Stephen Poole

Summary

Affiliation: National Institute for Biological Standards and Control
Country: UK

Publications

  1. ncbi request reprint Streptococcus sanguis secretes CD14-binding proteins that stimulate cytokine synthesis: a clue to the pathogenesis of infective (bacterial) endocarditis?
    Julia Banks
    Cellular Microbiology Research Group, Eastman Dental Institute, University College London, 256 Gray s Inn Road, London, Hertfordshire, WC1X 8LD, U K
    Microb Pathog 32:105-16. 2002
  2. ncbi request reprint A rapid 'one-plate' in vitro test for pyrogens
    Stephen Poole
    National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, England, UK
    J Immunol Methods 274:209-20. 2003
  3. doi request reprint Comparison of novel methods for predicting the risk of pro-inflammatory clinical infusion reactions during monoclonal antibody therapy
    Lucy Findlay
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    J Immunol Methods 371:134-42. 2011
  4. doi request reprint Antibody C region influences TGN1412-like functional activity in vitro
    Christina Ball
    National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, United Kingdom
    J Immunol 189:5831-40. 2012
  5. pmc Severity of the TGN1412 trial disaster cytokine storm correlated with IL-2 release
    David Eastwood
    Biotherapeutics Group, NIBSC, Potters Bar, Hertfordshire, UK
    Br J Clin Pharmacol 76:299-315. 2013
  6. doi request reprint Improved in vitro methods to predict the in vivo toxicity in man of therapeutic monoclonal antibodies including TGN1412
    Lucy Findlay
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    J Immunol Methods 352:1-12. 2010
  7. doi request reprint Safety of biologics, lessons learnt from TGN1412
    Richard Stebbings
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    Curr Opin Biotechnol 20:673-7. 2009
  8. doi request reprint Endothelial cells co-stimulate peripheral blood mononuclear cell responses to monoclonal antibody TGN1412 in culture
    Lucy Findlay
    Biotherapeutics Group, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    Cytokine 55:141-51. 2011
  9. ncbi request reprint Monocyte activation test for pro-inflammatory and pyrogenic contaminants of parenteral drugs: test design and data analysis
    Rose E Gaines Das
    National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, UK
    J Immunol Methods 288:165-77. 2004
  10. ncbi request reprint "Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics
    Richard Stebbings
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, United Kingdom
    J Immunol 179:3325-31. 2007

Collaborators

  • Richard Stebbings
  • Robin Thorpe
  • Meenu Wadhwa
  • C Jane Robinson
  • Mariana L Vale
  • Lois M Harden
  • Annemarie Ledeboer
  • V H Perry
  • S J Thorpe
  • Marcelo H Napimoga
  • Lucy Findlay
  • Thiago M Cunha
  • David Eastwood
  • Waldiceu A Verri
  • Christina Ball
  • Sergio H Ferreira
  • Fernando Q Cunha
  • Giamal N Luheshi
  • Erin D Milligan
  • Danielle G Souza
  • Carlos A Parada
  • Yogesh Mistry
  • Giles Sharp
  • Mauro M Teixeira
  • Steven F Maier
  • Linda R Watkins
  • David Martin
  • Marucia Chacur
  • Christelle Sachot
  • Brian Henderson
  • Chris Bird
  • Bernard Fox
  • Wataru Inoue
  • Adrian Bristow
  • Jo C Lewthwaite
  • Diana K Schoeniger-Skinner
  • Teresa Mateo
  • Argel Aguilar-Valles
  • Anthony R M Coates
  • Christoph Rummel
  • Sebastian Hoffmann
  • Maria Maguire
  • Fabian Docagne
  • Peter Brugger
  • Marie Eve Fortier
  • Carin M Twining
  • Rose E Gaines Das
  • Vanessa Pinho
  • Julia Banks
  • Daniela Sachs
  • Berenice B Lorenzetti
  • Tarek El-Haj
  • Otilia Obreja
  • Paula Dilger
  • Jason Hockley
  • Simon Hufton
  • Paul W H I Parren
  • Christopher Bird
  • John Hood
  • Silvio M Vieira
  • Gokce Somay
  • Ana T Guerrero
  • Daniel A Valerio
  • Ieda R Schivo
  • Luciana G Nogueira
  • Mercedes Losada
  • Rachel A Lawrence
  • Esteban J Morcillo
  • Matthew G Frank
  • Julio Cortijo
  • Ana T G Guerrero
  • Mahavir Singh
  • Caroline P D Wheeler-Jones
  • Rossana Estellés
  • Sandra Y Fukada
  • Yafa Naim Abu Nabah
  • Jose Miguel Cerda-Nicolas
  • Peter J Jose
  • Andrew A Pitsillides
  • Tania Santodomingo-Garzon
  • Claire E Clarkin
  • Begoña Bedrina
  • Maria Jesus Sanz
  • Sylvain Williams
  • Chantal Company
  • Peter Tormay
  • Jan de Lange
  • Sandra J Campbell
  • Carmen Guaza
  • Bente Mogster

