B W Wren

Summary

Affiliation: London School of Hygiene and Tropical Medicine
Country: UK

Publications

  1. pmc Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis
    Stewart J Hinchliffe
    The London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    Genome Res 13:2018-29. 2003
  2. pmc Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations
    Gareth A Maglennon
    Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, AL9 7TA, UK
    Vet Res 44:124. 2013
  3. pmc Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae
    Gareth A Maglennon
    Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield AL9 7TA, United Kingdom
    Vet Res 44:63. 2013
  4. pmc Clostridium difficile has a single sortase, SrtB, that can be inhibited by small-molecule inhibitors
    Elizabeth H Donahue
    Pathogen Molecular Biology Department, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    BMC Microbiol 14:219. 2014
  5. pmc The agr locus regulates virulence and colonization genes in Clostridium difficile 027
    Melissa J Martin
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, University of London, London, United Kingdom
    J Bacteriol 195:3672-81. 2013
  6. pmc Macro and micro diversity of Clostridium difficile isolates from diverse sources and geographical locations
    Richard A Stabler
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS ONE 7:e31559. 2012
  7. pmc Exploitation of bacterial N-linked glycosylation to develop a novel recombinant glycoconjugate vaccine against Francisella tularensis
    Jon Cuccui
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Open Biol 3:130002. 2013
  8. pmc Characterisation of Clostridium difficile biofilm formation, a role for Spo0A
    Lisa F Dawson
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS ONE 7:e50527. 2012
  9. pmc Hypervirulent Clostridium difficile PCR-ribotypes exhibit resistance to widely used disinfectants
    Lisa F Dawson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS ONE 6:e25754. 2011
  10. pmc Comparative phylogenomics of Streptococcus pneumoniae isolated from invasive disease and nasopharyngeal carriage from West Africans
    Eric S Donkor
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, UK
    BMC Genomics 13:569. 2012

Detail Information

Publications96

  1. pmc Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis
    Stewart J Hinchliffe
    The London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    Genome Res 13:2018-29. 2003
    ..pseudotuberculosis strains. This study reports chromosomal differences between species, biovars, serotypes, and strains of Y. pestis and Y. pseudotuberculosis that may relate to the evolution of these species in their respective niches...
  2. pmc Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations
    Gareth A Maglennon
    Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, AL9 7TA, UK
    Vet Res 44:124. 2013
    ..The ability to generate random mutant libraries is a powerful tool in the further study of the pathogenesis of this important swine pathogen...
  3. pmc Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae
    Gareth A Maglennon
    Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield AL9 7TA, United Kingdom
    Vet Res 44:63. 2013
    ..In conclusion, we have generated a set of plasmids that will be valuable in studies of M. hyopneumoniae pathogenesis and provide a major step forward in the study of this important swine pathogen. ..
  4. pmc Clostridium difficile has a single sortase, SrtB, that can be inhibited by small-molecule inhibitors
    Elizabeth H Donahue
    Pathogen Molecular Biology Department, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    BMC Microbiol 14:219. 2014
    ....
  5. pmc The agr locus regulates virulence and colonization genes in Clostridium difficile 027
    Melissa J Martin
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, University of London, London, United Kingdom
    J Bacteriol 195:3672-81. 2013
    ..Thus, the agr locus likely plays a contributory role in the fitness and virulence potential of C. difficile strains in the 027/BI/NAP1 lineage. ..
  6. pmc Macro and micro diversity of Clostridium difficile isolates from diverse sources and geographical locations
    Richard A Stabler
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS ONE 7:e31559. 2012
    ..This study helps to define the evolutionary origin of dominant C. difficile lineages and demonstrates that C. difficile is continuing to evolve in concert with human activity...
  7. pmc Exploitation of bacterial N-linked glycosylation to develop a novel recombinant glycoconjugate vaccine against Francisella tularensis
    Jon Cuccui
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Open Biol 3:130002. 2013
    ..tularensis subsp. holarctica. PGCT allows different polysaccharide and protein combinations to be produced recombinantly and could be easily applicable for the production of diverse glycoconjugate vaccines...
  8. pmc Characterisation of Clostridium difficile biofilm formation, a role for Spo0A
    Lisa F Dawson
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS ONE 7:e50527. 2012
    ..Our findings highlight an important attribute of C. difficile pathogenesis, which may have significant implications for infection, treatment and relapse...
