G Dougan

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. pmc Intracellular adhesion molecule 1 plays a key role in acquired immunity to salmonellosis
    Simon Clare
    Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Level 1, Flowers Building, Imperial College, South Kensington, London SW7 2AZ, United Kingdom
    Infect Immun 71:5881-91. 2003
  2. ncbi request reprint The Escherichia coli gene pool
    G Dougan
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, SW7 2AZ, London, UK
    Curr Opin Microbiol 4:90-4. 2001
  3. pmc The immune responses to bacterial antigens encountered in vivo at mucosal surfaces
    G Dougan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    Philos Trans R Soc Lond B Biol Sci 355:705-12. 2000
  4. ncbi request reprint Chronic bacterial infections: living with unwanted guests
    Douglas Young
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Nat Immunol 3:1026-32. 2002
  5. pmc Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1
    S J Dunstan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom
    J Infect Dis 183:1156-60. 2001
  6. pmc Intimin-specific immune responses prevent bacterial colonization by the attaching-effacing pathogen Citrobacter rodentium
    M Ghaem-Maghami
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London SW7 2AZ, United Kingdom
    Infect Immun 69:5597-605. 2001
  7. ncbi request reprint Site-directed mutagenesis of intimin alpha modulates intimin-mediated tissue tropism and host specificity
    S Reece
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Mol Microbiol 40:86-98. 2001
  8. ncbi request reprint Intimin and the host cell--is it bound to end in Tir(s)?
    G Frankel
    Centre for Molecular Microbiology and Infection, Dept of Biochemistry, Imperial College of Science, Technology and Medicine, SW7 2AZ, London, UK
    Trends Microbiol 9:214-8. 2001
  9. ncbi request reprint Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells
    E L Hartland
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    Mol Microbiol 32:151-8. 1999
  10. ncbi request reprint Structure of the cell-adhesion fragment of intimin from enteropathogenic Escherichia coli
    G Kelly
    Department of Biochemistry and Centre for Structural Biology, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
    Nat Struct Biol 6:313-8. 1999

Collaborators

Detail Information

Publications102 found, 100 shown here

  1. pmc Intracellular adhesion molecule 1 plays a key role in acquired immunity to salmonellosis
    Simon Clare
    Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Level 1, Flowers Building, Imperial College, South Kensington, London SW7 2AZ, United Kingdom
    Infect Immun 71:5881-91. 2003
    ..In this study we show that ICAM-1 plays a critical role during rechallenge of immunized mice with virulent S. enterica serovar Typhimurium...
  2. ncbi request reprint The Escherichia coli gene pool
    G Dougan
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, SW7 2AZ, London, UK
    Curr Opin Microbiol 4:90-4. 2001
    ..coli to adapt to new environments. These additional genes are often clustered, have related functions (for example, virulence-associated genes in pathogenicity islands) and may be integrated at specific sites on the E. coli chromosome...
  3. pmc The immune responses to bacterial antigens encountered in vivo at mucosal surfaces
    G Dougan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    Philos Trans R Soc Lond B Biol Sci 355:705-12. 2000
    ..Studies on mucosal immunogens may facilitate the identification of common characteristics that contribute to mucosal immunogenicity and aid the development of novel, non-living mucosal vaccines and immunostimulators...
  4. ncbi request reprint Chronic bacterial infections: living with unwanted guests
    Douglas Young
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Nat Immunol 3:1026-32. 2002
    ..Bacteria appear to adapt their immediate environment to favor survival and may hijack essential immunoregulatory mechanisms designed to minimize immune pathology or the inappropriate activation of immune effectors...
  5. pmc Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1
    S J Dunstan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom
    J Infect Dis 183:1156-60. 2001
    ..No allelic association was identified between the NRAMP1 alleles and typhoid fever susceptibility. In addition, neither homozygotes nor heterozygotes for any NRAMP1 variants were at increased risk of typhoid fever...
  6. pmc Intimin-specific immune responses prevent bacterial colonization by the attaching-effacing pathogen Citrobacter rodentium
    M Ghaem-Maghami
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London SW7 2AZ, United Kingdom
    Infect Immun 69:5597-605. 2001
    ..These results show that anti-intimin immune responses can modulate the outcome of a C. rodentium infection and support the use of intimin as a component of a type-specific EPEC or EHEC vaccine...
  7. ncbi request reprint Site-directed mutagenesis of intimin alpha modulates intimin-mediated tissue tropism and host specificity
    S Reece
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Mol Microbiol 40:86-98. 2001
    ..This study provides the first clues of the molecular basis of how intimin mediates tissue tropism and host specificity...
  8. ncbi request reprint Intimin and the host cell--is it bound to end in Tir(s)?
    G Frankel
    Centre for Molecular Microbiology and Infection, Dept of Biochemistry, Imperial College of Science, Technology and Medicine, SW7 2AZ, London, UK
    Trends Microbiol 9:214-8. 2001
    ..Recent data support the hypothesis that the formation of a stable intimin-Tir relationship is the consequence of intimin protein interactions involving both host and bacterial components...
