Peter Cherepanov

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. doi request reprint Structural insights into the retroviral DNA integration apparatus
    Peter Cherepanov
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Curr Opin Struct Biol 21:249-56. 2011
  2. pmc LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro
    Peter Cherepanov
    Section of Infectious Diseases, Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, W2 1PG, London, UK
    Nucleic Acids Res 35:113-24. 2007
  3. pmc A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, London, United Kingdom
    PLoS Pathog 5:e1000259. 2009
  4. pmc Retroviral intasome assembly and inhibition of DNA strand transfer
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Nature 464:232-6. 2010
  5. pmc Structural basis for functional tetramerization of lentiviral integrase
    Stephen Hare
    Division of Medicine, St Mary s Campus, Imperial College London, London, United Kingdom
    PLoS Pathog 5:e1000515. 2009
  6. pmc Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding
    Erik Serrao
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA, Division of Infectious Diseases, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK and Clare Hall Laboratories, London Research Institute, Cancer Research UK, Hertfordshire EN6 3LD, UK
    Nucleic Acids Res 42:5164-76. 2014
  7. pmc The mechanism of retroviral integration from X-ray structures of its key intermediates
    Goedele N Maertens
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Nature 468:326-9. 2010
  8. pmc Structural basis for nuclear import of splicing factors by human Transportin 3
    Goedele N Maertens
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, London W2 1PG, United Kingdom
    Proc Natl Acad Sci U S A 111:2728-33. 2014
  9. pmc Functional and structural characterization of the integrase from the prototype foamy virus
    Eugene Valkov
    Division of Medicine, St Mary s Campus, Imperial College London, London, UK
    Nucleic Acids Res 37:243-55. 2009
  10. pmc 3'-processing and strand transfer catalysed by retroviral integrase in crystallo
    Stephen Hare
    Division of Infectious Diseases, Imperial College London, London, UK
    EMBO J 31:3020-8. 2012

