Affiliation: St George's
- A mysterious channel: new insights into proton channel functioning raise new questionsClaudia Eder
St George s, University of London, Division of Basic Medical Sciences, London SW17 0RE, UK
J Physiol 586:2419-20. 2008
- Ion channels in monocytes and microglia/brain macrophages: promising therapeutic targets for neurological diseasesClaudia Eder
Division of Basic Medical Sciences, St George s, University of London, Cranmer Terrace, London SW17 0RE, United Kingdom
J Neuroimmunol 224:51-5. 2010..Thus, ion channels of monocytes and microglia/brain macrophages could represent good candidates for therapeutic interventions in neurological diseases...
- Mechanisms of interleukin-1beta releaseClaudia Eder
Division of Basic Medical Sciences, St George s, University of London, Cranmer Terrace, Tooting, London SW17 0RE, UK
Immunobiology 214:543-53. 2009..A better understanding of IL-1beta release mechanisms is of great therapeutic relevance and may help in the development of strategies aimed at reducing the severity of inflammatory and autoimmune diseases...
- Importance of lipid rafts for lysophosphatidylcholine-induced caspase-1 activation and reactive oxygen species generationTom Schilling
Division of Biomedical Sciences, St George s University of London, Cranmer Terrace, London SW17 0RE, United Kingdom
Cell Immunol 265:87-90. 2010..Since ROS regulate caspase-1 activity in LPC-stimulated microglia, the effects of lipid raft-disrupting agents on caspase-1 activation can be related to their inhibition of NADPH oxidase-mediated ROS production...
- Importance of the non-selective cation channel TRPV1 for microglial reactive oxygen species generationTom Schilling
Division of Basic Medical Sciences, St George s, University of London, London, UK
J Neuroimmunol 216:118-21. 2009..Together, our data suggest that TRPV1 channels are involved in regulating NADPH oxidase-mediated ROS generation in microglia...
- Stimulus-dependent requirement of ion channels for microglial NADPH oxidase-mediated production of reactive oxygen speciesTom Schilling
Division of Basic Medical Sciences, St George s, University of London, London, United Kingdom
J Neuroimmunol 225:190-4. 2010..In contrast, activity of all four ion channel types was required for PMA-induced NADPH oxidase-mediated ROS generation, suggesting a differential, stimulus-dependent regulation of microglial ROS production by ion channel activity...
- Sodium dependence of lysophosphatidylcholine-induced caspase-1 activity and reactive oxygen species generationTom Schilling
Division of Basic Medical Sciences, St George s, University of London, Cranmer Terrace, London SW17 0RE, UK
Immunobiology 216:118-25. 2011..In summary, it is suggested that in LPC-activated microglia, Na(+) influx is required for the production of NADPH oxidase-mediated ROS, which subsequently stimulate caspase-1 activity...
- Fluorescence imaging of intracellular Ca2+, Na+, and H+ in cultured microgliaTom Schilling
St George s, University of London, London, UK
Methods Mol Biol 1041:147-61. 2013..This chapter summarizes protocols of loading of microglial cells with small-molecule ion indicators as well as protocols optimal for measurement and analysis of intracellular Ca(2+), Na(+), and H(+) concentrations in microglia in vitro. ..
- Patch clamp protocols to study ion channel activity in microgliaTom Schilling
St George s, University of London, London, UK
Methods Mol Biol 1041:163-82. 2013..This chapter summarizes patch clamp protocols optimal for recording and analysis of microglial ion channel activity in vitro and in situ...
- Lysophosphatidylcholine- and MCP-1-induced chemotaxis of monocytes requires potassium channel activityTom Schilling
Division of Basic Medical Sciences, St George s University of London, Cranmer Terrace, London, SW17 0RE, UK
Pflugers Arch 459:71-7. 2009..Thus, K+ channel inhibition may represent a novel powerful strategy to reduce monocyte infiltration and subsequent inflammation in atherosclerosis...
- Non-selective cation channel activity is required for lysophosphatidylcholine-induced monocyte migrationTom Schilling
Division of Basic Medical Sciences, St George s, University of London, Cranmer Terrace, London, UK
J Cell Physiol 221:325-34. 2009..Thus, ion channel inhibition may represent a powerful strategy to attenuate the progression of atherosclerosis by reducing monocyte infiltration...
- Microglial K(+) channel expression in young adult and aged miceTom Schilling
Institute for Infection and Immunity, St George s, University of London Cranmer Terrace, London, SW17 0RE, United Kingdom
Glia 63:664-72. 2015..It is concluded that age-related changes in microglial phenotype are accompanied by changes in the expression of microglial voltage-activated, but not Ca(2+) -activated, K(+) channels...
- TRPM7 regulates proliferation and polarisation of macrophagesTom Schilling
Infection and Immunity Research Institute, St George s, University of London, London SW17 0RE, UK
J Cell Sci 127:4561-6. 2014..In summary, our data suggest a main role of TRPM7 in the regulation of macrophage proliferation and polarisation. ..
- TRAM-34 inhibits nonselective cation channelsTom Schilling
Institute of Physiology, Medical Faculty Charité, Tucholskystrasse 2, 10117 Berlin, Germany
Pflugers Arch 454:559-63. 2007..These data indicate that TRAM-34 may cause additional effects on immune cells that are unrelated to the well-described inhibition of Ca(2+)-activated K(+) channels...
- Lysophosphatidylcholine stimulates IL-1beta release from microglia via a P2X7 receptor-independent mechanismChristian Stock
Institute of Physiology II, University of Muenster, Muenster, Germany
J Immunol 177:8560-8. 2006..In summary, these data indicate that the activity of nonselective cation channels and Ca(2+)-activated K(+) channels is required for optimal IL-1beta release from LPC-stimulated microglia...
- Regulation of microglial behavior by ion channel activityClaudia Eder
Institute of Physiology, Humboldt University, Berlin, Germany
J Neurosci Res 81:314-21. 2005....
- Functional importance of Ca2+-activated K+ channels for lysophosphatidic acid-induced microglial migrationTom Schilling
Institute of Physiology, Humboldt University Berlin, Tucholsky Strasse 2, D 10117 Berlin, Germany
Eur J Neurosci 19:1469-74. 2004..Microglial migration was not inhibited by 5 micro m paxilline. It is concluded that IKCa1 Ca(2+)-activated K(+) channels are required for LPA-stimulated migration of microglial cells...
- Physiological mechanisms of lysophosphatidylcholine-induced de-ramification of murine microgliaTom Schilling
Institute of Physiology, Humboldt University, Tucholskystr 2, D 10117 Berlin, Germany
J Physiol 557:105-20. 2004..LPC-induced microglial de-ramification was prevented by simultaneous inhibition of non-selective cation channels and K(+)-Cl(-) cotransporters, suggesting their functional importance for microglial activation...
- Voltage-activated proton currents in human lymphocytesTom Schilling
Institute of Physiology, Humboldt University, Tucholskystrasse 2, D 10117 Berlin, Germany
J Physiol 545:93-105. 2002..The pattern of expression of H(+) channels in lymphocytes appears well suited to their proposed role of charge compensation during the respiratory burst...