Detail Information

Publications40

  1. ncbi request reprint Streptococcus sanguis secretes CD14-binding proteins that stimulate cytokine synthesis: a clue to the pathogenesis of infective (bacterial) endocarditis?
    Julia Banks
    Cellular Microbiology Research Group, Eastman Dental Institute, University College London, 256 Gray s Inn Road, London, Hertfordshire, WC1X 8LD, U K
    Microb Pathog 32:105-16. 2002
    ..In addition, blocking of CD14 may have some therapeutic benefit...
  2. ncbi request reprint A rapid 'one-plate' in vitro test for pyrogens
    Stephen Poole
    National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, England, UK
    J Immunol Methods 274:209-20. 2003
    ..The preferred readout is IL-6, which gives a limit of detection of 0.015 endotoxin units (EU)/ml with peripheral blood mononuclear cell (PBMNC), 0.03 EU/ml with diluted whole blood and 0.05 EU/ml with a monocytic cell line (MONO MAC 6)...
  3. doi request reprint Comparison of novel methods for predicting the risk of pro-inflammatory clinical infusion reactions during monoclonal antibody therapy
    Lucy Findlay
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    J Immunol Methods 371:134-42. 2011
    ..Overall, the antibodies tested could be ranked as follows: Tysabri<Avastin<Herceptin<Campath-1H<TGN1412, with only the "superagonistic" CD28 monoclonal antibody (TGN1412) stimulating IL-2 release and cell proliferation...
  4. doi request reprint Antibody C region influences TGN1412-like functional activity in vitro
    Christina Ball
    National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, United Kingdom
    J Immunol 189:5831-40. 2012
    ..We propose that the Fc, potentially through restricting flexibility, maintains a favorable V region conformation to allow superagonistic activity. These findings have important implications for Ab design strategies...
  5. pmc Severity of the TGN1412 trial disaster cytokine storm correlated with IL-2 release
    David Eastwood
    Biotherapeutics Group, NIBSC, Potters Bar, Hertfordshire, UK
    Br J Clin Pharmacol 76:299-315. 2013
    ..To determine if cytokine release with a solid phase assay is predictive of adverse responses for a range of therapeutic mAbs...
  6. doi request reprint Improved in vitro methods to predict the in vivo toxicity in man of therapeutic monoclonal antibodies including TGN1412
    Lucy Findlay
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    J Immunol Methods 352:1-12. 2010
    ..It was concluded that immobilising mAbs by air-drying offers a useful in vitro method for detecting and quantifying pro-inflammatory activities of therapeutic mAbs...
  7. doi request reprint Safety of biologics, lessons learnt from TGN1412
    Richard Stebbings
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    Curr Opin Biotechnol 20:673-7. 2009
    ..Perhaps the most important lesson is that greater caution needs to be exercised when evaluating new biologics...
  8. doi request reprint Endothelial cells co-stimulate peripheral blood mononuclear cell responses to monoclonal antibody TGN1412 in culture
    Lucy Findlay
    Biotherapeutics Group, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    Cytokine 55:141-51. 2011
    ....
  9. ncbi request reprint Monocyte activation test for pro-inflammatory and pyrogenic contaminants of parenteral drugs: test design and data analysis
    Rose E Gaines Das
    National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, UK
    J Immunol Methods 288:165-77. 2004
    ..Products tested include small molecules, biologicals and vaccines. The PBMNC/IL-6 monocyte activation test has been approved by the US FDA as an 'end-product release test' and also is being used for in-process testing...
  10. ncbi request reprint "Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics
    Richard Stebbings
    Biotherapeutics Group, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, United Kingdom
    J Immunol 179:3325-31. 2007
    ....
  11. ncbi request reprint Circulating interleukin-6 induces fever through a STAT3-linked activation of COX-2 in the brain
    Christoph Rummel
    Douglas Hospital Research Centre, Dept of Psychiatry, McGill Univ, 6875 Blvd LaSalle, Verdun, Montreal, QC, H4H 1R3, Canada
    Am J Physiol Regul Integr Comp Physiol 291:R1316-26. 2006
