Michael J Gilchrist
Affiliation: National Institute for Medical Research
- Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencingFlorencia del Viso
Department of Pediatrics and Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
BMC Genomics 13:649. 2012..To facilitate cloning of X. tropicalis mutants identified in forward genetic screens, we sought to combine bulk segregant analysis and exome sequencing into a single step...
- Databases of gene expression in Xenopus developmentMichael J Gilchrist
Division of Systems Biology, MRC National Institute for Medical Research, London, UK
Methods Mol Biol 917:319-45. 2012..Instructions are given on how to access and interpret the data provided by the NCBI Gene database, Xenbase, and the Xenopus full-length EST, quickImage, and Xenmark databases...
- From expression cloning to gene modeling: the development of Xenopus gene sequence resourcesMichael J Gilchrist
Division of Systems Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom
Genesis 50:143-54. 2012..In addition, I demonstrate that the gaps in the current genome assembly create problems for the computational elucidation of gene sequences, and suggest some ways to ameliorate the effects of this...
- A study of alternative splicing in the pigAnn Britt Nygard
University of Copenhagen, Faculty of Life Sciences, Department of Basic Animal and Veterinary Sciences, Division of Genetics and Bioinformatics, Groennegaardsvej 3, 1870 Frederiksberg C, Denmark
BMC Res Notes 3:123. 2010..With the EST resource for the domestic pig now containing more than one million porcine ESTs, it is possible to identify alternative splice forms of the individual transcripts in this species from the EST data with some confidence...
- Genome-wide analysis of gene expression during Xenopus tropicalis tadpole tail regenerationNick R Love
Faculty of Life Sciences, University of Manchester, UK
BMC Dev Biol 11:70. 2011..Here, we explore tadpole tail regeneration in Xenopus tropicalis, a diploid frog with a sequenced genome...
- Molecular asymmetry in the 8-cell stage Xenopus tropicalis embryo described by single blastomere transcript sequencingElena De Domenico
The Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, Mill Hill, London NW7 1AA, UK
Dev Biol 408:252-68. 2015..Mutations in the human ortholog of the most animally enriched novel gene, Slc35d1, are causative for Schneckenbecken dysplasia, and we show that a similar phenotype is produced by depletion of the orthologous protein in Xenopus embryos. ..
- High-resolution analysis of gene activity during the Xenopus mid-blastula transitionClara Collart
Division of Systems Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
Development 141:1927-39. 2014..Together, our high-resolution time series and experimental studies have yielded a deeper understanding of the temporal organisation of gene regulatory networks in the early Xenopus embryo. ..