  9. ncbi request reprint Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells
    E L Hartland
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    Mol Microbiol 32:151-8. 1999
    ..These data provide strong evidence that intimin interacts not only with Tir but also in a lectin-like manner with a host cell intimin receptor...
  10. ncbi request reprint Structure of the cell-adhesion fragment of intimin from enteropathogenic Escherichia coli
    G Kelly
    Department of Biochemistry and Centre for Structural Biology, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
    Nat Struct Biol 6:313-8. 1999
    ..These findings also imply that carbohydrate recognition may be important in intimin-mediated cell adhesion...
  11. ncbi request reprint Subtyping intimin genes from enteropathogenic Escherichia coli associated with outbreaks and sporadic cases in the United Kingdom and Eire
    C Jenkins
    Laboratory of Enteric Pathogens, Central Public Health Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Avenue, NW9 5HT, London, UK
    Mol Cell Probes 17:149-56. 2003
    ..The beta-intimin gene was the most frequently detected sub-type in both the outbreak and sporadic strains...
  12. ncbi request reprint Mucosal delivery of a respiratory syncytial virus CTL peptide with enterotoxin-based adjuvants elicits protective, immunopathogenic, and immunoregulatory antiviral CD8+ T cell responses
    C P Simmons
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, United Kingdom
    J Immunol 166:1106-13. 2001
    ..These studies emphasize that protective and immunoregulatory CD8(+) CTL responses can be mucosally elicited using enterotoxin-based mucosal adjuvants but that resistance against viral infection may be accompanied by enhanced disease...
  13. ncbi request reprint Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques
    C Jenkins
    Laboratory of Enteric Pathogens, Health Protection Agency, 61 Colindale Avenue, London, NW9 5HT, UK
    Lett Appl Microbiol 37:207-12. 2003
    ....
  14. pmc Structural basis for recognition of the translocated intimin receptor (Tir) by intimin from enteropathogenic Escherichia coli
    M Batchelor
    Department of Biochemistry and Centre for Structural Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    EMBO J 19:2452-64. 2000
    ..The interpretation of NMR-titration and mutagenesis data has enabled us to identify, for the first time, the binding site for Tir, which is located at the extremity of the Int190 moiety...
  15. pmc Intimin from enteropathogenic Escherichia coli restores murine virulence to a Citrobacter rodentium eaeA mutant: induction of an immunoglobulin A response to intimin and EspB
    G Frankel
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom
    Infect Immun 64:5315-25. 1996
    ..These results show that C. rodentium infection provides a relevant, simple, and economic model to investigate the role of EPEC proteins in the formation of A/E lesions in vivo and in intestinal disease...
  16. pmc Interleukin 18 contributes to host resistance and gamma interferon production in mice infected with virulent Salmonella typhimurium
    P Mastroeni
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London SW7 2AZ, United Kingdom
    Infect Immun 67:478-83. 1999
    ..Thus, IL-18 is a key factor in early host resistance to Salmonella and probably acts via IFN-gamma...
  17. pmc Serology of typhoid fever in an area of endemicity and its relevance to diagnosis
    D House
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, South Kensington, London SW7 2AZ, United Kingdom
    J Clin Microbiol 39:1002-7. 2001
    ..007). These tests could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever but are culture negative or in regions where bacterial culturing facilities are not available...
  18. ncbi request reprint Immunomodulation using bacterial enterotoxins
    C P Simmons
    Department of Biochemistry, Imperial College of Science Technology and Medicine, South Kensington, London SW7 2AZ, UK
    Scand J Immunol 53:218-26. 2001
    ..This review summarizes some of the structural and biological characteristics of these toxins and provides examples of how these properties have been exploited for tolerance induction and mucosal vaccine development...
  19. ncbi request reprint Understanding mucosal responsiveness: lessons from enteric bacterial pathogens
    C P Simmons
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Semin Immunol 13:201-9. 2001
    ..This review will summarize some of the lessons learnt from studies of antigens derived from enteric bacterial pathogens and discuss how the gastrointestinal epithelia can 'fight back' when it encounters pathogens...
  20. pmc Acquisition of virulence-associated factors by the enteric pathogens Escherichia coli and Salmonella enterica
    J Wain
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, South Kensington, London W7 2AZ, UK
    Philos Trans R Soc Lond B Biol Sci 356:1027-34. 2001
    ..We show how acquisition of DNA at specific sites on the chromosome has contributed to increased genetic variation and virulence of these two genera of the Enterobacteriaceae...
  21. pmc Characterization of candidate live oral Salmonella typhi vaccine strains harboring defined mutations in aroA, aroC, and htrA
    D C Lowe
    Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge CB2 4AT, Imperial College of Science, Technology and Medicine, London SW7 2AY, United Kingdom
    Infect Immun 67:700-7. 1999
    ....
