E D Williamson
Affiliation: Porton Down
- A single dose sub-unit vaccine protects against pneumonic plagueE D Williamson
DERA Chemical and Biological Defence Sector, Porton Down, Wiltshire SP4 0JQ, Salisbury, UK
Vaccine 19:566-71. 2000..The enhanced protective efficacy of this sub-unit vaccine administered as a single dose compared with an existing vaccine has been demonstrated in an outbred animal model of pneumonic plague...
- Human immune response to a plague vaccine comprising recombinant F1 and V antigensE D Williamson
Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
Infect Immun 73:3598-608. 2005..Potential serological immune correlates of protection have been investigated, but no trends specific to vaccination could be detected in cellular markers...
- Immunisation against plague by transcutaneous and intradermal application of subunit antigensJ E Eyles
Biomedical Sciences, Dstl, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
Vaccine 22:4365-73. 2004..These data suggest that transcutaneous immunisation may be a simple and non-invasive method for immunising individuals against plague...
- Regions of Yersinia pestis V antigen that contribute to protection against plague identified by passive and active immunizationJ Hill
Microbiology, CBD Porton Down, Salisbury, Wiltshire, United Kingdom
Infect Immun 65:4476-82. 1997....
- Kinetics of the immune response to the (F1+V) vaccine in models of bubonic and pneumonic plagueE D Williamson
Dstl Porton Down, Salisbury, Wilts SP4 0JQ, UK
Vaccine 25:1142-8. 2007....
- Protective efficacy of a fully recombinant plague vaccine in the guinea pigS M Jones
Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
Vaccine 21:3912-8. 2003..Cross-protection of the mouse with guinea pig IgG suggests that the recognition of neutralising epitopes in the F1 and V proteins is conserved between these two species...
- Immunogenicity of recombinant protective antigen and efficacy against aerosol challenge with anthraxE D Williamson
Defence Science and Technology Laboratory Porton Down, Salisbury, Wilts SP4 0JQ, United Kingdom
Infect Immun 73:5978-87. 2005..anthracis. These data provide some preliminary evidence for the existence of immune correlates of protection against anthrax infection in rhesus macaques immunized with rPA...
- A biocompatible microdevice for core body temperature monitoring in the early diagnosis of infectious diseaseE D Williamson
Dstl Porton Down, Salisbury, Wilts, UK, SP4 0JQ
Biomed Microdevices 9:51-60. 2007....
- Mouse model characterisation for anthrax vaccine development: comparison of one inbred and one outbred mouse strainH C Flick-Smith
Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
Microb Pathog 38:33-40. 2005..An assessment of protection in the TO mouse against aerosol challenge with the fully virulent strain of B. anthracis, Ames, was also made...
- Immunogenicity of the rF1+rV vaccine for plague with identification of potential immune correlatesE D Williamson
Defence Science and Technology Laboratory, Porton Down, Salisbury, Wilts UK SP4 0JQ, UK
Microb Pathog 42:11-21. 2007..Serum samples from representative macaques within this time period also inhibited the Yersinia-mediated cytotoxicity of J774 macrophages in a qualitative in vitro assay of type three secretion...
- Immunological aspects of polymer microsphere vaccine delivery systemsJ E Eyles
Biomedical Sciences, Dstl, Porton Downs, Salisbury, UK
J Drug Target 11:509-14. 2003..These data support the tenet that microencapsulation serves to modify the uptake, trafficking and processing of antigens...
- Vaccine development for potential bioterrorism agentsR W Titball
Defence Science and Technology Laboratory, Porton Down, Salisbury, UK
Curr Drug Targets Infect Disord 3:255-62. 2003..The prospects for the development of a new generation of bioterrorism vaccines which exploit these technologies are reviewed in this manuscript...
- PlagueE D Williamson
Defence Science and Technology Laboratory Dstl, Porton Down, Salisbury, Wilts SP4 0JQ, UK
Vaccine 27:D56-60. 2009..This paper reviews the progress towards an improved vaccine for plague and assesses the likely impact of a prophylactic vaccine for bubonic and pneumonic plague...
- Protection against plague following immunisation with microencapsulated V antigen is reduced by co-encapsulation with IFN-gamma or IL-4, but not IL-6K F Griffin
Dstl Biomedical Sciences, Porton Down, Salisbury, SP4 0JQ, Wiltshire, UK
Vaccine 20:3650-7. 2002..pestis strain GB; however protective efficacy was impaired by co-encapsulating either IFN-gamma or IL-4 with rV...
