S A Brooks

Summary

Affiliation: Oxford Brookes University
Country: UK

Publications

  1. ncbi request reprint Appropriate glycosylation of recombinant proteins for human use: implications of choice of expression system
    Susan A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford, OX3 0BP, UK
    Mol Biotechnol 28:241-55. 2004
  2. ncbi request reprint Protein glycosylation in diverse cell systems: implications for modification and analysis of recombinant proteins
    Susan A Brooks
    Oxford Brookes University, School of Biological and Molecular Sciences, Gipsy Lane, Headington, Oxford, OX3 0BP, UK
    Expert Rev Proteomics 3:345-59. 2006
  3. doi request reprint Lectin histochemistry to detect altered glycosylation in cells and tissues
    Susan A Brooks
    Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, Headington, UK
    Methods Mol Biol 878:31-50. 2012
  4. doi request reprint Basic immunocytochemistry for light microscopy
    Susan A Brooks
    Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, Headington, UK
    Methods Mol Biol 878:1-30. 2012
  5. doi request reprint Strategies for analysis of the glycosylation of proteins: current status and future perspectives
    Susan A Brooks
    School of Life Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford OX3 0BP, UK
    Mol Biotechnol 43:76-88. 2009
  6. doi request reprint Molecular interactions in cancer cell metastasis
    Susan A Brooks
    Oxford Brookes University, Headington, UK
    Acta Histochem 112:3-25. 2010
  7. ncbi request reprint Immunolocalisation of members of the polypeptide N-acetylgalactosaminyl transferase (ppGalNAc-T) family is consistent with biologically relevant altered cell surface glycosylation in breast cancer
    Susan A Brooks
    School of Life Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford OX3 0BP, UK
    Acta Histochem 109:273-84. 2007
  8. ncbi request reprint Expression of N-acetyl galactosaminylated and sialylated glycans by metastases arising from primary breast cancer
    S A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK
    Invasion Metastasis 18:115-21. 1998
  9. ncbi request reprint Investigations into the potential role of aberrant N-acetylgalactosamine glycans in tumour cell interactions with basement membrane components
    Susan A Brooks
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK
    Clin Exp Metastasis 19:487-93. 2002
  10. ncbi request reprint The involvement of Helix pomatia lectin (HPA) binding N-acetylgalactosamine glycans in cancer progression
    S A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK
    Histol Histopathol 15:143-58. 2000