  22. pmc Identification of immunodominant regions within the C-terminal cell binding domain of intimin alpha and intimin beta from enteropathogenic Escherichia coli
    J Adu-Bobie
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom
    Infect Immun 66:5643-9. 1998
    ..The results with the human colostra revealed one reactivity pattern against the Int280alpha fragments but two different reactivity patterns against the Int280beta domain...
  23. ncbi request reprint Characterisation of Candida albicans infections of haematogenous and mucosal origin in mice lacking the interferon gamma receptor protein
    L P Londono
    Biochemistry Department, Imperial College of Science, Technology and Medicine, Exhibition Road, London, UK
    FEMS Immunol Med Microbiol 27:117-25. 2000
    ..albicans multiplication during the early stages of infection, as well as in preventing the development of C. albicans-associated abscesses. Activation of macrophages by IFNgamma might be pivotal in mediating this role...
  24. ncbi request reprint MHC class I-restricted cytotoxic lymphocyte responses induced by enterotoxin-based mucosal adjuvants
    C P Simmons
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, United Kingdom
    J Immunol 163:6502-10. 1999
    ..The results highlight the versatility of toxin-based adjuvants and suggest that LT potentiates CTL responses independently of IL-12 and IFN-gamma and probably by a mechanism unrelated to cross-priming...
  25. pmc Evaluation of Salmonella typhimurium mutants in a model of experimental gastroenteritis
    P Everest
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom
    Infect Immun 67:2815-21. 1999
    ..typhimurium aroA strains, whereas S. typhimurium invA-infected loops showed no detectable damage. This suggests that villus damage most likely contributes to fluid accumulation within the loop...
  26. ncbi request reprint An eight-month study of a population of verocytotoxigenic Escherichia coli (VTEC) in a Scottish cattle herd
    C Jenkins
    Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK
    J Appl Microbiol 93:944-53. 2002
    ..Characteristics of these strains were examined to allow comparisons with strains of VTEC associated with human infection...
  27. ncbi request reprint Typhoid fever: pathogenesis and disease
    D House
    Centre for Molecular Microbiology and Infection, Imperial College of Science Technology and Medicine, London, UK
    Curr Opin Infect Dis 14:573-8. 2001
    ..In addition, we attempt to put current salmonella research into the context of typhoid fever...
  28. ncbi request reprint Edwardsiella tarda induces plasma membrane ruffles on infection of HEp-2 cells
    A D Phillips
    University Department of Paediatric Gastroenterology, Royal Free Hospital, London, UK
    FEMS Microbiol Lett 161:317-23. 1998
    ..Only one of the E. tarda strains was seen residing within the host cell remains. The results indicate that E. tarda-induced membrane ruffles may involve a distinct mechanism of bacterial pathogenesis...
  29. ncbi request reprint A vaccination strategy incorporating DNA priming and mucosal boosting using tetanus toxin fragment C (TetC)
    R Stratford
    Centre for Molecular Microbiology and Infection, Department of Biochemistry and Biology, Imperial College of Science, Technology and Medicine, Exhibition Road, London SW7 2AY, UK
    Vaccine 20:516-25. 2001
    ..The immunomodulatory effect of pcDNA3/tetC appeared to be antigen specific as mucosal boosting with an unrelated antigen (pertactin) revealed no significant modulation in terms of the anti-pertactin immune response...
  30. pmc Characterization of defined ompR mutants of Salmonella typhi: ompR is involved in the regulation of Vi polysaccharide expression
    D Pickard
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom
    Infect Immun 62:3984-93. 1994
    ..typhi and that one of the environmental signals for this regulation may be osmolarity...
  31. ncbi request reprint Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements
    G Frankel
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK
    Mol Microbiol 30:911-21. 1998
    ..Major breakthroughs and developments in the genetic basis of A/E lesion formation, signal transduction, protein translocation, host cell receptors and intestinal colonization are highlighted in this review...
  32. pmc Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7
    R J Fitzhenry
    Centre for Paediatric Gastroenterology, Department of Paediatrics and Child Health, Royal Free Hospital, London, UK
    Gut 50:180-5. 2002
    ..EHEC (O157:H7) colonises human intestinal explants although adhesion is restricted to the follicle associated epithelium of Peyer's patches. This phenotype is also observed with EPEC O127:H6 engineered to express EHEC intimin gamma...
  33. ncbi request reprint Bacterial copper- and zinc-cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis
    J L Farrant
    Department of Paediatrics, Imperial College School of Medicine at St Mary s, Norfolk Place, London, UK
    Mol Microbiol 25:785-96. 1997
    ....
  34. ncbi request reprint Construction and characterisation of a Yersinia enterocolitica O:8 ompR mutant
    N Dorrell
    Department of Medical Microbiology, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    FEMS Microbiol Lett 165:145-51. 1998
    ..The mutant was shown to have increased sensitivity to high osmolarity, high temperature and low pH stresses in vitro. In the murine yersiniosis model, the mutant was attenuated and offered partial protection against wild-type challenge...
