Paul J M Wijnker

Summary

Affiliation: VU University Medical Center
Country: The Netherlands

Publications

  1. pmc Troponin I phosphorylation in human myocardium in health and disease
    P J M Wijnker
    Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Van der Boechorststraat 7, 1081, BT, Amsterdam, The Netherlands
    Neth Heart J 22:463-9. 2014
  2. pmc A novel phosphorylation site, Serine 199, in the C-terminus of cardiac troponin I regulates calcium sensitivity and susceptibility to calpain-induced proteolysis
    Paul J M Wijnker
    Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg Eppendorf, Hamburg, Germany Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands Electronic address
    J Mol Cell Cardiol 82:93-103. 2015
  3. pmc Phosphorylation of protein kinase C sites Ser42/44 decreases Ca(2+)-sensitivity and blunts enhanced length-dependent activation in response to protein kinase A in human cardiomyocytes
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg Eppendorf, Hamburg, Germany Electronic address
    Arch Biochem Biophys 554:11-21. 2014
  4. pmc Impact of site-specific phosphorylation of protein kinase A sites Ser23 and Ser24 of cardiac troponin I in human cardiomyocytes
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Am J Physiol Heart Circ Physiol 304:H260-8. 2013
  5. pmc Protein phosphatase 2A affects myofilament contractility in non-failing but not in failing human myocardium
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands
    J Muscle Res Cell Motil 32:221-33. 2011
  6. pmc Length-dependent activation is modulated by cardiac troponin I bisphosphorylation at Ser23 and Ser24 but not by Thr143 phosphorylation
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Am J Physiol Heart Circ Physiol 306:H1171-81. 2014
  7. pmc PKCα-specific phosphorylation of the troponin complex in human myocardium: a functional and proteomics analysis
    Viola Kooij
    Laboratory for Physiology, Institute for Cardiovascular Research, VU Medical Center, Amsterdam, The Netherlands Johns Hopkins Bayview Proteomics Center, Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 8:e74847. 2013
  8. pmc Perturbed length-dependent activation in human hypertrophic cardiomyopathy with missense sarcomeric gene mutations
    Vasco Sequeira
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Circ Res 112:1491-505. 2013
  9. pmc ADP-stimulated contraction: A predictor of thin-filament activation in cardiac disease
    Vasco Sequeira
    Department of Physiology, Institute for Cardiovascular Research, Vrije Universiteit Medisch Centrum, 1081BT Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 112:E7003-12. 2015
  10. doi Comparison of the effects of a truncating and a missense MYBPC3 mutation on contractile parameters of engineered heart tissue
    Paul J M Wijnker
    Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg Eppendorf, Hamburg, Germany DZHK German Centre for Cardiovascular Research, partner site Hamburg Kiel Lübeck, Germany Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    J Mol Cell Cardiol 97:82-92. 2016

Collaborators

Detail Information

Publications10

  1. pmc Troponin I phosphorylation in human myocardium in health and disease
    P J M Wijnker
    Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Van der Boechorststraat 7, 1081, BT, Amsterdam, The Netherlands
    Neth Heart J 22:463-9. 2014
    ..Moreover, we provide an overview of recent studies which aimed to reveal the functional consequences of changes in cTnI phosphorylation in cardiac disease. ..
  2. pmc A novel phosphorylation site, Serine 199, in the C-terminus of cardiac troponin I regulates calcium sensitivity and susceptibility to calpain-induced proteolysis
    Paul J M Wijnker
    Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg Eppendorf, Hamburg, Germany Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands Electronic address
    J Mol Cell Cardiol 82:93-103. 2015
    ..In addition, cTnI-Ser199 pseudo-phosphorylation or mutation regulates calpain I mediated proteolysis of cTnI...
  3. pmc Phosphorylation of protein kinase C sites Ser42/44 decreases Ca(2+)-sensitivity and blunts enhanced length-dependent activation in response to protein kinase A in human cardiomyocytes
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg Eppendorf, Hamburg, Germany Electronic address
    Arch Biochem Biophys 554:11-21. 2014
    ..Since the drop in Ca(2+)-sensitivity at physiological Ca(2+)-concentrations is relatively large phosphorylation of Ser42/44 may result in a decrease of force and associated ATP utilization in the human heart...
  4. pmc Impact of site-specific phosphorylation of protein kinase A sites Ser23 and Ser24 of cardiac troponin I in human cardiomyocytes
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Am J Physiol Heart Circ Physiol 304:H260-8. 2013
    ..The implications for in vivo cardiac function in health and disease are detailed in the DISCUSSION in this article...
  5. pmc Protein phosphatase 2A affects myofilament contractility in non-failing but not in failing human myocardium
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands
    J Muscle Res Cell Motil 32:221-33. 2011
    ..This effect is not present in failing hearts, probably due to the lower baseline cTnI phosphorylation in failing compared to non-failing hearts...
  6. pmc Length-dependent activation is modulated by cardiac troponin I bisphosphorylation at Ser23 and Ser24 but not by Thr143 phosphorylation
    Paul J M Wijnker
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Am J Physiol Heart Circ Physiol 306:H1171-81. 2014
    ..Thus phosphorylation at Thr143 enhances myofilament Ca(2+) sensitivity without affecting LDA, while Ser23/24 bisphosphorylation is needed to enhance the length-dependent increase in myofilament Ca(2+) sensitivity...
  7. pmc PKCα-specific phosphorylation of the troponin complex in human myocardium: a functional and proteomics analysis
    Viola Kooij
    Laboratory for Physiology, Institute for Cardiovascular Research, VU Medical Center, Amsterdam, The Netherlands Johns Hopkins Bayview Proteomics Center, Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 8:e74847. 2013
    ..This study aimed to investigate the effects of PKCα phosphorylation of cardiac troponin (cTn) on myofilament function in human failing cardiomyocytes and to resolve the potential targets involved...
  8. pmc Perturbed length-dependent activation in human hypertrophic cardiomyopathy with missense sarcomeric gene mutations
    Vasco Sequeira
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Circ Res 112:1491-505. 2013
    ..However, myofilament Ca(2+) sensitivity depends on protein phosphorylation and muscle length, and at present, data in humans are scarce...
  9. pmc ADP-stimulated contraction: A predictor of thin-filament activation in cardiac disease
    Vasco Sequeira
    Department of Physiology, Institute for Cardiovascular Research, Vrije Universiteit Medisch Centrum, 1081BT Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 112:E7003-12. 2015
    ..In the presence of Ca(2+), pathologic [ADP] and low PKA-phosphorylation, high actin-myosin formation could contribute to the impaired myocardial relaxation observed in cardiomyopathies. ..
  10. doi Comparison of the effects of a truncating and a missense MYBPC3 mutation on contractile parameters of engineered heart tissue
    Paul J M Wijnker
    Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg Eppendorf, Hamburg, Germany DZHK German Centre for Cardiovascular Research, partner site Hamburg Kiel Lübeck, Germany Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    J Mol Cell Cardiol 97:82-92. 2016
    ..g. sarcomere poisoning for the missense mutation, iii) the direct impact of (newly identified) MYBPC3 gene variants can be evaluated. ..