    ..These observations suggest that IL-6 is an important circulating pyrogen that activates the COX-2-pathway in cerebral microvasculature, most likely through a STAT3-dependent pathway...
  12. ncbi request reprint Interleukin-6 and leptin mediate lipopolysaccharide-induced fever and sickness behavior
    Lois M Harden
    School of Physiology, Medical School, Brain Function Research Unit, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, South Africa
    Physiol Behav 89:146-55. 2006
    ..05). Injection of anti-TNF-alpha or anti-IL-1beta serum had no effect on LPS-induced sickness behavior. Peripherally released IL-6 and leptin therefore appear to be important in regulating LPS-induced fever and sickness behavior...
  13. ncbi request reprint The viral mimic, polyinosinic:polycytidylic acid, induces fever in rats via an interleukin-1-dependent mechanism
    Marie Eve Fortier
    Douglas Hospital Research Centre, McGill Univ, 6875 LaSalle Boulevard, Verdun, Quebec, Canada
    Am J Physiol Regul Integr Comp Physiol 287:R759-66. 2004
    ..Finally, poly I:C decreased food intake by 30%, but this response was not altered by pretreatment with IL-1ra. These results suggest that poly I:C induces fever, but not anorexia, through an IL-1 and prostaglandin-dependent mechanism...
  14. ncbi request reprint Highly homologous Mycobacterium tuberculosis chaperonin 60 proteins with differential CD14 dependencies stimulate cytokine production by human monocytes through cooperative activation of p38 and ERK1/2 mitogen-activated protein kinases
    Jo C Lewthwaite
    Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, UK
    Int Immunopharmacol 7:230-40. 2007
    ....
  15. pmc Interleukin-6 mediates low-threshold mechanical allodynia induced by intrathecal HIV-1 envelope glycoprotein gp120
    Diana K Schoeniger-Skinner
    Department of Psychology and the Center for Neuroscience, University of Colorado at Boulder, Boulder, CO 30809 0345, USA
    Brain Behav Immun 21:660-7. 2007
    ..These results would suggest that IL-6 induces pain facilitation, and may do so in part by stimulating the production and release of other pro-inflammatory cytokines...
  16. pmc Attenuated fever in rats during late pregnancy is linked to suppressed interleukin-6 production after localized inflammation with turpentine
    Argel Aguilar-Valles
    Douglas Hospital Research Centre, Department of Psychiatry, McGill University, 6875 LaSalle Boulevard, Verdun, Quebec, Canada H4H 1R3
    J Physiol 583:391-403. 2007
    ..These data further confirm the importance of IL-6 in fever generation and provide evidence that it may be a key component of the attenuated fever response in late pregnancy...
  17. ncbi request reprint A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles
    Teresa Mateo
    Department of Pharmacology, Faculty of Medicine, University of Valencia, Spain
    Blood 110:1895-902. 2007
    ..Therefore, neutralization of TNFalpha activity may help to prevent mononuclear cell infiltration and the progression of the atherogenic process...
  18. ncbi request reprint TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor
    Thiago M Cunha
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto University of Sao Paulo, Av Bandeirantes, 3900, 14049 900, Ribeirao Preto, SP, Brazil
    Eur J Pharmacol 573:221-9. 2007
    ..On the other hand, in LPS-primed mice, the B(1) kinin receptor mediates hypernociception by a mechanism dependent on TNF-alpha and IL-1beta, which could stimulate prostanoid and sympathetic amine production...
  19. doi request reprint Crucial role of neutrophils in the development of mechanical inflammatory hypernociception
    Thiago M Cunha
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
    J Leukoc Biol 83:824-32. 2008
    ..Therefore, the blockade of neutrophil migration could be a target to development of new analgesic drugs...
  20. doi request reprint Interleukin (IL)-6 and IL-1 beta act synergistically within the brain to induce sickness behavior and fever in rats
    Lois M Harden
    Brain Function Research Group, School of Physiology, Medical School, University of the Witwatersrand, 7 York Road, Parktown, Gauteng, Johannesburg, South Africa
    Brain Behav Immun 22:838-49. 2008
    ..These results show that species-homologous rat IL-6 and IL-1 beta can act directly within the brain to decrease voluntary activity and suggest they also can act synergistically to induce anorexia and fever...
