Timothy R Dafforn

Summary

Affiliation: University of Birmingham
Country: UK

Publications

  1. ncbi request reprint Linear dichroism of biomolecules: which way is up?
    Timothy R Dafforn
    Department of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Curr Opin Struct Biol 14:541-6. 2004
  2. pmc Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex
    Anita L Sullivan
    Department of Respiratory Medicine, University Hospitals Birmingham, Birmingham, UK
    Respir Res 9:60. 2008
  3. pmc So how do you know you have a macromolecular complex?
    Timothy R Dafforn
    Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, England
    Acta Crystallogr D Biol Crystallogr 63:17-25. 2007
  4. doi request reprint Tetramerization of ZapA is required for FtsZ bundling
    Raúl Pacheco-Gómez
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, West Midlands B15 2TT, UK
    Biochem J 449:795-802. 2013
  5. doi request reprint Detection of pathogenic bacteria using a homogeneous immunoassay based on shear alignment of virus particles and linear dichroism
    Raúl Pacheco-Gómez
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, West Midlands, B15 2TT, UK
    Anal Chem 84:91-7. 2012
  6. pmc Mutations of key hydrophobic surface residues of 11 beta-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression system
    Alexander J Lawson
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Protein Sci 18:1552-63. 2009
  7. pmc Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition
    Sarah Nicholls
    Cancer Research UK Institute for Cancer Studies, Edgbaston, Birmingham B15 2TT, United Kingdom
    Proc Natl Acad Sci U S A 106:3889-94. 2009
  8. pmc Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53
    Thomas H Millard
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, UK
    EMBO J 24:240-50. 2005
  9. pmc In vitro dimerization of the bovine papillomavirus E5 protein transmembrane domain
    Joanne Oates
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
    Biochemistry 47:8985-92. 2008
  10. ncbi request reprint Structure of a NHEJ polymerase-mediated DNA synaptic complex
    Nigel C Brissett
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK
    Science 318:456-9. 2007

Collaborators

  • David I Roper
  • Charles W Penn
  • Benjamin E Willcox
  • Klaus Futterer
  • David J Scott
  • A Rodger
  • Helen Parfrey
  • Guillaume Bompard
  • David A Lomas
  • Aiwu Zhou
  • Raúl Pacheco-Gómez
  • Matthew R Hicks
  • Rachel Marrington
  • Sarah Nicholls
  • Alexander J Lawson
  • Anita L Sullivan
  • Joanne Oates
  • Nigel C Brissett
  • Matthew Hicks
  • Thomas H Millard
  • David J Halsall
  • Clare Green
  • Corinne J I Smith
  • Xi Cheng
  • Stephen Addinall
  • Susan Stokoe
  • Hannah J England
  • John Ward
  • Emma Stanley
  • Julia Kraemer
  • Paul A H Moss
  • Premini Mahendra
  • Jonathan P Ride
  • Charles Craddock
  • Peter van Endert
  • Mahboob Salim
  • Mark Cobbold
  • Karen P Piper
  • Paul M Stewart
  • Scott A White
  • Elizabeth A Walker
  • Hansjorg Schild
  • Stefan Tenzer
  • Victor H Engelhard
  • Fiyaz Mohammed
  • Ann M Dixon
  • Pieter S Hiemstra
  • Robert A Stockley
  • Daniel DiMaio
  • Aidan J Doherty
  • Andrew J Green
  • Raquel Juarez
  • Luis Blanco
  • Robert S Pitcher
  • Angel J Picher
  • Gavin C Fox
  • Christopher M Thomas
  • Sarah M Batt
  • James I MacDonald
  • Laura M Machesky
  • Man Yeung Heung
  • Gemma Brown
  • David Gubb
  • Jean Marc Reichhart
  • Terri Morley