  35. ncbi request reprint Protection of chickens against experimental fowl typhoid using a nuoG mutant of Salmonella serotype Gallinarum
    L Zhang-Barber
    Institute for Animal Health, Compton, Newbury Berkshire, UK
    Vaccine 16:899-903. 1998
    ..Using a single oral immunization with live bacteria SG9NGK reduced mortality in 2-week-old chickens following challenge with virulent S. Gallinarum from 75% to less than 8%...
  36. pmc Interleukin-12 is required for control of the growth of attenuated aromatic-compound-dependent salmonellae in BALB/c mice: role of gamma interferon and macrophage activation
    P Mastroeni
    School of Microbiological, Immunological and Virological Sciences, Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, United Kingdom
    Infect Immun 66:4767-76. 1998
    ..Administration of recombinant IFN-gamma to anti-IL-12-treated mice was able to restore host resistance, granuloma formation, and expression of major histocompatibility complex class II antigens in F4/80(+) and CD11b+ spleen cells...
  37. ncbi request reprint Homocysteine modification of HLA antigens and its immunological consequences
    X M Gao
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, GB
    Eur J Immunol 26:1443-50. 1996
    ....
  38. ncbi request reprint Universal DNA primers amplify bacterial DNA from human fetal membranes and link Fusobacterium nucleatum with prolonged preterm membrane rupture
    R J Cahill
    Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Flowers Building, London, UK
    Mol Hum Reprod 11:761-6. 2005
    ..nucleatum. Bacteria from fetal membranes may be identified by molecular and bioinformatic methods. Further work is warranted to investigate the apparent linkage between F. nucleatum, fetal membrane rupture and preterm delivery...
  39. ncbi request reprint The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases
    T Garbe
    MRC Tuberculosis and Related Infections Unit, Hammersmith Hospital, London, UK
    Mol Gen Genet 228:385-92. 1991
    ..Deletion of genes encoding shikimate pathway enzymes represents a possible approach to generation of rationally attenuated strains of M. tuberculosis for use as live vaccines...
  40. ncbi request reprint Salmonella typhimurium infections in mice deficient in interleukin-4 production: role of IL-4 in infection-associated pathology
    P Everest
    Department of Biochemistry, Imperial College of Science Technology, and Medicine, London, United Kingdom
    J Immunol 159:1820-7. 1997
    ..The immune response in both IL-4(-/-) and IL-4(+/+) mice was of the Th1-type. Viable salmonella bacteria could be found associated with abscesses. Both IL-4(-/-) and IL-4(+/+) mice were resistant to killing by S. typhimurium aroA...
  41. pmc Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions
    M A Chambers
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom
    J Virol 70:4045-52. 1996
    ..Thus, HBc particles may provide a simple, general system for exploring the interactions of specific peptide sequences with cellular receptors...
  42. ncbi request reprint Genes of the class II and class III major histocompatibility complex are associated with typhoid fever in Vietnam
    S J Dunstan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, SW7 2AZ, United Kingdom
    J Infect Dis 183:261-268. 2001
    ..DRB1*04) or predisposed individuals to typhoid fever (TNFA*2 [-308].DRB1*0301) were determined. This report identifies a genetic association in humans between typhoid fever and MHC class II and III genes...
  43. pmc Interaction of Salmonella enterica serovar Typhi with cultured epithelial cells: roles of surface structures in adhesion and invasion
    Anne Bishop
    The Centre for Molecular Microbiology and Infection, Faculty of Life Sciences, Division of Molecular and Cell Biology, Imperial College London, London SW7 2AZ, UK
    Microbiology 154:1914-26. 2008
    ..Mutation of the staA, tcfA or pilS genes did not affect invasion or adhesion in either the presence or the absence of Vi capsule...
  44. pmc Use of paired serum samples for serodiagnosis of typhoid fever
    Deborah House
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, South Kensington, UK
    J Clin Microbiol 43:4889-90. 2005
    ....
  45. pmc Detection of Vi-negative Salmonella enterica serovar typhi in the peripheral blood of patients with typhoid fever in the Faisalabad region of Pakistan
    Stephen Baker
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    J Clin Microbiol 43:4418-25. 2005
    ..The method described here can be used to monitor the incidence of Vi-negative serovar Typhi in regions where the Vi vaccine is used...
  46. pmc Vi antigen expression in Salmonella enterica serovar Typhi clinical isolates from Pakistan
    John Wain
    Investigative Sciences, Centre for Molecular Microbiology and Infection, Imperial College, London, United Kingdom
    J Clin Microbiol 43:1158-65. 2005
    ....
  47. pmc High-throughput genotyping of Salmonella enterica serovar Typhi allowing geographical assignment of haplotypes and pathotypes within an urban District of Jakarta, Indonesia
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    J Clin Microbiol 46:1741-6. 2008
    ..The study also provides insight into the evolution of serovar Typhi and demonstrates the value of a molecular epidemiological technique that is exchangeable, that is internet friendly, and that has global utility...
