Mark P Stevens

Summary

Affiliation: Institute for Animal Health
Country: UK

Publications

  1. pmc Cloning, expression, and characterization of fimbrial operon F9 from enterohemorrhagic Escherichia coli O157:H7
    Alison S Low
    Zoonotic and Animal Pathogens Research Laboratory, Centre for Infectious Diseases, Chancellor s Building, University of Edinburgh, 49 Little France Crescent, Edinburgh, EH16 4SB, United Kingdom
    Infect Immun 74:2233-44. 2006
  2. pmc Global transcriptional profiling of Burkholderia pseudomallei under salt stress reveals differential effects on the Bsa type III secretion system
    Pornpan Pumirat
    Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    BMC Microbiol 10:171. 2010
  3. pmc Molecular insights into farm animal and zoonotic Salmonella infections
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
    Philos Trans R Soc Lond B Biol Sci 364:2709-23. 2009
  4. pmc Mutation of toxB and a truncated version of the efa-1 gene in Escherichia coli O157:H7 influences the expression and secretion of locus of enterocyte effacement-encoded proteins but not intestinal colonization in calves or sheep
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom
    Infect Immun 72:5402-11. 2004
  5. ncbi Options for the control of enterohaemorrhagic Escherichia coli in ruminants
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Compton, Newbury RG20 7NN, UK
    Microbiology 148:3767-78. 2002
  6. ncbi Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, UK
    Microbiology 150:2669-76. 2004
  7. pmc Systemic translocation of Salmonella enterica serovar Dublin in cattle occurs predominantly via efferent lymphatics in a cell-free niche and requires type III secretion system 1 (T3SS-1) but not T3SS-2
    Gillian D Pullinger
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom
    Infect Immun 75:5191-9. 2007
  8. ncbi Role in virulence and protective efficacy in pigs of Salmonella enterica serovar Typhimurium secreted components identified by signature-tagged mutagenesis
    Sonya C Carnell
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
    Microbiology 153:1940-52. 2007
  9. pmc Role of intimin-tir interactions and the tir-cytoskeleton coupling protein in the colonization of calves and lambs by Escherichia coli O157:H7
    Isabella Vlisidou
    Institute for Animal Health, High Street, Compton, Berkshire RG20 7NN, United Kingdom
    Infect Immun 74:758-64. 2006
  10. pmc Efa1 influences colonization of the bovine intestine by shiga toxin-producing Escherichia coli serotypes O5 and O111
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom
    Infect Immun 70:5158-66. 2002

Detail Information

Publications57

  1. pmc Cloning, expression, and characterization of fimbrial operon F9 from enterohemorrhagic Escherichia coli O157:H7
    Alison S Low
    Zoonotic and Animal Pathogens Research Laboratory, Centre for Infectious Diseases, Chancellor s Building, University of Edinburgh, 49 Little France Crescent, Edinburgh, EH16 4SB, United Kingdom
    Infect Immun 74:2233-44. 2006
    ....
  2. pmc Global transcriptional profiling of Burkholderia pseudomallei under salt stress reveals differential effects on the Bsa type III secretion system
    Pornpan Pumirat
    Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    BMC Microbiol 10:171. 2010
    ..pseudomallei. We therefore investigated the global transcriptional response of B. pseudomallei to increased salinity using microarray analysis...
  3. pmc Molecular insights into farm animal and zoonotic Salmonella infections
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
    Philos Trans R Soc Lond B Biol Sci 364:2709-23. 2009
    ..Here we review current knowledge of the bacterial factors influencing colonization of food-producing animals by Salmonella and the basis of host range, differential virulence and zoonotic potential...
  4. pmc Mutation of toxB and a truncated version of the efa-1 gene in Escherichia coli O157:H7 influences the expression and secretion of locus of enterocyte effacement-encoded proteins but not intestinal colonization in calves or sheep
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom
    Infect Immun 72:5402-11. 2004
    ..Mutation of tir caused a significant reduction in fecal shedding of E. coli O157:H7 in calves, indicating that the formation of AE lesions is important for colonization of the bovine intestine...
  5. ncbi Options for the control of enterohaemorrhagic Escherichia coli in ruminants
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Compton, Newbury RG20 7NN, UK
    Microbiology 148:3767-78. 2002
  6. ncbi Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, UK
    Microbiology 150:2669-76. 2004
    ..Mice inoculated with the B. pseudomallei bipD mutant were partially protected against subsequent challenge with wild-type B. pseudomallei. However, immunization of mice with purified BipD protein was not protective...