Detail Information

Publications17

  1. ncbi request reprint Linear dichroism of biomolecules: which way is up?
    Timothy R Dafforn
    Department of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Curr Opin Struct Biol 14:541-6. 2004
    ....
  2. pmc Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex
    Anita L Sullivan
    Department of Respiratory Medicine, University Hospitals Birmingham, Birmingham, UK
    Respir Res 9:60. 2008
    ..A fall in SLPI concentration is also seen in culture supernatants of respiratory cells exposed to NE, for unknown reasons. We investigated the hypothesis that SLPI complexed with NE associates with cell membranes in vitro...
  3. pmc So how do you know you have a macromolecular complex?
    Timothy R Dafforn
    Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, England
    Acta Crystallogr D Biol Crystallogr 63:17-25. 2007
    ..Techniques are discussed that will provide information on the stoichiometry of complexes as well as low-resolution information on complex structure. Together, these data will help to identify the physiological complex...
  4. doi request reprint Tetramerization of ZapA is required for FtsZ bundling
    Raúl Pacheco-Gómez
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, West Midlands B15 2TT, UK
    Biochem J 449:795-802. 2013
    ..We show that one of these mutants, I83E, is fully folded and binds to FtsZ, but is a constitutive dimer. Using this mutant we show that tetramerization is a requirement for both FtsZ bundling and GTPase modulation activities...
  5. doi request reprint Detection of pathogenic bacteria using a homogeneous immunoassay based on shear alignment of virus particles and linear dichroism
    Raúl Pacheco-Gómez
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, West Midlands, B15 2TT, UK
    Anal Chem 84:91-7. 2012
    ..The addition of the target ligand (in this case Escherichia coli O157) leads to the formation of a nanoparticle-ligand particle that is unable to align, leading to the perturbation of the linear dichroism signal...
  6. pmc Mutations of key hydrophobic surface residues of 11 beta-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression system
    Alexander J Lawson
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Protein Sci 18:1552-63. 2009
    ..Hence, the F278E mutants should be useful for many future biochemical and biophysical studies of the enzyme...
  7. pmc Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition
    Sarah Nicholls
    Cancer Research UK Institute for Cancer Studies, Edgbaston, Birmingham B15 2TT, United Kingdom
    Proc Natl Acad Sci U S A 106:3889-94. 2009
    ..Our results define the molecular mechanisms governing immunogenicity of HA-1, and highlight how single amino acid polymorphisms in mHags can critically affect both MHC association and TCR recognition...
  8. pmc Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53
    Thomas H Millard
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, UK
    EMBO J 24:240-50. 2005
    ..This study promotes an expanded view of IRSp53 as an actin regulator that integrates scaffolding and effector functions...
  9. pmc In vitro dimerization of the bovine papillomavirus E5 protein transmembrane domain
    Joanne Oates
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
    Biochemistry 47:8985-92. 2008
    ..0 kcal mol (-1). We have used our new results to interpret existing models of E5 dimer formation and provide a direct link between TM helix interactions and E5 function...
  10. ncbi request reprint Structure of a NHEJ polymerase-mediated DNA synaptic complex
    Nigel C Brissett
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK
    Science 318:456-9. 2007
    ....
  11. ncbi request reprint Looking at long molecules in solution: what happens when they are subjected to Couette flow?
    Alison Rodger
    Department of Chemistry, University of Warwick, Coventry, UKCV4 7AL
    Phys Chem Chem Phys 8:3161-71. 2006
    ....
  12. ncbi request reprint Restriction enzyme kinetics monitored by UV linear dichroism
    Matthew R Hicks
    Department of Chemistry, University of Warwick, Coventry, UK
    Biochemistry 45:8912-7. 2006
    ..LD, as implemented in this new assay, is broadly applicable across a wide range of DNA-modifying enzymes and compounds and, as such, is a useful addition to the toolbox of biological characterization...
  13. ncbi request reprint Validation of new microvolume Couette flow linear dichroism cells
    Rachel Marrington
    Department of Chemistry, University of Warwick, Coventry, UK CV4 7AL
    Analyst 130:1608-16. 2005
    ....
  14. pmc Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism
    Rachel Marrington
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    Biophys J 87:2002-12. 2004
    ..As with conventional fluorescence, improved sensitivity compared with absorbance LD is to be expected after instrumentation optimization...
  15. ncbi request reprint Physical characterization of serpin conformations
    Timothy R Dafforn
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
    Methods 32:150-8. 2004
    ..In this chapter, we will outline these general approaches that can be used routinely to identify the alternative serpin conformational states...
  16. ncbi request reprint Targeting a surface cavity of alpha 1-antitrypsin to prevent conformational disease
    Helen Parfrey
    Respiratory Medicine Unit, Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:33060-6. 2003
    ..These results demonstrate the importance of this cavity as a site for drug design to ameliorate polymerization and prevent the associated conformational disease...
  17. ncbi request reprint Drosophila necrotic mutations mirror disease-associated variants of human serpins
    Clare Green
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    Development 130:1473-8. 2003
    ..Taken together, these data establish Drosophila as a powerful system to study serpin polymerization in vivo...