N Audsley

Summary

Affiliation: Central Science Laboratory
Country: UK

Publications

  1. ncbi request reprint The significance of Manduca sexta allatostatin in the tomato moth Lacanobia oleracea
    N Audsley
    Central Science Laboratory, Sand Hutton, York, United Kingdom
    Ann N Y Acad Sci 897:330-41. 1999
  2. ncbi request reprint Neuropeptides associated with the frontal ganglion of larval Lepidoptera
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 26:11-21. 2005
  3. ncbi request reprint Endopeptidase activity of larval Lacanobia oleracea corpus allatum: metabolism of Manduca sexta allatostatin and allatotropin
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York, United Kingdom
    Arch Insect Biochem Physiol 57:178-89. 2004
  4. ncbi request reprint Metabolism of cydiastatin 4 and analogues by enzymes associated with the midgut and haemolymph of Manduca sexta larvae
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Gen Comp Endocrinol 153:80-7. 2007
  5. ncbi request reprint Identification of neuropeptides from brains of larval Manduca sexta and Lacanobia oleracea using MALDI-TOF mass spectrometry and post-source decay
    Neil Audsley
    Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK
    Peptides 24:1465-74. 2003
  6. ncbi request reprint A comparison of the neuropeptides from the retrocerebral complex of adult male and female Manduca sexta using MALDI-TOF mass spectrometry
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Regul Pept 116:127-37. 2003
  7. doi request reprint Transepithelial flux of an allatostatin and analogs across the anterior midgut of Manduca sexta larvae in vitro
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 29:286-94. 2008
  8. ncbi request reprint Degradation of Manduca sexta allatostatin and allatotropin by proteases associated with the foregut of Lacanobia oleracea larvae
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 23:2015-23. 2002
  9. doi request reprint Allatoregulatory peptides in Lepidoptera, structures, distribution and functions
    N Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    J Insect Physiol 54:969-80. 2008
  10. doi request reprint Neuropeptides associated with the regulation of feeding in insects
    N Audsley
    Environmental Biology Group, Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Gen Comp Endocrinol 162:93-104. 2009