  7. pmc Systemic translocation of Salmonella enterica serovar Dublin in cattle occurs predominantly via efferent lymphatics in a cell-free niche and requires type III secretion system 1 (T3SS-1) but not T3SS-2
    Gillian D Pullinger
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom
    Infect Immun 75:5191-9. 2007
    ..This is the first description of the mode and genetics of systemic translocation of serovar Dublin in its natural host...
  8. ncbi Role in virulence and protective efficacy in pigs of Salmonella enterica serovar Typhimurium secreted components identified by signature-tagged mutagenesis
    Sonya C Carnell
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
    Microbiology 153:1940-52. 2007
    ..The data provide valuable information for the control of Salmonella in pigs...
  9. pmc Role of intimin-tir interactions and the tir-cytoskeleton coupling protein in the colonization of calves and lambs by Escherichia coli O157:H7
    Isabella Vlisidou
    Institute for Animal Health, High Street, Compton, Berkshire RG20 7NN, United Kingdom
    Infect Immun 74:758-64. 2006
    ..However, an E. coli O157:H7 tccP mutant induced typical attaching and effacing lesions in a bovine ligated ileal loop model of infection, suggesting that TccP-independent mechanisms of actin assembly may operate in vivo...
  10. pmc Efa1 influences colonization of the bovine intestine by shiga toxin-producing Escherichia coli serotypes O5 and O111
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom
    Infect Immun 70:5158-66. 2002
    ..Efa1 is only the second STEC factor shown to influence carriage of the bacteria in the bovine intestine. Our data may have implications for strategies to reduce the prevalence of STEC in cattle...
  11. pmc Identification of enterohemorrhagic Escherichia coli O26:H- genes required for intestinal colonization in calves
    Pauline M van Diemen
    Mammalian Enteric Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom
    Infect Immun 73:1735-43. 2005
    ..These observations have important implications for the design of vaccines to control these important zoonotic pathogens...
  12. pmc Net replication of Salmonella enterica serovars Typhimurium and Choleraesuis in porcine intestinal mucosa and nodes is associated with their differential virulence
    Susan M Paulin
    Enteric Bacterial Pathogens Laboratory, Division of Microbiology, Institute for Animal Health, Compton, Berkshire, United Kingdom
    Infect Immun 75:3950-60. 2007
    ....
  13. pmc Norepinephrine augments Salmonella enterica-induced enteritis in a manner associated with increased net replication but independent of the putative adrenergic sensor kinases QseC and QseE
    Gillian D Pullinger
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom
    Infect Immun 78:372-80. 2010
    ....
  14. doi Efa-1/LifA mediates intestinal colonization of calves by enterohaemorrhagic Escherichia coli O26 : H- in a manner independent of glycosyltransferase and cysteine protease motifs or effects on type III secretion
    Victoria Deacon
    Enteric Bacterial Pathogens Laboratory, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
    Microbiology 156:2527-36. 2010
    ..coli O127 : H6 abolished lymphostatin activity. Our data uncouple the role of Efa-1/LifA in calf colonization from effects on type III secretion and reinforce the potential for pathotype- and serotype-specific phenotypes...
  15. doi Evaluation of live-attenuated Salmonella vaccines expressing Campylobacter antigens for control of C. jejuni in poultry
    Anthony M Buckley
    Division of Microbiology, Institute for Animal Health, Berkshire, United Kingdom
    Vaccine 28:1094-105. 2010
    ..Our data are rendered highly relevant by use of the target host in large numbers and support the potential of CjaA- and Peb1A-based vaccines for control of C. jejuni in poultry...
  16. pmc The neuroendocrine stress hormone norepinephrine augments Escherichia coli O157:H7-induced enteritis and adherence in a bovine ligated ileal loop model of infection
    Isabella Vlisidou
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, RG20 7NN, United Kingdom
    Infect Immun 72:5446-51. 2004
    ..Norepinephrine modulation of enteritis and adherence was dependent on the ability of E. coli O157:H7 to form attaching and effacing lesions...
  17. ncbi Identification and characterization of EspK, a type III secreted effector protein of enterohaemorrhagic Escherichia coli O157:H7
    Isabella Vlisidou
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire, UK
    FEMS Microbiol Lett 263:32-40. 2006
    ..Inactivation of an orthologue of espK in the murine AE pathogen Citrobacter rodentium did not impair intestinal colonization in mice...
