Holger Gerhardt

Summary

Affiliation: Cancer Research UK
Country: UK

Publications

  1. ncbi request reprint Pericytes: gatekeepers in tumour cell metastasis?
    Holger Gerhardt
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    J Mol Med (Berl) 86:135-44. 2008
  2. doi request reprint Endothelial cells dynamically compete for the tip cell position during angiogenic sprouting
    Lars Jakobsson
    Vascular Biology Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Nat Cell Biol 12:943-53. 2010
  3. pmc Endothelial basement membrane limits tip cell formation by inducing Dll4/Notch signalling in vivo
    Denise Stenzel
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    EMBO Rep 12:1135-43. 2011
  4. pmc Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis
    Denise Stenzel
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, London WC2A 3PX, UK
    Development 138:4451-63. 2011
  5. pmc VEGF and Notch in tip and stalk cell selection
    Raquel Blanco
    Vascular Biology Laboratory, London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3LY, UK
    Cold Spring Harb Perspect Med 3:a006569. 2013
  6. pmc Non-canonical Wnt signalling modulates the endothelial shear stress flow sensor in vascular remodelling
    Claudio A Franco
    Vascular Biology Laboratory, Lincoln s Inn Laboratories, London Research Institute, The Francis Crick Institute, London, United Kingdom
    elife 5:e07727. 2016
  7. doi request reprint VEGFRs and Notch: a dynamic collaboration in vascular patterning
    Lars Jakobsson
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem Soc Trans 37:1233-6. 2009
  8. doi request reprint Peripheral mural cell recruitment requires cell-autonomous heparan sulfate
    Denise Stenzel
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London, United Kingdom
    Blood 114:915-24. 2009
  9. doi request reprint Vascular morphogenesis: a Wnt for every vessel?
    Claudio Areias Franco
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, WC2A 3PX London, UK
    Curr Opin Genet Dev 19:476-83. 2009
  10. ncbi request reprint Agent-based simulation of notch-mediated tip cell selection in angiogenic sprout initialisation
    Katie Bentley
    Biomolecular Modelling Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    J Theor Biol 250:25-36. 2008