- Co-immunisation with a plasmid DNA cocktail primes mice against anthrax and plagueE D Williamson
Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 OJQ, UK
Vaccine 20:2933-41. 2002..pestis compared with priming only with plasmid DNA encoding the V antigen and boosting with rV. This enhancement may be due to the effect of CpG motifs known to be present in the plasmid DNA construct encoding PA...
- Stimulation of spleen cells in vitro by nanospheric particles containing antigenJ E Eyles
Dstl, Porton Down, SP4 0JQ, Salisbury, UK
J Control Release 86:25-32. 2003..This mechanism is likely to be distinct from non-specific effects caused by components of the delivery vehicle itself...
- The fraction 1 and V protein antigens of Yersinia pestis activate dendritic cells to induce primary T cell responsesR Kingston
Antigen Presentation Research Group, Imperial College London, Northwick Park and St Mark s Campus, Watford Road, Harrow, UK
Clin Exp Immunol 149:561-9. 2007..This study suggests an important role for DC, or factors secreted by them, in the induction of protective immunity to plague by the rF1 and rV antigens...
- An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in miceP C Oyston
Chemical and Biological Defence Establishment, Salisbury, Wiltshire, UK
Microbiology 142:1847-53. 1996..Unusually for an aro-defective mutant, the Y. pestis aroA mutant was virulent in mice, with a median dose which induced morbidity of death similar to that of the wild-type, although time to death was significantly prolonged...
- Tissue distribution of radioactivity following intranasal administration of radioactive microspheresJ E Eyles
Pharmaceutical Sciences, Life and Health Sciences, Aston University, Birmingham, UK
J Pharm Pharmacol 53:601-7. 2001..This effect may contribute to the effectiveness of pulmonary delivered antigen-loaded microparticles as humoral immunogens...
- Passive transfer of protection against Bacillus anthracis infection in a murine modelR J Beedham
Pathobiology, CBD, DERA Porton Down, Salisbury, SP4 0JQ, Wiltshire, UK
Vaccine 19:4409-16. 2001..The results demonstrated that an antibody response maybe important in protection against B. anthracis infection, under the conditions tested. The results provide further data for the development of an improved anthrax vaccine...
- Distribution of productive antigen-processing activity for MHC class II presentation in macrophagesA von Delwig
Musculoskeletal Research Group, Clinical Medical Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, UK
Scand J Immunol 62:243-50. 2005..The data suggest that endosomal compartments expressing Rab5 guanosine triphosphatase can productively process protein antigens for presentation by mature MHC class II molecules...
- Microsphere translocation and immunopotentiation in systemic tissues following intranasal administrationJ E Eyles
DERA (Chemical and Biological Defence Sector, Porton Down, Wiltshire SP4 OJQ, Salisbury, UK
Vaccine 19:4732-42. 2001....
- Molecular variation between the alpha-toxins from the type strain (NCTC 8237) and clinical isolates of Clostridium perfringens associated with disease in man and animalsA Ginter
Division Immunologie Animale, Centre d Economie Rurale, Marloie, Belgium
Microbiology 142:191-8. 1996..The changes in amino acid sequence did not affect the ability of a C-terminal domain vaccine, derived from the alpha-toxin of strain NCTC 8237, to induce protection against the alpha-toxin from a bovine enteric strain of C. perfringens...
- Macromolecular organization of the Yersinia pestis capsular F1 antigen: insights from time-of-flight mass spectrometryM A Tito
Oxford Centre for Molecular Sciences, New Chemistry Laboratory, Oxford, OX1 3QT, United Kingdom
Protein Sci 10:2408-13. 2001..More generally, the data show that the symmetry and packing of macromolecular complexes can be determined solely from mass spectrometry, without any prior knowledge of higher order structure..
- Probing molecular interactions in intact antibody: antigen complexes, an electrospray time-of-flight mass spectrometry approachM A Tito
Oxford Centre for Molecular Sciences, New Chemistry Laboratory, Oxford OX1 3QT, United Kingdom
Biophys J 81:3503-9. 2001..More generally this work demonstrates a rapid means of assessing antigen subunit interactions as well as the stoichiometry and specificity of binding in antibody-antigen complexes...
- Vaccines against dangerous pathogensE D Williamson
Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, UK
Br Med Bull 62:163-73. 2002..Emphasis is also placed on the derivation of surrogate markers of efficacy and a demonstration that these correlate with protection in the animal model...