  18. pmc Identification of Salmonella enterica serovar Dublin-specific sequences by subtractive hybridization and analysis of their role in intestinal colonization and systemic translocation in cattle
    Gillian D Pullinger
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom
    Infect Immun 76:5310-21. 2008
    ..SDI-1 mutation did not impair invasion, intramacrophage survival, or virulence in mice, implying that SDI-1 does not influence fitness per se and may act in a host-specific manner...
  19. ncbi Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis
    Francis Dziva
    Mammalian Enteric Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK
    Microbiology 150:3631-45. 2004
    ..Our data provide valuable new information for the development of intervention strategies...
  20. ncbi An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, UK
    Mol Microbiol 46:649-59. 2002
    ..These findings indicate that the Bsa type III secretion system plays an essential role in modulating the intracellular behaviour of B. pseudomallei...
  21. doi Role of two-component sensory systems of Salmonella enterica serovar Dublin in the pathogenesis of systemic salmonellosis in cattle
    Gillian D Pullinger
    Enteric Bacterial Pathogens Laboratory, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
    Microbiology 156:3108-22. 2010
    ..Spatial roles were assigned to eight S. Dublin SKs, but most were not essential for intestinal or systemic infection of the target host...
  22. pmc 6-hydroxydopamine-mediated release of norepinephrine increases faecal excretion of Salmonella enterica serovar Typhimurium in pigs
    Gillian D Pullinger
    Enteric Bacterial Pathogens Laboratory, Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom
    Vet Res 41:68. 2010
    ..Our data support the notion that stress-related catecholamines modulate the interaction of enteric bacterial pathogens with their hosts...
  23. pmc Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant
    Joanne M Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, UK
    J Bacteriol 187:7857-62. 2005
    ..pseudomallei bimA mutant. While the BimA homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind Arp3...
  24. ncbi Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, RG20 7NN, UK
    Mol Microbiol 56:40-53. 2005
    ....
  25. pmc Identification of novel genes and pathways affecting Salmonella type III secretion system 1 using a contact-dependent hemolysis assay
    Terry R Field
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom
    J Bacteriol 190:3393-8. 2008
    ..Our data suggest that previously unrecognized factors such as type I fimbriae may modulate the expression, activity, or deployment of this key virulence factor...
  26. ncbi Vaccination of calves with EspA, a key colonisation factor of Escherichia coli O157:H7, induces antigen-specific humoral responses but does not confer protection against intestinal colonisation
    Francis Dziva
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom
    Vet Microbiol 123:254-61. 2007
    ..coli O157:H7 upon experimental infection...
  27. pmc SiiE is secreted by the Salmonella enterica serovar Typhimurium pathogenicity island 4-encoded secretion system and contributes to intestinal colonization in cattle
    Eirwen Morgan
    Division of Microbiology, Institute for Animal Health, Compton, Nr Newbury, Berkshire, RG20 7NN, United Kingdom
    Infect Immun 75:1524-33. 2007
    ..SiiE is surface expressed, and its production is regulated by HilA. SiiE and SiiF influence colonization in cattle and the invasion of bovine enterocytes...
  28. ncbi Functional analysis of lymphostatin homologues in enterohaemorrhagic Escherichia coli
    Abu Bakr Abu-Median
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK
    FEMS Microbiol Lett 258:43-9. 2006
    ..While a Shiga toxin-negative E. coli O157:H7 strain was shown to possess lymphostatin-like activity, deletion of efa1' or toxB, singly or in combination, failed to significantly relieve the inhibitory effect...
  29. ncbi Identification of host-specific colonization factors of Salmonella enterica serovar Typhimurium
    Eirwen Morgan
    Division of Microbiology, Institute for Animal Health, Compton, Nr Newbury, RG20 7NN, UK
    Mol Microbiol 54:994-1010. 2004
    ..We conclude that S. Typhimurium uses different strategies to colonize calves and chicks. This has major implications for vaccine design...
  30. pmc Analysis of the role of 13 major fimbrial subunits in colonisation of the chicken intestines by Salmonella enterica serovar Enteritidis reveals a role for a novel locus
    Debra J Clayton
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire, UK
    BMC Microbiol 8:228. 2008
    ..Here we report the first systematic analysis of each chromosomally-encoded major fimbrial subunit of S. Enteritidis in intestinal colonisation of chickens...
  31. pmc Intimin, tir, and shiga toxin 1 do not influence enteropathogenic responses to shiga toxin-producing Escherichia coli in bovine ligated intestinal loops
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, United Kingdom
    Infect Immun 70:945-52. 2002
    ..This may have implications for strategies to reduce STEC carriage in cattle...
