L H Pearl

Summary

Affiliation: Institute of Cancer Research
Country: UK

Publications

  1. ncbi request reprint Structure and function in the uracil-DNA glycosylase superfamily
    L H Pearl
    Section of Structural Biology and CRC DNA Repair Enzyme Group, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, SW3 6JB, London, UK
    Mutat Res 460:165-81. 2000
  2. pmc Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2
    S Ceschini
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 20:6601-11. 2001
  3. pmc Crystal structure and induction mechanism of AmiC-AmiR: a ligand-regulated transcription antitermination complex
    B P O'Hara
    Centre for Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 18:5175-86. 1999
  4. ncbi request reprint Structure and in vivo function of Hsp90
    L H Pearl
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London, SW3 6JB, UK
    Curr Opin Struct Biol 10:46-51. 2000
  5. ncbi request reprint Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2
    J A Hinks
    CR UK DNA Repair Enzyme Group, Institute of Cancer Research, 237 Fulham Road, South Kensington, London SW3 6JB, England
    Acta Crystallogr D Biol Crystallogr 59:1230-3. 2003
  6. ncbi request reprint Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition
    R Dajani
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, United Kingdom
    Cell 105:721-32. 2001
  7. ncbi request reprint Structure, function, and mechanism of the Hsp90 molecular chaperone
    L H Pearl
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Adv Protein Chem 59:157-86. 2001
  8. ncbi request reprint Molecular expression systems for anti-DNA antibodies--2
    S Kumar
    Centre for Rheumatology, Bloomsbury Rheumatology Unit, Department of Medicine, University College London Hospital, London, UK
    Lupus 11:833-42. 2002
  9. pmc Crystal structure of a thwarted mismatch glycosylase DNA repair complex
    T E Barrett
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT
    EMBO J 18:6599-609. 1999
  10. pmc Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis
    T H Tan
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Biochem J 357:373-83. 2001