  48. pmc Optimization of Salmonella enterica serovar typhi DeltaaroC DeltassaV derivatives as vehicles for delivering heterologous antigens by chromosomal integration and in vivo inducible promoters
    Richard Stratford
    Microscience Ltd, 540 545 Eskdale Rd, Winnersh Triangle, Wokingham, Berkshire RG41 5TU, United Kingdom
    Infect Immun 73:362-8. 2005
    ..Immunization of mice with these S. enterica serovar Typhi ZH9 derivatives stimulated potent antigen-specific serum immunoglobulin G responses to the heterologous antigens...
  49. ncbi request reprint Expression of heterologous antigens in Salmonella Typhimurium vaccine vectors using the in vivo-inducible, SPI-2 promoter, ssaG
    Nicola D McKelvie
    Microscience Limited, 540 545 Eskdale Road, Winnersh Triangle, Wokingham, Berkshire RG41 5TU, UK
    Vaccine 22:3243-55. 2004
    ..Expression of LT-B by these Salmonella derivatives was detected at significant levels after intra-macrophage survival and mice immunised with these derivatives mounted marked anti-LT-B humoral antibody responses...
  50. ncbi request reprint Live Salmonella enterica serovar Typhimurium (S. Typhimurium) elicit dendritic cell responses that differ from those induced by killed S. Typhimurium
    Mari Norimatsu
    Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK
    Vet Immunol Immunopathol 98:193-201. 2004
    ..These data suggest that blood-monocyte-derived DC may follow distinct maturation pathways following exposure to live or killed bacteria. These differences are likely to have consequences for the priming of the adaptive immune responses...
  51. pmc High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi
    Kathryn E Holt
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Genet 40:987-93. 2008
    ..The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers...
  52. pmc Evolutionary history of Salmonella typhi
    Philippe Roumagnac
    Max Planck Institut für Infektionsbiologie, Department of Molecular Biology, Chariteplatz 1, 10117 Berlin, Germany
    Science 314:1301-4. 2006
    ..Neutral evolution in Typhi appears to reflect the asymptomatic carrier state, and adaptive evolution depends on the rapid transmission of phenotypic changes through acute infections...
  53. pmc A linear plasmid truncation induces unidirectional flagellar phase change in H:z66 positive Salmonella Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Mol Microbiol 66:1207-18. 2007
    ..These data demonstrate that, like some Streptomyces linear elements, at least one of the terminal inverted repeats of pBSSB1 is non-essential, but that a palindromic repeat sequence may be necessary for replication...
  54. pmc Antimicrobial drug resistance of Salmonella enterica serovar typhi in asia and molecular mechanism of reduced susceptibility to the fluoroquinolones
    Tran Thuy Chau
    Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Ho Chi Minh City, Vietnam
    Antimicrob Agents Chemother 51:4315-23. 2007
    ..The 8-methoxy fluoroquinolone gatifloxacin showed rapid killing of serovar Typhi harboring both the single- and double-amino-acid substitutions...
  55. pmc Prophage sequences defining hot spots of genome variation in Salmonella enterica serovar Typhimurium can be used to discriminate between field isolates
    Fiona J Cooke
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Clin Microbiol 45:2590-8. 2007
    ..These multiplex PCR assays, based on prophage-like elements and Salmonella genomic island 1, provide a simple method for identifying new variants of S. enterica serovar Typhimurium in the field...
  56. pmc The neglected role of antibody in protection against bacteremia caused by nontyphoidal strains of Salmonella in African children
    Calman A MacLennan
    Malawi Liverpool Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre, Malawi
    J Clin Invest 118:1553-62. 2008
    ..This finding and the age-incidence of NTS bacteremia suggest that antibody protects against NTS bacteremia and support the development of vaccines against NTS that induce protective antibody...
  57. pmc Mobilization of the incQ plasmid R300B with a chromosomal conjugation system in Salmonella enterica serovar typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    J Bacteriol 190:4084-7. 2008
    ..Evidence suggests that SPI-7 is susceptible to spontaneous circularization, loss, and transposition. Here, we demonstrate that a region within SPI-7 has the ability to mobilize the small incQ plasmid R300B...
  58. pmc A novel linear plasmid mediates flagellar variation in Salmonella Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    PLoS Pathog 3:e59. 2007
    ..The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage-related linear plasmid to be described in the Enterobacteriaceae...
  59. pmc Requirement of bic/microRNA-155 for normal immune function
    Antony Rodriguez
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 316:608-11. 2007
    ..Our work suggests that bic/microRNA-155 plays a key role in the homeostasis and function of the immune system...
  60. pmc Multidrug-resistant Salmonella enterica serovar paratyphi A harbors IncHI1 plasmids similar to those found in serovar typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    J Bacteriol 189:4257-64. 2007
    ..Our data show that these serovar Typhi and Paratyphi A IncHI1 plasmids share highly conserved core DNA and have acquired similar mobile elements encoding antibiotic resistance genes in past decades...
