S C Andrews

Summary

Affiliation: University of Sheffield
Country: UK

Publications

  1. pmc Haem and non-haem iron sites in Escherichia coli bacterioferritin: spectroscopic and model building studies
    M R Cheesman
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, U K
    Biochem J 292:47-56. 1993
  2. ncbi request reprint A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system
    S C Andrews
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:3633-47. 1997
  3. pmc Ferritin mutants of Escherichia coli are iron deficient and growth impaired, and fur mutants are iron deficient
    H Abdul-Tehrani
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Bacteriol 181:1415-28. 1999
  4. pmc Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli
    S J Davies
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Bacteriol 181:5624-35. 1999
  5. ncbi request reprint Overproduction, purification and characterization of the bacterioferritin of Escherichia coli and a C-terminally extended variant
    S C Andrews
    Krebs Institute of Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, England
    Eur J Biochem 213:329-38. 1993
  6. ncbi request reprint Structure-function relationships in the ferritins
    P M Harrison
    Krebs Institute, University of Sheffield, UK
    Met Ions Biol Syst 35:435-77. 1998
  7. ncbi request reprint Direct observation of the iron binding sites in a ferritin
    P D Hempstead
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 350:258-62. 1994
  8. ncbi request reprint Bacterioferritins and ferritins are distantly related in evolution. Conservation of ferroxidase-centre residues
    S C Andrews
    Krebs Institute for Biomolecular Research, University of Sheffield, Western Bank, UK
    FEBS Lett 293:164-8. 1991
  9. pmc Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12
    S C Andrews
    Krebs Institute, Department of Molecular Biology, University of Sheffield, United Kingdom
    J Bacteriol 171:3940-7. 1989
  10. ncbi request reprint A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map
    S C Andrews
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    J Gen Microbiol 137:361-7. 1991