Collaborators

Detail Information

Publications34

  1. ncbi request reprint The significance of Manduca sexta allatostatin in the tomato moth Lacanobia oleracea
    N Audsley
    Central Science Laboratory, Sand Hutton, York, United Kingdom
    Ann N Y Acad Sci 897:330-41. 1999
    ..In adult male brains, even though relatively high levels of immunoreactivity co-elute with synthetic Mas-As, the majority of the Mas-AS-like immunoreactivity is more hydrophobic...
  2. ncbi request reprint Neuropeptides associated with the frontal ganglion of larval Lepidoptera
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 26:11-21. 2005
    ..In HPLC fractions of M. sexta frontal ganglia, F/YXFGL-NH2 allatostatin-like immunoreactivity was widespread suggesting that more allatostatins were present than were identified...
  3. ncbi request reprint Endopeptidase activity of larval Lacanobia oleracea corpus allatum: metabolism of Manduca sexta allatostatin and allatotropin
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York, United Kingdom
    Arch Insect Biochem Physiol 57:178-89. 2004
    ..A trypsin-like peptidase appears to be the major enzyme present in the CA of larval L. oleracea that acts to metabolise Manse-AS and Manse-AT. In addition, an unidentified enzyme that cleaves between M and T residues degraded Manse-AT...
  4. ncbi request reprint Metabolism of cydiastatin 4 and analogues by enzymes associated with the midgut and haemolymph of Manduca sexta larvae
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Gen Comp Endocrinol 153:80-7. 2007
    ..No metabolism of cydiastatin 4beta occurred when incubated with larval M. sexta haemolymph over a 90min period...
  5. ncbi request reprint Identification of neuropeptides from brains of larval Manduca sexta and Lacanobia oleracea using MALDI-TOF mass spectrometry and post-source decay
    Neil Audsley
    Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK
    Peptides 24:1465-74. 2003
    ..oleracea brain extracts. From this insect, cydiastatins 3 and 4, helicostatin 5 and FLRF-amide I were identified by PSD. These peptides had not previously been structurally characterised from L. oleracea...
  6. ncbi request reprint A comparison of the neuropeptides from the retrocerebral complex of adult male and female Manduca sexta using MALDI-TOF mass spectrometry
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Regul Pept 116:127-37. 2003
    ..Fragmentation by collision-induced dissociation MS/MS identified an extended AKH peptide. The apparent differences in the peptides present in male and female retrocerebral complexes are most likely quantitative rather than sex specific...
  7. doi request reprint Transepithelial flux of an allatostatin and analogs across the anterior midgut of Manduca sexta larvae in vitro
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 29:286-94. 2008
    ....
  8. ncbi request reprint Degradation of Manduca sexta allatostatin and allatotropin by proteases associated with the foregut of Lacanobia oleracea larvae
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 23:2015-23. 2002
    ..The second product was identified as Manse-AT-(1-8). Hydrolysis of Manse-AT could only be partially inhibited by high doses of aprotinin (30%)...
  9. doi request reprint Allatoregulatory peptides in Lepidoptera, structures, distribution and functions
    N Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    J Insect Physiol 54:969-80. 2008
    ..There is also evidence that they are expressed in the midgut and reproductive tissues...
  10. doi request reprint Neuropeptides associated with the regulation of feeding in insects
    N Audsley
    Environmental Biology Group, Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Gen Comp Endocrinol 162:93-104. 2009
    ..These neuropeptides may also act at sites other than visceral muscles, such as centrally through the brain or on gut stretch receptors...
  11. ncbi request reprint Metabolism of Manduca sexta allatostatin by hemolymph of larvae of the tomato moth, Lacanobia oleracea
    Neil Audsley
    Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK
    Peptides 23:717-23. 2002
    ..These metabolites were synthesized and assayed for biological activity on juvenile hormone (JH) biosynthesis in vitro. All three of the above deletion peptides showed allatostatin activity, but were not as potent as Manse-AS (1-15)...
  12. ncbi request reprint Juvenile hormone biosynthesis by corpora allata of larval tomato moth, Lacanobia oleracea, and regulation by Manduca sexta allatostatin and allatotropin
    N Audsley
    Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK
    Insect Biochem Mol Biol 30:681-9. 2000
    ..oleracea brain homogenates. Co-elution of this immunoreactive peak with synthetic Mas-AT suggests that this neuropeptide is also present in L. oleracea...
  13. ncbi request reprint In vitro transport of an allatostatin across the foregut of Manduca sexta larvae and metabolism by the gut and hemolymph
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 28:136-45. 2007
    ..In contrast, no trans-epithelial transport of peptide was apparent across everted foregut sac preparations...
  14. doi request reprint Degradation of leucomyosuppressin by enzymes in the hemolymph and midgut of Lacanobia oleracea and Spodoptera littoralis (Lepidoptera: Noctuidae) larvae
    H J Matthews
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 30:565-70. 2009
    ..oleracea or S. littoralis, t(1/2)=40.7 and 69.8 min, respectively. The most abundant degradation products probably resulted from carboxypeptidase activity, but some aminopeptidase activity was also detected...
  15. ncbi request reprint Interactions between allatostatins and allatotropin on spontaneous contractions of the foregut of larval Lacanobia oleracea
    H J Matthews
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    J Insect Physiol 53:75-83. 2007
    ..These results question the need for two structurally different allatostatins that have the same physiological effect on foregut peristalsis in L. oleracea larvae...
  16. ncbi request reprint Alanine substitution and deletion analogues of Manduca sexta allatostatin: structure-activity relationship on the spontaneous contractions of the foregut of larval Lacanobia oleracea
    H J Matthews
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    J Insect Physiol 52:128-35. 2006
    ..The results show that amino acid residues both inside and outside the disulphide ring are important for biological activity...
  17. doi request reprint In vitro and in vivo effects of myo-active peptides on larvae of the tomato moth Lacanobia oleracea and the cotton leaf worm Spodoptera littoralis (Lepidoptera; Noctuidae)
    H J Matthews
    Central Science Laboratory, Sand Hutton, York, United Kingdom
    Arch Insect Biochem Physiol 69:60-9. 2008
    ....
  18. ncbi request reprint The role of allatostatic and allatotropic neuropeptides in the regulation of juvenile hormone biosynthesis in Lacanobia oleracea (Lepidoptera: Noctuidae)
    J P Edwards