  21. doi request reprint Role of cytokines in mediating mechanical hypernociception in a model of delayed-type hypersensitivity in mice
    Thiago M Cunha
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto University of Sao Paulo, Av Bandeirantes, 3900, 14049 900 Ribeirao Preto, SP, Brazil
    Eur J Pain 12:1059-68. 2008
    ..The latter cytokines in turn stimulate the release of the direct-acting final mediators, prostanoids and sympathetic amines...
  22. pmc Immune-to-brain signaling and central prostaglandin E2 synthesis in fasted rats with altered lipopolysaccharide-induced fever
    Wataru Inoue
    Douglas Mental Health Univ Institute, Department of Psychiatry, McGill University, Montreal, Quebec, Canada
    Am J Physiol Regul Integr Comp Physiol 295:R133-43. 2008
    ....
  23. ncbi request reprint Hypernociceptive role of cytokines and chemokines: targets for analgesic drug development?
    Waldiceu A Verri
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto University of Sao Paulo, Av Bandeirantes, 3900, 14049 900, Ribeirao Preto, SP, Brazil
    Pharmacol Ther 112:116-38. 2006
    ....
  24. ncbi request reprint Interleukin-6 in combination with its soluble IL-6 receptor sensitises rat skin nociceptors to heat, in vivo
    Otilia Obreja
    Institut fur Physiologie und Experimentelle Pathophysiologie, Universitatsstrasse 17, Erlangen, Germany
    Pain 96:57-62. 2002
    ....
  25. ncbi request reprint Tumour necrosis factor-alpha, interleukin-1beta and interleukin-8 induce persistent mechanical nociceptor hypersensitivity
    Daniela Sachs
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
    Pain 96:89-97. 2002
    ..However, TNF-alpha-induced mechanical nociceptor hypersensitivity results from the concomitant endogenous release of eicosanoids and sympathomimetic mediators...
  26. ncbi request reprint Effect of a BLT receptor antagonist in a model of severe ischemia and reperfusion injury in the rat
    Danielle G Souza
    Laboratorio de Imunofarmacologia, Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627 Pampulha, 31270 901 Belo Horizonte, MG, Brazil
    Eur J Pharmacol 440:61-9. 2002
    ..Overall, our results show that activation of BLT receptor plays a minor role in the local, remote and systemic injuries following severe ischemia and reperfusion in rats...
  27. ncbi request reprint Antinociceptive effects of interleukin-4, -10, and -13 on the writhing response in mice and zymosan-induced knee joint incapacitation in rats
    Mariana L Vale
    Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil
    J Pharmacol Exp Ther 304:102-8. 2003
    ..This analgesic effect could be related to a peripheral mechanism, probably via the inhibition of the release of the pro-inflammatory cytokines TNF-alpha and IL-1beta by resident peritoneal macrophages...
  28. ncbi request reprint Cytokine-induced neutrophil chemoattractant 1 (CINC-1) mediates the sympathetic component of inflammatory mechanical hypersensitivitiy in rats
    Berenice B Lorenzetti
    Department of Pharmacology, Institute of Biological Science, Universidade Federal do Parana, Brazil
    Eur Cytokine Netw 13:456-61. 2002
    ..These data suggest that CINC-1, released at sites of inflammation, mediates inflammatory hyperalgesia in rats via release of sympathomimetic amines...
  29. ncbi request reprint Spinal glia and proinflammatory cytokines mediate mirror-image neuropathic pain in rats
    Erin D Milligan
    Department of Psychology and the Center for Neuroscience, University of Colorado at Boulder, Boulder, Colorado 80309 0345, USA
    J Neurosci 23:1026-40. 2003
    ..These results provide the first evidence that ipsilateral and mirror-image inflammatory neuropathy pain are created both acutely and chronically through glial and proinflammatory cytokine actions...
  30. ncbi request reprint IL-1-driven endogenous IL-10 production protects against the systemic and local acute inflammatory response following intestinal reperfusion injury
    Danielle G Souza
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    J Immunol 170:4759-66. 2003
    ..Thus, IL-1 plays a major role in driving endogenous IL-10 production and protects against the TNF-alpha-dependent systemic and local acute inflammatory response following intestinal reperfusion injury...