  61. pmc Candidate live, attenuated Salmonella enterica serotype Typhimurium vaccines with reduced fecal shedding are immunogenic and effective oral vaccines
    Moataz Abd El Ghany
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Infect Immun 75:1835-42. 2007
    ..Further, immunization with serotype Typhimurium aroA candidate vaccines harboring shdA and misL afforded complete protection against challenge with a virulent strain of serotype Typhimurium...
  62. ncbi request reprint Development of attenuated Salmonella strains that express heterologous antigens
    Frances Bowe
    Centre for Molecular Microbiology and Immunology, Department of Biological Sciences, Imperial College, London, United Kingdom
    Methods Mol Med 87:83-100. 2003
  63. pmc Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro
    Mari Norimatsu
    Institute for Animal Health, Compton, Newbury, UK
    Immunology 108:55-61. 2003
    ..typhimurium and this could have important implications for the development of the immune response...
  64. ncbi request reprint Typhoid fever
    Christopher M Parry
    Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom
    N Engl J Med 347:1770-82. 2002
  65. ncbi request reprint A clinical, microbiological, and pathological study of intestinal perforation associated with typhoid fever
    Quoc Chanh Nguyen
    Dong Thap Provincial Hospital, Dong Thap, Ho Chi Minh City, Vietnam
    Clin Infect Dis 39:61-7. 2004
    ..Immunohistochemical results showed that the predominant infiltrating cell types at the site of perforation were CD68+ leukocytes (macrophages) or CD3+ leukocytes (T lymphocytes)...
  66. ncbi request reprint Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2
    Claire Kidgell
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK
    Plasmid 47:159-71. 2002
    ..typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world...
  67. ncbi request reprint Cytokine release by lipopolysaccharide-stimulated whole blood from patients with typhoid fever
    Deborah House
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology, and Medicine, London, United Kingdom
    J Infect Dis 186:240-5. 2002
    ..In acute typhoid fever, the ability of peripheral blood leukocytes to release proinflammatory cytokines in response to an inflammatory stimulus is depressed, and this may contribute to delayed recovery following antibiotic treatment...
  68. pmc Characterization of Salmonella enterica derivatives harboring defined aroC and Salmonella pathogenicity island 2 type III secretion system (ssaV) mutations by immunization of healthy volunteers
    Zoe Hindle
    Microscience, Wokingham Berkshire RG41 5TU, United Kingdom
    Infect Immun 70:3457-67. 2002
    ..These data indicate that mutations in type III secretion systems may provide a route to the development of live vaccines in humans and highlight significant differences in the potential use of serovars Typhimurium and Typhi...
  69. ncbi request reprint Unlocking the genome of the human typhoid bacillus
    John Wain
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, UK
    Lancet Infect Dis 2:163-70. 2002
    ..We have new insights into the mechanisms underpinning the human host specificity of S typhi, and have exploitable new routes to improved diagnostics and a better understanding of the epidemiology of the disease...
  70. ncbi request reprint Salmonella typhi and S typhimurium derivatives harbouring deletions in aromatic biosynthesis and Salmonella Pathogenicity Island-2 (SPI-2) genes as vaccines and vectors
    Shahid A Khan
    Microscience Limited, 545 Eskdale Road, Winnersh Triangle, Wokingham, Berkshire RG41 5TU, UK
    Vaccine 21:538-48. 2003
    ..We conclude that deltaaroC deltassaV strains of Salmonella are highly attenuated and are promising candidates both as human typhoid vaccines and as vaccine vectors for the delivery of heterologous antigens...
  71. pmc Molecular analysis of incHI1 antimicrobial resistance plasmids from Salmonella serovar Typhi strains associated with typhoid fever
    John Wain
    Wellcome Trust Clinical Research Unit, Department of Biological Sciences, Imperial College, London
    Antimicrob Agents Chemother 47:2732-9. 2003
    ..In 1996 a single RFLP type, RFLP7, was found to carry the dfrA7 and sul-1 genes, which were not present on R27 or pHCM1. This plasmid type appears to have a selective advantage over other plasmids with the same resistance phenotype...
  72. pmc Central role for B lymphocytes and CD4+ T cells in immunity to infection by the attaching and effacing pathogen Citrobacter rodentium
    Cameron P Simmons
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, South Kensington, London SW6 1SJ, United Kingdom
    Infect Immun 71:5077-86. 2003
    ..rodentium infection but that the mechanism through which this occurs requires further investigation. These data are relevant to understanding immunity to enteric attaching and effacing bacterial pathogens of humans...