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Appropriate glycosylation of recombinant proteins for human use: implications of choice of expression system
    Susan A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford, OX3 0BP, UK
    Mol Biotechnol 28:241-55. 2004
    ..This review gives a brief overview of human N- and O-linked protein glycosylation, summarizes what is known of the glycosylation potential of the cells of nonhuman species, and presents the implications for the biotechnology industry...
  2. ncbi request reprint Protein glycosylation in diverse cell systems: implications for modification and analysis of recombinant proteins
    Susan A Brooks
    Oxford Brookes University, School of Biological and Molecular Sciences, Gipsy Lane, Headington, Oxford, OX3 0BP, UK
    Expert Rev Proteomics 3:345-59. 2006
    ..The emergence of a new generation of expression systems with sophisticated engineering for humanized glycosylation of glycoproteins appears to be on the horizon...
  3. doi request reprint Lectin histochemistry to detect altered glycosylation in cells and tissues
    Susan A Brooks
    Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, Headington, UK
    Methods Mol Biol 878:31-50. 2012
    ..The use of both the enzyme label, horseradish peroxidase, and fluorescent labels is considered...
  4. doi request reprint Basic immunocytochemistry for light microscopy
    Susan A Brooks
    Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, Headington, UK
    Methods Mol Biol 878:1-30. 2012
    ..The use of enzyme labels including horseradish peroxidase and alkaline phosphatase, and fluorescent labels, are considered...
  5. doi request reprint Strategies for analysis of the glycosylation of proteins: current status and future perspectives
    Susan A Brooks
    School of Life Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford OX3 0BP, UK
    Mol Biotechnol 43:76-88. 2009
    ....
  6. doi request reprint Molecular interactions in cancer cell metastasis
    Susan A Brooks
    Oxford Brookes University, Headington, UK
    Acta Histochem 112:3-25. 2010
    ....
  7. ncbi request reprint Immunolocalisation of members of the polypeptide N-acetylgalactosaminyl transferase (ppGalNAc-T) family is consistent with biologically relevant altered cell surface glycosylation in breast cancer
    Susan A Brooks
    School of Life Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford OX3 0BP, UK
    Acta Histochem 109:273-84. 2007
    ....
  8. ncbi request reprint Expression of N-acetyl galactosaminylated and sialylated glycans by metastases arising from primary breast cancer
    S A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK
    Invasion Metastasis 18:115-21. 1998
    ..Binding patterns of alpha-GalNAc specific HPA and DBA, and a monoclonal antibody against Tn epitope (GalNAc-O-Ser/Thr) were markedly different...
  9. ncbi request reprint Investigations into the potential role of aberrant N-acetylgalactosamine glycans in tumour cell interactions with basement membrane components
    Susan A Brooks
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK
    Clin Exp Metastasis 19:487-93. 2002
    ..Research in our laboratory is ongoing to seek a functional role for the HPA-binding glycoconjugates in other aspects of the metastatic cascade...
  10. ncbi request reprint The involvement of Helix pomatia lectin (HPA) binding N-acetylgalactosamine glycans in cancer progression
    S A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK
    Histol Histopathol 15:143-58. 2000
    ....
  11. ncbi request reprint N-acetylgalactosamine, N-acetylglucosamine and sialic acid expression in primary breast cancers
    S A Brooks
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK
    Acta Histochem 103:37-51. 2001
    ....
  12. ncbi request reprint Validation of a simple avidin-biotin detection method for Helix pomatia lectin (HPA) binding as a prognostic marker in cancer
    Susan A Brooks
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Headington, Oxford, UK
    Acta Histochem 105:205-12. 2003
    ..It is hoped that its adoption may encourage research into this clinically significant alteration in cellular glycosylation...
  13. pmc GalNAc glycoprotein expression by breast cell lines, primary breast cancer and normal breast epithelial membrane
    S A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford, OX3 0BP, UK
    Br J Cancer 85:1014-22. 2001
    ..Moreover, very marked quantitative but not qualitative differences in levels of expression consistent with metastatic capability were noted...
  14. ncbi request reprint Altered glycosylation of proteins in cancer: what is the potential for new anti-tumour strategies
    S A Brooks
    School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford, OX3 0BP, UK
    Anticancer Agents Med Chem 8:2-21. 2008
    ..The significance that cancer-associated changes in glycoprotein glycosylation may have in new approaches to anti-tumour therapies is explored...
  15. ncbi request reprint Detection of aberrant glycosylation in breast cancer using lectin histochemistry
    Tracey M Carter
    School of Biological and Molecular Sciences, Oxford Brookes University, Headington, Oxford, UK
    Methods Mol Med 120:201-16. 2006
    ..The methods given here are broadly applicable and can be modified to investigate virtually any glycosylation change of potential interest in breast cancer research...
  16. ncbi request reprint In vivo xenograft models of breast cancer metastasis
    Ursula Valentiner
    Institute of Anatomy, University Hospital Eppendorf, Hamburg, Germany
    Methods Mol Med 120:479-88. 2006
    ..This model system was created to help to define the rate-limiting steps of the metastatic cascade...
  17. ncbi request reprint SDS-PAGE and Western blotting to detect proteins and glycoproteins of interest in breast cancer research
    Chloe Osborne
    Department of Physiology, University of Birmingham Medical School, UK
    Methods Mol Med 120:217-29. 2006
    ..In this chapter, the theory and principles of SDS-PAGE and Western blotting are briefly outlined, and basic methods are given that can be applied to investigate virtually any (glyco)protein of interest in breast cancer research...
  18. ncbi request reprint The lectin Helix pomatia agglutinin as a marker of metastases--clinical and experimental studies
    Udo Schumacher
    Institute for Anatomy II Experimental Morphology, University Hospital Hamburg Eppendorf, Martinistrasse 52, D 20246 Hamburg, Germany
    Anticancer Res 25:1829-30. 2005
    ..In conjunction with modern imaging techniques, these models will help to elucidate the molecular mechanisms governing the metastatic spread of tumours...
  19. ncbi request reprint HPA binding and metastasis formation of human breast cancer cell lines transplanted into severe combined immunodeficient (scid) mice
    Ursula Valentiner
    Institute for Anatomy II Experimental Morphology, University Hospital Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
    Cancer Lett 219:233-42. 2005
    ..The HPA binding glycoconjugates appear to be associated with adhesive interactions between metastasising tumour cells and endothelial cells...