Michel Doumith

Summary

Affiliation: Health Protection Agency
Country: UK

Publications

  1. doi request reprint Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK
    Michel Doumith
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency, Centre for Infections, 61 Colindale Avenue, London NW95EQ, UK
    J Antimicrob Chemother 63:659-67. 2009
  2. doi request reprint Characterization of plasmids encoding extended-spectrum β-lactamases and their addiction systems circulating among Escherichia coli clinical isolates in the UK
    Michel Doumith
    Antibiotic Resistance Monitoring and Reference Laboratory, HPA Colindale, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 67:878-85. 2012
  3. pmc Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups
    M Doumith
    Microbiology Services Colindale, Health Protection Agency, London, United Kingdom
    J Clin Microbiol 50:3108-10. 2012
  4. doi request reprint Emergence of AcrAB-mediated tigecycline resistance in a clinical isolate of Enterobacter cloacae during ciprofloxacin treatment
    Michael Hornsey
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, London NW9 5EQ, UK
    Int J Antimicrob Agents 35:478-81. 2010
  5. doi request reprint AdeABC-mediated efflux and tigecycline MICs for epidemic clones of Acinetobacter baumannii
    Michael Hornsey
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 65:1589-93. 2010
  6. doi request reprint False extended-spectrum {beta}-lactamase phenotype in clinical isolates of Escherichia coli associated with increased expression of OXA-1 or TEM-1 penicillinases and loss of porins
    Alejandro Beceiro
    Health Protection Agency, Microbiology Services Colindale, London, UK
    J Antimicrob Chemother 66:2006-10. 2011
  7. doi request reprint Molecular epidemiology of fluoroquinolone-resistant ST131 Escherichia coli producing CTX-M extended-spectrum beta-lactamases in nursing homes in Belfast, UK
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 66:297-303. 2011
  8. doi request reprint Isolation of fluoroquinolone-resistant O25b:H4-ST131 Escherichia coli with CTX-M-14 extended-spectrum β-lactamase from UK river water
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Microbiology Services Colindale, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 66:512-6. 2011
  9. doi request reprint ISEcp1-mediated transposition of linked blaCTX-M-3 and blaTEM-1b from the IncI1 plasmid pEK204 found in clinical isolates of Escherichia coli from Belfast, UK
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Microbiology Services Colindale, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 66:2263-5. 2011
  10. doi request reprint What remains against carbapenem-resistant Enterobacteriaceae? Evaluation of chloramphenicol, ciprofloxacin, colistin, fosfomycin, minocycline, nitrofurantoin, temocillin and tigecycline
    David M Livermore
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Microbiology Services Colindale, 61 Colindale Avenue, London NW9 5EQ, UK
    Int J Antimicrob Agents 37:415-9. 2011