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Pericytes: gatekeepers in tumour cell metastasis?
    Holger Gerhardt
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    J Mol Med (Berl) 86:135-44. 2008
    ..In this paper, we review recent evidence for a novel role of the supporting mural cells in limiting blood-borne metastasis...
  2. doi request reprint Endothelial cells dynamically compete for the tip cell position during angiogenic sprouting
    Lars Jakobsson
    Vascular Biology Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Nat Cell Biol 12:943-53. 2010
    ..The regular exchange of the leading tip cell raises novel implications for the concept of guided angiogenic sprouting...
  3. pmc Endothelial basement membrane limits tip cell formation by inducing Dll4/Notch signalling in vivo
    Denise Stenzel
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    EMBO Rep 12:1135-43. 2011
    ..We conclude that appropriate laminin/integrin-induced signalling is necessary to induce physiologically functional levels of Dll4 expression and regulate branching frequency during sprouting angiogenesis in vivo...
  4. pmc Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis
    Denise Stenzel
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, London WC2A 3PX, UK
    Development 138:4451-63. 2011
    ..We propose that in vivo the VEGF-binding properties of FN and HS promote directional tip cell migration, whereas FN integrin-binding functions to support filopodia adhesion to the astrocytic migration template...
  5. pmc VEGF and Notch in tip and stalk cell selection
    Raquel Blanco
    Vascular Biology Laboratory, London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3LY, UK
    Cold Spring Harb Perspect Med 3:a006569. 2013
    ..In this article, we summarize the current knowledge and implications of the tip/stalk cell concepts and the quantitative and dynamic integration of VEGF and Notch signaling in tip and stalk cell selection...
  6. pmc Non-canonical Wnt signalling modulates the endothelial shear stress flow sensor in vascular remodelling
    Claudio A Franco
    Vascular Biology Laboratory, Lincoln s Inn Laboratories, London Research Institute, The Francis Crick Institute, London, United Kingdom
    elife 5:e07727. 2016
    ..Collectively our data suggest that non-canonical Wnt signalling stabilizes forming vascular networks by reducing endothelial shear sensitivity, thus keeping vessels open under low flow conditions that prevail in the primitive plexus...
  7. doi request reprint VEGFRs and Notch: a dynamic collaboration in vascular patterning
    Lars Jakobsson
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem Soc Trans 37:1233-6. 2009
    ....
  8. doi request reprint Peripheral mural cell recruitment requires cell-autonomous heparan sulfate
    Denise Stenzel
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London, United Kingdom
    Blood 114:915-24. 2009
    ..Once MCs are in contact with the vessel, as during CNS vascularization, endothelial HS appears sufficient to facilitate PDGFR-beta activation in trans...
  9. doi request reprint Vascular morphogenesis: a Wnt for every vessel?
    Claudio Areias Franco
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, WC2A 3PX London, UK
    Curr Opin Genet Dev 19:476-83. 2009
    ..Here, we review the emerging concepts of the molecular actions of Wnt-signalling in regulating differential behaviour and/or cell functions during vascular development...
  10. ncbi request reprint Agent-based simulation of notch-mediated tip cell selection in angiogenic sprout initialisation
    Katie Bentley
    Biomolecular Modelling Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    J Theor Biol 250:25-36. 2008
    ....
  11. pmc Tipping the balance: robustness of tip cell selection, migration and fusion in angiogenesis
    Katie Bentley
    Biomolecular Modelling Laboratory, Cancer Research UK London Research Institute, London, United Kingdom
    PLoS Comput Biol 5:e1000549. 2009
    ..Thus we suggest the use of migration inhibitors as therapeutic agents for vascular normalisation in cancer...
  12. doi request reprint Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo
    Véronique Gebala
    The Francis Crick Institute, Lincoln s Inn Fields Laboratory, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Nat Cell Biol 18:443-50. 2016
    ..We show that in the case of blood vessels such membrane dynamics can drive local cell shape changes required for global tissue morphogenesis, shedding light on a pressure-driven mechanism of lumen formation in vertebrates. ..
  13. pmc Alk1 and Alk5 inhibition by Nrp1 controls vascular sprouting downstream of Notch
    Irene Maria Aspalter
    Vascular Biology Laboratory, London Research Institute, Cancer Research UK, London WC2A 3LY, UK
    Nat Commun 6:7264. 2015
    ....
  14. pmc Imaging transient blood vessel fusion events in zebrafish by correlative volume electron microscopy
    Hannah E J Armer
    Electron Microscopy Unit, London Research Institute, Cancer Research UK, London, UK
    PLoS ONE 4:e7716. 2009
    ..The resulting data give unprecedented microanatomical detail of the zebrafish and, for the first time, allow visualization of the ultrastructure of a time-limited biological event within the context of a whole organism...
  15. doi request reprint Nrarp coordinates endothelial Notch and Wnt signaling to control vessel density in angiogenesis
    Li Kun Phng
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, London, UK
    Dev Cell 16:70-82. 2009
    ..In vivo, loss of Nrarp, Lef1, or endothelial Ctnnb1 causes vessel regression. We suggest that the balance between Notch and Wnt signaling determines whether to make or break new vessel connections...
  16. pmc Dynamic endothelial cell rearrangements drive developmental vessel regression
    Claudio A Franco
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, Lincoln s Inn Laboratories, London, United Kingdom Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa, Lisboa, Portugal
    PLoS Biol 13:e1002125. 2015
    ..We predict that flow-induced polarized migration of endothelial cells breaks symmetry and leads to stabilization of high flow/shear segments and regression of adjacent low flow/shear segments. ..
  17. ncbi request reprint Growth factor gradients in vascular patterning
    Andrea Lundkvist
    Vascular Biology Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Novartis Found Symp 283:194-201; discussion 201-6, 238-41. 2007
    ..Disruption of VEGF-A gradients provides a novel concept for the mechanism underlying pathological patterning in ocular disease...
  18. pmc VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia
    Holger Gerhardt
    Deparrment of Medical Biochemistry, University of Goteborg, Medicinaregatan 9A, Box 440, SE 405 30 Goteborg, Sweden
    J Cell Biol 161:1163-77. 2003
    ..Thus, vessel patterning during retinal angiogenesis depends on the balance between two different qualities of the extracellular VEGF-A distribution, which regulate distinct cellular responses in defined populations of endothelial cells...
  19. doi request reprint Angiogenesis selectively requires the p110alpha isoform of PI3K to control endothelial cell migration
    Mariona Graupera
    Centre for Cell Signalling, Institute of Cancer, Queen Mary, University of London, Charterhouse Square, London EC1M 6BQ, UK
    Nature 453:662-6. 2008
    ..These results provide the first in vivo evidence for p110-isoform selectivity in endothelial PI3K signalling during angiogenesis...
  20. pmc Pericytes limit tumor cell metastasis
    Xiaojie Xian
    Stem Cell Center, Lund University, Lund, Sweden
    J Clin Invest 116:642-51. 2006
    ..These data support a new model for how tumor cells trigger metastasis by perturbing pericyte-endothelial cell-cell interactions...
  21. pmc Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall
    Per Lindblom
    Department of Medical Biochemistry, Goteborg University, Göteborg SE 405 30, Sweden
    Genes Dev 17:1835-40. 2003
    ..We conclude that retention of PDGF-B in microvessels is essential for proper recruitment and organization of pericytes and for renal and retinal function in adult mice...
  22. ncbi request reprint Therapeutic antibodies targeting angiomotin inhibit angiogenesis in vivo
    Tetyana Levchenko
    Department of Oncology and Pathology, Cancer Centre Karolinska, Karolinska Institute, SE 17176, Stockholm, Sweden
    FASEB J 22:880-9. 2008
    ..These findings provide a rationale for using angiomotin antibodies for specifically targeting endothelial migration in angiogenesis-dependent diseases...
  23. ncbi request reprint Neuropilin-1 is required for endothelial tip cell guidance in the developing central nervous system
    Holger Gerhardt
    Department of Medical Biochemistry, University of Goteborg, Medicinaregatan 9A, Box 440, SE 405 30 Goteborg, Sweden
    Dev Dyn 231:503-9. 2004
    ..These observations suggest that Nrp-1 plays an important role in allowing the endothelial tip cell filopodia to switch substrate and protrude in a new direction at a specific location in the developing brain...
  24. pmc Defective N-sulfation of heparan sulfate proteoglycans limits PDGF-BB binding and pericyte recruitment in vascular development
    Alexandra Abramsson
    Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Genes Dev 21:316-31. 2007
    ....
  25. doi request reprint Dendritic cell expression of the Notch ligand jagged2 is not essential for Th2 response induction in vivo
    Alan G F Worsley
    Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    Eur J Immunol 38:1043-9. 2008
    ..These data suggest that, although involved in Th2 direction in vitro, jagged2 is not fundamentally required for Th2 induction by SEA-activated dendritic cells in vivo...
  26. pmc Defective associations between blood vessels and brain parenchyma lead to cerebral hemorrhage in mice lacking alphav integrins
    Joseph H McCarty
    Howard Hughes Medical Institute and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Cell Biol 22:7667-77. 2002
    ..These data suggest a novel role for alphav integrins in the association between cerebral microvessels and central nervous system parenchymal cells...
  27. pmc Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis
    Christiana Ruhrberg
    Endothelial Cell Biology Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, United Kingdom
    Genes Dev 16:2684-98. 2002
    ..We conclude that differential VEGF-A isoform localization in the extracellular space provides a control point for regulating vascular branching pattern...
  28. ncbi request reprint Endothelial-pericyte interactions in angiogenesis
    Holger Gerhardt
    Department of Medical Biochemistry, University of Goteborg, Medicinaregatan 9A, PO Box 440, SE 405 30, Goteborg, Sweden
    Cell Tissue Res 314:15-23. 2003
    ..Genetic data demonstrate the critical importance of pericytes for vascular morphogenesis and function, and imply specific roles for the cell type in various aspects of angiogenesis...
  29. ncbi request reprint Cortical and retinal defects caused by dosage-dependent reductions in VEGF-A paracrine signaling
    Jody J Haigh
    Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Canada
    Dev Biol 262:225-41. 2003
    ..VEGF-A dosage is therefore a critical parameter regulating the density of the vascular plexus in the developing CNS that is in turn a key determinant in the development and architectural organization of the nervous system...
  30. ncbi request reprint Role of platelet-derived growth factor in mesangium development and vasculopathies: lessons from platelet-derived growth factor and platelet-derived growth factor receptor mutations in mice
    Christer Betsholtz
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Curr Opin Nephrol Hypertens 13:45-52. 2004
    ....
  31. ncbi request reprint Unique vascular phenotypes following over-expression of individual VEGFA isoforms from the developing lens
    Christopher A Mitchell
    Department of Obstetrics and Gynaecology, University of Nottingham, City Hospital, Hucknall Rd, Nottingham, NG5 1PB, UK
    Angiogenesis 9:209-24. 2006
    ..The results of this study indicate that individual isoforms of VEGFA induce distinct vascular phenotypes in the eye during embryonic development and that their relative doses provide instructive cues for vascular patterning...
  32. ncbi request reprint Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis
    Mats Hellstrom
    AngioGenetics Sweden AB, Scheeles vag 2, SE 171 77 Stockholm, Sweden
    Nature 445:776-80. 2007
    ....
  33. pmc Robo4 stabilizes the vascular network by inhibiting pathologic angiogenesis and endothelial hyperpermeability
    Christopher A Jones
    Department of Oncological Sciences, University of Utah, 15 North 2030 East, Salt Lake City, Utah 84112, USA
    Nat Med 14:448-53. 2008
    ....
  34. pmc Endothelium-specific platelet-derived growth factor-B ablation mimics diabetic retinopathy
    Maria Enge
    Department of Medical Biochemistry, Goteborg University, PO Box 440, SE 405 30 Goteborg, Sweden
    EMBO J 21:4307-16. 2002
    ..Our data suggest that a reduction of the pericyte density is sufficient to cause retinopathy in mice, implying that pericyte loss may also be a causal pathogenic event in human diabetic retinopathy...