  9. pmc Hypervirulent Clostridium difficile PCR-ribotypes exhibit resistance to widely used disinfectants
    Lisa F Dawson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS ONE 6:e25754. 2011
    ..difficile spores in healthcare settings...
  10. pmc Comparative phylogenomics of Streptococcus pneumoniae isolated from invasive disease and nasopharyngeal carriage from West Africans
    Eric S Donkor
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, UK
    BMC Genomics 13:569. 2012
    ..pneumoniae serotypes (1, 3, 5, 6A, 11, 14, 19 F and 23 F) of varying invasive disease potential...
  11. pmc Evidence of Yersinia pestis DNA from fleas in an endemic plague area of Zambia
    Bernard M Hang'ombe
    School of Veterinary Medicine, University of Zambia, Lusaka, Zambia
    BMC Res Notes 5:72. 2012
    ..In this study, we undertook plague surveillance by using PCR amplification of the plasminogen activator gene in fleas...
  12. pmc Bacterial epidemiology and biology--lessons from genome sequencing
    Julian Parkhill
    The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 12:230. 2011
    ..Next-generation sequencing has ushered in a new era of microbial genomics, enabling the detailed historical and geographical tracing of bacteria. This is helping to shape our understanding of bacterial evolution...
  13. pmc New levels of sophistication in the transcriptional landscape of bacteria
    Arnoud Hm van Vliet
    Institute of Food Research, Norwich NR4 7UA, UK
    Genome Biol 10:233. 2009
    ..An extra layer of complexity in the regulation of gene expression in bacteria is now apparent through previously unanticipated roles of noncoding and antisense RNAs...
  14. pmc Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium
    Richard A Stabler
    London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Genome Biol 10:R102. 2009
    ..difficile (CD196), a recent epidemic and hypervirulent 027 (R20291) and a previously sequenced PCR-ribotype 012 strain (630)...
  15. pmc Liposomal delivery of p-ialB and p-omp25 DNA vaccines improves immunogenicity but fails to provide full protection against B. melitensis challenge
    Nicola J Commander
    Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK
    Genet Vaccines Ther 8:5. 2010
    ..The present study was undertaken to further understand the immune response generated by the protective vaccination regimens and to evaluate cationic liposome adsorption as a delivery method to improve vaccine utility...
  16. pmc Identification and characterisation of a novel adhesin Ifp in Yersinia pseudotuberculosis
    Philippa C R Strong
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    BMC Microbiol 11:85. 2011
    ....
  17. pmc The analysis of para-cresol production and tolerance in Clostridium difficile 027 and 012 strains
    Lisa F Dawson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    BMC Microbiol 11:86. 2011
    ..It has been proposed that the hpdBCA operon, rarely found in other gut microflora, encodes the enzymes responsible for the conversion of p-HPA to p-cresol...
  18. pmc Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux
    Richard A Stabler
    Department of Infectious and Tropical Diseases, Keppel Street, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    BMC Microbiol 8:177. 2008
    ..We describe the construction and development of an Active Surveillance of Pathogens (ASP) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes...
  19. pmc Global transcriptional profiling of Burkholderia pseudomallei under salt stress reveals differential effects on the Bsa type III secretion system
    Pornpan Pumirat
    Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    BMC Microbiol 10:171. 2010
    ..pseudomallei. We therefore investigated the global transcriptional response of B. pseudomallei to increased salinity using microarray analysis...
  20. pmc Re-annotation and re-analysis of the Campylobacter jejuni NCTC11168 genome sequence
    Ozan Gundogdu
    Pathogen Molecular Department, London School of Hygiene and Tropical Medicine, UK
    BMC Genomics 8:162. 2007
    ..We now describe the complete re-annotation and re-analysis of the C. jejuni NCTC11168 genome using current database information, novel tools and annotation techniques not used during the original annotation...
  21. pmc Simple sequence repeats in Helicobacter canadensis and their role in phase variable expression and C-terminal sequence switching
    Lori A S Snyder
    Centre for Systems Biology, School of Biosciences, University of Birmingham, Edgbaston, Birmingham B152TT, UK
    BMC Genomics 11:67. 2010
    ..Helicobacter canadensis is an emerging human pathogen and zoonotic agent. The genome of H. canadensis was sequenced previously and determined to contain 29 annotated coding regions associated with homopolymeric tracts...
