Genomes and Genes
Neil O Carragher
Affiliation: University of Glasgow
- A novel role for FAK as a protease-targeting adaptor protein: regulation by p42 ERK and SrcNeil O Carragher
The Beatson Institute for Cancer Research, Cancer Research United Kingdom, Beatson Laboratories, Glasgow G61 1BD, Scotland, United Kingdom
Curr Biol 13:1442-50. 2003..Furthermore, FAK is necessary for recruiting both Calpain 2 and p42ERK/MAPK to peripheral adhesion sites facilitating maximal Calpain activity...
- Focal adhesion and actin dynamics: a place where kinases and proteases meet to promote invasionNeil O Carragher
The Beatson Institute for Cancer Research, Cancer Research UK, Glasgow G61 1BD, UK
Trends Cell Biol 14:241-9. 2004..Dysregulation of these is associated with tumour cell invasion. In this article, we discuss established roles for these signalling pathways, as well as the functional interplay between them in controlling the migratory phenotype...
- v-Src-induced modulation of the calpain-calpastatin proteolytic system regulates transformationN O Carragher
The Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, Glasgow, Scotland, United Kingdom
Mol Cell Biol 22:257-69. 2002..Thus, modulation of the calpain-calpastatin proteolytic system plays an important role in focal adhesion disassembly, morphological transformation, and cell cycle progression during v-Src-induced cell transformation...
- Calpain activity is generally elevated during transformation but has oncogene-specific biological functionsN O Carragher
The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Glasgow G61 1BD, Scotland, UK
Neoplasia 6:53-73. 2004..Thus, targeting calpain activity may represent a useful general strategy for interfering with activated proto-oncogenes in cancer cells...
- Calpain: a role in cell transformation and migrationNeil O Carragher
The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, G61 1BD Glasgow, Scotland, UK
Int J Biochem Cell Biol 34:1539-43. 2002..Thus, recent investigations suggest that the role of calpain in promoting cell transformation and cell migration may have important in vivo consequences in the context of cancer pathobiology...
- Intravital FLIM-FRET imaging reveals dasatinib-induced spatial control of src in pancreatic cancerMax Nobis
The Beatson Institute for Cancer Research, Glasgow Section of Dermatology, School of Medicine, University of Glasgow, Glasgow, UK
Cancer Res 73:4674-86. 2013..We propose that using FLIM-FRET in this capacity could provide a useful preclinical tool in animal models before clinical translation...
- Dasatinib inhibits mammary tumour development in a genetically engineered mouse modelSaadia A Karim
Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK
J Pathol 230:430-40. 2013..Dasatinib also inhibited both the migration and the invasion of tumour-derived cell lines in vitro. Together these data support the argument that benefits of Src inhibitors may predominate in early or even pre-invasive disease...
- Advanced intravital subcellular imaging reveals vital three-dimensional signalling events driving cancer cell behaviour and drug responses in live tissueMax Nobis
The Beatson Institute for Cancer Research, Glasgow, UK
FEBS J 280:5177-97. 2013..Furthermore, we offer a prospective view of how imaging applications may be integrated further with the aim of understanding disease in a more physiological and functional manner within the framework of the drug discovery process...
- v-Src's hold over actin and cell adhesionsMargaret C Frame
The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK
Nat Rev Mol Cell Biol 3:233-45. 2002..Recent experiments have defined some of the key targets and effector pathways that mediate the pleiotropic oncogenic effects of v-Src...
- AP-1 differentially expressed proteins Krp1 and fibronectin cooperatively enhance Rho-ROCK-independent mesenchymal invasion by altering the function, localization, and activity of nondifferentially expressed proteinsHeather J Spence
Invasion and Metastasis Laboratory, Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, United Kingdom
Mol Cell Biol 26:1480-95. 2006....
- The mechanism of cell cycle regulation by v-SrcD Riley
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK
Oncogene 20:5941-50. 2001..However, the MEK inhibitor prevented p27 protein down regulation whereas the PI3-K inhibitor did not. This was because reduced PI3-K activity lead to proteolytic degradation of p27...
- The role of focal-adhesion kinase in cancer - a new therapeutic opportunityGordon W McLean
The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate Switchback Road, Bearsden, Glasgow G61 1BD, United Kingdom
Nat Rev Cancer 5:505-15. 2005..Mouse models have shown that FAK is involved in tumour formation and progression, and other studies showing that FAK expression is increased in human tumours make FAK a potentially important new therapeutic target...
- SRC-mediated phosphorylation of focal adhesion kinase couples actin and adhesion dynamics to survival signalingM A Westhoff
The Beatson Institute for Cancer Research, Cancer Research UK, Glasgow G61 1BD, Scotland, United Kingdom
Mol Cell Biol 24:8113-33. 2004..This also highlights that dynamic regulation of actin and adhesions (which include the integrin matrix receptors) is critical to signaling output and biological responses...
- Spatial regulation of RhoA activity during pancreatic cancer cell invasion driven by mutant p53Paul Timpson
The Beatson Institute for Cancer Research, Garscube Estate, Glasgow, United Kingdom
Cancer Res 71:747-57. 2011....
- Calpain inhibition: a therapeutic strategy targeting multiple disease statesN O Carragher
The Beaston Insititue for Cancer Research, Cancer Research UK, Glasgow G61 1BD, Scotland, UK
Curr Pharm Des 12:615-38. 2006..The development of a new class of calpain inhibitors that interact with domains outside of the catalytic site of calpain may provide greater specificity and therapeutic potential...
- Calpain 2 and Src dependence distinguishes mesenchymal and amoeboid modes of tumour cell invasion: a link to integrin functionN O Carragher
The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Glasgow, UK
Oncogene 25:5726-40. 2006..Thus, the need for a major pathway that controls integrin adhesion turnover defines and distinguishes cancer cell invasion strategies...
- Cleavage of focal adhesion kinase by different proteases during SRC-regulated transformation and apoptosis. Distinct roles for calpain and caspasesN O Carragher
The Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, Glasgow G61 1BD, United Kingdom
J Biol Chem 276:4270-5. 2001..Thus, calpain and caspase differentially regulate focal adhesion turnover during Src-regulated cell transformation, motility, and apoptosis...
- Inhibition of calpain stabilises podosomes and impairs dendritic cell motilityYolanda Calle
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, UK
J Cell Sci 119:2375-85. 2006..In summary, our results provide evidence that calpain regulates podosome composition and turnover and that this process is required for efficient migration of dendritic cells...
- Assaying calpain activityNeil O Carragher
Cell Adhesion Linked Kinase Laboratory, Beatson Institute for Cancer Research, Bearsden, Glassgow, UK
Methods Mol Biol 370:109-20. 2007..The development of such second-generation assays may help to identify more effective calpain intervention strategies...
- Role of discoidin domain receptors 1 and 2 in human smooth muscle cell-mediated collagen remodeling: potential implications in atherosclerosis and lymphangioleiomyomatosisNicola Ferri
Department of Pathology, University of Washington School of Medicine, Seattle, Washington, USA
Am J Pathol 164:1575-85. 2004..Taken together, these data provide evidence for the potential roles of DDR1 and DDR2 in the regulation of collagen turnover mediated by SMCs in obstructive diseases of blood vessels and the lung...