  73. ncbi request reprint Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium, in mice lacking IL-12 or IFN-gamma
    Cameron P Simmons
    Department of Biochemistry, Center for Molecular Microbiology and Infection, Imperial College, South Kensington, London, United Kingdom
    J Immunol 168:1804-12. 2002
    ..These studies are among the first to examine mechanisms of host resistance to an attaching-effacing pathogen and show an important role for IL-12 and IFN-gamma in limiting bacterial infection of the colonic epithelium...
  74. ncbi request reprint Helicobacter pylori has stimulatory effects on naive T cells
    Anna Maria Malfitano
    Department of Infectious Diseases and Microbiology, Hammersmith Campus, Imperial College London, London, UK
    Helicobacter 11:21-30. 2006
    ..Unexpectedly, T cells from apparently uninfected individuals respond to H. pylori antigen by proliferating. Also, the T-cell proliferative response appears to be less in infected compared with uninfected individuals...
  75. pmc A TNF region haplotype offers protection from typhoid fever in Vietnamese patients
    Sarah J Dunstan
    Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Quan 5, District 5, Ho Chi Minh City, Vietnam
    Hum Genet 122:51-61. 2007
    ..Finally, individuals that carry the typhoid fever protective haplotype *12122*1111 also produce a relatively low TNF-alpha response to LPS...
  76. ncbi request reprint Host/pathogen interactions at mucosal surfaces: immune consequences
    Simon Clare
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London, UK
    Res Microbiol 153:455-9. 2002
    ..Infection models involving microorganisms such as Citrobacter rodentium can also be used to investigate the consequences of mucosal colonisation that lead to immune disfunction...
  77. pmc Quorum sensing has an unexpected role in virulence in the model pathogen Citrobacter rodentium
    Sarah J Coulthurst
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
    EMBO Rep 8:698-703. 2007
    ..Further study of QS in Citrobacter should provide new insights into AE pathogenesis. As the croIR locus might have been horizontally acquired, AHL QS might exist in some strains of pathogenic E. coli...
  78. ncbi request reprint The genome of Salmonella enterica serovar Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    Clin Infect Dis 45:S29-33. 2007
    ..Evidence suggests that, as well as undergoing gene degradation, S. Typhi has also recently acquired genes, such as those encoding the Vi antigen, by horizontal transfer events...
  79. pmc Salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota
    Bärbel Stecher
    Institute of Microbiology, Swiss Institute of Technology Zurich, Zurich, Switzerland
    PLoS Biol 5:2177-89. 2007
    ..Triggering the host's immune defence can shift the balance between the protective microbiota and the pathogen in favour of the pathogen...
  80. pmc Boosting of cellular immunity against Mycobacterium tuberculosis and modulation of skin cytokine responses in healthy human volunteers by Mycobacterium bovis BCG substrain Moreau Rio de Janeiro oral vaccine
    Catherine A Cosgrove
    CMM Infectious Diseases, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, United Kingdom
    Infect Immun 74:2449-52. 2006
    ..Oral BCG offers the possibility of a needle-free tuberculosis vaccine and of boosting the protective immunity from intradermal tuberculosis vaccines...
  81. pmc Increased susceptibility of C1q-deficient mice to Salmonella enterica serovar Typhimurium infection
    Joanna Warren
    Rheumatology Section, Division of Medicine, Department of Biochemistry, Imperial College School of Medicine London, University of Cambridge, Cambridge, United Kingdom
    Infect Immun 70:551-7. 2002
    ..The data presented here suggest the possibility of novel pathways by which C1q may modulate the pathogenesis of infectious diseases caused by intracellular pathogens...
  82. ncbi request reprint Cell attachment properties and infectivity of host-adapted and environmentally adapted Citrobacter rodentium
    Anne L Bishop
    Division of Cell and Molecular Biology, Flowers Building, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Microbes Infect 9:1316-24. 2007
    ..rodentium cultured in laboratory media. Thus, the 'hyper-infectious' host-adapted state, allowing efficient transmission and colonisation of naive hosts, is transient in nature and gradually lost after shedding into the environment...
  83. pmc Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries
    Seon Young Choi
    1International Vaccine Institute, San 4 8 Bongcheon 7 Dong, Kwanak gu, Seoul 151 818, Republic of Korea
    J Med Microbiol 56:1460-6. 2007
    ..S. flexneri serotypes 1-5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established...
  84. pmc Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1
    Derek Pickard
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Bacteriol 190:2580-7. 2008
    ..The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity...
  85. pmc Genomic comparison of Salmonella enterica serovars and Salmonella bongori by use of an S. enterica serovar typhimurium DNA microarray
    Kaman Chan
    Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305 5124, USA
    J Bacteriol 185:553-63. 2003
    ....
  86. pmc CD4+CD25+ T(R) cells suppress innate immune pathology through cytokine-dependent mechanisms
    Kevin J Maloy
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom
    J Exp Med 197:111-9. 2003
    ..Thus, CD4(+)CD25(+) T(R) cells do not only suppress adaptive T cell responses, but are also able to control pathology mediated by innate immune mechanisms...