  22. ncbi request reprint Microbial genome analysis: insights into virulence, host adaptation and evolution
    B W Wren
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    Nat Rev Genet 1:30-9. 2000
    ..This wealth of information will catalyse the development of new intervention strategies to reduce the burden of microbial-related disease...
  23. ncbi request reprint The yersiniae--a model genus to study the rapid evolution of bacterial pathogens
    Brendan W Wren
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Nat Rev Microbiol 1:55-64. 2003
    ....
  24. ncbi request reprint Innate immunity: past, present and future
    B W Wren
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Mol Immunol 42:925-6. 2005
  25. ncbi request reprint Investigation into the role of the response regulator NtrC in the metabolism and virulence of Brucella suis
    N Dorrell
    Department of Medical Microbiology, St Bartholomew s and the Royal London School of Medicine and Dentistry, West Smithfield, London, UK
    Microb Pathog 27:1-11. 1999
    ..The mutation did not affect survival or multiplication of B. suis in macrophages, but during the initial stages of infection in the murine brucellosis model, the ntrC mutant showed a reduced ability to multiply rapidly in splenic tissue...
  26. ncbi request reprint Genome sequence of Yersinia pestis, the causative agent of plague
    J Parkhill
    The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 413:523-7. 2001
    ..The evidence of ongoing genome fluidity, expansion and decay suggests Y. pestis is a pathogen that has undergone large-scale genetic flux and provides a unique insight into the ways in which new and highly virulent pathogens evolve...
  27. pmc Mutational analysis of genes encoding the early flagellar components of Helicobacter pylori: evidence for transcriptional regulation of flagellin A biosynthesis
    E Allan
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom
    J Bacteriol 182:5274-7. 2000
    ..This is the first demonstration of a checkpoint in H. pylori flagellar assembly...
  28. pmc Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity
    N Dorrell
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Genome Res 11:1706-15. 2001
    ..These studies reveal extensive genetic diversity among C. jejuni strains and pave the way toward identifying correlates of pathogenicity and developing improved epidemiological tools for this problematic pathogen...
  29. pmc Detection and initial characterization of novel capsular polysaccharide among diverse Campylobacter jejuni strains using alcian blue dye
    A V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, London WC1E 7HT, United Kingdom
    J Clin Microbiol 39:279-84. 2001
    ..The identification of a newly described CPS and its lipid-free form in C. jejuni should prove invaluable in studying the pathogenesis and epidemiology of this important pathogen...
  30. ncbi request reprint Deciphering Campylobacter jejuni cell surface interactions from the genome sequence
    D Linton
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, WC1E 7HT, London, UK
    Curr Opin Microbiol 4:35-40. 2001
    ..jejuni, a C. jejuni protein that is translocated into eukaryotic cells, and plasmid-encoded components of a putative type IV secretion system are likely to be significant in terms of the host-pathogen interaction...
  31. ncbi request reprint Construction and characterisation of a Yersinia enterocolitica O:8 ompR mutant
    N Dorrell
    Department of Medical Microbiology, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    FEMS Microbiol Lett 165:145-51. 1998
    ..The mutant was shown to have increased sensitivity to high osmolarity, high temperature and low pH stresses in vitro. In the murine yersiniosis model, the mutant was attenuated and offered partial protection against wild-type challenge...
  32. ncbi request reprint A Caenorhabditis elegans model of Yersinia infection: biofilm formation on a biotic surface
    G W P Joshua
    London School of Hygiene and Tropical Medicine, Dept Infectious and Tropical Diseases, Keppel St, London WC1E 7HT, UK
    Microbiology 149:3221-9. 2003
    ..This suggests that biofilm formation on a biotic surface is an interactive process involving both bacterial and invertebrate control mechanisms...
  33. ncbi request reprint Characterization of the low-pH responses of Helicobacter pylori using genomic DNA arrays
    E Allan
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Microbiology 147:2285-92. 2001
    ..Genes down-regulated at pH 4.0 include those encoding a sugar nucleotide biosynthesis protein, a flagellar protein and an outer-membrane protein. Differential gene expression was confirmed by total RNA slot-blot hybridization...
  34. pmc Comparative phylogenomics of Clostridium difficile reveals clade specificity and microevolution of hypervirulent strains
    R A Stabler
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    J Bacteriol 188:7297-305. 2006
    ..This study has provided insight into the possible origins of C. difficile and its evolution that may have implications in disease control strategies...
