Karl Swann

Summary

Affiliation: Cardiff University
Country: UK

Publications

  1. doi request reprint The sperm phospholipase C-ζ and Ca2+ signalling at fertilization in mammals
    Karl Swann
    College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XN, U K
    Biochem Soc Trans 44:267-72. 2016
  2. pmc Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection
    Karl Swann
    School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
    Fertil Steril 97:742-7. 2012
  3. doi request reprint The dynamics of calcium oscillations that activate mammalian eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Int J Dev Biol 52:585-94. 2008
  4. pmc Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility
    Michail Nomikos
    Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom
    Fertil Steril 99:76-85. 2013
  5. ncbi request reprint Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
    J Biol Chem 280:31011-8. 2005
  6. pmc Divergent effect of mammalian PLCζ in generating Ca²⁺ oscillations in somatic cells compared with eggs
    Sally V Phillips
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Biochem J 438:545-53. 2011
  7. pmc PLCζ causes Ca(2+) oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P(2)
    Yuansong Yu
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom
    Mol Biol Cell 23:371-80. 2012
  8. pmc Novel regulation of PLCζ activity via its XY-linker
    Michail Nomikos
    Department of Obstetrics and Gynaecology, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 438:427-32. 2011
  9. pmc Phospholipase Cζ binding to PtdIns(4,5)P2 requires the XY-linker region
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    J Cell Sci 124:2582-90. 2011
  10. doi request reprint Starting a new life: sperm PLC-zeta mobilizes the Ca2+ signal that induces egg activation and embryo development: an essential phospholipase C with implications for male infertility
    Michail Nomikos
    Cell Signalling Laboratory, WHRI, Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff, UK
    Bioessays 34:126-34. 2012