Collaborators

Detail Information

Publications35

  1. pmc Haem and non-haem iron sites in Escherichia coli bacterioferritin: spectroscopic and model building studies
    M R Cheesman
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, U K
    Biochem J 292:47-56. 1993
    ..293, 164-168] and the possibility that this Fe(III) ion may reside at or near the ferroxidase centre is discussed...
  2. ncbi request reprint A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system
    S C Andrews
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:3633-47. 1997
    ..It is likely that HyfR acts as a formate-dependent regulator of the hyf operon and that FocB provides the Hyf complex with external formate as substrate...
  3. pmc Ferritin mutants of Escherichia coli are iron deficient and growth impaired, and fur mutants are iron deficient
    H Abdul-Tehrani
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Bacteriol 181:1415-28. 1999
    ..In addition to repressing the iron acquisition systems, Fur appears to regulate the demand for iron, probably by controlling the expression of iron-containing proteins. The role of Bfr remains unclear...
  4. pmc Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli
    S J Davies
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Bacteriol 181:5624-35. 1999
    ..Reverse transcriptase-mediated primer extension indicated a single transcriptional start site centered 81 bp downstream of a strongly predicted CRP-binding site...
  5. ncbi request reprint Overproduction, purification and characterization of the bacterioferritin of Escherichia coli and a C-terminally extended variant
    S C Andrews
    Krebs Institute of Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, England
    Eur J Biochem 213:329-38. 1993
    ..A combination of Mössbauer spectroscopy and electron diffraction showed that the BFR loaded with iron in vitro has a ferrihydrite-like iron core, whereas the in-vivo loaded protein has an amorphous core...
  6. ncbi request reprint Structure-function relationships in the ferritins
    P M Harrison
    Krebs Institute, University of Sheffield, UK
    Met Ions Biol Syst 35:435-77. 1998
  7. ncbi request reprint Direct observation of the iron binding sites in a ferritin
    P D Hempstead
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 350:258-62. 1994
    ..The third iron is bound by ligands unique to Ec-FTN on the inner surface of the protein shell. It is speculated that this state may represent the nucleation centre of a novel type of Fe(III) cluster, recently observed in Ec-FTN...
  8. ncbi request reprint Bacterioferritins and ferritins are distantly related in evolution. Conservation of ferroxidase-centre residues
    S C Andrews
    Krebs Institute for Biomolecular Research, University of Sheffield, Western Bank, UK
    FEBS Lett 293:164-8. 1991
    ..Optimum alignment of bacterioferritin and ferritin sequences suggests that key residues of the ferroxidase centres of ferritins are conserved in bacterioferritins...
  9. pmc Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12
    S C Andrews
    Krebs Institute, Department of Molecular Biology, University of Sheffield, United Kingdom
    J Bacteriol 171:3940-7. 1989
    ..An additional unidentified gene (gen-64), encoding a relatively basic 64-residue polypeptide and having the same polarity as bfr, was detected upstream of the bfr gene...
  10. ncbi request reprint A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map
    S C Andrews
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    J Gen Microbiol 137:361-7. 1991
    ..The translation product of orf3 (205 amino acid codons) resembled the iron-sulphur protein component (DMS B subunit) of the anaerobic dimethylsulphoxide reductase complex of E. coli...
  11. ncbi request reprint Overproduction, purification and characterization of the Escherichia coli ferritin
    A J Hudson
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, England
    Eur J Biochem 218:985-95. 1993
    ..E. coli is thus an organism containing both a ferritin and a bacterioferritin and the relative roles of the two iron-storage proteins are discussed in this study...
  12. ncbi request reprint Iron acquisition and virulence in Helicobacter pylori: a major role for FeoB, a high-affinity ferrous iron transporter
    J Velayudhan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 37:274-86. 2000
    ..pylori Fe acquisition. feoB mutants were unable to colonize the gastric mucosa of mice, indicating that FeoB makes an important contribution to Fe acquisition by H. pylori in the low-pH, low-O2 environment of the stomach...
  13. ncbi request reprint Topological analysis of DctQ, the small integral membrane protein of the C4-dicarboxylate TRAP transporter of Rhodobacter capsulatus
    N R Wyborn
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, S10 2TN, UK
    FEMS Microbiol Lett 194:13-7. 2001
    ..The data have provided a topological model which can be used to test hypotheses concerning the function of the different regions of DctQ and which can be applied to other members of the DctQ family...
  14. ncbi request reprint Site-directed replacement of the coaxial heme ligands of bacterioferritin generates heme-free variants
    S C Andrews
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom
    J Biol Chem 270:23268-74. 1995
    ..This study is consistent with the reported location of the BFR heme site at the 2-fold axis and shows that heme is unnecessary for BFR assembly and iron uptake...
  15. ncbi request reprint Amino acid sequence of the bacterioferritin (cytochrome b1) of Escherichia coli-K12
    S C Andrews
    Krebs Institute, Department of Molecular Biology and Biotechnology, University, Sheffield, UK
    Biochem Biophys Res Commun 158:489-96. 1989
    ..The fully assembled bacterioferritin molecule comprising 24 identical subunits and 12 haem moieties is a tetracosamer with an Mr of approximately 452,000...
  16. ncbi request reprint Construction and properties of Escherichia coli mutants defective in two genes encoding homologous membrane proteins with putative roles in anaerobic C4-dicarboxylic acid transport
    S Six
    Department of Molecular Biology and Biotechnology, University of Sheffield, U K
    Biochem Soc Trans 21:342S. 1993
  17. ncbi request reprint Spectroscopic and voltammetric characterisation of the bacterioferritin-associated ferredoxin of Escherichia coli
    M A Quail
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom
    Biochem Biophys Res Commun 229:635-42. 1996