Collaborators

Detail Information

Publications31

  1. doi request reprint Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK
    Michel Doumith
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency, Centre for Infections, 61 Colindale Avenue, London NW95EQ, UK
    J Antimicrob Chemother 63:659-67. 2009
    ..n = 28) and Enterobacter spp. (n = 27) referred from multiple hospitals to the UK national reference laboratory...
  2. doi request reprint Characterization of plasmids encoding extended-spectrum β-lactamases and their addiction systems circulating among Escherichia coli clinical isolates in the UK
    Michel Doumith
    Antibiotic Resistance Monitoring and Reference Laboratory, HPA Colindale, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 67:878-85. 2012
    ..To characterize plasmids encoding extended-spectrum β-lactamases (ESBLs) from a recent UK collection of clinical Escherichia coli isolates...
  3. pmc Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups
    M Doumith
    Microbiology Services Colindale, Health Protection Agency, London, United Kingdom
    J Clin Microbiol 50:3108-10. 2012
    ..It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data...
  4. doi request reprint Emergence of AcrAB-mediated tigecycline resistance in a clinical isolate of Enterobacter cloacae during ciprofloxacin treatment
    Michael Hornsey
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, London NW9 5EQ, UK
    Int J Antimicrob Agents 35:478-81. 2010
    ..Treatment with ciprofloxacin, a substrate of AcrAB in E. cloacae, possibly selected for cross-resistance to tigecycline as a result of RamA-mediated AcrAB upregulation...
  5. doi request reprint AdeABC-mediated efflux and tigecycline MICs for epidemic clones of Acinetobacter baumannii
    Michael Hornsey
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 65:1589-93. 2010
    ..baumannii clones. The role of AdeABC in the emergence of tigecycline resistance during therapy was also investigated for two representatives of the prevalent UK lineage, OXA-23 clone 1...
  6. doi request reprint False extended-spectrum {beta}-lactamase phenotype in clinical isolates of Escherichia coli associated with increased expression of OXA-1 or TEM-1 penicillinases and loss of porins
    Alejandro Beceiro
    Health Protection Agency, Microbiology Services Colindale, London, UK
    J Antimicrob Chemother 66:2006-10. 2011
    ..EC19, from the same patient as EC21, was susceptible to all four cephalosporins. We sought to characterize the molecular basis of resistance in isolates EC18 and EC21...
  7. doi request reprint Molecular epidemiology of fluoroquinolone-resistant ST131 Escherichia coli producing CTX-M extended-spectrum beta-lactamases in nursing homes in Belfast, UK
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 66:297-303. 2011
    ..Of these, 58 isolates belonged to UK strain A, a variant of the international ST131 clone. Here we investigated the remaining 60 ESBL producers...
  8. doi request reprint Isolation of fluoroquinolone-resistant O25b:H4-ST131 Escherichia coli with CTX-M-14 extended-spectrum β-lactamase from UK river water
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Microbiology Services Colindale, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 66:512-6. 2011
    ....
  9. doi request reprint ISEcp1-mediated transposition of linked blaCTX-M-3 and blaTEM-1b from the IncI1 plasmid pEK204 found in clinical isolates of Escherichia coli from Belfast, UK
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Microbiology Services Colindale, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 66:2263-5. 2011
    ..We investigated the mechanisms for their joint dissemination among diverse plasmids...
  10. doi request reprint What remains against carbapenem-resistant Enterobacteriaceae? Evaluation of chloramphenicol, ciprofloxacin, colistin, fosfomycin, minocycline, nitrofurantoin, temocillin and tigecycline
    David M Livermore
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Microbiology Services Colindale, 61 Colindale Avenue, London NW9 5EQ, UK
    Int J Antimicrob Agents 37:415-9. 2011
    ..Studies with transconjugants and transformants confirmed the small effect of KPC enzymes against temocillin, whereas OXA-48 and NDM-1 conferred clear resistance...
  11. pmc Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients
    Maria Tomas
    Centre for Infections, Health Protection Agency, London, United Kingdom
    Antimicrob Agents Chemother 54:2219-24. 2010
    ..Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA...
  12. doi request reprint Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London NW9 5EQ, UK
    Int J Antimicrob Agents 36:355-8. 2010