  35. pmc Application of high-density array-based signature-tagged mutagenesis to discover novel Yersinia virulence-associated genes
    A V Karlyshev
    Department of Infectious Diseases, London School of Hygiene and Tropical Medicine, University of London, London WC1E 7HT, United Kingdom
    Infect Immun 69:7810-9. 2001
    ..The combination of optimized double tag sequences and high-density array hybridization technology offers improved performance, efficiency, and reliability over classical STM and permits quantitative analysis of data...
  36. ncbi request reprint The Campylobacter jejuni general glycosylation system is important for attachment to human epithelial cells and in the colonization of chicks
    A V Karlyshev
    London School of Hygiene and Tropical Medicine, University of London, Keppel Street, London EC1A 7HT, UK
    Microbiology 150:1957-64. 2004
    ..These results suggest that glycosylation is important for the attachment of C. jejuni to human and chicken host cells and imply a role for glycoproteins in the pathogenesis of C. jejuni...
  37. ncbi request reprint Biofilm formation in Campylobacter jejuni
    G W P Joshua
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Microbiology 152:387-96. 2006
    ..It is proposed that these poorly characterized forms of growth are important for the persistence of C. jejuni in the environment and may in part explain the high incidence of Campylobacter-associated food borne disease...
  38. doi request reprint Clostridium difficile--a continually evolving and problematic pathogen
    Lisa F Dawson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Infect Genet Evol 9:1410-7. 2009
    ..difficile to thrive. The rapid worldwide spread of the 017, 027 and 078 clones of C. difficile provides a valuable opportunity to study the very recent emergence of a bacterial pathogen-a rare chance to monitor evolution in action...
  39. pmc Helicobacter pylori pore-forming cytolysin orthologue TlyA possesses in vitro hemolytic activity and has a role in colonization of the gastric mucosa
    M C Martino
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    Infect Immun 69:1697-703. 2001
    ..These data clearly suggest a role in virulence for H. pylori TlyA, contrary to the suggestion that hemolytic activity is an in vitro phenomenon for this pathogen...
  40. ncbi request reprint Identification, cloning and initial characterisation of FeuPQ in Brucella suis: a new sub-family of two-component regulatory systems
    N Dorrell
    Department of Medical Microbiology, St Bartholomew s, London, UK
    FEMS Microbiol Lett 162:143-50. 1998
    ..The survival and multiplication of the mutant in macrophages was also unaffected. The FeuPQ regulon represents a newly characterised sub-family of response regulators...
  41. pmc Yersinia pestis pFra shows biovar-specific differences and recent common ancestry with a Salmonella enterica serovar Typhi plasmid
    M B Prentice
    Department of Medical Microbiology, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, United Kingdom
    J Bacteriol 183:2586-94. 2001
    ..pestis strains which distinguish Y. pestis bv. Orientalis strains from other biovars. We also present evidence for plasmid-associated DNA exchange between Y. pestis and the exclusively human pathogen Salmonella enterica serovar Typhi...
  42. pmc Development and application of an insertional system for gene delivery and expression in Campylobacter jejuni
    A V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, Keppel St, London WC1E 7HT, United Kingdom
    Appl Environ Microbiol 71:4004-13. 2005
    ..The principles developed in this study could be more widely applied for the manipulation of other bacteria that are refractory to genetic analysis...
  43. pmc Development of signature-tagged mutagenesis in Burkholderia pseudomallei to identify genes important in survival and pathogenesis
    J Cuccui
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, Keppel Street, London WC1E 7HT, United Kingdom
    Infect Immun 75:1186-95. 2007
    ..The refined signature-tagged mutagenesis approach developed in this study was used to efficiently identify attenuating mutants from this highly pathogenic species and could be applied to other organisms...
  44. ncbi request reprint A novel paralogous gene family involved in phase-variable flagella-mediated motility in Campylobacter jejuni
    Andrey V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, Keppel Street, London WC1E 7HT, UK
    Microbiology 148:473-80. 2002
    ..Reversible expression of flagella may be advantageous for the adaptation of C. jejunito the varied in vivo and ex vivo environments encountered during its life cycle, as well in evasion of the host immune response...
  45. ncbi request reprint Identification of N-acetylgalactosamine-containing glycoproteins PEB3 and CgpA in Campylobacter jejuni
    Dennis Linton
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK
    Mol Microbiol 43:497-508. 2002
    ..jejuni. Furthermore, we have identified two such glycoproteins, the first non-flagellin campylobacter glycoproteins to be identified, and demonstrated that their glycan components contain alpha-linked N-acetylgalactosamine residues...