  31. ncbi request reprint Snake venom phospholipase A2s (Asp49 and Lys49) induce mechanical allodynia upon peri-sciatic administration: involvement of spinal cord glia, proinflammatory cytokines and nitric oxide
    Marucia Chacur
    Instituto Butantan, Sao Paolo, Brazil
    Pain 108:180-91. 2004
    ..As a variety of immune cells also produce and release sPLA2s during inflammatory states, the data may have general implications for the understanding of inflammatory pain...
  32. ncbi request reprint Peri-sciatic proinflammatory cytokines, reactive oxygen species, and complement induce mirror-image neuropathic pain in rats
    Carin M Twining
    Department of Psychology and the Center for Neuroscience, University of Colorado at Boulder, Campus Box 345 Boulder, CO 80309 0345, USA
    Pain 110:299-309. 2004
    ..Thus these immune-derived substances can markedly alter sensory nerve function at mid-axon...
  33. pmc Circulating leptin mediates lipopolysaccharide-induced anorexia and fever in rats
    Christelle Sachot
    Douglas Hospital Research Center, McGill University, 6875 Boulevard LaSalle, Verdun, Quebec H4H 1R3, Canada
    J Physiol 561:263-72. 2004
    ..These results are the first to demonstrate that leptin is a circulating mediator of LPS-induced anorexia and fever, probably through a hypothalamic IL-1beta-dependent mechanism...
  34. pmc Antihyperalgesic effect of pentoxifylline on experimental inflammatory pain
    Mariana L Vale
    Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceara, Rua Cel, Nunes de Melo, 1127, CEP 60430 270, Fortaleza, CE, Brazil
    Br J Pharmacol 143:833-44. 2004
    ..In conclusion, the antinociceptive activity of pentoxifylline is associated with the inhibition of the release of both TNF-alpha and IL-1beta...
  35. ncbi request reprint Differential regulation of type I and type II interleukin-1 receptors in focal brain inflammation
    Fabian Docagne
    Molecular Neuropathology Laboratory, School of Biological Sciences, University of Southampton, UK
    Eur J Neurosci 21:1205-14. 2005
    ..The lesion-specific expression of IL-1 receptors suggests that the receptors are differentially regulated in a manner not directly related to the endogenous level of IL-1 in the CNS...
  36. ncbi request reprint International validation of novel pyrogen tests based on human monocytoid cells
    Sebastian Hoffmann
    Institute of Biochemical Pharmacology and Steinbeis Center InPuT, University of Konstanz, Universitatsstrasse 10, D 78457 Konstanz, Germany
    J Immunol Methods 298:161-73. 2005
    ..Our data suggest that animal testing could be completely replaced by these evidence-based pyrogen tests and highlight their potential to further improve drug safety...
  37. pmc Comparative cell signalling activity of ultrapure recombinant chaperonin 60 proteins from prokaryotes and eukaryotes
    Maria Maguire
    Division of Microbial Diseases, Eastman Dental Institute, University College London, London, UK
    Immunology 115:231-8. 2005
    ....
  38. ncbi request reprint Nociceptive effect of subcutaneously injected interleukin-12 is mediated by endothelin (ET) acting on ETB receptors in rats
    Waldiceu A Verri
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil
    J Pharmacol Exp Ther 315:609-15. 2005
    ..Therefore, endothelin receptor antagonism could be beneficial in controlling IL-12 therapy-induced pain or hyperalgesia...
  39. ncbi request reprint Anti-nociceptive effect of thalidomide on zymosan-induced experimental articular incapacitation
    Mariana L Vale
    Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará R Cel Nunes de Melo 1127, Rodolfo Teofilo, 60 430 270 Fortaleza CE, Brazil
    Eur J Pharmacol 536:309-17. 2006
    ..In conclusion, the anti-nociceptive activity of thalidomide in zymosan-induced articular incapacitation is associated with the inhibition of TNF-alpha by resident synovial cells...
  40. ncbi request reprint Anorexia in a rat model of colitis: interaction of interleukin-1 and hypothalamic serotonin
    Tarek El-Haj
    Department of Adult and Paediatric Gastroenterology, St Bartholomew s and the Royal London School of Medicine and Dentistry, 2 Newark Street, London E1 2AT, UK
    Brain Res 927:1-7. 2002
    ..However, a functional link between central IL-1 receptors and 5-HT release remains unproven. We have tested the hypothesis that anorexia associated with experimental colitis is mediated by IL-1 induced release of 5-HT...