  32. pmc Analysis of Salmonella enterica serotype-host specificity in calves: avirulence of S. enterica serotype gallinarum correlates with bacterial dissemination from mesenteric lymph nodes and persistence in vivo
    Susan M Paulin
    Institute for Animal Health, Compton, Newbury, Berkshire, RG20 7NN, United Kingdom
    Infect Immun 70:6788-97. 2002
    ....
  33. pmc A Burkholderia pseudomallei type III secreted protein, BopE, facilitates bacterial invasion of epithelial cells and exhibits guanine nucleotide exchange factor activity
    Mark P Stevens
    Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom
    J Bacteriol 185:4992-6. 2003
    ..Consistent with this notion, BopE expressed in eukaryotic cells induced rearrangements in the subcortical actin cytoskeleton, and purified BopE exhibited guanine nucleotide exchange factor activity for Cdc42 and Rac1 in vitro...
  34. ncbi Exploitation of host cells by Burkholderia pseudomallei
    Mark P Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, United Kingdom
    Int J Med Microbiol 293:549-55. 2004
    ..Here we review current understanding of the molecular mechanisms underlying these processes...
  35. ncbi EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells
    Francis Dziva
    Division of Microbiology, Institute for Animal Health, Compton, Newbury, Berkshire, UK
    FEMS Microbiol Lett 271:258-64. 2007
    ..Data of this study add to the growing body of evidence that cytotoxins facilitate intestinal colonization by EHEC...
  36. doi Salicylidene acylhydrazide-mediated inhibition of type III secretion system-1 in Salmonella enterica serovar Typhimurium is associated with iron restriction and can be reversed by free iron
    Abigail N Layton
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire, UK
    FEMS Microbiol Lett 302:114-22. 2010
    ..We show that INP0403 restricts iron availability to Salmonella, and that inhibition of T3S system-1 by INP0403 is, at least in part, reversible by exogenous iron and independent of the iron response regulator Fur...
  37. pmc Sequencing and functional annotation of avian pathogenic Escherichia coli serogroup O78 strains reveal the evolution of E. coli lineages pathogenic for poultry via distinct mechanisms
    Francis Dziva
    Enteric Bacterial Pathogens Laboratory, Institute for Animal Health, Compton, Berkshire, United Kingdom
    Infect Immun 81:838-49. 2013
    ..coli strains adapted to cause avian or human disease via acquisition of distinct virulence genes...
  38. pmc Inhibition of type III secretion in Salmonella enterica serovar Typhimurium by small-molecule inhibitors
    Debra L Hudson
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom
    Antimicrob Agents Chemother 51:2631-5. 2007
    ..have recently been identified. Here we show that two such compounds inhibit Salmonella T3SS-1, preventing secretion of T3SS-1 effectors, invasion of cultured epithelial cells, and enteritis in vivo...
  39. doi Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts
    Francis Dziva
    Division of Microbiology, Institute for Animal Health, Compton, Newbury, Berkshire, UK
    Avian Pathol 37:355-66. 2008
    ..Collectively, such information provides the basis for the development or improvement of strategies to control APEC infections in the food-producing avian species...
  40. ncbi Actin-dependent movement of bacterial pathogens
    Joanne M Stevens
    Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, RG20 7NN, UK
    Nat Rev Microbiol 4:91-101. 2006
    ..The molecular basis of actin-based motility of these bacterial pathogens reveals novel insights about bacterial pathogenesis and fundamental host-cell pathways...
  41. ncbi Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis
    Jason P Chattoo
    Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom
    J Virol Methods 135:288-91. 2006
    ..The application of the screening method to identify some of the non-essential genes for MDV replication in vitro is described...
  42. pmc Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge
    Jean Remy Sadeyen
    The Pirbright Institute, Compton, Newbury, Berkshire RG20 7NN, UK
    Vet Res 45:19. 2014
    ..Such data inform the rational design of strategies to control a recalcitrant endemic disease of poultry...
  43. doi Interleukin-8 induction by Burkholderia pseudomallei can occur without Toll-like receptor signaling but requires a functional type III secretion system
    Chung Shii Hii
    Department of Biochemistry, National University of Singapore, Singapore
    J Infect Dis 197:1537-47. 2008
    ..Thus, in cells with no or low expression of TLRs, such as mucosal epithelial cells, B. pseudomallei can induce IL-8 via NF-kappaB and MAPK pathways, aided by Bsa T3SS...
  44. pmc Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo
    Francis Girard
    Division of Cell and Molecular Biology, Imperial College London, London, UK
    Appl Environ Microbiol 73:3084-90. 2007
    ..We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization...