  46. pmc Application of comparative phylogenomics to study the evolution of Yersinia enterocolitica and to identify genetic differences relating to pathogenicity
    Sarah L Howard
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK
    J Bacteriol 188:3645-53. 2006
    ..enterocolitica. Further analysis revealed that Y. enterocolitica does not cluster according to source (host)...
  47. doi request reprint Comparative analysis of BI/NAP1/027 hypervirulent strains reveals novel toxin B-encoding gene (tcdB) sequences
    Richard A Stabler
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    J Med Microbiol 57:771-5. 2008
    ....
  48. doi request reprint Comparative genome analysis and global phylogeny of the toxin variant Clostridium difficile PCR Ribotype 017 reveals the evolution of two independent sub-lineages
    M D Cairns
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT UK
    J Clin Microbiol . 2016
    ..Despite only having one toxin, RT017 strains have evolved in parallel from at least two independent sources and can readily transmit between continents...
  49. doi request reprint The importance of the Rcs phosphorelay in the survival and pathogenesis of the enteropathogenic yersiniae
    Stewart J Hinchliffe
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Microbiology 154:1117-31. 2008
    ..Phenotypic assays on defined mutants confirmed a role for the phosphorelay in these processes in both Y. pseudotuberculosis and Y. enterocolitica...
  50. ncbi request reprint Comparative phylogenomics of pathogenic bacteria by microarray analysis
    Nick Dorrell
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, United Kingdom
    Curr Opin Microbiol 8:620-6. 2005
    ..Recent results from such studies have generated insights into the evolution of bacterial pathogens, the levels of diversity and plasticity in the genome of a species, as well as the differences in virulence amongst pathogenic bacteria...
  51. ncbi request reprint Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses
    Andrey V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WCIE 7HT, UK
    Mol Microbiol 55:90-103. 2005
    ..Furthermore, we show the retention of two functional heptose isomerases in Campylobacter and the sharing of a phosphatase for both LOS and CPS heptose biosynthesis...
  52. ncbi request reprint Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity
    A V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, Keppel Street, London WC1E 7HT, UK
    Mol Microbiol 35:529-41. 2000
    ..We demonstrate for the first time that the previously described O-antigen of C. jejuni is a capsular polysaccharide and a common component of the thermostable antigen used for serotyping of C. jejuni...
  53. ncbi request reprint A flagellar-specific ATPase (FliI) is necessary for flagellar export in Helicobacter pylori
    P J Jenks
    Department of Medical Microbiology, St Bartholomew s Hospital, West Smithfield, London, UK
    FEMS Microbiol Lett 152:205-11. 1997
    ..The majority (> 99%) of mutant cells were completely aflagellate. These results suggest that FliI is a novel ATPase involved in flagellar export in H. pylori...
  54. pmc Nucleotide sequence of two genes from Helicobacter pylori encoding for urease subunits
    C L Clayton
    Department of Medical Microbiology, St Bartholomew s Hospital Medical College, West Smithfield, London, UK
    Nucleic Acids Res 18:362. 1990
  55. pmc Demonstration of polysaccharide capsule in Campylobacter jejuni using electron microscopy
    A V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, London WC1E 7HT, United Kingdom
    Infect Immun 69:5921-4. 2001
    ..jejuni cells with the cationic dye Alcian blue and demonstrate for the first time by electron microscopy that C. jejuni cells produce a polysaccharide capsule that is retained in the coccoid form but is absent in a kpsM mutant...
  56. ncbi request reprint Structural characterization of lipo-oligosaccharide (LOS) from Yersinia pestis: regulation of LOS structure by the PhoPQ system
    Paul G Hitchen
    Department of Biological Sciences, Wolfson Building, Imperial College, London, SW7 2AY, UK
    Mol Microbiol 44:1637-50. 2002
    ..Thus, LOS modification is under the control of the PhoPQ regulatory system and the ability to alter LOS structure may be required for survival of Y. pestis within the mammalian and/or flea host...
  57. pmc Comparative phylogenomics of the food-borne pathogen Campylobacter jejuni reveals genetic markers predictive of infection source
    Olivia L Champion
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    Proc Natl Acad Sci U S A 102:16043-8. 2005
    ..jejuni infection that may have implications in disease-control strategies. The comparative phylogenomics approach described provides a robust methodological prototype that should be applicable to other microbes...