    Central Science Laboratory, Ministry of Agriculture, Fisheries and Food, Sand Hutton, York YO41 1LZ, United Kingdom
    Peptides 22:255-61. 2001
    ..sexta allatotropic and allatostatic neuropeptides in L. oleracea...
  19. ncbi request reprint Analysis of peptides in the brain and corpora cardiaca-corpora allata of the honey bee, Apis mellifera using MALDI-TOF mass spectrometry
    Neil Audsley
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Peptides 27:512-20. 2006
    ..No ion signal was detected that would correspond to the hypertrehalosaemic peptide (=Manse-AKH), which has been isolated from the Italian race of the honey bee (A. mellifera ligustica), but not from A. mellifera carnica...
  20. doi request reprint Neuropeptide regulators of juvenile hormone synthesis: structures, functions, distribution, and unanswered questions
    R J Weaver
    Environmental Biology Group, Central Science Laboratory, Sand Hutton, York, United Kingdom
    Ann N Y Acad Sci 1163:316-29. 2009
    ..Other neuropeptides are implicated in the regulation of JH production, and there is new evidence concerning the mode of action of allatotropins...
  21. doi request reprint Neuropeptides of the beetle, Tenebrio molitor identified using MALDI-TOF mass spectrometry and deduced sequences from the Tribolium castaneum genome
    Robert J Weaver
    Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
    Peptides 29:168-78. 2008
    ..No evidence was found for the presence of 'A' allatostatins (Y/FxFGLamides) or corazonin, either in T. molitor, or in the genome of T. castaneum...
  22. ncbi request reprint The ectoparasitic wasp Eulophus pennicornis (Hymenoptera: Eulophidae) uses instar-specific endocrine disruption strategies to suppress the development of its host Lacanobia oleracea (Lepidoptera: Noctuidae)
    John P Edwards
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    J Insect Physiol 52:1153-62. 2006
    ..We conclude that E. pennicornis employs host endocrine-disruption strategies that differ according to whether the host is parasitized as a penultimate or final-stadium larva...
  23. ncbi request reprint Towards a comprehensive view of the primary structure of venom proteins from the parasitoid wasp Pimpla hypochondriaca
    Neil M Parkinson
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Insect Biochem Mol Biol 34:565-71. 2004
    ..The random sequencing approach has provided a rapid means of determining the primary structure of the majority of Pimpla hypochondriaca venom proteins...
  24. ncbi request reprint Fusion proteins containing neuropeptides as novel insect contol agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion
    Elaine Fitches
    Central Science Laboratory, Department for Environment, Food and Rural Affairs, Sand Hutton, York YO41 1LZ, UK
    Insect Biochem Mol Biol 32:1653-61. 2002
    ....
  25. doi request reprint Effects of Manduca sexta allatostatin and an analog on the pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) and degradation by enzymes from the aphid gut
    Rachel E Down
    The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK
    Peptides 31:489-97. 2010
    ..The increased stability of the Manse-AS analog may explain its enhanced feeding suppression effects when continually fed to aphids, and demonstrates the potential use of Manse-AS in a strategy to control aphid pests...
  26. doi request reprint MALDI-TOF mass spectrometry approaches to the characterisation of insect neuropeptides
    Robert J Weaver
    The Food and Environment Research Agency, Sand Hutton, York, UK
    Methods Mol Biol 615:101-15. 2010
    ..Some of the procedures that are now used to identify these peptides are described here. Once the neuropeptides have been identified, it then becomes possible to use this knowledge to define physiological functionality...
  27. ncbi request reprint Purification of pimplin, a paralytic heterodimeric polypeptide from venom of the parasitoid wasp Pimpla hypochondriaca, and cloning of the cDNA encoding one of the subunits
    N Parkinson
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Insect Biochem Mol Biol 32:1769-73. 2002
    ..Beginning at the fourth residue of the mature pim1 venom polypeptide is a stretch of 46 residues consisting of alternating prolines, the significance of which is discussed in terms of possible host processing...
  28. doi request reprint The insecticidal activity of recombinant garlic lectins towards aphids
    Elaine Fitches
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Insect Biochem Mol Biol 38:905-15. 2008
    ..A pull-down assay coupled with peptide mass fingerprinting identified two abundant membrane-associated aphid gut proteins, alanyl aminopeptidase N and sucrase, as "receptors" for lectin binding...
  29. ncbi request reprint Screening method for the addition of bovine blood-based binding agents to food using liquid chromatography triple quadrupole mass spectrometry
    Helen H Grundy
    DEFRA Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Rapid Commun Mass Spectrom 21:2919-25. 2007
    ..Fibrinopeptide A was found to be an effective marker in fresh, processed and cooked food matrices spiked with 5% (v/w) bovine binding agent...
  30. doi request reprint Predicted versus expressed adipokinetic hormones, and other small peptides from the corpus cardiacum-corpus allatum: a case study with beetles and moths
    Gerd Gäde
    Zoology Department, University of Cape Town, Private Bag, ZA 7701 Rondebosch, South Africa
    Peptides 29:1124-39. 2008
    ..In L. oleracea, however, the latter peptide has a novel sequence which is reported for the first time, and the peptide is code-named Lacol-PK...
  31. ncbi request reprint Allatostatins and allatotropin in the corpus cardiacum/corpus allatum complex of larval and adult lepidopterans studied by confocal laser scanning microscopy: correlation to juvenile hormone biosynthesis
    Hanne Duve
    School of Biological Sciences, Queen Mary, University of London, Mile End Road, E1 4NS, London, UK
    Cell Tissue Res 314:281-95. 2003
    ..The function of this peptide family in the retrocerebral complex remains enigmatic since experiments on JH biosynthesis, either when the peptide is administered alone, or together with Mas-AS, show no effect on JH biosynthesis...
  32. ncbi request reprint Metabolic inactivation of the circadian transmitter, pigment dispersing factor (PDF), by neprilysin-like peptidases in Drosophila
    R Elwyn Isaac
    Faculty of Biological Sciences, University of Leeds, Leeds, UK
    J Exp Biol 210:4465-70. 2007
    ....
  33. doi request reprint Ninth international conference on the juvenile hormones
    Robert J Weaver
    J Insect Physiol 54:893-4. 2008
  34. ncbi request reprint Expression of NEP2, a soluble neprilysin-like endopeptidase, during embryogenesis in Drosophila melanogaster
    Nicholas D Bland
    Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK
    Peptides 28:127-35. 2007
    ..The secretion of NEP2 from tissues during embryogenesis suggests a possible developmental role for this endopeptidase in peptide signalling in D. melanogaster...