Timothy R Dafforn
Affiliation: University of Birmingham
- Linear dichroism of biomolecules: which way is up?Timothy R Dafforn
Department of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
Curr Opin Struct Biol 14:541-6. 2004....
- Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complexAnita L Sullivan
Department of Respiratory Medicine, University Hospitals Birmingham, Birmingham, UK
Respir Res 9:60. 2008..A fall in SLPI concentration is also seen in culture supernatants of respiratory cells exposed to NE, for unknown reasons. We investigated the hypothesis that SLPI complexed with NE associates with cell membranes in vitro...
- So how do you know you have a macromolecular complex?Timothy R Dafforn
Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, England
Acta Crystallogr D Biol Crystallogr 63:17-25. 2007..Techniques are discussed that will provide information on the stoichiometry of complexes as well as low-resolution information on complex structure. Together, these data will help to identify the physiological complex...
- Tetramerization of ZapA is required for FtsZ bundlingRaúl Pacheco-Gómez
School of Biosciences, University of Birmingham, Edgbaston, Birmingham, West Midlands B15 2TT, UK
Biochem J 449:795-802. 2013..We show that one of these mutants, I83E, is fully folded and binds to FtsZ, but is a constitutive dimer. Using this mutant we show that tetramerization is a requirement for both FtsZ bundling and GTPase modulation activities...
- Detection of pathogenic bacteria using a homogeneous immunoassay based on shear alignment of virus particles and linear dichroismRaúl Pacheco-Gómez
School of Biosciences, University of Birmingham, Edgbaston, Birmingham, West Midlands, B15 2TT, UK
Anal Chem 84:91-7. 2012..The addition of the target ligand (in this case Escherichia coli O157) leads to the formation of a nanoparticle-ligand particle that is unable to align, leading to the perturbation of the linear dichroism signal...
- Mutations of key hydrophobic surface residues of 11 beta-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression systemAlexander J Lawson
School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
Protein Sci 18:1552-63. 2009..Hence, the F278E mutants should be useful for many future biochemical and biophysical studies of the enzyme...
- Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognitionSarah Nicholls
Cancer Research UK Institute for Cancer Studies, Edgbaston, Birmingham B15 2TT, United Kingdom
Proc Natl Acad Sci U S A 106:3889-94. 2009..Our results define the molecular mechanisms governing immunogenicity of HA-1, and highlight how single amino acid polymorphisms in mHags can critically affect both MHC association and TCR recognition...
- Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53Thomas H Millard
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, UK
EMBO J 24:240-50. 2005..This study promotes an expanded view of IRSp53 as an actin regulator that integrates scaffolding and effector functions...
- In vitro dimerization of the bovine papillomavirus E5 protein transmembrane domainJoanne Oates
Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
Biochemistry 47:8985-92. 2008..0 kcal mol (-1). We have used our new results to interpret existing models of E5 dimer formation and provide a direct link between TM helix interactions and E5 function...
- Structure of a NHEJ polymerase-mediated DNA synaptic complexNigel C Brissett
Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK
Science 318:456-9. 2007....
- Looking at long molecules in solution: what happens when they are subjected to Couette flow?Alison Rodger
Department of Chemistry, University of Warwick, Coventry, UKCV4 7AL
Phys Chem Chem Phys 8:3161-71. 2006....
- Restriction enzyme kinetics monitored by UV linear dichroismMatthew R Hicks
Department of Chemistry, University of Warwick, Coventry, UK
Biochemistry 45:8912-7. 2006..LD, as implemented in this new assay, is broadly applicable across a wide range of DNA-modifying enzymes and compounds and, as such, is a useful addition to the toolbox of biological characterization...
- Validation of new microvolume Couette flow linear dichroism cellsRachel Marrington
Department of Chemistry, University of Warwick, Coventry, UK CV4 7AL
Analyst 130:1608-16. 2005....
- Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroismRachel Marrington
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
Biophys J 87:2002-12. 2004..As with conventional fluorescence, improved sensitivity compared with absorbance LD is to be expected after instrumentation optimization...
- Physical characterization of serpin conformationsTimothy R Dafforn
School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
Methods 32:150-8. 2004..In this chapter, we will outline these general approaches that can be used routinely to identify the alternative serpin conformational states...
- Targeting a surface cavity of alpha 1-antitrypsin to prevent conformational diseaseHelen Parfrey
Respiratory Medicine Unit, Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
J Biol Chem 278:33060-6. 2003..These results demonstrate the importance of this cavity as a site for drug design to ameliorate polymerization and prevent the associated conformational disease...
- Drosophila necrotic mutations mirror disease-associated variants of human serpinsClare Green
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
Development 130:1473-8. 2003..Taken together, these data establish Drosophila as a powerful system to study serpin polymerization in vivo...