  58. pmc Genomic Epidemiology of a Protracted Hospital Outbreak Caused by a Toxin A-Negative Clostridium difficile Sublineage PCR Ribotype 017 Strain in London, England
    M D Cairns
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom UCL Centre for Clinical Microbiology, University College London, London, United Kingdom Public Health Laboratory London, Health Protection Agency, Division of Infection, The Royal London Hospital, London, United Kingdom
    J Clin Microbiol 53:3141-7. 2015
    ..By utilization of WGS technologies to identify SNPs and the evolution of clonal strains, the transmission of outbreaks caused by near-identical isolates can be retraced and identified. ..
  59. ncbi request reprint Degenerate PCR primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria
    B W Wren
    Department of Medical Microbiology, St Bartholomew s Hospital Medical College, West Smithfield, London, UK
    FEMS Microbiol Lett 78:287-91. 1992
    ..Mutagenesis of the newly identified genes should allow us to determine their function and the genes under their control...
  60. ncbi request reprint Immunization with a DNA vaccine expressing a truncated form of varicella zoster virus glycoprotein E
    U A Hasan
    Department of Immunology, St Bartholomew s and the Royal London School of Medicine and Dentistry, EC1A 7BE, London, UK
    Vaccine 20:1308-15. 2002
    ..This observation indicates that immunization with a truncated form of a gene may induce immune modulation, a phenomenon that should be taken into account for the design of vaccines...
  61. ncbi request reprint Mucosal immunization with a urease B DNA vaccine induces innate and cellular immune responses against Helicobacter pylori
    Caterina Hatzifoti
    Department of Immunology, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    Helicobacter 11:113-22. 2006
    ..pylori vaccine studies is the type of immune response required to control infection. A new approach in H. pylori vaccinology is the administration of DNA vaccines, which has included heat-shock protein and catalase DNA vaccines...
  62. ncbi request reprint The second century of Campylobacter research: recent advances, new opportunities and old problems
    Nick Dorrell
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Curr Opin Infect Dis 20:514-8. 2007
    ..In particular it is still unclear how C. jejuni infection causes diarrhoea...
  63. doi request reprint Assessing the role of p-cresol tolerance in Clostridium difficile
    Lisa F Dawson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    J Med Microbiol 57:745-9. 2008
    ..difficile strains including 630, CF4 and CD196. Surprising, it was shown that Clostridium sordellii also has a high tolerance to p-cresol, suggesting an overlap in the tolerance pathways in these clostridial species...
  64. ncbi request reprint The Campylobacter jejuni glycome
    Andrey V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK
    FEMS Microbiol Rev 29:377-90. 2005
    ..jejuni...
  65. ncbi request reprint Functional analysis of the Campylobacter jejuni N-linked protein glycosylation pathway
    Dennis Linton
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Mol Microbiol 55:1695-703. 2005
    ..These findings provide a framework for understanding the process of N-linked protein glycosylation in Bacteria and for devising strategies to exploit this system for glycoengineering...
  66. pmc Campylobacter jejuni glycosylation island important in cell charge, legionaminic acid biosynthesis, and colonization of chickens
    Sarah L Howard
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    Infect Immun 77:2544-56. 2009
    ..The discovery of molecular mechanisms influencing the persistence of C. jejuni in poultry aids the rational design of approaches to control this problematic pathogen in the food chain...
  67. ncbi request reprint Genes to genetic immunization: identification of bacterial vaccine candidates
    Elaine Allan
    Microbiology Department, Eastman Dental Institute, University College London, London, UK
    Methods 31:193-8. 2003
    ..In this chapter, we review approaches that exploit whole genome sequence data to identify potential virulence determinants and vaccine antigens...
  68. doi request reprint Recent advances in systems microbiology
    Emily Kay
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom
    Curr Opin Microbiol 12:577-81. 2009
    ..The challenge now is to use these data in conjunction with modelling techniques to extend our knowledge of bacterial systems and to be able to experimentally verify in silico predictions...
  69. ncbi request reprint The RovA regulons of Yersinia enterocolitica and Yersinia pestis are distinct: evidence that many RovA-regulated genes were acquired more recently than the core genome
    Jason S Cathelyn
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, Saint Louis, MO 63110, USA
    Mol Microbiol 66:189-205. 2007
    ..This repression can be selectively relieved by regulators such as RovA, and the observation that nearly all RovA-activated genes are repressed by H-NS is consistent with this model...
