Affiliation: Health Protection Agency
- Assessment of a real-time PCR for the detection and characterization of verocytotoxigenic Escherichia coliClaire Jenkins
Microbiology Services Colindale, Health Protection Agency, London, UK
J Med Microbiol 61:1082-5. 2012..coli O157 using the Health Protection Agency national standard culture method. This study showed that the EU-RL VTEC assay was reliable and robust, and an effective rapid screening method for the detection of VTEC from stool specimens...
- Detection and identification of bacteria in clinical samples by 16S rRNA gene sequencing: comparison of two different approaches in clinical practiceClaire Jenkins
Department of Medical Microbiology, Royal Free Hospital NHS Trust, Pond Street, London NW3 2PF, UK
J Med Microbiol 61:483-8. 2012..The 1343 bp PCR may be used in combination with the 762/598 bp PCR when identification of the bacterial rRNA gene to species level is required...
- Subtyping intimin genes from enteropathogenic Escherichia coli associated with outbreaks and sporadic cases in the United Kingdom and EireC Jenkins
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Avenue, NW9 5HT, London, UK
Mol Cell Probes 17:149-56. 2003..The beta-intimin gene was the most frequently detected sub-type in both the outbreak and sporadic strains...
- Subtyping of virulence genes in verocytotoxin-producing Escherichia coli (VTEC) other than serogroup O157 associated with disease in the United KingdomC Jenkins
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK
J Med Microbiol 52:941-7. 2003..None of the strains from the healthy control group carried the eae gene...
- An eight-month study of a population of verocytotoxigenic Escherichia coli (VTEC) in a Scottish cattle herdC Jenkins
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK
J Appl Microbiol 93:944-53. 2002..Characteristics of these strains were examined to allow comparisons with strains of VTEC associated with human infection...
- Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniquesC Jenkins
Laboratory of Enteric Pathogens, Health Protection Agency, 61 Colindale Avenue, London, NW9 5HT, UK
Lett Appl Microbiol 37:207-12. 2003....
- Serotypes, intimin subtypes, and antimicrobial resistance patterns of atypical enteropathogenic Escherichia coli isolated in England from 1993 to 1996C Jenkins
Department of Medical Microbiology, Royal Free Hospital, London, NW3 2QG, UK
Eur J Clin Microbiol Infect Dis 25:19-24. 2006..002). The compilation of data on atypical EPEC strains presented here indicates the need for further study of their virulence and epidemiology in order to assess their significance as human pathogens...
- Association of putative pathogenicity genes with adherence characteristics and fimbrial genotypes in typical enteroaggregative Escherichia coli from patients with and without diarrhoea in the United KingdomC Jenkins
Department of Microbiology, Royal Free Hospital, London, UK
Eur J Clin Microbiol Infect Dis 26:901-6. 2007..We conclude that in addition to presence and absence of certain genes, studies of EAggEC pathogenicity should investigate the combinations and associations of putative virulence factors...
- Verocytotoxin-producing Escherichia coli (VTEC) other than serogroup O157 from Scottish cattleC Jenkins
Laboratory of Enteric Pathogens, Central Public Health Authority, London
Vet Rec 151:58-60. 2002
- Escherichia coli serogroup O26--a new look at an old adversaryC Jenkins
Department of Medical Microbiology, Royal Free Hospital, NW3 2QG, London, UK
J Appl Microbiol 104:14-25. 2008..The increase in awareness of VTEC O26 in the clinical and veterinary setting has coincided with the development of novel techniques that have improved our ability to detect and characterize this pathogen...
- Haemolysin production by strains of Verocytotoxin-producing Escherichia coliH Chart
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK
Microbiology 144:103-7. 1998..It was concluded that haemolysins produced by VTEC appear to be quite distinct from E. coli alpha-haemolysin and resemble a form of beta-haemolysin...
- The expression of an R3 lipopolysaccharide-core by pathotypes of Escherichia coliH Chart
Laboratory of Enteric Pathogens, Division of Gastrointestinal Infections, Central Public Health Laboratory, London, UK
J Appl Microbiol 96:982-6. 2004..coli (VTEC), enteroaggregative E. coli (EAggEC) and enteropathogenic E. coli (EPEC)...
- Disease severity of Shiga toxin-producing E. coli O157 and factors influencing the development of typical haemolytic uraemic syndrome: a retrospective cohort study, 2009-2012N Launders
Gastrointestinal, Emerging and Zoonotic Infections Department, Public Health England, Centre for Infectious Disease Surveillance and Control, London, UK
BMJ Open 6:e009933. 2016..Assess the disease severity of Shiga toxin-producing Escherichia coli (STEC) O157 infection and factors influencing the development of typical haemolytic uraemic syndrome (tHUS)...
- Retrospective analysis of whole genome sequencing compared to prospective typing data in further informing the epidemiological investigation of an outbreak of Shigella sonnei in the UKJ McDonnell
North East and North Central London Health Protection Unit, Health Protection Agency, London, UK
Epidemiol Infect 141:2568-75. 2013....