    ..coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like...
  13. pmc Phosphoethanolamine modification of lipid A in colistin-resistant variants of Acinetobacter baumannii mediated by the pmrAB two-component regulatory system
    Alejandro Beceiro
    Health Protection Agency, Microbiology Services Colindale, London, United Kingdom
    Antimicrob Agents Chemother 55:3370-9. 2011
    ..baumannii requires distinct genetic events, including (i) at least one point mutation in pmrB, (ii) upregulation of pmrAB, and (iii) expression of pmrC, which lead to addition of phosphoethanolamine to lipid A...
  14. doi request reprint Variation in the genetic environments of bla(CTX-M-15) in Escherichia coli from the faeces of travellers returning to the United Kingdom
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Microbiology Services Colindale, London, UK
    J Antimicrob Chemother 66:1005-12. 2011
    ....
  15. doi request reprint Cephalosporin resistance mechanisms in Escherichia coli isolated from raw chicken imported into the UK
    Hiran Dhanji
    Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London, UK
    J Antimicrob Chemother 65:2534-7. 2010
    ..coli belonging to the international uropathogenic ST131 clone...
  16. doi request reprint Whole-genome comparison of two Acinetobacter baumannii isolates from a single patient, where resistance developed during tigecycline therapy
    Michael Hornsey
    Antimicrobial Research Group, Centre for Immunology and Infectious Disease, Blizard Institute, Barts and The London, Queen Mary s School of Medicine and Dentistry, 4 Newark Street, London, E1 2AT, UK
    J Antimicrob Chemother 66:1499-503. 2011
    ..The first, AB210, was recovered before tigecycline therapy and was susceptible to this agent; the second, AB211, was recovered after therapy and was resistant...
  17. pmc KPC enzymes in the UK: an analysis of the first 160 cases outside the North-West region
    Jacqueline Findlay
    Antimicrobial Resistance and Healthcare Associated Infections AMRHAI Reference Unit, Public Health England, London NW9 5EQ, UK
    J Antimicrob Chemother 71:1199-206. 2016
    ....
  18. doi request reprint Tigecycline resistance in Serratia marcescens associated with up-regulation of the SdeXY-HasF efflux system also active against ciprofloxacin and cefpirome
    Michael Hornsey
    Antibiotic Resistance Monitoring and Reference Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother 65:479-82. 2010
    ....
  19. doi request reprint The differential importance of mutations within AmpD in cephalosporin resistance of Enterobacter aerogenes and Enterobacter cloacae
    Baharak Babouee Flury
    Antimicrobial Resistance and Healthcare Associated Infections AMRHAI Reference Unit, National Infection Service, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK Electronic address
    Int J Antimicrob Agents 48:555-558. 2016
    ..aerogenes underlines the differences between these contrasting members of the Enterobacter genus. Porin loss resulted in high-level carbapenem resistance with gradual loss of Omp36, which led to high-level meropenem resistance...
  20. doi request reprint Detection of the plasmid-mediated mcr-1 gene conferring colistin resistance in human and food isolates of Salmonella enterica and Escherichia coli in England and Wales
    Michel Doumith
    National Infection Service, Public Health England, London NW9 5EQ, UK
    J Antimicrob Chemother 71:2300-5. 2016
    ..from animals and humans in China, we sought to determine its presence in Enterobacteriaceae isolated in the UK...
  21. pmc Association of Novel Nonsynonymous Single Nucleotide Polymorphisms in ampD with Cephalosporin Resistance and Phylogenetic Variations in ampC, ampR, ompF, and ompC in Enterobacter cloacae Isolates That Are Highly Resistant to Carbapenems
    Baharak Babouee Flury
    Antimicrobial Resistance and Healthcare Associated Infections AMRHAI Reference Unit, Public Health England, London, United Kingdom
    Antimicrob Agents Chemother 60:2383-90. 2016
    ..The additional loss of porins resulted in high-level carbapenem resistance, underlining the clinical importance of chromosomal mutations among carbapenem-resistantE. cloacae. ..
  22. pmc Activities of NXL104 combinations with ceftazidime and aztreonam against carbapenemase-Producing Enterobacteriaceae
    David M Livermore
    Antibiotic Resistance Monitoring and Reference Laboratory, HPA Centre for Infections, London, United Kingdom
    Antimicrob Agents Chemother 55:390-4. 2011
    ..with the KPC enzyme, but metallo-β-lactamase producers were resistant. Aztreonam-NXL104 was active against all carbapenemase producers at 4 and 4 μg/ml, including those with metallo-β-lactamases...