    ..Bfd may thus be a 2Fe ferredoxin participating either in release/delivery of iron from/to bacterioferritin (or other iron complexes), or in iron-dependent regulation of bfr expression...
  18. ncbi request reprint Physical, chemical and immunological properties of the bacterioferritins of Escherichia coli, Pseudomonas aeruginosa and Azotobacter vinelandii
    S C Andrews
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, U K
    Biochim Biophys Acta 1078:111-6. 1991
    ..The results indicate that the bacterioferritins form a family of proteins that are distinct from the ferritins of plants and animals...
  19. ncbi request reprint The haemoglobin-like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases
    S C Andrews
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 302:247-52. 1992
    ..Structure-function properties of other FNR-like proteins, including LuxG and VanB, are also discussed...
  20. pmc Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli
    P Golby
    The School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, United Kingdom
    J Bacteriol 181:1238-48. 1999
    ..Although all three proteins have similar topologies and functions, and all are members of the two-component sensor-kinase family, their periplasmic domains appear to have evolved independently...
  21. pmc Transcriptional regulation and organization of the dcuA and dcuB genes, encoding homologous anaerobic C4-dicarboxylate transporters in Escherichia coli
    P Golby
    The School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, United Kingdom
    J Bacteriol 180:6586-96. 1998
    ..The expression of dcuB is entirely consistent with a primary role for DcuB in mediating C4-dicarboxylate transport during anaerobic fumarate respiration. The precise physiological purpose of DcuA remains unclear...
  22. ncbi request reprint The high-resolution X-ray crystallographic structure of the ferritin (EcFtnA) of Escherichia coli; comparison with human H ferritin (HuHF) and the structures of the Fe(3+) and Zn(2+) derivatives
    T J Stillman
    The Krebs Institute Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK
    J Mol Biol 307:587-603. 2001
    ..66 A) given the low amino acid sequence identity (22 %). Many of the conserved residues are clustered at the dinuclear centre but there is very little conservation of residues making inter-subunit interactions...
  23. ncbi request reprint Genetic and structural characterization of the bacterioferritin of Escherichia coli
    S C Andrews
    Krebs Institute for Biomolecular Research, University of Sheffield, U K
    Biochem Soc Trans 18:658-9. 1990
  24. pmc Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12
    P J Bell
    Department of Microbiology, University of Sheffield, United Kingdom
    J Bacteriol 171:3494-503. 1989
    ....
  25. pmc Identification of the ferroxidase centre of Escherichia coli bacterioferritin
    N E Le Brun
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, U K
    Biochem J 312:385-92. 1995
    ..The mechanism of iron (II) oxidation is discussed in the light of this and previous work...
  26. pmc Escherichia coli possesses two homologous anaerobic C4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct)
    S Six
    Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg Universitat, Mainz, Germany
    J Bacteriol 176:6470-8. 1994
    ..The anaerobic dicarboxylate transport systems (Dcu) are genetically and functionally distinct from the aerobic system (Dct)...
  27. ncbi request reprint Spectroscopic studies of cobalt(II) binding to Escherichia coli bacterioferritin
    A M Keech
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, United Kingdom
    J Biol Chem 272:422-9. 1997
    ..Discrimination against oxidation of single iron(II) ions avoids odd electron reduction products of oxygen...
  28. pmc Nucleotide sequence of the gene encoding the GMP reductase of Escherichia coli K12
    S C Andrews
    Department of Microbiology, University of Sheffield, U K
    Biochem J 255:35-43. 1988
    ....
  29. ncbi request reprint Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin
    N E Le Brun
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, UK
    FEBS Lett 333:197-202. 1993
    ....
  30. ncbi request reprint Iron storage in bacteria
    S C Andrews
    School of Animal and Microbial Sciences, University of Reading, UK
    Adv Microb Physiol 40:281-351. 1998
    ..No phenotype has been determined for E. coli bacterioferritin mutants and the precise role of bacterioferritin in E. coli remains uncertain...
  31. ncbi request reprint An EPR investigation of non-haem iron sites in Escherichia coli bacterioferritin and their interaction with phosphate. A study using nitric oxide as a spin probe
    N E Le Brun
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, UK
    FEBS Lett 323:261-6. 1993
    ..Under certain conditions, an iron dimer was also observed. The reaction of phosphate with NHI species has been investigated. Results point to a function for this anion in core nucleation...
  32. pmc Topological analysis of DcuA, an anaerobic C4-dicarboxylate transporter of Escherichia coli
    P Golby
    School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AJ, United Kingdom
    J Bacteriol 180:4821-7. 1998
    ..The topological model obtained for DcuA can be applied to DcuA homologues in other bacteria as well as to DcuB. Overproduction of DcuA to 15% of inner-membrane protein was obtained with the lacUV5-promoter-based plasmid, pYZ4...
  33. pmc Interaction of nitric oxide with non-haem iron sites of Escherichia coli bacterioferritin: reduction of nitric oxide to nitrous oxide and oxidation of iron(II) to iron(III)
    N E Le Brun
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, U K
    Biochem J 326:173-9. 1997
    ..This is consistent with a proposal that the diferric form of the centre is unstable and breaks down to form mononuclear iron species...
  34. ncbi request reprint Pumping iron: does bacterioferritin contain a redox-driven iron pump?
    A J Thomson
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, UK
    Biochem Soc Trans 25:96-101. 1997
  35. ncbi request reprint Isolation of a ferritin from Bacteroides fragilis
    E R Rocha
    University Department of Bacteriology and Immunology, Western Infirmary, Glasgow, UK
    FEMS Microbiol Lett 74:207-12. 1992
    ..This represents the first isolation of a ferritin protein from bacteria. It may alleviate iron toxicity in the presence of oxygen...