- Blinded application of microscopy, bacteriological culture, immunoassays and PCR to detect gastrointestinal pathogens from faecal samples of patients with community-acquired diarrhoeaC F L Amar
Health Protection Agency, Food Safety Microbiology Laboratory, 61 Colindale Avenue, London, NW9 5HT, UK
Eur J Clin Microbiol Infect Dis 23:529-34. 2004..coli since these were only investigated by PCR. The results of this small study clearly demonstrate the advantages of PCR-based methods compared to conventional techniques for the detection of gastrointestinal pathogens...
- The epidemiology, microbiology and clinical impact of Shiga toxin-producing Escherichia coli in England, 2009-2012L Byrne
Public Health England Department of Gastrointestinal, Emerging and Zoonotic Infections, Centre for Infectious Disease Surveillance and Control, London, UK
Epidemiol Infect 143:3475-87. 2015..Implementation of PCR-based diagnostic methods designed to detect all STEC, to address this diagnostic deficit, is therefore important. ..
- When is an outbreak not an outbreak? Fit, divergent strains of Mycobacterium tuberculosis display independent evolution of drug resistance in a large London outbreakR J Shorten
Department of Medical Microbiology, Royal Free Hospital, London, UK
J Antimicrob Chemother 68:543-9. 2013..To study the evolutionary relationship of Mycobacterium tuberculosis isolates from 13 patients in a large outbreak of isoniazid-resistant tuberculosis in London...
- Investigation of a national outbreak of STEC Escherichia coli O157 using online consumer panel control methods: Great Britain, October 2014C Sinclair
UK Field Epidemiology Training Programme, Public Health England, London, UK
Epidemiol Infect . 2016..Collaboration with industrial bodies is needed to ensure timely traceback exercises to identify contamination events and initiate appropriate and focused microbiological testing and implement control measures...
- The utility and public health implications of PCR and whole genome sequencing for the detection and investigation of an outbreak of Shiga toxin-producing Escherichia coli serogroup O26:H11T J Dallman
Gastrointestinal and Emerging Zoonotic Infections Department, Public Health England, London, UK
Epidemiol Infect 143:1672-80. 2015..During this study, we describe the utility of PCR and the genome sequencing approach in facilitating surveillance and enhancing the response to outbreaks of non-O157 STEC. ..
- Microbiological aspects of public health planning and preparedness for the 2012 Olympic GamesJ Moran-Gilad
Health Protection Agency, Microbiology Services, UK
Epidemiol Infect 140:2142-51. 2012..These will deliver durable legacies for clinical and public health microbiology, outbreak investigation and control in the coming years...
- Modelling the epidemiology of Verocytotoxin-producing Escherichia coli serogroups in young calvesW C Liu
Centre for Tropical Veterinary Medicine, University of Edinburgh, Easter Bush, Roslin, Midlothian, Scotland, EH25 9RG, UK
Epidemiol Infect 133:449-58. 2005..We suggest that future field studies may wish to adopt a tighter sampling frame in order to detect all circulating VTEC serogroups in similar animal populations...
- Shedding patterns of verocytotoxin-producing Escherichia coli strains in a cohort of calves and their dams on a Scottish beef farmD J Shaw
Veterinary Epidemiology Group, Centre for Tropical and Veterinary Medicine, College of Medicine and Veterinary Medicine, University of Edinburgh, Roslin, Midlothian, United Kingdom
Appl Environ Microbiol 70:7456-65. 2004..No association between calf diarrhea and any of the VTEC serogroups was identified...
- Temporal shedding patterns and virulence factors of Escherichia coli serogroups O26, O103, O111, O145, and O157 in a cohort of beef calves and their damsM C Pearce
Centre for Tropical Veterinary Medicine, University of Edinburgh, Roslin, Midlothian EH25 9RG, United Kingdom
Appl Environ Microbiol 70:1708-16. 2004..For E. coli O26 and O103, there was no association between shedding and scouring, and there was no significant change in shedding following housing...
- A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratoryK J Williams
Department of Microbiology, Royal Free Hospital, London NW3 2QG, UK
J Med Microbiol 56:598-602. 2007..It provides a useful tool for the identification of MOTT when clinically indicated...
- Phylogenetic analysis of evolutionary relationships of the planctomycete division of the domain bacteria based on amino acid sequences of elongation factor TuC Jenkins
Department of Microbiology, University of Washington, Seattle, WA 98195-7242, USA
J Mol Evol 52:405-18. 2001..It is hypothesized that long-branch attraction effects due to unequal evolutionary rates and mutational saturation effects may account for some of the contradictions...
- Molecular structure of the mouse CCK-A receptor geneK A Lacourse
Department of Physiology, The University of Michigan, Ann Arbor 48109 0622, USA
Biochem Biophys Res Commun 236:630-5. 1997..Cckar is widely expressed in the gastrointestinal system (pancreas, gallbladder, intestine, colon and stomach), as well as in brain and kidney...