  45. pmc Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of Escherichia coli O157:H7 to porcine cecum and colon
    Chunsheng Chen
    Department of Veterinary and Biomedical Sciences, University of Minnesota, 1988 Fitch Avenue, St Paul, MN 55108 6010, USA
    Eur J Pharmacol 539:116-24. 2006
    ..This in turn may alter host susceptibility to enteric infections...
  46. ncbi Human intestinal tissue tropism of intimin epsilon O103 Escherichia coli
    Robert J Fitzhenry
    Centre for Paediatric Gastroenterology, Lower 3rd Floor, Royal Free Hospital, Pond St, NW3 2QG, London, UK
    FEMS Microbiol Lett 218:311-6. 2003
    ..This is similar to the tissue tropism shown by intimin gamma enterohaemorrhagic (EHEC) O157:H7, but distinct from that of intimin alpha enteropathogenic (EPEC) O127:H6...
  47. ncbi Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli
    Tadasuke Ooka
    Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
    FEMS Microbiol Lett 271:126-35. 2007
    ..TccP2 of human and animal strains show high level of sequence identity. It is shown that most strains carry a tir gene encoding Tir-Yp. In addition the authors identified two new variants of tir genes in EPEC O104:H12 and NT:H19 strains...
  48. ncbi A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins
    Ashraful Haque
    London School of Hygiene and Tropical Medicine, London, United Kingdom
    J Infect Dis 194:1241-8. 2006
    ..CD4+ and CD8+ cell depletion studies demonstrated that CD4+ cells, but not CD8+ cells, mediated this protection in vivo. Thus, CD4+ T cells can mediate vaccine-induced immunity to experimental melioidosis...
  49. ncbi Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection
    Christian Menge
    Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University, D 35392 Giessen, Germany
    J Med Microbiol 53:573-9. 2004
    ..These studies indicate that the ligated ileal loop model will be a useful tool to dissect the mechanisms underlying suppression of mucosal inflammation by EHEC in the reservoir host...
  50. pmc Regulators encoded in the Escherichia coli type III secretion system 2 gene cluster influence expression of genes within the locus for enterocyte effacement in enterohemorrhagic E. coli O157:H7
    Lihong Zhang
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, Medical School, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Infect Immun 72:7282-93. 2004
    ..quot; It also seems likely that variations in the ETT2 regulator repertoire might account for strain-to-strain variation in secretion of LEE-encoded proteins...
  51. pmc EspJ is a prophage-carried type III effector protein of attaching and effacing pathogens that modulates infection dynamics
    Sivan Dahan
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 73:679-86. 2005
    ....
  52. pmc Role of NleH, a type III secreted effector from attaching and effacing pathogens, in colonization of the bovine, ovine, and murine gut
    Cordula Hemrajani
    Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 76:4804-13. 2008
    ..Consistent with this, we found that the nleH mutant triggered a significantly lower tumor necrosis factor alpha response than the wild-type strain...
  53. pmc Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens
    Olivier Marches
    CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
    Infect Immun 73:8411-7. 2005
    ..We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization...
  54. pmc Prevalence and sequence diversity of a factor required for actin-based motility in natural populations of Burkholderia species
    Chayada Sitthidet
    Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Pran Nok Road, Bangkoknoi, Bangkok 10700, Thailand
    J Clin Microbiol 46:2418-22. 2008
    ..A geographically restricted subset of B. pseudomallei isolates harbored a B. mallei-like bimA allele (12.1%), confounding a differential diagnostic test based on amplification of species-specific bimA regions...
  55. ncbi Biophysical characterization of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei
    Abhishek Upadhyay
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Biochim Biophys Acta 1698:111-9. 2004
    ..These properties are likely to be advantageous for a secreted bacterial effector protein...
  56. doi Amino acid-dependent growth of Campylobacter jejuni: key roles for aspartase (AspA) under microaerobic and oxygen-limited conditions and identification of AspB (Cj0762), essential for growth on glutamate
    Edward Guccione
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 69:77-93. 2008
    ..Together, our data highlight the dual function of aspartase in C. jejuni and suggest a role during growth in the avian gut...
  57. pmc Competing isogenic Campylobacter strains exhibit variable population structures in vivo
    Chris Coward
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, United Kingdom
    Appl Environ Microbiol 74:3857-67. 2008
    ..These factors render simple models of colonization which do not take them into account inappropriate for Campylobacter and impact the planning and interpretation of competition experiments using this organism...