  70. ncbi request reprint The complete genome sequence of Francisella tularensis, the causative agent of tularemia
    Par Larsson
    Swedish Defence Research Agency, SE 901 82 Umea, Sweden
    Nat Genet 37:153-9. 2005
    ..tularensis is an obligate host-dependent bacterium in its natural life cycle. Our results have implications for our understanding of how highly virulent human pathogens evolve and will expedite strategies to combat them...
  71. ncbi request reprint Invertebrates as a source of emerging human pathogens
    Nicholas R Waterfield
    Centre for Molecular Microbiology, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Nat Rev Microbiol 2:833-41. 2004
    ..This article examines the available evidence for this concept by examining pairs of closely related bacteria, one of which is benign, but insect associated, and one of which is a human pathogen...
  72. ncbi request reprint Comparative genome analyses of the pathogenic Yersiniae based on the genome sequence of Yersinia enterocolitica strain 8081
    Nicholas R Thomson
    The Pathogen Sequencing Unit, Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Cambridge, UK
    Adv Exp Med Biol 603:2-16. 2007
    ..enterocolitica gene functions and important insights into the intra-species differences between the high, low and non-pathogenic Y. enterocolitica biotypes...
  73. pmc Serotype differences and lack of biofilm formation characterize Yersinia pseudotuberculosis infection of the Xenopsylla cheopis flea vector of Yersinia pestis
    David L Erickson
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, 903 South 4th St, Hamilton, MT 59840, USA
    J Bacteriol 188:1113-9. 2006
    ..pseudotuberculosis progenitor of Y. pestis extended its pre-existing ex vivo biofilm-forming ability to the flea gut environment, thus enabling proventricular blockage and efficient flea-borne transmission...
  74. ncbi request reprint Campylobacter jejuni-mediated disease pathogenesis: an update
    Matthias Zilbauer
    Infectious Diseases and Microbiology Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
    Trans R Soc Trop Med Hyg 102:123-9. 2008
    ..Here recent developments in C. jejuni research, with a particular focus on disease pathogenesis including early host immune responses, are highlighted...
  75. ncbi request reprint Numerical analysis of DNA microarray data of Campylobacter jejuni strains correlated with survival, cytolethal distending toxin and haemolysin analyses
    Stephen L W On
    Danish Institute of Food and Veterinary Research, Bulowsvej 27, DK 1790, Copenhagen V, Denmark
    Int J Med Microbiol 296:353-63. 2006
    ..jejuni genomic content, particularly in surface-coding regions, and its capacity for environmental survival, and may help explain why certain serotypes are more commonly reported in human disease...
  76. ncbi request reprint The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
    Mohammed Sebaihia
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Genet 38:779-86. 2006
    ..The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism...
  77. ncbi request reprint N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli
    Michael Wacker
    Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology, Zurich, CH 8092 Zurich, Switzerland
    Science 298:1790-3. 2002
    ..coli opens up the possibility of engineering permutations of recombinant glycan structures for research and industrial applications...
  78. ncbi request reprint Signature-tagged mutagenesis of Yersinia pestis
    Petra C F Oyston
    London School of Hygiene and Tropical Medicine, University of London
    Adv Exp Med Biol 529:39-41. 2003
  79. pmc Adaptation of Campylobacter jejuni NCTC11168 to high-level colonization of the avian gastrointestinal tract
    Michael A Jones
    Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom
    Infect Immun 72:3769-76. 2004
    ..This study showed that there was colonization of the avian intestinal tract by a Campylobacter strain having a known genome sequence, and it provides a model for colonization and persistence studies with specific mutations...
  80. ncbi request reprint Helicobacter pylori adherence to gastric epithelial cells: a role for non-adhesin virulence genes
    Zun Wu Zhang
    Division of Surgery, Bristol Royal Infirmary, University of Bristol, UK
    J Med Microbiol 51:495-502. 2002
    ..Mutations in these genes may affect H. pylori pathogenicity by reducing either the ability of the bacteria to attach to gastric epithelial cells or the intensity of bacteria-host cell interactions...
  81. ncbi request reprint Bacterial pathogenomics
    Mark J Pallen
    Centre for Systems Biology, University of Birmingham, Birmingham B15 2TT, UK
    Nature 449:835-42. 2007
    ..It has also become evident that even the definitions for 'pathogen' and 'virulence factor' need to be re-evaluated...
