Robert A Brown
Affiliation: University College London
- In the beginning there were soft collagen-cell gels: towards better 3D connective tissue models?Robert A Brown
University College London, UCL Centre for Tissue Regeneration Science, Institute of Orthopaedics, Division of Surgery, RNOH, Stanmore Campus, London, HA7 4LP UK Electronic address
Exp Cell Res 319:2460-9. 2013..It is concluded that across this 40 year window major progress has been made towards establishing a family of 3D experimental collagen tissue-models, suitable to investigate normal and pathological fibroblast mechano-functions...
- The origins and regulation of tissue tension: identification of collagen tension-fixation process in vitroMassimo Marenzana
University College London, RFUCMS, Tissue Repair and Engineering Centre, Institute of Orthopaedics, RNOH, Stanmore Campus, London HA7 4LP, UK
Exp Cell Res 312:423-33. 2006..Through this model, we have been able to identify the 'tension remodeling' process, by which cells control material properties in response to environmental factors...
- Guiding cell migration in 3D: a collagen matrix with graded directional stiffnessEktoras Hadjipanayi
University College London, Tissue Repair and Engineering Centre, Institute of Orthopaedics, United Kingdom
Cell Motil Cytoskeleton 66:121-8. 2009..Durotactic migration was significant after 6 days. This model provides a new means for studying cellular mechanotaxis and patterning cells which is controllable, biomimetic and in 3D...
- Fluid shear in viscous fibronectin gels allows aggregation of fibrous materials for CNS tissue engineeringJames B Phillips
Tissue Repair and Engineering Centre, University College London, London, UK
Biomaterials 25:2769-79. 2004..This was extended using an impermeable coating to improve orientation and straightness of axonal growth...
- Hyaluronan hydration generates three-dimensional meso-scale structure in engineered collagen tissuesNelomi Anandagoda
UCL Institute of Orthopaedics and Musculoskeletal Science, UCL Tissue Repair and Engineering Centre, University College London, Stanmore Campus, London HA7 4LP, UK
J R Soc Interface 9:2680-7. 2012..These constructs may have potential as functional in vitro models representing developmental and pathological processes...
- Mechanical anisotropy in compressed collagen produced by localised photodynamic cross-linkingJosephine P F Wong
University College London, UCL Tissue Repair and Engineering Centre, Institute of Orthopaedics, Division of Surgery and Interventional Science, University College London, HA7 4LP, UK
J Mech Behav Biomed Mater 18:132-9. 2013..The increase in bonding strength between layers after cross-linking enhances layer integration and graded surface stiffness will impact on cellular/mechanical properties of compressed gels...
- Identification of key factors in deep O2 cell perfusion for vascular tissue engineeringUmber Cheema
University College London, Division of Surgical and Interventional Sciences, Institute of Orthopaedics and Musculoskeletal Sciences, Tissue Repair and Engineering Centre, Stanmore Campus, London, UK
Int J Artif Organs 32:318-28. 2009..05), largely eliminating the problem. This study has identified key parameters dictating vascular smooth muscle cell behavior in 3D engineered tissue culture...
- Evidence for sequential utilization of fibronectin, vitronectin, and collagen during fibroblast-mediated collagen contractionKamaljit K Sethi
University College London, RFUCMS, Tissue Repair and Engineering Center, Institute of Orthopaedics, RNOH Campus, Stanmore, United Kingdom
Wound Repair Regen 10:397-408. 2002..We propose that there is a short cascade of sequential integrin-ligand interactions as cells attach to, extend through, and eventually contract their matrix. (WOUND REP REG 2002;10:-408)..
- A rapid fabricated living dermal equivalent for skin tissue engineering: an in vivo evaluation in an acute wound modelMichael Ananta
Institute of Orthopaedics, University College London, Stanmore, Middlesex, United Kingdom
Tissue Eng Part A 18:353-61. 2012..It is therefore proposed that the presented method could reduce the high cost associated with the production of living, tissue-engineered dressings...
- Bulk collagen incorporation rates into knitted stiff fibre polymer in tissue-engineered scaffolds: the rate-limiting stepFarhad Foroughi
University College London UCL, Tissue Repair and Engineering Centre, Institute of Orthopaedics, London, UK
J Tissue Eng Regen Med 2:507-14. 2008..e. deposited) within the construct during culture. This may be enhanced by systems which promote or assemble true 3D multi-layers of cells...