  87. pmc Mucosal vaccination against serogroup B meningococci: induction of bactericidal antibodies and cellular immunity following intranasal immunization with NadA of Neisseria meningitidis and mutants of Escherichia coli heat-labile enterotoxin
    Frances Bowe
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London, UK
    Infect Immun 72:4052-60. 2004
    ....
  88. ncbi request reprint Salmonella enterica serovar Typhimurium interaction with dendritic cells: impact of the sifA gene
    Liljana Petrovska
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Cell Microbiol 6:1071-84. 2004
    ..Mice infected with the S. Typhimurium aroC sifA expressing ovalbumin did not develop detectably enhanced levels of cytotoxic T cell or interferon-gamma production compared to S. Typhimurium aroC derivatives...
  89. pmc Composition, acquisition, and distribution of the Vi exopolysaccharide-encoding Salmonella enterica pathogenicity island SPI-7
    Derek Pickard
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Armstrong Road, London SW7 2AZ, UK
    J Bacteriol 185:5055-65. 2003
    ..citri. This analysis suggested that SPI-7 may be a mobile element, such as a conjugative transposon or an integrated plasmid remnant...
  90. ncbi request reprint Live bacteria as the basis for immunotherapies against cancer
    Jose A Chabalgoity
    Department of Biochemistry, Instituto de Higiene, Facultad de Medicina, Montevideo, Uruguay
    Expert Rev Vaccines 1:495-505. 2002
    ..In this paper, we will review the current state of such applications and highlight some of the directions that the field may take...
  91. pmc Analysis of the hypervariable region of the Salmonella enterica genome associated with tRNA(leuX)
    Anne L Bishop
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Bacteriol 187:2469-82. 2005
    ..coli, suggesting that these mobile genetic elements have played a major role in driving the variability of this region...
  92. ncbi request reprint Cancer immunotherapy based on killing of Salmonella-infected tumor cells
    Francesca Avogadri
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Cancer Res 65:3920-7. 2005
    ..This is effective in retarding the growth of untreated established distant tumors and in protecting the mice from subsequent tumor challenges...
  93. ncbi request reprint Salmonella typhi, the causative agent of typhoid fever, is approximately 50,000 years old
    Claire Kidgell
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, The Flowers Building, Exhibition Road, South Kensington, London SW7 2AY, UK
    Infect Genet Evol 2:39-45. 2002
    ..typhi existed 15,000-150,000 years ago, during the human hunter-gatherer phase and prior to the development of agriculture and the domestication of animals...
  94. ncbi request reprint Host defences to Citrobacter rodentium
    Thomas T MacDonald
    Division of Infection, Inflammation and Repair, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK
    Int J Med Microbiol 293:87-93. 2003
    ..Infection elicits mucosal inflammation with similarities to inflammatory bowel disease, and so it is a readily accessible model to investigate the relationship between inflammation and anti-bacterial immunity in the gut...
  95. pmc Antibodies to the iron uptake ABC transporter lipoproteins PiaA and PiuA promote opsonophagocytosis of Streptococcus pneumoniae
    Maha Jomaa
    Centre for Biological Sciences, Imperial College London, UK
    Infect Immun 73:6852-9. 2005
    ..pneumoniae by the human neutrophil cell line HL60. Hence, vaccination with PiaA and PiuA protects against S. pneumoniae infection by inducing antibodies that promote bacterial opsonophagocytosis rather than inhibiting iron transport...
  96. pmc Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, College Station 77843 1114, USA
    Infect Immun 71:629-40. 2003
    ..These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice...
  97. ncbi request reprint Evaluation of a novel Vi conjugate vaccine in a murine model of salmonellosis
    Christine Hale
    The Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK
    Vaccine 24:4312-20. 2006
    ..typhimurium). In contrast, Vi vaccination did not confer protection against oral challenge with virulent Vi-positive S. typhimurium or S. dublin...
  98. ncbi request reprint The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
    Mohammed Sebaihia
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Genet 38:779-86. 2006
    ..The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism...
  99. ncbi request reprint How bacteria and their products provide clues to vaccine and adjuvant development
    Gordon Dougan
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Vaccine 24:S2-13-9. 2006
    ..New knowledge in this field is beginning to explain how vaccine adjuvants work and will facilitate the future development of novel adjuvants and vaccines...
  100. ncbi request reprint Mucosal immunisation of murine neonates using whole cell and acellular Pertussis vaccines
    Christine Hale
    Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Vaccine 22:3595-602. 2004
    ..Neonatal mice immunised with Pertactin or whole cell Pertussis antigen together with LT were protected against virulent B. pertussis challenge...
  101. pmc Functional cell permeable motifs within medically relevant proteins
    Walter Low
    Biological Sciences, Imperial College London, Imperial College Road, 5th floor SAF Building, London SW7 2AZ, UK
    J Biotechnol 129:555-64. 2007
    ..These results show for the first time the potential of using for therapy cell permeable peptides derived from human proteins involved in disease...