  23. doi request reprint Clonal expansion of Escherichia coli ST38 carrying a chromosomally integrated OXA-48 carbapenemase gene
    Jane F Turton
    Antimicrobial Resistance and Healthcare Associated Infections AMRHAI Reference Unit, Public Health England, London NW9 5EQ, UK
    J Med Microbiol 65:538-46. 2016
    ..coli sequence on one side of the insertion. This sub-cluster of ST38 was found from 25 different hospital laboratories, suggesting widespread distribution of a successful type. ..
  24. pmc Rise of multidrug-resistant non-vaccine serotype 15A Streptococcus pneumoniae in the United Kingdom, 2001 to 2014
    Carmen Sheppard
    Respiratory and Vaccine Preventable Bacteria Reference Unit, Public Health England, London, United Kingdom
    Euro Surveill 21:. 2016
    ..Almost all multidrug-resistant 15A isolates were sequence type (ST) 63 variants, whereas susceptible 15A isolates were clonally diverse. The rise of serotype 15A suggests that pneumococcal conjugate vaccines will need ongoing adaptation...
  25. ncbi request reprint WGS for surveillance of antimicrobial resistance: a pilot study to detect the prevalence and mechanism of resistance to azithromycin in a UK population of non-typhoidal Salmonella
    Satheesh Nair
    Gastrointestinal Bacteria Reference Unit, Public Health England, London NW9 5EQ, UK
    J Antimicrob Chemother 71:3400-3408. 2016
    ..WGS and phenotypic methods were used to determine the prevalence of azithromycin resistance in Salmonella enterica isolates from the UK and to identify the underlying mechanisms of resistance...
  26. ncbi request reprint Antimicrobial resistance in Shiga toxin-producing Escherichia coli serogroups O157 and O26 isolated from human cases of diarrhoeal disease in England, 2015
    Martin Day
    Bacteriology Reference Department, Reference Microbiology Services, National Infection Service, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK
    J Antimicrob Chemother . 2016
    ..In this study, WGS data were used to predict antimicrobial resistance (AMR) in STEC from symptomatic human cases to assess the extent of transmission of antibiotic-resistant E. coli from animals to humans...
  27. pmc High-Resolution Analysis by Whole-Genome Sequencing of an International Lineage (Sequence Type 111) of Pseudomonas aeruginosa Associated with Metallo-Carbapenemases in the United Kingdom
    Jane F Turton
    Antimicrobial Resistance and Healthcare Associated Infections AMRHAI Reference Unit, Public Health England, Colindale, London, United Kingdom
    J Clin Microbiol 53:2622-31. 2015
    ..WGS of widely found international lineages such as ST-111 provides the necessary resolution to inform epidemiological investigations and intervention policies. ..
  28. doi request reprint NDM carbapenemases in the United Kingdom: an analysis of the first 250 cases
    Anu Jain
    Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, London NW9 5EQ, UK
    J Antimicrob Chemother 69:1777-84. 2014
    ..We analysed patient data for NDM-positive isolates confirmed by the national reference laboratory from UK laboratories from February 2008 to July 2013...
  29. doi request reprint In vitro activity of rifaximin against clinical isolates of Escherichia coli and other enteropathogenic bacteria isolated from travellers returning to the UK
    Katie L Hopkins
    Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK Electronic address
    Int J Antimicrob Agents 43:431-7. 2014
    ..coli in the gut flora, but much stronger associations were seen between ESBL and/or carbapenemase production and resistance to alternative treatments for travellers' diarrhoea, notably ciprofloxacin and azithromycin...
  30. doi request reprint Use of nrdA gene sequence clustering to estimate the prevalence of different Achromobacter species among Cystic Fibrosis patients in the UK
    Amy Coward
    Antimicrobial Resistance and Healthcare Associated Infections AMRHAI Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, United Kingdom
    J Cyst Fibros 15:479-85. 2016
    ..We aimed to estimate the prevalence of different Achromobacter species among UK Cystic Fibrosis (CF) patients...
  31. pmc An investigation of the diversity of strains of enteroaggregative Escherichia coli isolated from cases associated with a large multi-pathogen foodborne outbreak in the UK
    Timothy J Dallman
    Gastrointestinial Bacteria Reference Unit, Public Health England, London, United Kingdom
    PLoS ONE 9:e98103. 2014
    ..Although the EAEC in this study did not carry the stx genes, this outbreak provides further evidence of the pathogenic potential of the EAEC O104:H4 serotype. ..