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Structure and function in the uracil-DNA glycosylase superfamily
    L H Pearl
    Section of Structural Biology and CRC DNA Repair Enzyme Group, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, SW3 6JB, London, UK
    Mutat Res 460:165-81. 2000
    ....
  2. pmc Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2
    S Ceschini
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 20:6601-11. 2001
    ..Detailed analysis of the dimeric structure suggests mechanisms for junction isomerization and communication between the two active sites, and together with site-directed mutagenesis identifies residues involved in catalysis...
  3. pmc Crystal structure and induction mechanism of AmiC-AmiR: a ligand-regulated transcription antitermination complex
    B P O'Hara
    Centre for Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 18:5175-86. 1999
    ..Comparison with the structure of free AmiC reveals the subtle mechanism of ligand-induced release of AmiR...
  4. ncbi request reprint Structure and in vivo function of Hsp90
    L H Pearl
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London, SW3 6JB, UK
    Curr Opin Struct Biol 10:46-51. 2000
    ..Despite this, a clear understanding of how Hsp90 contributes to the folding and/or activation of its client proteins remains some way off...
  5. ncbi request reprint Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2
    J A Hinks
    CR UK DNA Repair Enzyme Group, Institute of Cancer Research, 237 Fulham Road, South Kensington, London SW3 6JB, England
    Acta Crystallogr D Biol Crystallogr 59:1230-3. 2003
    ..04, c = 70.42 A, and tetragonal, P4/m Laue group symmetry, unit-cell parameters a = b = 72.3, c = 91.1 A)...
  6. ncbi request reprint Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition
    R Dajani
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, United Kingdom
    Cell 105:721-32. 2001
    ....
  7. ncbi request reprint Structure, function, and mechanism of the Hsp90 molecular chaperone
    L H Pearl
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Adv Protein Chem 59:157-86. 2001
  8. ncbi request reprint Molecular expression systems for anti-DNA antibodies--2
    S Kumar
    Centre for Rheumatology, Bloomsbury Rheumatology Unit, Department of Medicine, University College London Hospital, London, UK
    Lupus 11:833-42. 2002
    ....
  9. pmc Crystal structure of a thwarted mismatch glycosylase DNA repair complex
    T E Barrett
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT
    EMBO J 18:6599-609. 1999
    ....
  10. pmc Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis
    T H Tan
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Biochem J 357:373-83. 2001
    ..spiralis larvae cultured in vitro at 6 ng/ml (55 ng/mg total secreted protein). In conclusion TsMIF has structural, catalytic and cell-migration-inhibitory properties which indicate that it is partially orthologous to mammalian MIF...
  11. ncbi request reprint Crystal structure of an octameric RuvA-Holliday junction complex
    S M Roe
    Department of Biochemistry and Molecular Biology, University College London, United Kingdom
    Mol Cell 2:361-72. 1998
    ..Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA...
  12. ncbi request reprint Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct
    T E Barrett
    Department of Biochemistry and Molecular Biology, University College London, UK
    Nat Struct Biol 5:697-701. 1998
    ....
  13. ncbi request reprint Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions
    T E Barrett
    Department of Biochemistry and Molecular Biology, University College London, United Kingdom
    Cell 92:117-29. 1998
    ..Details of the MUG structure explain its thymine DNA-glycosylase activity and the specificity for G:U/T mispairs, which derives from direct recognition of guanine on the complementary strand...
  14. ncbi request reprint Oligomerization of the amide sensor protein AmiC by x-ray and neutron scattering and molecular modeling
    D Chamberlain
    Department of Biochemistry, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U K
    Biochemistry 36:8020-9. 1997
    ....
  15. ncbi request reprint Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa
    S A Wilson
    Department of Biochemistry and Molecular Biology, University College London, U K
    J Mol Biol 222:869-71. 1991
    ..Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P4(2)2(1)2 (a = 104.4 A, c = 66.6 A) with one subunit in the asymmetric unit. Crystals diffract beyond 2.8 A...
  16. pmc Antitermination of amidase expression in Pseudomonas aeruginosa is controlled by a novel cytoplasmic amide-binding protein
    S A Wilson
    Biomolecular Structure Group, University College London, UK
    EMBO J 12:3637-42. 1993
    ..Sequence analysis techniques suggest that AmiC is a member of the structural family of periplasmic binding proteins, but has a distinct and novel cytoplasmic role...
  17. ncbi request reprint Expression and crystallization of the yeast Hsp82 chaperone, and preliminary X-ray diffraction studies of the amino-terminal domain
    C Prodromou
    Department of Biochemistry and Molecular Biology, University College London, United Kingdom
    Proteins 25:517-22. 1996
    ..The crystals diffract to 2.8 A resolution, have an orthorhombic space group (P222(1), P2(1)2(1)2, or P2(1)2(1)2(1)) with cell dimensions of a = 45.2 A, b = 115.4 A, and c = 116.9 A, and a solvent content of 58% by volume...
  18. ncbi request reprint Crystallization and preliminary crystallographic analysis of human alanine:glyoxylate aminotransferase and its polymorphic variants
    X Zhang
    Department of Biology, University College London, Gower Street, London WC1E 6BT, England
    Acta Crystallogr D Biol Crystallogr 57:1936-7. 2001
    ..Complete data have been collected for all three AGT variants. The crystals of AGT[P11L] belong to space group P4(1)2(1)2 (or its enantiomorph), with unit-cell parameters a = b = 90.81, c = 142.62 A, and diffract to a resolution of 2.8 A...
  19. ncbi request reprint A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone
    C Prodromou
    Department of Biochemistry and Molecular Biology, University College London, UK
    Nat Struct Biol 4:477-82. 1997
    ..The opposing faces of the beta-sheets in the dimer define a potential peptide-binding cleft, suggesting that the N-domain may serve as a molecular 'clamp' in the binding of ligand proteins to Hsp90...
  20. ncbi request reprint Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability
    C F Aguilar
    Department of Biochemistry and Molecular Biology, Univesity College London, Gower Street, London, WC1E 6BT, UK
    J Mol Biol 271:789-802. 1997
    ..These factors suggest a model for hyperthermostability via resilience rather than rigidity...
  21. ncbi request reprint Structural adaptation to selective pressure for altered ligand specificity in the Pseudomonas aeruginosa amide receptor, amiC
    B P O'Hara
    Department of Biochemistry and Molecular Biology and Joint UCL LICR X Ray Crystallography Laboratory, University College London, Gower Street, London WC1E 6BT, UK
    Protein Eng 13:129-32. 2000
    ..Although the mutation allows butyramide to be recognized as an inducer of amidase expression, the mutation is structurally sub-optimal, and produces a significant decrease in the stability of the mutant protein...
  22. ncbi request reprint The baculovirus Autographa californica nuclear polyhedrosis virus genome includes a papain-like sequence
    N D Rawlings
    Biochemistry Department, Strangeways Research Laboratory, Cambridge, U K
    Biol Chem Hoppe Seyler 373:1211-5. 1992
    ..Irrespective of whether the sequence encodes a functionally active cysteine endopeptidase, this is the first example of a papain-related sequence in a viral genome...