M W Shirley
Affiliation: Institute for Animal Health
- Eimeria tenella: infection with a single sporocyst gives a clonal populationM W Shirley
Institute for Animal Health, Compton Laboratory, Nr Newbury, Berks, UK
Parasitology 112:523-8. 1996..EAch of the 5 polymorphic DNA markers used segregated independently within the "recombinant' parasites and only 1 of the parental forms was inherited in 22 of the populations aimed to have been established from a single sporocyst...
- Live attenuated vaccines against avian coccidiosis: Success with precocious and egg-adapted lines of EimeriaM W Shirley
Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berkshire, UK
Parasitol Today 13:481-4. 1997..Here, Martin Shirley and Petr Bedrník explain the basis of these recent vaccines and discuss their use...
- The biology of avian Eimeria with an emphasis on their control by vaccinationMartin W Shirley
Institute for Animal Health, Compton Laboratory, Compton Nr Newbury, Berks RG20 7NN, UK
Adv Parasitol 60:285-330. 2005..Although Eimeria are effective immunogens, it is probable that to date none of the antigens that induce potent protective immune responses during the course of natural infection has been isolated...
- The Eimeria genome projects: a sequence of eventsMartin W Shirley
Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berkshire RG20 7NN, UK
Trends Parasitol 20:199-201. 2004
- A genetic linkage map of the apicomplexan protozoan parasite Eimeria tenellaM W Shirley
Institute for Animal Health, Compton Laboratory, Compton, Near Newbury, Berkshire, RG20 7NN UK
Genome Res 10:1587-93. 2000..A table with the segregation data is available as an online supplement at http://www.genome.org.]..
- The genome of Eimeria spp., with special reference to Eimeria tenella--a coccidium from the chickenM W Shirley
Institute for Animal Health, Compton Laboratory, Compton, Newbury, UK
Int J Parasitol 30:485-93. 2000..No large-scale genomic DNA sequencing projects have been reported...
- Integrating genetics and genomics to identify new leads for the control of Eimeria sppM W Shirley
Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berks, UK
Parasitology 128:S33-42. 2004..Such a finding opens the way forward for genetic mapping of the loci responsible for the induction of protective immunity and integration with the genome sequencing efforts...
- Genetic identification of antigens protective against coccidiaD P Blake
Enteric Immunology Group, Institute for Animal Health, Compton, Nr Newbury, Berkshire, UK
Parasite Immunol 28:305-14. 2006..maxima genome linked to escape from a protective immune response. These regions now form the basis of detailed study to identify antigens as candidates for inclusion in future vaccination strategies...
- Parasite genetics and the immune host: a novel strategy in the search for a vaccine against Eimeria sppD P Blake
Institute for Animal Health, Compton, Nr Newbury, Berkshire, RG20 7NN
Br Poult Sci 45:S16-7. 2004
- Modelling host cell availability and the crowding effect in Eimeria infectionsW T Johnston
Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, Newbury, UK
Int J Parasitol 31:1070-81. 2001..Alternative factors such as the influence of the primary immune response to the parasite may also be explored using within-host models and other applications of these models are discussed...
- Mapping the genome of Eimeria tenella: use of a yeast artificial chromosome library as the basis for a physical mapB Hoogendoorn
Institute for Animal Health, Compton, Berkshire, UK
Parasitol Res 84:13-6. 1998..A probe representing the whole of the 1.2-Mb chromosome 2, excised from a preparative gel following pulsed-field gel electrophoresis, revealed between 80 and 100 positive clones...
- Amplified fragment length polymorphism analyses of Eimeria spp.: an improved process for genetic studies of recombinant parasitesD P Blake
Institute for Animal Health, Compton near Newbury, Berkshire RG20 7NN, UK
Parasitol Res 90:473-5. 2003..Our new method should also be useful for genetic analyses of other coccidial parasites and for the recovery of AFLP-quality DNA from other pathogens...
- The influence of immunizing dose size and schedule on immunity to subsequent challenge with antigenically distinct strains of Eimeria maximaDamer P Blake
Enteric Immunology Group, Institute for Animal Health, Compton, Nr Newbury, Berkshire, RG20 7NN, UK
Avian Pathol 34:489-94. 2005..These antigens supplement a separate restricted pool of antigens that are capable of stimulating stronger, but strain-specific, protective immune responses...
- Eimeria maxima: the influence of host genotype on parasite reproduction as revealed by quantitative real-time PCRDamer P Blake
Enteric Immunology Group, Institute for Animal Health, Compton, Nr Newbury, Berkshire RG20 7NN, UK
Int J Parasitol 36:97-105. 2006..Parasite DNA remained detectable up to 20 days post-infection; 11 days after the last oocysts had been detected leaving the host...
- Challenges in the successful control of the avian coccidiaMartin W Shirley
Molecular Parasitology, Institute for Animal Health, Compton, Nr Newbury, Berkshire RG20 7NN, United Kingdom
Vaccine 25:5540-7. 2007..Some past and present methods of controlling avian coccidia are discussed briefly and we describe progress with a novel approach to identify immunoprotective antigens as vaccine candidates...
- Parasite genetics and the immune host: recombination between antigenic types of Eimeria maxima as an entrée to the identification of protective antigensDamer P Blake
Eimerian Genomics Group, Institute for Animal Health, Compton, Nr Newbury, Berkshire RG20 7NN, UK
Mol Biochem Parasitol 138:143-52. 2004..Consideration of the numbers of parasites recombinant for the two traits implicates very few antigen-encoding loci. Our data provide a potential strategy to identify putative antigen-encoding loci in other parasites...
- Antigenic diversity in Eimeria maxima and the influence of host genetics and immunization schedule on cross-protective immunityAdrian L Smith
Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berks RG20 7NN, United Kingdom
Infect Immun 70:2472-9. 2002..These results are discussed in relation to the number of protective antigens presented by complex pathogens and the development of immunoprotective responses in hosts of different genetic backgrounds...
- metaSHARK: software for automated metabolic network prediction from DNA sequence and its application to the genomes of Plasmodium falciparum and Eimeria tenellaJohn W Pinney
Faculty of Biological Sciences, University of Leeds, LS2 9JT, UK
Nucleic Acids Res 33:1399-409. 2005..5x to 7.5x. Lastly, as an example of how metaSHARK can be used to evaluate the genomic evidence for specific metabolic pathways, we present a study of coenzyme A biosynthesis in P.falciparum and E.tenella...