Collaborators

Detail Information

Publications28

  1. doi request reprint Structural insights into the retroviral DNA integration apparatus
    Peter Cherepanov
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Curr Opin Struct Biol 21:249-56. 2011
    ....
  2. pmc LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro
    Peter Cherepanov
    Section of Infectious Diseases, Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, W2 1PG, London, UK
    Nucleic Acids Res 35:113-24. 2007
    ..These results confirm that the interaction with LEDGF is conserved within and limited to Lentivirus and strongly argue that the host factor is intimately involved in the catalysis of lentiviral DNA integration...
  3. pmc A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, London, United Kingdom
    PLoS Pathog 5:e1000259. 2009
    ..These findings provide structural basis for the high affinity lentiviral IN-LEDGF interaction and pave the way for development of LEDGF-based targeting technologies for gene therapy...
  4. pmc Retroviral intasome assembly and inhibition of DNA strand transfer
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Nature 464:232-6. 2010
    ..Our findings define the structural basis of retroviral DNA integration, and will allow modelling of the HIV-1 intasome to aid in the development of antiretroviral drugs...
  5. pmc Structural basis for functional tetramerization of lentiviral integrase
    Stephen Hare
    Division of Medicine, St Mary s Campus, Imperial College London, London, United Kingdom
    PLoS Pathog 5:e1000515. 2009
    ..Our structures moreover highlight adaptable changes at the interfaces of individual IN dimers that allow divergent lentiviruses to utilize a highly-conserved, common integration co-factor...
  6. pmc Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding
    Erik Serrao
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA, Division of Infectious Diseases, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK and Clare Hall Laboratories, London Research Institute, Cancer Research UK, Hertfordshire EN6 3LD, UK
    Nucleic Acids Res 42:5164-76. 2014
    ..The S119A preference moreover mimicked wild-type PFV selectivity at these positions. We conclude that HIV-1 IN residue Ser119 and PFV IN residue Ala188 contact analogous tDNA bases to effect virus integration. ..
  7. pmc The mechanism of retroviral integration from X-ray structures of its key intermediates
    Goedele N Maertens
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Nature 468:326-9. 2010
    ..Our results resolve the structural basis for retroviral DNA integration and provide a framework for the design of INs with altered target sequences...
  8. pmc Structural basis for nuclear import of splicing factors by human Transportin 3
    Goedele N Maertens
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, London W2 1PG, United Kingdom
    Proc Natl Acad Sci U S A 111:2728-33. 2014
    ..Our results elucidate the structural bases for nuclear import of splicing factors and the Tnpo3-CPSF6 nexus in HIV-1 biology. ..
  9. pmc Functional and structural characterization of the integrase from the prototype foamy virus
    Eugene Valkov
    Division of Medicine, St Mary s Campus, Imperial College London, London, UK
    Nucleic Acids Res 37:243-55. 2009
    ....
  10. pmc 3'-processing and strand transfer catalysed by retroviral integrase in crystallo
    Stephen Hare
    Division of Infectious Diseases, Imperial College London, London, UK
    EMBO J 31:3020-8. 2012
    ..The ground state structures moreover highlight a striking substrate mimicry utilized by the inhibitors in their binding to the IN active site and suggest ways to improve upon this clinically relevant class of small molecules...
  11. pmc Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity
    Siobhan Hughes
    Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, United Kingdom and
    J Biol Chem 285:541-54. 2010
    ..Our results indicate that the interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK...
  12. pmc Molecular mechanisms of retroviral integrase inhibition and the evolution of viral resistance
    Stephen Hare
    Division of Infectious Diseases, Imperial College London, London W2 1PG, United Kingdom
    Proc Natl Acad Sci U S A 107:20057-62. 2010
    ..Furthermore, our structures predict physical proximity and an interaction between HIV-1 IN mutant residues His148 and Ser/Ala140, rationalizing the coevolution of Q148H and G140S/A mutations in drug-resistant viral strains...
  13. pmc HIV-1 exploits importin 7 to maximize nuclear import of its DNA genome
    Lyubov Zaitseva
    Wohl Virion Centre, Division of Infection and Immunity, University College London, London, UK
    Retrovirology 6:11. 2009
    ..To further characterize the function of imp7 in HIV-1 replication we generated cell lines stably depleted for imp7 and used them in conjunction with infection, cellular fractionation and pull-down assays...
  14. pmc The Interaction Between Lentiviral Integrase and LEDGF: Structural and Functional Insights
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London, W2 1PG, UK E mail
    Viruses 1:780-801. 2009
    ..This review focuses on the structural aspects of the IN-LEDGF interaction and their functional consequences...
  15. pmc Structural and functional analyses of the second-generation integrase strand transfer inhibitor dolutegravir (S/GSK1349572)
    Stephen Hare
    Division of Infectious Diseases, Imperial College London, London, United Kingdom
    Mol Pharmacol 80:565-72. 2011
    ..In addition, our structures suggest that DTG has the ability to subtly readjust its position and conformation in response to structural changes in the active sites of RAL-resistant INs...
  16. doi request reprint Application of general formulas for the correction of a lattice-translocation defect in crystals of a lentiviral integrase in complex with LEDGF
    Stephen Hare
    Division of Medicine, St Mary s Campus, Imperial College London, Norfolk Place, London, UK
    Acta Crystallogr D Biol Crystallogr 65:966-73. 2009
    ....
  17. ncbi request reprint HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells
    Peter Cherepanov
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000, Leuven, Belgium
    J Biol Chem 278:372-81. 2003
    ....
  18. ncbi request reprint LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells
    Goedele Maertens
    Laboratory of Biomolecular Dynamics, Katholieke Universiteit Leuven, Celestijnenlaan 200D, B 3001 Leuven, Belgium
    J Biol Chem 278:33528-39. 2003
    ..We conclude, therefore, that the interaction with LEDGF/p75 accounts for the karyophilic properties and chromosomal targeting of HIV-1 IN...
  19. pmc Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75
    Peter Cherepanov
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:17308-13. 2005
    ....
  20. ncbi request reprint Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75
    Goedele Maertens
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Biol Chem 279:33421-9. 2004
    ..Moreover, a single amino acid change in the NLS was sufficient to exclude the mutant LEDGF/p75 protein from the nucleus and abolish nuclear import of HIV-1 integrase...
  21. ncbi request reprint Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase
    Peter Cherepanov
    Departments of Cancer Immunology and AIDS and Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Biol Chem 279:48883-92. 2004
    ..LEDGF/p75 and HRP2 are predicted to share a similar domain organization and have an evident evolutionary and likely functional relationship...
  22. pmc The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Pathog 4:e1000046. 2008
    ....
  23. pmc LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration
    Ming Chieh Shun
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 21:1767-78. 2007
    ..Akin to yeast retrotransposons, disrupting the lentiviral targeting mechanism significantly perturbs overall integration...
  24. ncbi request reprint Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness
    Shaila Rahman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Virology 357:79-90. 2007
    ..Thus, the relative affinity of the in vitro IN-LEDGF/p75 interaction is not a universal predictor of IN mutant viral fitness...
  25. pmc A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo
    Fanny Turlure
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Nucleic Acids Res 34:1653-65. 2006
    ....
  26. ncbi request reprint Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin
    Goedele N Maertens
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Cell Sci 119:2563-71. 2006
    ..Our results suggest a role for p75 in the Myc regulatory network, and indicate that p75 is a general adaptor protein tethering divergent factors to chromatin through its versatile integrase-binding domain...
  27. ncbi request reprint Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75
    Peter Cherepanov
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 12:526-32. 2005
    ..The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication...
  28. ncbi request reprint In search of authentic inhibitors of HIV-1 integration
    Zeger Debyser
    Rega Institute for Medical Research, KU Leuven, Flanders, Belgium
    Antivir Chem Chemother 13:1-15. 2002
    ..We point to potential pitfalls in defining an inhibitor as an authentic integrase inhibitor, and propose new strategies and technologies for the discovery of authentic HIV integration inhibitors...