Collaborators

Detail Information

Publications30

  1. doi request reprint The sperm phospholipase C-ζ and Ca2+ signalling at fertilization in mammals
    Karl Swann
    College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XN, U K
    Biochem Soc Trans 44:267-72. 2016
    ..We consider how other regions of PLCζ may mediate its binding to PIP2 in eggs and how interaction of PLCζ with egg-specific factors could enable the hydrolysis of internal sources of PIP2. ..
  2. pmc Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection
    Karl Swann
    School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
    Fertil Steril 97:742-7. 2012
    ..To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca(2+) oscillations during activation...
  3. doi request reprint The dynamics of calcium oscillations that activate mammalian eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Int J Dev Biol 52:585-94. 2008
    ..As yet we do not fully understand how the target of calcium in a mammalian egg might decode the patterns of calcium changes that can occur during egg activation...
  4. pmc Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility
    Michail Nomikos
    Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom
    Fertil Steril 99:76-85. 2013
    ....
  5. ncbi request reprint Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
    J Biol Chem 280:31011-8. 2005
    ..These data suggest that the exquisite Ca2+-dependent features of PLCzeta regulation are essential for it to generate inositol 1,4,5-trisphosphate and Ca2+ oscillations in intact mouse eggs...
  6. pmc Divergent effect of mammalian PLCζ in generating Ca²⁺ oscillations in somatic cells compared with eggs
    Sally V Phillips
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Biochem J 438:545-53. 2011
    ..These data suggest that PLCζ-mediated Ca²⁺ oscillations may require specific factors that are only present within the egg cytoplasm or be inhibited by factors present only in somatic cell lines...
  7. pmc PLCζ causes Ca(2+) oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P(2)
    Yuansong Yu
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom
    Mol Biol Cell 23:371-80. 2012
    ....
  8. pmc Novel regulation of PLCζ activity via its XY-linker
    Michail Nomikos
    Department of Obstetrics and Gynaecology, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 438:427-32. 2011
    ....
  9. pmc Phospholipase Cζ binding to PtdIns(4,5)P2 requires the XY-linker region
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    J Cell Sci 124:2582-90. 2011
    ....
  10. doi request reprint Starting a new life: sperm PLC-zeta mobilizes the Ca2+ signal that induces egg activation and embryo development: an essential phospholipase C with implications for male infertility
    Michail Nomikos
    Cell Signalling Laboratory, WHRI, Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff, UK
    Bioessays 34:126-34. 2012
    ..Recent advances have refined our understanding of how PLCζ induces Ca(2+) oscillations in the egg and also suggest its potential dysfunction as a cause of male infertility...
  11. pmc Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 434:211-7. 2011
    ..Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism...
  12. pmc Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca(2+) oscillations required for mammalian oocyte activation
    Michail Nomikos
    Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Mol Hum Reprod 20:489-98. 2014
    ..Our empirical observations are compatible with a basic mathematical model for the Ca(2+) dependence of generating cytoplasmic Ca(2+) oscillations in mammalian oocytes by sperm PLCζ. ..
  13. pmc Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activation
    Yuansong Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, U K
    Biol Cell 100:633-43. 2008
    ..Fertilization-mediated increase in PKC activity has been demonstrated; however, its precise role is unclear...
  14. pmc Essential Role of the EF-hand Domain in Targeting Sperm Phospholipase Cζ to Membrane Phosphatidylinositol 4,5-Bisphosphate (PIP2)
    Michail Nomikos
    From the Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, United Kingdom and
    J Biol Chem 290:29519-30. 2015
    ....
  15. ncbi request reprint PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development
    Christopher M Saunders
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Development 129:3533-44. 2002
    ..Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo...
  16. pmc Chimeras of sperm PLCζ reveal disparate protein domain functions in the generation of intracellular Ca2+ oscillations in mammalian eggs at fertilization
    Maria Theodoridou
    Institute of Molecular and Experimental Medicine, WHRI, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Mol Hum Reprod 19:852-64. 2013
    ..These numerical simulations can for the first time predict the empirical variability in onset and frequency of Ca(2+) oscillatory activity associated with specific PLC variants. ..
  17. pmc The dynamics of PKC-induced phosphorylation triggered by Ca2+ oscillations in mouse eggs
    Jose Raul Gonzalez-Garcia
    Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff, UK
    J Cell Physiol 228:110-9. 2013
    ..The results also suggest that Ca(2+) -induced DAG formation on intracellular membranes may stimulate PKC activity oscillations at fertilization...
  18. pmc Redistribution of mitochondria leads to bursts of ATP production during spontaneous mouse oocyte maturation
    Yuansong Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK
    J Cell Physiol 224:672-80. 2010
    ....
  19. doi request reprint Use of luciferase chimaera to monitor PLCzeta expression in mouse eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Methods Mol Biol 518:17-29. 2009
    ....
  20. doi request reprint Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation
    Michail Nomikos
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Mol Hum Reprod 21:702-10. 2015
    ..Thus, the functional disparity with PLCζ leads us to conclude that human PAWP is neither sufficient nor necessary for the Ca(2+) oscillations that initiate mammalian oocyte activation at fertilization. ..
  21. pmc Sperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes
    Michail Nomikos
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Mol Hum Reprod 20:938-47. 2014
    ..These data suggest that sperm-derived PAWP is not involved in triggering Ca(2+) oscillations at fertilization in mammalian oocytes...
  22. doi request reprint PLCζ and the initiation of Ca(2+) oscillations in fertilizing mammalian eggs
    Karl Swann
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Cell Calcium 53:55-62. 2013
    ..This leads us to suggest that sperm PLCζ may be targeted to these cytoplasmic vesicles by directly interacting with a specific but as yet unidentified egg PLCζ-binding protein...
  23. doi request reprint PLCζ or PAWP: revisiting the putative mammalian sperm factor that triggers egg activation and embryogenesis
    Junaid Kashir
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK College of Medicine, Alfaisal University, Riyadh, Kingdom of Saudi Arabia Department of Comparative Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia
    Mol Hum Reprod 21:383-8. 2015
    ..Herein, we examine the evidence presented in favour of PAWP in relation to PLCζ and the requisite physiological properties of the mammalian sperm factor. ..
  24. ncbi request reprint Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggs
    Karen Campbell
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
    Dev Biol 298:225-33. 2006
    ..These data suggest that mammalian fertilization is associated with a sudden but transient increase in cytosolic ATP and that Ca(2+) oscillations are both necessary and sufficient to cause this increase in ATP levels...
  25. pmc Rescue of failed oocyte activation after ICSI in a mouse model of male factor infertility by recombinant phospholipase Cζ
    Randa Sanusi
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Mol Hum Reprod 21:783-91. 2015
    ..The data suggest that recombinant human PLCζ protein is an efficient means of rescuing oocyte activation after ICSI failure and that it can be effectively used even if the sperm already contains endogenous Ca(2+) releasing activity...
  26. pmc The dynamics of MAPK inactivation at fertilization in mouse eggs
    Jose Raul Gonzalez-Garcia
    Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
    J Cell Sci 127:2749-60. 2014
    ..These data suggest that the completion of meiosis in mouse zygotes is driven by an increased phosphatase activity and not by a decline in Mos levels or MEK activity. ..
  27. doi request reprint Measuring Ca2+ oscillations in mammalian eggs
    Karl Swann
    School of Medicine, Cardiff University, Heath Park, Cardiff, UK
    Methods Mol Biol 957:231-48. 2013
    ..Either one of these dyes offers advantages over the more commonly used fura2. I describe the way that the fluorescence from dye-loaded eggs is measured with a conventional epifluorescence microscope and a CCD camera...
  28. doi request reprint Sperm-induced Ca2+ release during egg activation in mammals
    Junaid Kashir
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem Biophys Res Commun 450:1204-11. 2014
    ..We discuss the localization of PLCζ within the egg and present a hypothesis about the localization of PLCζ within the sperm before the initiation of fertilization. ..
  29. pmc Is PAWP the "real" sperm factor?
    Michail Nomikos
    Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Asian J Androl 17:444-6. 2015
    ..Here we discuss these new findings and examine the evidence suggesting PAWP as the "real" sperm factor...
  30. doi request reprint Egg Activation at Fertilization by a Soluble Sperm Protein
    Karl Swann
    College of Biomedical and Life Sciences, School of Medicine, Cardiff University, Cardiff, United Kingdom
    Physiol Rev 96:127-49. 2016
    ..We will discuss the role and mechanism of action of PLCζ in detail at a molecular and cellular level. We will also consider some of the evidence that a soluble sperm protein might be involved in egg activation in nonmammalian species. ..