- Matrix mechanical properties of transversalis fascia in inguinal herniation as a model for tissue expansionAlvena Kureshi
University College London, Tissue Repair and Engineering Centre, Institute of Orthopaedics, Stanmore Campus, London HA7 4LP, UK
J Biomech 41:3462-8. 2008..These findings provide new clues to collagen tissue herniation for mathematical modelling and model tissue engineering...
- Cell responses to biomimetic protein scaffolds used in tissue repair and engineeringRobert A Brown
Tissue Regeneration and Engineering Center, Institute of Orthopedics, University College London, Stanmore Campus, London, HA7 4LP, United Kingdom
Int Rev Cytol 262:75-150. 2007....
- Investigating mechanical behaviour at a core-sheath interface in peripheral nerveRachel L Tillett
Tissue Repair and Engineering Centre, University College London, London, UK
J Peripher Nerv Syst 9:255-62. 2004....
- Direct collagen-material engineering for tissue fabricationRobert A Brown
Division of Surgery, UCL Centre for Tissue Regeneration Science, Institute of Orthopaedics, University College London, London, United Kingdom
Tissue Eng Part A 19:1495-8. 2013..This phrase explains very little of the substance and gets nowhere near to the base of the question. We need to be clear about why this is important enough to justify a major new research investment...
- Development of conical soluble phosphate glass fibers for directional tissue growthTijna Alekseeva
Tissue Repair and Engineering Centre, Institute of Orthopaedics, University College London, Stanmore Campus, London HA7 4LP, UK
J Biomater Appl 26:733-44. 2012..a mean rate of 0.5 µm/mm (n=20) change. These conically shaped fibers can also be manipulated and potentially used to promote uniaxial cell-tissue ingrowth for improved innervations and vascularization of tissue engineered constructs...
- Stabilisation of cables of fibronectin with micromolar concentrations of copper: in vitro cell substrate propertiesZubair Ahmed
University College London, Tissue Repair and Engineering Centre, Institute of Orthopaedics, RNOH Campus, Brockley Hill, HA7 4LP Stanmore, UK
Biomaterials 25:803-12. 2004..These results could be used to design biomaterials with optimum properties for promoting cellular ingrowth and survival in tissue engineered grafts which may be used to improve peripheral nerve repair...
- Enhanced fibroblast contraction of 3D collagen lattices and integrin expression by TGF-beta1 and -beta3: mechanoregulatory growth factors?Robert A Brown
Tissue Repair Unit, Centre for Plastic and Reconstructive Surgery, University College London, RFUCMS, 67 Riding House Street, London, W1W 7EJ, United Kingdom
Exp Cell Res 274:310-22. 2002..It is concluded that TGF-beta1 and -beta3 behave here primarily as mechanoregulatory growth factors and that stimulation of integrin expression may be a consequence of the altered cell stress...
- Roofed grooves: rapid layer engineering of perfusion channels in collagen tissue modelsNoah S Tan
Tissue Repair and Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom
J Biomater Appl 29:605-16. 2014..The results of this study provide a valuable platform for rapid fabrication of complex collagen-based tissues in particular for provision of perfusing microchannels through the bulk material for improved core nutrient supply. ..
- Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture modelAmir G Ardakani
University College London, Tissue Repair and Engineering Centre, Institute for Orthopaedics and Musculoskeletal Sciences, Stanmore Campus, London HA7 4LP, UK
J R Soc Interface 11:20140501. 2014....
- Pre-crosslinked polymeric collagen in 3-D models of mechanically stiff tissues: blended collagen polymer hydrogels for rapid layer fabricationJosephine P F Wong
UCL Institute of Orthopaedics and Musculoskeletal Sciences, UCL Tissue Repair and Engineering Centre, University College London, Stanmore Campus, London HA7 4LP, UK
Acta Biomater 10:5005-11. 2014..This is the first report of native polymeric collagen used in a tissue engineering context, for the rapid production of a stiff collagen-cell constructs. ..
- Characterization of a "blanch-blush" mechano-response in palmar skinThomas C Wright
Tissue Repair and Engineering Centre, Institute of Orthopaedics and Musculoskeletal Science, University College London, London, UK
J Invest Dermatol 126:220-6. 2006..It represents an elegant demonstration of collagenous tissue function through an everyday tissue reaction, which has not been described previously...