  82. ncbi request reprint Campylobacter--a tale of two protein glycosylation systems
    Christine M Szymanski
    Institute for Biological Sciences, National Research Council, Ottawa, Ontario, K1A 0R6, Canada
    Trends Microbiol 11:233-8. 2003
    ..Thus, Campylobacter provides a unique model system for the elucidation and exploitation of glycoprotein biosynthesis...
  83. ncbi request reprint Revised nomenclature of Clostridium difficile toxins and associated genes
    Maja Rupnik
    Department of Biology, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
    J Med Microbiol 54:113-7. 2005
    ..difficile toxins and other related large clostridial toxins produced by Clostridium sordellii and Clostridium novyi, and corresponding toxin genes, as well as toxin production types of C. difficile strains...
  84. ncbi request reprint Protein glycosylation in bacterial mucosal pathogens
    Christine M Szymanski
    Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada
    Nat Rev Microbiol 3:225-37. 2005
    ....
  85. pmc The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081
    Nicholas R Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Genet 2:e206. 2006
    ..Moreover, wider investigations looking at the patterns of gene loss and gain in the Yersinia have highlighted common themes in the genome evolution of other human enteropathogens...
  86. ncbi request reprint The identification of two protective DNA vaccines from a panel of five plasmid constructs encoding Brucella melitensis 16M genes
    Nicola J Commander
    Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
    Vaccine 25:43-54. 2007
    ..This candidate has not previously been investigated with regard to Brucella virulence or pathogenesis. This study is the first report to identify the Brucella invasion protein B (BMEI1584) as a novel protective antigen for brucellosis...
  87. ncbi request reprint Clostridium difficile comes of age
    Brendan W Wren
    Future Microbiol 1:243-5. 2006
  88. ncbi request reprint Commonality and biosynthesis of the O-methyl phosphoramidate capsule modification in Campylobacter jejuni
    David J McNally
    Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada
    J Biol Chem 282:28566-76. 2007
    ..These findings establish the importance of the MeOPN as a diagnostic marker and therapeutic target for C. jejuni and set the groundwork for future studies aimed at the detailed elucidation of the MeOPN biosynthetic pathway...
  89. doi request reprint Microarray analysis of the transcriptional responses of Clostridium difficile to environmental and antibiotic stress
    Jenny E Emerson
    Centre for Molecular Microbiology and Infection, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, UK
    J Med Microbiol 57:757-64. 2008
    ..This study provides a starting point for detailed analyses of numerous genes whose expression is affected by stress and may therefore be involved in adaptation to the host environment...
  90. pmc Intestinal innate immunity to Campylobacter jejuni results in induction of bactericidal human beta-defensins 2 and 3
    Matthias Zilbauer
    Department of Infectious Diseases and Microbiology, Institute of Child Health, London WC1N 1EH, United Kingdom
    Infect Immun 73:7281-9. 2005
    ..jejuni or the ability of C. jejuni to survive exposure to recombinant beta-defensins. This study suggests a major role for this family of antimicrobial peptides in the innate immune defense against this human pathogen...
  91. pmc Explorative multifactor approach for investigating global survival mechanisms of Campylobacter jejuni under environmental conditions
    Birgitte Moen
    Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Norway
    Appl Environ Microbiol 71:2086-94. 2005
    ..Basic knowledge about the survival mechanisms is of fundamental importance in preventing transmission of this bacterium through the food chain...
  92. ncbi request reprint Deletion of a previously uncharacterized flagellar-hook-length control gene fliK modulates the sigma54-dependent regulon in Campylobacter jejuni
    Nahid Kamal
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Microbiology 153:3099-111. 2007
    ..Together the data clearly define the roles of the alternative sigma factors RpoN and FliA in flagellar biogenesis in C. jejuni, and identify additional putative members of their respective regulons...
  93. ncbi request reprint A major role for intestinal epithelial nucleotide oligomerization domain 1 (NOD1) in eliciting host bactericidal immune responses to Campylobacter jejuni
    Matthias Zilbauer
    Infectious Diseases and Microbiology Unit, Institute of Child Health, 30 Guilford St, London WC1N 1EH, UK
    Cell Microbiol 9:2404-16. 2007
    ..jejuni, thus highlighting a critical role for NOD1-mediated antimicrobial defence mechanism(s) in combating this infection at the gastrointestinal mucosal surface...