2Fe-2S]2+ cluster switch in the transcriptional regulator FNR
    Jason C Crack
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich, NR4 7TJ, UK
    J Am Chem Soc 130:1749-58. 2008
    ..This work, which may have significance for the in vivo FNR cluster conversion reaction in the cell cytoplasm, provides an explanation for apparently contradictory results reported from different laboratories...
  74. pmc Three microarray platforms: an analysis of their concordance in profiling gene expression
    David Petersen
    Advanced Technology Center, Center for Cancer Research, National Cancer Institute, Gaithersburg, MD 20877, USA
    BMC Genomics 6:63. 2005
    ..As part of a validation study for the long oligonucleotide arrays, we compared and contrasted expression profiles from the three formats, testing RNA from six different cell lines against a universal reference standard...
  75. pmc Adaptation of the endogenous Salmonella enterica serovar Typhi clyA-encoded hemolysin for antigen export enhances the immunogenicity of anthrax protective antigen domain 4 expressed by the attenuated live-vector vaccine strain CVD 908-htrA
    James E Galen
    Center for Vaccine Development, Separtmrnt of Medicine, School of Medicine, University of Maryland, 685 W Baltimore St, Baltimore, MD 21201, USA
    Infect Immun 72:7096-106. 2004
    ..0002). In addition, the induction of PA-specific gamma interferon and interleukin 5 responses was observed in splenocytes. This technology offers exceptional versatility for enhancing the immunogenicity of bacterial live-vector vaccines...
  76. ncbi request reprint The double life of aconitase
    Peter J Artymiuk
    Structure 14:2-4. 2006